{"title":"Cell culture reagents","description":"\u003cp\u003eHere you will find reagents and accessories supporting all types of cell culture applications:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/serum-replacement\" title=\"Serum replacements\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/serum-replacement\"\u003eSerum Replacements\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/amino-acids-vitamins-cell-culture\" title=\"Amino Acids and Vitamins product collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/amino-acids-vitamins-cell-culture\"\u003eAmino Acids and Vitamins\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/antibiotics-and-antifungal-drugs-for-cell-culture\" title=\"Antibiotics and antifungal drugs for cell culture\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/antibiotics-and-antifungal-drugs-for-cell-culture\"\u003eAntibiotics and Antifungal Drugs\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/attachment-factors-cell-culture\" title=\"Attachment Factors Product Collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/attachment-factors-cell-culture\"\u003eAttachment Factors\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/balanced-salt-solutions\" title=\"Balanced Salt Solutions product collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/balanced-salt-solutions\"\u003eBalanced Salt Solutions\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" title=\"Bovine Serum Albumin (BSA) and Human Serum Albumin (HSA) Product Collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\"\u003eBSA \/ HSA\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/cryopreservation-media\" title=\"Cryopreservation media product collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/cryopreservation-media\"\u003eCryopreservation media\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/cytokines\" title=\"Cytokines \u0026amp; Growth Factors product collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/cytokines\"\u003eCytokines and Growth Factors\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/enzymes-for-cell-dissociation\" title=\"Enzymes for Cell Dissociation\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/enzymes-for-cell-dissociation\"\u003eEnzymes for Cell Dissociation\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/collections\/fbs-fetal-bovine-serum\" title=\"FBS (Fetal Bovine Serum)\" rel=\"noopener noreferrer\" target=\"_blank\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/fbs-fetal-bovine-serum\"\u003eFBS (Fetal Bovine Serum)\u003c\/a\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/amino-acids-vitamins-cell-culture\" title=\"Amino Acids and Vitamins product collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/amino-acids-vitamins-cell-culture\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/human-serum-human-platelet-lysate\" title=\"Human Serum \u0026amp;amp; Human Platelet Lysate (hPL) Product Collection\"\u003eHuman Serum \u0026amp; Human Platelet Lysate (hPL)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/cell-culture-media-supplements\" title=\"Media Supplements product collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/cell-culture-media-supplements\"\u003eMedia Supplements\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/cell-separating-solutions\" title=\"Cell Separating Solutions\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/cell-separating-solutions\"\u003eSeparating Solutions\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003eAntibodies\u003c\/li\u003e\n\u003c\/ul\u003e","products":[{"product_id":"biocolor-blyscan-glycosaminoglycan-assay","title":"Biocolor Blyscan™ Glycosaminoglycan Assay","description":"\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eDiscover the Blyscan™ Glycosaminoglycan Kit: Your go-to Solution for reliable sGAG and Proteoglycan Analysis!\u003c\/strong\u003e Our versatile, widely cited kit utilizes a dye-binding approach to quantitatively measure sulfated glycosaminoglycans (sGAG) and proteoglycans in cells, tissues, and fluids from in-vivo and in-vitro sources.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe Biocolor \u003cstrong\u003eBlyscan™ Glycosaminoglycan (sGAG) Assay\u003c\/strong\u003e enables quantitative measurement and analysis of sulfated proteoglycans and glycosaminoglycans (sGAG).\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eSoluble sGAGs may be assayed directly, whereas solid materials will require a preliminary papain extraction.\u003c\/p\u003e\n\u003cp\u003eThe assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples.\u003c\/p\u003e\n\u003ch2\u003eSuitable Samples\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eIn-vivo:\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolid samples,\u003c\/strong\u003e including cartilage, bone, connective tissue, tumor tissue\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLiquid samples\u003c\/strong\u003e, including fluids such as urine, amniotic fluid, or synovial fluid\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eIn-vitro:\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolid samples,\u003c\/strong\u003e such as deposited ECM on 2D\/3D culture surfaces by enzymatic treatment\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLiquid samples,\u003c\/strong\u003e Culture media during 2D\/3D cell culture\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe assay requires that sulfated polysaccharides or sGAGs are in a soluble form. A preliminary enzymatic extraction step is required for solid samples (enzyme not supplied with kit).\u003c\/p\u003e\n\u003cp\u003eThe assay is not suitable for use with samples containing alginates or that comprise degraded sulfated disaccharide fragments.\u003c\/p\u003e\n\u003ch2\u003ePrinciple of Method\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eColorimetric Assay, Dimethylmethylene Blue Assay (DMMB)\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThe dye label used in the assay is 1,9-dimethylmethylene blue (DMMB) and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 1.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eBlyscan dye reagent contains DMMB dye in an optimized buffer. Addition of Dye reagent to samples containing sGAG results in the formation of a dye\/sGAG complex due to a charge interaction between dye and GAG sulfate groups.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 2.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eOver a 30 minute incubation Dye-labelled sGAGs precipitate out of solution and are collected by centrifugation. Following removal of unbound dye, the remaining bound dye is released from the complex by addition of dye dissociation reagent. Released dye is quantified spectrophotometrically.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 3.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eThe sGAG content of unknown samples may be quantified by comparison against a calibration curve prepared using a standard of purified Chondroitin-4-sulfate supplied with the kit.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003e\u003cem\u003eThe Blyscan Dye reagent is formulated to minimize binding to other charged sample components such as nucleic acids, a problem with some older dye-based sGAG assays.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"Biocolor Blyscan sGAG 1,9-dimethylmethylene blue dye\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Blyscan-color-plate-photo_480x480.jpg?v=1665086674\"\u003e\u003c\/p\u003e\n\u003ch2\u003eTest Sample Composition\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eAn extensive range of mammalian proteoglycans have been measured using this assay. The following sGAG, either still attached to the peptide\/protein core, or as free chains can be assayed:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003echondroitin sulfates (4- \u0026amp; 6-sulfated)\u003c\/li\u003e\n\u003cli\u003ekeratan sulfates (alkali sensitive \u0026amp; resistant forms)\u003c\/li\u003e\n\u003cli\u003edermatan sulfate\u003c\/li\u003e\n\u003cli\u003eheparan sulfates (including heparins)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eHyaluronate is not detected by the Blyscan Assay. The presence of hyaluronate in test samples does not interfere with the measurement of sGAG.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Category:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003ca aria-describedby=\"a11y-new-window-message\" title=\"Browse Extracellular Matrix Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cstrong\u003eDetection Method:\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eColorimetric Detection (656 nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cstrong\u003eAssay Range:\u003c\/strong\u003e\u003cspan style=\"border-block: unset; border-inline: unset; border-start-start-radius: unset; border-start-end-radius: unset; border-end-start-radius: unset; border-end-end-radius: unset; overflow-inline: unset; overflow-block: unset; overscroll-behavior-inline: unset; overscroll-behavior-block: unset; margin-block: unset; margin-inline: unset; scroll-margin-block: unset; scroll-margin-inline: unset; padding-block: unset; padding-inline: unset; scroll-padding-block: unset; scroll-padding-inline: unset; inset-block: unset; inset-inline: unset; block-size: unset; min-block-size: unset; max-block-size: unset; inline-size: unset; min-inline-size: unset; max-inline-size: unset; contain-intrinsic-block-size: unset; contain-intrinsic-inline-size: unset; background: unset; background-blend-mode: unset; border: unset; border-radius: unset; box-decoration-break: unset; -moz-float-edge: unset; display: unset; position: fixed; float: unset; clear: unset; vertical-align: unset; overflow: unset; overflow-anchor: unset; transform: unset; rotate: unset; scale: unset; translate: unset; offset: unset; scroll-behavior: unset; scroll-snap-align: unset; scroll-snap-type: unset; scroll-snap-stop: unset; overscroll-behavior: unset; isolation: unset; break-after: unset; break-before: unset; break-inside: unset; resize: unset; perspective: unset; perspective-origin: unset; backface-visibility: unset; transform-box: unset; transform-style: unset; transform-origin: unset; contain: unset; appearance: unset; -moz-orient: unset; will-change: unset; shape-image-threshold: unset; shape-margin: unset; shape-outside: unset; touch-action: unset; -webkit-line-clamp: unset; scrollbar-gutter: unset; columns: unset; column-fill: unset; column-rule: unset; column-span: unset; content: unset; counter-increment: unset; counter-reset: unset; counter-set: unset; opacity: unset; box-shadow: unset; clip: rect(0px, 0px, 0px, 0px); filter: unset; backdrop-filter: unset; mix-blend-mode: unset; font: unset; font-synthesis: unset; visibility: unset; writing-mode: unset; text-orientation: unset; print-color-adjust: unset; image-rendering: unset; image-orientation: unset; dominant-baseline: unset; text-anchor: unset; color-interpolation: unset; color-interpolation-filters: unset; fill: unset; fill-opacity: unset; fill-rule: unset; shape-rendering: unset; stroke: unset; stroke-width: unset; stroke-linecap: unset; stroke-linejoin: unset; stroke-miterlimit: unset; stroke-opacity: unset; stroke-dasharray: unset; stroke-dashoffset: unset; clip-rule: unset; marker: unset; paint-order: unset; border-collapse: unset; empty-cells: unset; caption-side: unset; border-spacing: unset; color: unset; text-transform: unset; hyphens: unset; -moz-text-size-adjust: unset; text-indent: unset; overflow-wrap: unset; word-break: unset; text-justify: unset; text-align-last: unset; text-align: unset; letter-spacing: unset; word-spacing: unset; white-space: pre; text-shadow: unset; text-emphasis: unset; text-emphasis-position: unset; tab-size: unset; line-break: unset; -webkit-text-fill-color: unset; -webkit-text-stroke: unset; ruby-align: unset; ruby-position: unset; text-combine-upright: unset; text-rendering: unset; text-underline-offset: unset; text-underline-position: unset; text-decoration-skip-ink: unset; hyphenate-character: unset; cursor: unset; pointer-events: unset; -moz-user-input: unset; -moz-user-modify: unset; -moz-user-focus: unset; caret-color: unset; accent-color: unset; color-scheme: unset; scrollbar-color: unset; list-style: unset; quotes: unset; -moz-image-region: unset; margin: unset; overflow-clip-margin: unset; scroll-margin: unset; outline: unset; outline-offset: unset; size: unset; padding: unset; scroll-padding: unset; top: 0px; right: unset; bottom: unset; left: unset; z-index: unset; flex-flow: unset; place-content: unset; place-items: unset; flex: unset; place-self: unset; order: unset; width: unset; min-width: unset; max-width: unset; height: unset; min-height: unset; max-height: unset; box-sizing: unset; object-fit: unset; object-position: unset; grid-area: unset; grid: unset; gap: unset; aspect-ratio: unset; contain-intrinsic-size: unset; vector-effect: unset; stop-color: unset; stop-opacity: unset; flood-color: unset; flood-opacity: unset; lighting-color: unset; mask-type: unset; clip-path: unset; mask: unset; x: unset; y: unset; cx: unset; cy: unset; rx: unset; ry: unset; r: unset; d: unset; table-layout: unset; text-overflow: unset; text-decoration: unset; ime-mode: unset; scrollbar-width: unset; user-select: text; -moz-window-dragging: unset; -moz-force-broken-image-icon: unset; transition: unset; animation: unset; -moz-box-align: unset; -moz-box-direction: unset; -moz-box-flex: unset; -moz-box-orient: unset; -moz-box-pack: unset; -moz-box-ordinal-group: unset;\"\u003ehttps:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Blyscan-manual-webopt.pdf?v=1672706553\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e0 - 50 µg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLimit of Detection:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2.5 µg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.5 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAssay Run-Time:\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMeasurements Per Kit:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e110 in total (allows a maximum of 48 samples to be run in duplicate alongside a standard curve)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage (unopened kit):\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRoom Temperature (RT)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eBiocolor Ltd.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCountry of Origin:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eUnited Kingdom\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch2\u003eKit Contents\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe standard size Blyscan™ Glycosaminoglycan kit (cat. no. B1000) contains:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eBlyscan Dye Reagent (1x 110 ml)\u003c\/li\u003e\n\u003cli\u003esGAG Reference Standard (1x 5 ml, 100 µg\/ml Bovine tracheal chondroitin 4-sulfate)\u003c\/li\u003e\n\u003cli\u003eDissociation Reagent (1x 110 ml)\u003c\/li\u003e\n\u003cli\u003eSodium Nitrite (1x 15 ml)\u003c\/li\u003e\n\u003cli\u003eAcetic Acid (1x 15 ml)\u003c\/li\u003e\n\u003cli\u003eAmmonium Sulfamate (1x 15 ml)\u003c\/li\u003e\n\u003cli\u003e1.5 ml micro-centrifuge tubes for dye-labelling reaction\u003c\/li\u003e\n\u003cli\u003eAssay kit manual\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cem\u003eNOTE: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details.\u003c\/em\u003e\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003efibrous and hyaline cartilages\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003earteries, heart, lung, skin and other material containing extracellular matrix (connective tissue), and solid tumors\u003c\/li\u003e\n\u003cli\u003eextracellular matrix components that may be released by live cells into culture medium\u003c\/li\u003e\n\u003cli\u003esoluble sGAG from synovial fluid, urine and gel chromatography fraction aliquots\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eTechnical Video\u003c\/h2\u003e\n\u003cp\u003e\u003ciframe height=\"315\" width=\"560\" title=\"YouTube video player\" src=\"https:\/\/www.youtube.com\/embed\/VQbMCzrmXSk\"\u003e\u003c\/iframe\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\/Synonyms\u003c\/h2\u003e\n\u003cp\u003eBlyscan sGAG Assay, s-glycosaminoglycan, sulfated glycosaminoglycan\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Blyscan_Glycosaminoglycan_sGAG_Assay_Manual.pdf?v=1707152889\" title=\"Product Datasheet (PDS): Biocolor Blyscan™ Glycosaminoglycan Assay Manual, Biocolor Blyscan™ Glycosaminoglycan Assay Protocol\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Blyscan-sGAG-Assay-Kit-sds-en-eu-v9.pdf?v=1628197830\" title=\"Material Safety Data Sheet (MSDS) for Biocolor Blyscan™ Glycosaminoglycan Assay \u0026amp; Components\" target=\"_blank\"\u003e\u003cspan\u003eMaterial Safety Datasheet (MSDS)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Biocolor Blyscan™ Glycosaminoglycan (sGAG) Assay - Frequently Asked Questions (FAQ)\" href=\"https:\/\/ilexlife.com\/pages\/biocolor-blyscan-glycosaminoglycan-sgag-assay-frequently-asked-questions-faq\" target=\"_blank\"\u003e\u003cspan\u003eFrequently Asked Questions (FAQ)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e2025 Product References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eZhou, Jialiang et al. “Decellularized Adipose Matrix Rejuvenates Photoaged Skin through Immune Microenvironment Modulation.” \u003ci\u003eBME frontiers\u003c\/i\u003e vol. 6 0166. 4 Aug. 2025, d\u003ca rel=\"noopener\" title=\"Zhou, Jialiang et al. “Decellularized Adipose Matrix Rejuvenates Photoaged Skin through Immune Microenvironment Modulation.” BME frontiers vol. 6 0166. 4 Aug. 2025, doi:10.34133\/bmef.0166\" href=\"https:\/\/doi.org\/10.34133\/bmef.0166\" target=\"_blank\"\u003eoi:10.34133\/bmef.0166\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eHussein, Kamal H et al. “Efficacy of xenogeneic fresh and lyophilized amniotic membranes on the healing of experimentally induced full-thickness skin wounds in dogs.” \u003c\/span\u003e\u003ci\u003eScientific reports\u003c\/i\u003e\u003cspan\u003e vol. 15,1 15605. 4 May. 2025, \u003ca rel=\"noopener\" title=\"Hussein, Kamal H et al. “Efficacy of xenogeneic fresh and lyophilized amniotic membranes on the healing of experimentally induced full-thickness skin wounds in dogs.” Scientific reports vol. 15,1 15605. 4 May. 2025, doi:10.1038\/s41598-025-95023-9\" href=\"https:\/\/doi.org\/10.1038\/s41598-025-95023-9\" target=\"_blank\"\u003edoi:10.1038\/s41598-025-95023-9\u003c\/a\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBhattacharyya, Sumit, and Joanne K Tobacman. “Interactions of CFTR and Arylsulfatase B (ARSB; N-acetylgalactosamine-4-sulfatase) in Prostate Carcinoma.” \u003ci\u003eInternational journal of molecular sciences\u003c\/i\u003e vol. 26,9 4350. 3 May. 2025, \u003ca href=\"https:\/\/doi.org\/10.3390\/ijms26094350\" title=\"Bhattacharyya, Sumit, and Joanne K Tobacman. “Interactions of CFTR and Arylsulfatase B (ARSB; N-acetylgalactosamine-4-sulfatase) in Prostate Carcinoma.” International journal of molecular sciences vol. 26,9 4350. 3 May. 2025, doi:10.3390\/ijms26094350\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.3390\/ijms26094350\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBreland, Austen N et al. “In Silico Insights into the Inhibition of ADAMTS-5 by Punicalagin and Ellagic Acid for the Treatment of Osteoarthritis.” \u003ci\u003eInternational journal of molecular sciences\u003c\/i\u003e vol. 26,9 4093. 25 Apr. 2025, \u003ca rel=\"noopener\" title=\"Breland, Austen N et al. “In Silico Insights into the Inhibition of ADAMTS-5 by Punicalagin and Ellagic Acid for the Treatment of Osteoarthritis.” International journal of molecular sciences vol. 26,9 4093. 25 Apr. 2025, doi:10.3390\/ijms26094093\" href=\"https:\/\/doi.org\/10.3390\/ijms26094093\" target=\"_blank\"\u003edoi:10.3390\/ijms26094093\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eShinoda, Chika et al. “N-glycan-modified α-L-iduronidase produced by transgenic silkworms ameliorates clinical signs in a Japanese macaque with mucopolysaccharidosis I.” \u003ci\u003eCommunications medicine\u003c\/i\u003e vol. 5,1 128. 18 Apr. 2025, \u003ca href=\"https:\/\/doi.org\/10.1038\/s43856-025-00841-7\" title=\"Shinoda, Chika et al. “N-glycan-modified α-L-iduronidase produced by transgenic silkworms ameliorates clinical signs in a Japanese macaque with mucopolysaccharidosis I.” Communications medicine vol. 5,1 128. 18 Apr. 2025, doi:10.1038\/s43856-025-00841-7\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1038\/s43856-025-00841-7\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSingh, Pragya et al. “Comparative Study of Placental Allografts with Distinct Layer Composition.” \u003ci\u003eInternational journal of molecular sciences\u003c\/i\u003e vol. 26,7 3406. 5 Apr. 2025, \u003ca rel=\"noopener\" title=\"Singh, Pragya et al. “Comparative Study of Placental Allografts with Distinct Layer Composition.” International journal of molecular sciences vol. 26,7 3406. 5 Apr. 2025, doi:10.3390\/ijms26073406\" href=\"https:\/\/doi.org\/10.3390\/ijms26073406\" target=\"_blank\"\u003edoi:10.3390\/ijms26073406\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eYang, Daniel et al. “Exosome-Seeded Cryogel Scaffolds for Extracellular Matrix Regeneration in the Repair of Articular Cartilage Defects: An In Vitro and In Vivo Rabbit Model Study.” \u003ci\u003ePolymers\u003c\/i\u003e vol. 17,7 975. 3 Apr. 2025, \u003ca rel=\"noopener\" title=\"Yang, Daniel et al. “Exosome-Seeded Cryogel Scaffolds for Extracellular Matrix Regeneration in the Repair of Articular Cartilage Defects: An In Vitro and In Vivo Rabbit Model Study.” Polymers vol. 17,7 975. 3 Apr. 2025, doi:10.3390\/polym17070975\" href=\"https:\/\/doi.org\/10.3390\/polym17070975\" target=\"_blank\"\u003edoi:10.3390\/polym17070975\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePrithiviraj, Sujeethkumar et al. “Compositional editing of extracellular matrices by CRISPR\/Cas9 engineering of human mesenchymal stem cell lines.” \u003ci\u003eeLife\u003c\/i\u003e vol. 13 RP96941. 28 Mar. 2025, \u003ca href=\"https:\/\/doi.org\/10.7554\/eLife.96941\" title=\"Prithiviraj, Sujeethkumar et al. “Compositional editing of extracellular matrices by CRISPR\/Cas9 engineering of human mesenchymal stem cell lines.” eLife vol. 13 RP96941. 28 Mar. 2025, doi:10.7554\/eLife.96941\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.7554\/eLife.96941\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMartino, Cameron et al. “SARS-CoV-2 infectivity can be modulated through bacterial grooming of the glycocalyx.” \u003ci\u003emBio\u003c\/i\u003e vol. 16,4 (2025): e0401524. \u003ca rel=\"noopener\" title=\"Martino, Cameron et al. “SARS-CoV-2 infectivity can be modulated through bacterial grooming of the glycocalyx.” mBio vol. 16,4 (2025): e0401524. doi:10.1128\/mbio.04015-24\" href=\"https:\/\/doi.org\/10.1128\/mbio.04015-24\" target=\"_blank\"\u003edoi:10.1128\/mbio.04015-24\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eGalvez, Paul et al. “In vitro and in vivo assessment of a new acellular human amnion\/chorion membrane device for guided bone regeneration.” \u003ci\u003eScientific reports\u003c\/i\u003e vol. 15,1 5483. 14 Feb. 2025, \u003ca href=\"https:\/\/doi.org\/10.1038\/s41598-025-88814-7\" title=\"Galvez, Paul et al. “In vitro and in vivo assessment of a new acellular human amnion\/chorion membrane device for guided bone regeneration.” Scientific reports vol. 15,1 5483. 14 Feb. 2025, doi:10.1038\/s41598-025-88814-7\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1038\/s41598-025-88814-7\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eLee, Hyesook et al. “Oral trehalose improves histological and behavior symptoms of mucopolysaccharidosis type II in iduronate 2-sulfatase deficient mice.” \u003c\/span\u003e\u003ci\u003eScientific reports\u003c\/i\u003e\u003cspan\u003e vol. 15,1 4882. 10 Feb. 2025, \u003ca rel=\"noopener\" title=\"Lee, Hyesook et al. “Oral trehalose improves histological and behavior symptoms of mucopolysaccharidosis type II in iduronate 2-sulfatase deficient mice.” Scientific reports vol. 15,1 4882. 10 Feb. 2025, doi:10.1038\/s41598-025-88362-0\" href=\"https:\/\/doi.org\/10.1038\/s41598-025-88362-0\" target=\"_blank\"\u003edoi:10.1038\/s41598-025-88362-0\u003c\/a\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eKopinski-Grünwald, Oliver et al. “Surface functionalization of microscaffolds produced by high-resolution 3D printing: A new layer of freedom.” \u003ci\u003eMaterials today. Bio\u003c\/i\u003e vol. 31 101452. 3 Jan. 2025, \u003ca href=\"https:\/\/doi.org\/10.1016\/j.mtbio.2025.101452\" title=\"Kopinski-Grünwald, Oliver et al. “Surface functionalization of microscaffolds produced by high-resolution 3D printing: A new layer of freedom.” Materials today. Bio vol. 31 101452. 3 Jan. 2025, doi:10.1016\/j.mtbio.2025.101452\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1016\/j.mtbio.2025.101452\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eHurt, Sarah C et al. “Antibodies to recombinant human alpha-L-iduronidase prevent disease correction in cortical bone in MPS I mice.” \u003ci\u003eMolecular therapy. Methods \u0026amp; clinical development\u003c\/i\u003e vol. 33,1 101405. 2 Jan. 2025, \u003ca rel=\"noopener\" title=\"Hurt, Sarah C et al. “Antibodies to recombinant human alpha-L-iduronidase prevent disease correction in cortical bone in MPS I mice.” Molecular therapy. Methods \u0026amp; clinical development vol. 33,1 101405. 2 Jan. 2025, doi:10.1016\/j.omtm.2024.101405\" href=\"https:\/\/doi.org\/10.1016\/j.omtm.2024.101405\" target=\"_blank\"\u003edoi:10.1016\/j.omtm.2024.101405\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003eThis product is intended for \u003cstrong\u003elaboratory research use only\u003c\/strong\u003e and is not for use in diagnostic procedures.\u003c\/p\u003e\n\u003ch2\u003eBackground\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eUnderstanding Glycosaminoglycans (GAGs) and Proteoglycans:\u003c\/strong\u003e\u003cbr\u003eGlycosaminoglycans (GAGs) are members of the polysaccharide family that play a crucial role in various biological processes. They consist of repeated disaccharide units, typically of hexosamine, (N-acetylated or N-sulfated), paired with uronic acid (GlcA or IdoA) or galactose. Many GAGs have an overall \"negative charge\" due to the addition of sulfate groups. This charge influences cell and extracellular matrix interactions.\u003c\/p\u003e\n\u003cp\u003eCommon examples of GAGs include Chondroitin Sulfate, Dermatan Sulfate, Heparin, Heparan Sulfate, and Keratan Sulfate.\u003c\/p\u003e\n\u003cp\u003eNOTE: Since Hyaluronic Acid is a non-sulfated GAG it cannot be detected by the Blyscan assay. If you need to measure hyaluronic acid, we recommend using the \u003ca href=\"https:\/\/ilexlife.com\/products\/biocolor-purple-jelley-hyaluronan-assay\" title=\"Biocolor Purple-Jelley™ Hyaluronic Acid Assay\" rel=\"noopener\" target=\"_blank\"\u003eBiocolor Purple-Jelley™ Hyaluronic Acid Assay\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe Role of Glycosaminoglycans in Tissues:\u003c\/strong\u003e\u003cbr\u003eGAGs and proteoglycans have essential functions in tissues and organisms including acting as a scaffold to provide biophysical support, and maintaining cartilage hydration. They also play a vital role in biochemical processes such as cell adhesion and signaling.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of Biocolor Ltd.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biocolor","offers":[{"title":"Standard Size Kit (110 assays) - B1000","offer_id":39975603568792,"sku":"B1000","price":640.0,"currency_code":"USD","in_stock":true},{"title":"Large Economy Size Kit (440 assays) - B3000","offer_id":39976203288728,"sku":"B3000","price":1735.0,"currency_code":"USD","in_stock":true},{"title":"Blyscan™ Dye Reagent (3 x 110 ml) - B1005","offer_id":40361005744280,"sku":"B1005","price":670.0,"currency_code":"USD","in_stock":true},{"title":"Blyscan™ Dye Dissociation Reagent (3 x 110 ml) - B1007","offer_id":40360919695512,"sku":"B1007","price":625.0,"currency_code":"USD","in_stock":true},{"title":"Blyscan™ Dye \u0026 Dye Dissociation Reagents (2 x 2 x 110 ml) - B1500","offer_id":40360966062232,"sku":"B1500","price":695.0,"currency_code":"USD","in_stock":true},{"title":"Blyscan™ sGAG Reference Standard (3 x 5 ml; 100 µg\/ml) - B1010","offer_id":40361020326040,"sku":"B1010","price":360.0,"currency_code":"USD","in_stock":true},{"title":"Blyscan™ sGAG Isolation \u0026 Concentration Pack (100 samples) - B1015","offer_id":43606354002132,"sku":"B1015","price":380.0,"currency_code":"USD","in_stock":true},{"title":"Green Assay Microtube (Refill Pack), 1.5 ml, 100 tubes - T1000","offer_id":47237036015828,"sku":"T1000","price":25.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/B1000kit.png?v=1706822193"},{"product_id":"biocolor-fastin-elastin-assay","title":"Biocolor Fastin™ Elastin Assay","description":"\u003cp\u003e\u003cspan style=\"color: #193925;\"\u003e\u003cstrong\u003eIntroducing the Fastin Assay Kit: Your Straightforward Solution for Elastin Quantification!\u003c\/strong\u003e Our user-friendly kit utilizes a dye-based method to measure elastin from \u003cem\u003ein-vivo\u003c\/em\u003e and \u003cem\u003ein-vitro\u003c\/em\u003e sources. It can be used to quantify various elastin forms, spanning from immature tropoelastin to mature, 'insoluble' elastin fibers.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #193925;\"\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"color: #193925;\"\u003eThe Biocolor Fastin™ Elastin Assay is a \u003cstrong\u003e\u003cem\u003equantitative\u003c\/em\u003e\u003c\/strong\u003e dye-binding method for the\u003cstrong\u003e\u003cem\u003e analysis of elastins\u003c\/em\u003e\u003c\/strong\u003e\u003cem\u003e \u003c\/em\u003ereleased into tissue culture medium and extracted from biological materials.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #193925;\"\u003eThe dye label employed is 5,10,15,20-tetraphenyl-21H,23H-porphine tetra-sulfonate (TPPS).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #193925;\"\u003eElastin forms that can be measured by the Fastin Assay as α-elastin:\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan style=\"color: #193925;\"\u003esoluble tropoelastins;\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #193925;\"\u003elathyrogenic elastins;\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #193925;\"\u003einsoluble elastins (following solubilization to elastin polypeptides [α-elastin, κ-elastin])\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cspan style=\"color: #193925;\"\u003eThe dye reagent binds to the ‘basic’ and ‘non-polar’ amino acid sequences found in mammalian elastins. For analysis of soluble elastin, samples should be free of any particulate material (cell debris, insoluble extracellular matrix material). The presence of other soluble proteins or of complex carbohydrates does not interfere with the Fastin Elastin Assay.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePrinciple of Method\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eStep 1.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eIncubation of samples containing soluble elastin with the Fastin Dye Reagent causes an elastin-dye complex to form. This insoluble complex then precipitates.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 2.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eDye-labelled elastin is then isolated by centrifugation and the unbound dye removed. Elastin-bound dye is then eluted and measured spectrophotometrically.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 3.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eThe elastin content of unknown samples can be calculated by comparison against a calibration curve prepared using a standard comprising water-soluble elastin (supplied with the kit).\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"Biocolor Fastin Elastin Assay Dye\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Fastin-elastin-Dye-Plate_480x480.jpg?v=1665086896\"\u003e\u003c\/p\u003e\n\u003ch2\u003eSuitable Samples\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eIn-vivo:\u003c\/em\u003e\u003c\/strong\u003e tissues and fluids. Insoluble elastin will first require conversion to water soluble α-elastin using the oxalic acid reagents and extraction protocol supplied with the kit. \u003cbr\u003e\u003cbr\u003e\u003cem\u003e\u003cstrong\u003eIn-vitro:\u003c\/strong\u003e\u003c\/em\u003e Elastin produced by cells during 2D\/3D cell culture. NB elastin in conditioned cell media is typically below the detection limit of the kit.\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eProduct Category:\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003ca aria-describedby=\"a11y-new-window-message\" title=\"Browse Extracellular Matrix Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eDetection Method:\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003eColorimetric Detection (513 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAssay Range:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 - 500 µg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLimit of Detection:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e50 µg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAssay Run-Time:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMeasurements Per Kit:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e110 in total (allows a maximum of 48 samples to be run in duplicate alongside a standard curve)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage (unopened kit):\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRoom Temperature (RT)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBiocolor Ltd.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCountry of Origin:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUnited Kingdom\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe standard size Fastin™ Elastin kit (cat. no. F2000) contains:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eDye Reagent (1x110ml)\u003c\/li\u003e\n\u003cli\u003eα-elastin Reference Standard (1x5ml, 1.0 mg\/ml soluble Bovine elastin)\u003c\/li\u003e\n\u003cli\u003eOxalic Acid (1x20ml)\u003c\/li\u003e\n\u003cli\u003ePrecipitating Reagent (1x30ml)\u003c\/li\u003e\n\u003cli\u003eDye Dissociation Reagent (1x30ml)\u003c\/li\u003e\n\u003cli\u003e1.5 ml micro-centrifuge tubes for dye-labelling reaction.\u003c\/li\u003e\n\u003cli\u003eAssay kit manual\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eOther Names\/Synonyms\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003esoluble tropoelastins, lathyrogenic elastins, Fastin Elastin\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Biocolor_Fastin_Elastin_Assay_Manual.pdf?v=1707153561\" title=\"Product Datasheet (PDS): Biocolor Fastin™ Elastin Assay Manual, Biocolor Fastin™ Elastin Assay Protocol\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Fastin-Elastin-Assay-kit-sds-en-eu-v10.pdf?v=1628271815\" title=\"Material Safety Data Sheet (MSDS) for Biocolor Fastin™ Elastin Assay Kit\" target=\"_blank\"\u003e\u003cspan\u003eMaterial Safety Data Sheet (MSDS)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Biocolor Fastin™ Elastin Assay - Frequently Asked Questions (FAQ)\" href=\"https:\/\/ilexlife.com\/pages\/biocolor-fastin-elastin-assay-frequently-asked-questions-faq\" target=\"_blank\"\u003e\u003cspan\u003eFrequently Asked Question (FAQ)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/biocolor-fastin-elastin-assay-references\" title=\"Biocolor Fastin™ Elastin Assay Kit Product References\" target=\"_blank\"\u003eProduct References (Extended)\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e2025 Product References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003eSong, Minwoo et al. “A Hierarchical Short Microneedle-Cupping Dual-Amplified Patch Enables Accelerated, Uniform, Pain-Free Transdermal Delivery of Extracellular Vesicles.” \u003ci\u003eNano-micro letters\u003c\/i\u003e vol. 18,1 11. 23 Jul. 2025, \u003ca rel=\"noopener\" title=\"Song, Minwoo et al. “A Hierarchical Short Microneedle-Cupping Dual-Amplified Patch Enables Accelerated, Uniform, Pain-Free Transdermal Delivery of Extracellular Vesicles.” Nano-micro letters vol. 18,1 11. 23 Jul. 2025, doi:10.1007\/s40820-025-01853-7\" href=\"https:\/\/doi.org\/10.1007\/s40820-025-01853-7\" target=\"_blank\"\u003edoi:10.1007\/s40820-025-01853-7\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eZhao, Wenshan et al. “Fibulin-1 deficiency alleviates liver fibrosis by inhibiting hepatic stellate cell activation via the p38 MAPK pathway.” \u003c\/span\u003e\u003ci\u003eCellular and molecular life sciences : CMLS\u003c\/i\u003e\u003cspan\u003e vol. 82,1 192. 5 May. 2025, \u003ca rel=\"noopener\" title=\"Zhao, Wenshan et al. “Fibulin-1 deficiency alleviates liver fibrosis by inhibiting hepatic stellate cell activation via the p38 MAPK pathway.” Cellular and molecular life sciences : CMLS vol. 82,1 192. 5 May. 2025, doi:10.1007\/s00018-025-05647-3\" href=\"https:\/\/doi.org\/10.1007\/s00018-025-05647-3\" target=\"_blank\"\u003edoi:10.1007\/s00018-025-05647-3\u003c\/a\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eHussein, Kamal H et al. “Efficacy of xenogeneic fresh and lyophilized amniotic membranes on the healing of experimentally induced full-thickness skin wounds in dogs.” \u003ci\u003eScientific reports\u003c\/i\u003e vol. 15,1 15605. 4 May. 2025, \u003ca href=\"https:\/\/doi.org\/10.1038\/s41598-025-95023-9\" title=\"Hussein, Kamal H et al. “Efficacy of xenogeneic fresh and lyophilized amniotic membranes on the healing of experimentally induced full-thickness skin wounds in dogs.” Scientific reports vol. 15,1 15605. 4 May. 2025, doi:10.1038\/s41598-025-95023-9\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1038\/s41598-025-95023-9\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSingh, Pragya et al. “Comparative Study of Placental Allografts with Distinct Layer Composition.” \u003ci\u003eInternational journal of molecular sciences\u003c\/i\u003e vol. 26,7 3406. 5 Apr. 2025, \u003ca rel=\"noopener\" title=\"Singh, Pragya et al. “Comparative Study of Placental Allografts with Distinct Layer Composition.” International journal of molecular sciences vol. 26,7 3406. 5 Apr. 2025, doi:10.3390\/ijms26073406\" href=\"https:\/\/doi.org\/10.3390\/ijms26073406\" target=\"_blank\"\u003edoi:10.3390\/ijms26073406\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMcCarthy, Michelle E et al. “Decellularization of Human Digits: A Step Towards Off-the-Shelf Composite Allograft Transplantation.” \u003ci\u003eBioengineering (Basel, Switzerland)\u003c\/i\u003e vol. 12,4 383. 3 Apr. 2025, \u003ca href=\"https:\/\/doi.org\/10.3390\/bioengineering12040383\" title=\"McCarthy, Michelle E et al. “Decellularization of Human Digits: A Step Towards Off-the-Shelf Composite Allograft Transplantation.” Bioengineering (Basel, Switzerland) vol. 12,4 383. 3 Apr. 2025, doi:10.3390\/bioengineering12040383\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.3390\/bioengineering12040383\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eDe Miguel-Gómez, Lucía et al. “Toward human uterus tissue engineering: Uterine decellularization in a non-human primate species.” \u003ci\u003eActa obstetricia et gynecologica Scandinavica\u003c\/i\u003e vol. 104,3 (2025): 483-493. \u003ca rel=\"noopener\" title=\"De Miguel-Gómez, Lucía et al. “Toward human uterus tissue engineering: Uterine decellularization in a non-human primate species.” Acta obstetricia et gynecologica Scandinavica vol. 104,3 (2025): 483-493. doi:10.1111\/aogs.15030\" href=\"https:\/\/doi.org\/10.1111\/aogs.15030\" target=\"_blank\"\u003edoi:10.1111\/aogs.15030\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eCosta, E et al. “Role of Myofibroblasts in the Repair of Iatrogenic Preterm Membranes Subjected to Mechanical Stimulation.” \u003ci\u003ePrenatal diagnosis\u003c\/i\u003e vol. 45,1 (2025): 102-112. \u003ca href=\"https:\/\/doi.org\/10.1002\/pd.6722\" title=\"Costa, E et al. “Role of Myofibroblasts in the Repair of Iatrogenic Preterm Membranes Subjected to Mechanical Stimulation.” Prenatal diagnosis vol. 45,1 (2025): 102-112. doi:10.1002\/pd.6722\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1002\/pd.6722\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003eThis product is intended for \u003cstrong\u003elaboratory research use only\u003c\/strong\u003e and is not for use in diagnostic procedures.\u003c\/p\u003e\n\u003ch2\u003eBackground\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eUnderstanding Elastin: The Key to Tissue Flexibility:\u003c\/strong\u003e\u003cbr\u003eTissues such as lungs, heart and blood vessels need to be able to stretch and recoil repeatedly throughout an organism's life. The protein Elastin is largely responsible for this elasticity. Elastin is initially secreted by elastin-producing cells as a soluble monomeric precursor known as tropoelastin. This undergoes a process of self-assembly and cross-linking with other extracellular matrix components that results in the formation of \"insoluble\" elastin fibers.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of Biocolor products.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biocolor","offers":[{"title":"Standard Size Kit (110 assays) - F2000","offer_id":39993301336216,"sku":"F2000","price":705.0,"currency_code":"USD","in_stock":true},{"title":"Large Economy Size Kit (440 assays) - F4000","offer_id":39993301368984,"sku":"F4000","price":2010.0,"currency_code":"USD","in_stock":true},{"title":"Fastin™ Dye Reagent (3 x 110 ml) - F2300","offer_id":40931056681112,"sku":"F2300","price":1385.0,"currency_code":"USD","in_stock":true},{"title":"Fastin™ Dissociation Reagent (3 x 30 ml) - FDISS","offer_id":40931127853208,"sku":"FDISS","price":295.0,"currency_code":"USD","in_stock":true},{"title":"Fastin™ Elastin Reference Standard (1 mg\/ml; 3 x 5 ml) - F2010","offer_id":40931096690840,"sku":"F2010","price":478.0,"currency_code":"USD","in_stock":true},{"title":"Green Assay Microtube (Refill Pack), 1.5 ml, 100 tubes - T1000","offer_id":47237038276820,"sku":"T1000","price":25.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/F2000Kit.png?v=1706813506"},{"product_id":"biocolor-purple-jelley-hyaluronan-assay","title":"Biocolor Purple-Jelley™ Hyaluronan \/ Hyaluronic Acid Assay","description":"\u003cp\u003e\u003cspan style=\"color: #193925;\"\u003e\u003cspan\u003e\u003cstrong\u003eBiocolor’s Purple-Jelley Assay Kit is the perfect tool for accurate measurement of hyaluronic acid \/ hyaluronan levels in your samples.\u003c\/strong\u003e This colorimetric assay is optimized for quantitative analysis of \u003cem\u003ein-vivo\u003c\/em\u003e, tissue-derived hyaluronic acid and includes full step-by-step instructions.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan style=\"color: #193925;\"\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Biocolor \u003cstrong\u003ePurple-Jelley Hyaluronic Acid Assay\u003c\/strong\u003e is a quantitative, dye-based method for measurement of hyaluronic acid\/hyaluronate\/hyaluronan derived from tissues.\u003c\/p\u003e\n\u003cp\u003eThe assay manual includes methods for the user to remove tissue protein and sulfated glycosaminoglycans before measuring the isolated hyaluronic acid (HA).\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003ePrinciple of Method\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eDuring the assay, hyaluronic acid is selectively purified during the assay sample preparation protocol. This is then reacted with the Purple-Jelley dye reagent (\u003cspan\u003econtaining ‘Stains-all’ dye)\u003c\/span\u003e, and the absorption of the characteristic third wavelength recorded. By comparison with a calibration curve the hyaluronic acid content of the sample can be measured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 1.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eThe assay protocol takes tissue samples through a sequential sample preparation protocol which involves enzymatic protein digestion, followed by precipitation and purification of GAGs, culminating in the precipitation of purified Hyaluronic acid.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep2.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eThe processed sample is then incubated for 10 minutes with the Purple-Jelley dye reagent, forming a colored product which can be measured spectrophotometrically.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 3.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eThe Hyaluronic acid content of unknown samples can be calculated by comparison against a calibration curve prepared using a standard comprising hyaluronic acid (supplied with the kit).\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"Biocolor Purple Jelley Hyaluronic Acid Assay Dye\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Hyaluronic-acid-Dye-Plate_480x480.jpg?v=1665087125\"\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch2\u003eSample Type\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eTissue extracts.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRemoval of tissue protein before assay is essential in order to isolate the HA from potentially dye binding proteins; and sulfated glycosaminoglycans need to be removed before measuring the isolated HA, from a two step critical electrolyte salting out process (CEC). Methods for protein removal and sGAG removal can be found in the product manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/h2\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Category:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003ca aria-describedby=\"a11y-new-window-message\" title=\"Browse Extracellular Matrix Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px;\"\u003e\u003cstrong\u003eDetection Method:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px;\"\u003e\u003cspan\u003eColorimetric Detection (655 nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px;\"\u003e\u003cstrong\u003eAssay Range:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px;\"\u003e0 - 100 µg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px;\"\u003e\u003cstrong\u003eLimit of Detection:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px;\"\u003e10 µg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px;\"\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px;\"\u003e0.2 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px;\"\u003e\u003cstrong\u003eMeasurements Per Kit:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px;\"\u003e\u003cspan\u003e100 in total (allows a maximum of 46 samples to be run in duplicate alongside a standard curve)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage (unopened kit):\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRoom Temperature (RT)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eBiocolor Ltd.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCountry of Origin:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eUnited Kingdom\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e\u003cspan\u003eKit Contents\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe standard size Purple-Jelley™ Hyaluronic Acid kit (cat. no. H1000) contains:\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003ePurple-Jelley Dye Reagent (1x 20 ml)\u003c\/li\u003e\n\u003cli\u003eHyaluronan Reference Standard (1x 5 ml, 0.2 mg\/ml soluble Hyaluronic Acid)\u003c\/li\u003e\n\u003cli\u003ePrecipitating Reagent (2x 34 ml)\u003c\/li\u003e\n\u003cli\u003eSodium Chloride (1x 20 ml)\u003c\/li\u003e\n\u003cli\u003eCetylpyridinium Chloride (1x 20 ml)\u003c\/li\u003e\n\u003cli\u003eTRIS-buffered Saline (5x tablets)\u003c\/li\u003e\n\u003cli\u003e2 ml screw-cap tubes for preparation of samples\u003c\/li\u003e\n\u003cli\u003eAssay kit manual\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eExamples of Results\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe distribution of HA in tissues as produced in this assay procedure is listed in \u003cem\u003eTable 1 \u003c\/em\u003eand is given only as an outline guide. In adult animals the HA percentage gradually increases during ageing as muscle and fat mass decreases.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"Typical distribution of hyaluronic acid (HA) in various mammalian tissues\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/distributionofhyaluronicacidintissue.png?v=1672714142\"\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003eOther Names\/Synonyms\u003c\/h2\u003e\n\u003cp\u003eHyaluronic acid, Hyaluronan, HA, Purple-Jelly Hyaluronan Assay\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Biocolor_Purple-Jelley_Hyaluronic_Acid_Assay_Manual.pdf?v=1707153233\" title=\"Product Datasheet (PDS): Purple-Jelley Hyaluronan Assay protocol, Purple-Jelley Hyaluronan Assay manual\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Purple-Jelley-Hyaluronan-Assay-H1000-sds-en-eu-v4.pdf?v=1628270889\" title=\"Material Safety Data Sheet (MSDS) for Biocolor Purple-Jelley Hyaluronan Assay kit\" target=\"_blank\"\u003e\u003cspan\u003eMaterial Safety Data Sheet (MSDS)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/biocolor-purple-jelley-hyaluronic-acid-assay-frequently-asked-questions-faq\" title=\"Biocolor Purple-Jelley™ Hyaluronic Acid Assay - Frequently Asked Questions (FAQ)\" target=\"_blank\"\u003e\u003cspan\u003eFrequently Asked Questions (FAQ)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca title=\"Published Biocolor Purple-Jelley Hyaluronan Assay References\" href=\"https:\/\/ilexlife.com\/pages\/biocolor-purple-jelley-hyaluronic-acid-assay-references\" target=\"_blank\"\u003eProduct References (Extended)\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e2025 Product References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eSingh, Pragya et al. “Comparative Study of Placental Allografts with Distinct Layer Composition.” \u003ci\u003eInternational journal of molecular sciences\u003c\/i\u003e vol. 26,7 3406. 5 Apr. 2025, \u003ca rel=\"noopener\" title=\"Singh, Pragya et al. “Comparative Study of Placental Allografts with Distinct Layer Composition.” International journal of molecular sciences vol. 26,7 3406. 5 Apr. 2025, doi:10.3390\/ijms26073406\" href=\"https:\/\/doi.org\/10.3390\/ijms26073406\" target=\"_blank\"\u003edoi:10.3390\/ijms26073406\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eIkawa, Tetsuya et al. “Impact of Hyaluronic Acid on the Cutaneous T-Cell Lymphoma Microenvironment: A Novel Anti-Tumor Mechanism of Bexarotene.” \u003c\/span\u003e\u003ci\u003eCancers\u003c\/i\u003e\u003cspan\u003e vol. 17,2 324. 20 Jan. 2025, \u003ca href=\"https:\/\/doi.org\/10.3390\/cancers17020324\" title=\"Ikawa, Tetsuya et al. “Impact of Hyaluronic Acid on the Cutaneous T-Cell Lymphoma Microenvironment: A Novel Anti-Tumor Mechanism of Bexarotene.” Cancers vol. 17,2 324. 20 Jan. 2025, doi:10.3390\/cancers17020324\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.3390\/cancers17020324\u003c\/a\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003eThis product is intended for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003elaboratory research use only\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand is not for use in diagnostic procedures.\u003c\/p\u003e\n\u003ch2\u003eRelated Products\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" title=\"Browse Extracellular Matrix Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Recombinant Proteinase K\" href=\"https:\/\/ilexlife.com\/products\/recombinant-proteinase-k\" target=\"_blank\"\u003eProteinase K\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/3-d-life-pva-ha-hydrogel-kit\" title=\"3-D Life PVA-HA Hydrogel Kit Product Page\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e PVA-HA Hydrogel Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Dextran-HA Hydrogel Kit product page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-dextran-ha-hydrogel-kit\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Dextran-HA Hydrogel Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/products\/hyaluronidase-bovine-testes\" title=\"Hyaluronidase Enzyme (Bovine purified) product page\" target=\"_blank\"\u003eHyaluronidase Enzyme (Bovine purified)\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBackground\u003c\/h2\u003e\n\u003cp\u003eHyaluronic acid, in its hydrated form, is a unique polysaccharide polymer, often referred to as a '\u003cem\u003egentle giant\u003c\/em\u003e'. It is found in many tissues, but particularly in cartilage and synovial fluid and consists of a lengthy, flexible, non-branching chain with a repeating disaccharide pattern. This pattern comprises alternating uronic acid and aminosugar units. Hyaluronic acid  is involved in a wide range of roles including tissue hydration and lubrication, cell signaling, extracellular matrix reorganization and wound repair.\u003c\/p\u003e\n\u003cp\u003eThis involvement of Hyaluronic acid in tissue lubrication and wound healing has meant it has become a well-known additive to both medical and cosmetic skincare treatments, as well as treatments for osteoarthritis. The ability to assay or measure hyaluronic acid is therefore of interest to scientific researchers from a range of disciplines.\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of Biocolor products.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biocolor","offers":[{"title":"Standard Size Kit (100 assays) - H1000","offer_id":39993360646296,"sku":"H1000","price":645.0,"currency_code":"USD","in_stock":true},{"title":"Large Economy Size Kit (400 assays) - H2000","offer_id":39993360679064,"sku":"H2000","price":1735.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/H1000Kit.png?v=1706819629"},{"product_id":"biocolor-sircol-insoluble-collagen-assay","title":"Biocolor Sircol™ Insoluble Collagen Assay","description":"\u003cp\u003e\u003cspan\u003eAs collagens age, they become increasingly crosslinked and insoluble - characteristics necessary for the structural role they play in tissues such as bone. Biocolor's Sircol™ INSOLUBLE Collagen Kit is an easy-to-use dye-binding assay that can be used to accurately measure the amount of insoluble collagen content in both \u003cem\u003ein-vivo\u003c\/em\u003e and \u003cem\u003ein-vitro\u003c\/em\u003e tissues.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003eBiocolor Sircol™ INSOLUBLE Collagen Assay Kit\u003c\/strong\u003e is a dye-binding assay designed for accurate quantification and measurement of insoluble or cross-linked collagen fibers. It is ideal for analyzing materials from sources such as tissues, bone, and calcified tissue.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThe assay can be used to assess the rate of production of newly laid down collagen fibres during periods of rapid growth, development, tissue repair, remodeling and wound healing.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eIt replaces the conventional hydroxyproline analysis. \u003cstrong\u003eNo more working with concentrated acids or alkalis!\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli\u003eThe Sircol Insoluble Assay uses mild acid and temperature treatment for 2 to 3 hours.\u003c\/li\u003e\n\u003cli\u003eThe solubility conversion to denatured collagen and subsequent measurement can be completed within one working day.\u003c\/li\u003e\n\u003cli\u003eThe ratio of soluble and insoluble collagens can be measured using the same Sircol Dye Reagent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePrinciple of Method\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eSircol™ Dye Reagent contains Sirius Red - a linear anionic dye with sulphonic acid side chain groups. Under assay conditions the Sircol dye binds the basic groups of soluble collagen molecules. Maximal binding occurs in collagens possessing intact triple helix organization as the highly ordered Gly-X-Yn helical structure of tropocollagen further contributes to dye binding. This results in a high degree of dye-collagen specificity. Affinity is progressively reduced during heat denaturation 4ºC due to the unwinding of the triple helix and formation of random chains.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 1.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eSamples being assayed for insoluble collagen must first undergo a 2-3 hour pre-treatment with Sircol Fragmentation reagent. This converts insoluble collagen into water-soluble gelatin can then be assayed.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eAddition of Sircol Dye Reagent to these pre-treated insoluble collagen samples results in the formation of a denatured collagen-dye complex. This complex then precipitates during the dye incubation period and is subsequently isolated by centrifugation, followed by washing to remove unbound dye. The Denatured collagen-bound dye is then  eluted and measured spectrophotometrically.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 3.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eThe insoluble collagen content of unknown samples is quantified by comparison against a calibration curve prepared using a the denatured collagen standard supplied with the kit.\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Sircol-Insoluble-Collagen-Dye-Plate_480x480.jpg?v=1665085859\" alt=\"Biocolor Sircol Insoluble Collagen Assay Sirius Red Dye\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Category:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e\u003ca aria-describedby=\"a11y-new-window-message\" title=\"Browse Extracellular Matrix Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Method:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eColorimetric Detection (556 nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAssay Range:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 - 1000 µg\/ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLimit of Detection:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100 µg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAssay Run-Time:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 hours (including conversion of native insoluble collagen to soluble denatured collagen)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eType of Collagen Measured:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCovalent crosslinked insoluble collagen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMeasurements Per Kit:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e110 in total (allows a maximum of 46 samples to be run in duplicate alongside a standard curve).\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage (unopened kit):\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRoom Temperature (RT)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eBiocolor Ltd.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCountry of Origin:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eUnited Kingdom\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"scrollable-wrapper\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch2\u003eKit Contents\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe standard size Sircol™ Insoluble Collagen Assay kit (cat. no. S2000) contains:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSircol Dye Reagent (1x 110 ml)\u003c\/li\u003e\n\u003cli\u003eDenatured Collagen Reference Standard (1x 5 ml, 1.0 mg\/ml)\u003c\/li\u003e\n\u003cli\u003eAcid-Salt Wash Reagent (1x 20 ml)\u003c\/li\u003e\n\u003cli\u003eFragmentation Reagent (1x 110 ml)\u003c\/li\u003e\n\u003cli\u003eAlkali Reagent (1x 110 ml)\u003c\/li\u003e\n\u003cli\u003e2 ml screw-cap tubes for preparation of samples.\u003c\/li\u003e\n\u003cli\u003eAssay kit manual\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eApplication Notes\u003c\/h2\u003e\n\u003cp\u003eInsoluble collagens must be converted into soluble form prior to assay. Instructions and reagents are provided with the kit. Depending on the sample, this will require prior salt\/acid\/acid-pepsin extraction.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eNon-mammalian collagens may result in a reduced limit of detection. We recommend use of an assay standard matched to the non-mammalian species under assay.\u003c\/p\u003e\n\u003ch2\u003eWhich Sircol Collagen Assay should I use for my application?\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/SIRCOL-flow_insoluble_soluble.png?v=1621167801\" alt=\"Which Sircol Collagen Assay should I use for my application? flow chart\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eResources\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener noreferrer\" title=\"Product Datasheet (PDS): Biocolor Sircol™ Insoluble Collagen Assay Manual and Protocol\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Biocolor_Sircol_Insoluble_Collagen_Assay_Manual.pdf?v=1707153923\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"MSDS for Sircol INSOLUBLE Collagen Assay Kit\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Sircol-Insoluble-Collagen-Assay-Kit-sds-en-eu-v4.pdf?v=1628263918\" target=\"_blank\"\u003e\u003cspan\u003eMaterial Safety Data Sheet (MSDS)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Biocolor Sircol™ Insoluble Collagen Assay - Frequently Asked Questions (FAQ)\" href=\"https:\/\/ilexlife.com\/pages\/biocolor-sircol-insoluble-collagen-assay-frequently-asked-questions-faq\" target=\"_blank\"\u003eFrequently Asked Questions (FAQ)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e2024-2025 Product References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003eWen, Liru et al. “Establishment of an ex vivo cartilage damage model by combined collagenase treatment and mechanical loading.” \u003c\/span\u003e\u003ci\u003eArthritis research \u0026amp; therapy\u003c\/i\u003e\u003cspan\u003e vol. 27,1 30. 11 Feb. 2025, \u003ca rel=\"noopener\" title=\"Wen, Liru et al. “Establishment of an ex vivo cartilage damage model by combined collagenase treatment and mechanical loading.” Arthritis research \u0026amp; therapy vol. 27,1 30. 11 Feb. 2025, doi:10.1186\/s13075-025-03499-7\" href=\"https:\/\/doi.org\/10.1186\/s13075-025-03499-7\" target=\"_blank\"\u003edoi:10.1186\/s13075-025-03499-7\u003c\/a\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eDagnachew, Yinebeb Mezgebu et al. “Collagen deposition in lung parenchyma driven by depletion of interstitial Lyve-1\u003csup\u003e+\u003c\/sup\u003e macrophages prevents cigarette smoke-induced emphysema and loss of airway function.” \u003ci\u003eFrontiers in immunology\u003c\/i\u003e vol. 15 1493395. 3 Jan. 2025, \u003ca href=\"https:\/\/doi.org\/10.3389\/fimmu.2024.1493395\" title=\"Dagnachew, Yinebeb Mezgebu et al. “Collagen deposition in lung parenchyma driven by depletion of interstitial Lyve-1+ macrophages prevents cigarette smoke-induced emphysema and loss of airway function.” Frontiers in immunology vol. 15 1493395. 3 Jan. 2025, doi:10.3389\/fimmu.2024.1493395\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.3389\/fimmu.2024.1493395\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eDe Miguel-Gómez, Lucía et al. “Toward human uterus tissue engineering: Uterine decellularization in a non-human primate species.” \u003ci\u003eActa obstetricia et gynecologica Scandinavica\u003c\/i\u003e vol. 104,3 (2025): 483-493. \u003ca href=\"https:\/\/doi.org\/10.1111\/aogs.15030\" title=\"De Miguel-Gómez, Lucía et al. “Toward human uterus tissue engineering: Uterine decellularization in a non-human primate species.” Acta obstetricia et gynecologica Scandinavica vol. 104,3 (2025): 483-493. doi:10.1111\/aogs.15030\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1111\/aogs.15030\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eChoi, Soon Jin et al. “Increased efficiency of peripheral nerve regeneration using supercritical carbon dioxide-based decellularization in acellular nerve graft.” \u003ci\u003eScientific reports\u003c\/i\u003e vol. 14,1 23696. 10 Oct. 2024, \u003ca rel=\"noopener\" title=\"Choi, Soon Jin et al. “Increased efficiency of peripheral nerve regeneration using supercritical carbon dioxide-based decellularization in acellular nerve graft.” Scientific reports vol. 14,1 23696. 10 Oct. 2024, doi:10.1038\/s41598-024-72672-w\" href=\"https:\/\/doi.org\/10.1038\/s41598-024-72672-w\" target=\"_blank\"\u003edoi:10.1038\/s41598-024-72672-w\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMendibil, Unai et al. “Development of bioactive solid-foam scaffolds from decellularized cartilage with chondrogenic and osteogenic properties.” \u003ci\u003eMaterials today. Bio\u003c\/i\u003e vol. 28 101228. 3 Sep. 2024, \u003ca href=\"https:\/\/doi.org\/10.1016\/j.mtbio.2024.101228\" title=\"Mendibil, Unai et al. “Development of bioactive solid-foam scaffolds from decellularized cartilage with chondrogenic and osteogenic properties.” Materials today. Bio vol. 28 101228. 3 Sep. 2024, doi:10.1016\/j.mtbio.2024.101228\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1016\/j.mtbio.2024.101228\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eKelly-Scumpia, Kindra M et al. “Modulating the extracellular matrix to treat wound healing defects in Ehlers-Danlos syndrome.” \u003ci\u003eiScience\u003c\/i\u003e vol. 27,9 110676. 6 Aug. 2024, \u003ca rel=\"noopener\" title=\"Kelly-Scumpia, Kindra M et al. “Modulating the extracellular matrix to treat wound healing defects in Ehlers-Danlos syndrome.” iScience vol. 27,9 110676. 6 Aug. 2024, doi:10.1016\/j.isci.2024.110676\" href=\"https:\/\/doi.org\/10.1016\/j.isci.2024.110676\" target=\"_blank\"\u003edoi:10.1016\/j.isci.2024.110676\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eVikranth, Thirapurasundari et al. “Decellularisation and Characterisation of Porcine Pleura as Bioscaffolds in Tissue Engineering.” \u003ci\u003eJournal of tissue engineering and regenerative medicine\u003c\/i\u003e vol. 2024 9940673. 8 Jul. 2024, \u003ca href=\"https:\/\/doi.org\/10.1155\/2024\/9940673\" title=\"Vikranth, Thirapurasundari et al. “Decellularisation and Characterisation of Porcine Pleura as Bioscaffolds in Tissue Engineering.” Journal of tissue engineering and regenerative medicine vol. 2024 9940673. 8 Jul. 2024, doi:10.1155\/2024\/9940673\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1155\/2024\/9940673\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eChen, Te-An et al. “Engineering a robust and anisotropic cardiac-specific extracellular matrix scaffold for cardiac patch tissue engineering.” \u003ci\u003eMatrix biology plus\u003c\/i\u003e vol. 23 100151. 25 May. 2024, \u003ca rel=\"noopener\" title=\"Chen, Te-An et al. “Engineering a robust and anisotropic cardiac-specific extracellular matrix scaffold for cardiac patch tissue engineering.” Matrix biology plus vol. 23 100151. 25 May. 2024, doi:10.1016\/j.mbplus.2024.100151\" href=\"https:\/\/doi.org\/10.1016\/j.mbplus.2024.100151\" target=\"_blank\"\u003edoi:10.1016\/j.mbplus.2024.100151\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eHarmon, Katrina A et al. “Varying Properties of Extracellular Matrix Grafts Impact Their Durability and Cell Attachment and Proliferation in an \u003ci\u003eIn Vitro\u003c\/i\u003e Chronic Wound Model.” \u003ci\u003eJournal of tissue engineering and regenerative medicine\u003c\/i\u003e vol. 2024 6632276. 26 Apr. 2024, \u003ca href=\"https:\/\/doi.org\/10.1155\/2024\/6632276\" title=\"Harmon, Katrina A et al. “Varying Properties of Extracellular Matrix Grafts Impact Their Durability and Cell Attachment and Proliferation in an In Vitro Chronic Wound Model.” Journal of tissue engineering and regenerative medicine vol. 2024 6632276. 26 Apr. 2024, doi:10.1155\/2024\/6632276\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1155\/2024\/6632276\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMartin, Meghan et al. “Differential Development of the Chordae Tendineae and Anterior Leaflet of the Bovine Mitral Valve.” \u003ci\u003eJournal of cardiovascular development and disease\u003c\/i\u003e vol. 11,4 106. 29 Mar. 2024, \u003ca rel=\"noopener\" title=\"Martin, Meghan et al. “Differential Development of the Chordae Tendineae and Anterior Leaflet of the Bovine Mitral Valve.” Journal of cardiovascular development and disease vol. 11,4 106. 29 Mar. 2024, doi:10.3390\/jcdd11040106\" href=\"https:\/\/doi.org\/10.3390\/jcdd11040106\" target=\"_blank\"\u003edoi:10.3390\/jcdd11040106\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003eThis product is intended for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003elaboratory research use only\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand is not for use in diagnostic procedures.\u003c\/p\u003e\n\u003ch2\u003eRelated Products\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/biocolor-sircol-2-0-soluble-collagen-assay\" title=\"Biocolor Sircol™ 2.0 Soluble Collagen Assay (96-well plate format)\" rel=\"noopener\" target=\"_blank\"\u003eBiocolor Sircol™ 2.0 Soluble Collagen Assay (96-well plate format)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Biocolor Sircol™ Soluble Collagen Assay product page\" href=\"https:\/\/ilexlife.com\/products\/biocolor-sircol-soluble-collagen-assay\" target=\"_blank\"\u003eBiocolor Sircol™ Soluble Collagen Assay (original version)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Collagen and Hydroxyproline Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/assays-for-collagen-analysis\" rel=\"null\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eCollagen and Hydroxyproline Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" title=\"Browse Extracellular Matrix Assays product collection\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" title=\"Extracellular Matrix Molecules product collection\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Promed Bioscience high-purity collagen biomaterials\" href=\"https:\/\/ilexlife.com\/collections\/promed-bioscience-collagen\" target=\"_blank\"\u003ePromed Bioscience high-purity collagen biomaterials\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of Biocolor products.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biocolor","offers":[{"title":"Standard Size Kit (110 assays), Bovine Standard - S2000","offer_id":39993582780568,"sku":"S2000","price":599.0,"currency_code":"USD","in_stock":true},{"title":"Large Economy Size Kit (440 assays) - S6000","offer_id":39993582813336,"sku":"S6000","price":1695.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Insoluble\/Soluble Collagen Assays Combination Pack (1X S1000 + 1X S2000) - S4000","offer_id":39993763168408,"sku":"S4000","price":1120.0,"currency_code":"USD","in_stock":true},{"title":"S2000 Denatured Collagen Standard 1 mg\/ml, (3 x 5 ml) - S2010","offer_id":40590580056216,"sku":"S2010","price":358.0,"currency_code":"USD","in_stock":true},{"title":"S2000 Fragmentation Reagent, (3 x 110 ml) - SFRAG","offer_id":40590581956760,"sku":"SFRAG","price":415.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Dye Reagent (3 x 110 ml) - S1005","offer_id":40596666253464,"sku":"S1005","price":762.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Dye Release Reagent (3 x 110 ml) - S1007","offer_id":40596684505240,"sku":"S1007","price":355.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Dye \u0026 Dye Release Reagents (2 x 110 ml, 2 x 110 ml) - S1500","offer_id":43619807166676,"sku":"S1500","price":785.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Acid-Salt Wash (3 x 20 ml) - SWASH","offer_id":40596671398040,"sku":"SWASH","price":311.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/S2000kit.png?v=1706899480"},{"product_id":"biocolor-sircol-soluble-collagen-assay","title":"Biocolor Sircol™ Soluble Collagen Assay (Original Version)","description":"\u003cp\u003e\u003cstrong\u003eNOTE: The new \u003ca title=\"Biocolor Sircol™ 2.0 Soluble Collagen Assay Product Page\" href=\"https:\/\/ilexlife.com\/products\/biocolor-sircol-2-0-soluble-collagen-assay\" target=\"_blank\"\u003eBiocolor Sircol™ 2.0 Soluble Collagen Assay\u003c\/a\u003e is now available!\u003c\/strong\u003e This latest addition to the highly cited family of \"Sircol\" collagen kits combines an\u003cbr\u003e\u003cem\u003eupdated 96-well microplate format\u003c\/em\u003e with an \u003cem\u003eimproved dye formulation\u003c\/em\u003e to significantly enhance collagen sensitivity and specificity. This makes it possible to directly analyze collagen from complex samples such as conditioned media during 2D or 3D cell culture.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eWe recommend that new customers use our Sircol 2.0 assay kit as it improves sensitivity, specificity, and user-friendliness; however, we will continue to offer the older, \"original\" kit as well.\u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe Biocolor \u003c\/span\u003e\u003cstrong\u003eSircol\u003c\/strong\u003e\u003cstrong\u003e™ Soluble Collagen Assa\u003c\/strong\u003e\u003cstrong\u003ey\u003c\/strong\u003e\u003cspan\u003e is a \u003c\/span\u003e\u003cstrong\u003e\u003cem\u003equantitative\u003c\/em\u003e\u003c\/strong\u003e\u003cspan\u003e dye-binding assay for \u003c\/span\u003e\u003cem\u003e\u003cstrong\u003emeasurement of both acid-soluble and pepsin-soluble collagens\u003c\/strong\u003e\u003c\/em\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe assay can assess the rate of newly synthesized collagen. New collagen is produced during periods of rapid growth and development. New collagen is also generated during inflammation, wound healing and tumor development.\u003c\/p\u003e\n\u003cp\u003eThe Sircol Assay can monitor collagen produced \u003cem\u003ein situ,\u003c\/em\u003e or during \u003cem\u003ein-vitro\u003c\/em\u003e cell culture. It can also measure \u003cem\u003ein-vitro\u003c\/em\u003e extracellular matrix formation.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eThis assay is not suitable for covalent cross-linked collagen. However, the \u003ca href=\"https:\/\/ilexlife.com\/products\/biocolor-sircol-insoluble-collagen-assay\" title=\"Biocolor Sircol Insoluble Collagen Assay\" target=\"_blank\"\u003eSircol Insoluble Collagen Assay\u003c\/a\u003e (S2000) replaces hydroxyproline analysis. This allows the customer to measure insoluble collagen within one working day.\u003c\/em\u003e\u003c\/p\u003e\n\u003ch2\u003ePrinciple of Method\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eSircol™ Dye Reagent contains Sirius Red - a linear anionic dye with sulphonic acid side chain groups. Under assay conditions the Sircol dye binds the basic groups of soluble collagen molecules. Maximal binding occurs in collagens possessing intact triple helix organization as the highly ordered Gly-X-Yn helical structure of tropocollagen further contributes to dye binding. This results in a high degree of dye-collagen specificity. Affinity is progressively reduced during heat denaturation 4ºC due to the unwinding of the triple helix and formation of random chains.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eAddition of Sircol Dye Reagent to samples containing soluble collagen results in the formation of a collagen-dye complex. This complex then precipitates during the dye incubation period and is subsequently isolated by centrifugation, followed by washing to remove unbound dye.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eCollagen-bound dye is then eluted and measured spectrophotometrically.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStep 3.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eThe collagen content of unknown samples is quantified by comparison against a calibration curve prepared using a collagen standard supplied with the kit.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"Biocolor Sircol Soluble Collagen Assay Sirius Red Dye\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Sircol-Insoluble-Collagen-Dye-Plate_480x480.jpg?v=1665085859\"\u003e\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Category:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" title=\"Browse Extracellular Matrix Assays product collection\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Method:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eColorimetric Detection (556 nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAssay Range:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 - 150 µg\/ml or 0 - 500 µg\/ml (depending on setup)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLimit of Detection:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10 µg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAssay Run-Time:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1.5 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eThe sensitivity of the Sircol Soluble Collagen Assay is 1.0 µg collagen. Quantities as low as 1.0 µg of collagen can be recovered from a 1 ml volume if the Collagen Isolation \u0026amp; Concentration protocol is used (See Sircol assay manual for more detail).\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eType of Collagen Measured:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eAcid-soluble and pepsin-soluble collagens.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSuitable for all mammalian collagens, Types I to V.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMeasurements Per Kit:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e110 in total (allows a maximum of 48 samples to be run in duplicate alongside a standard curve).\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage (unopened kit):\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRoom Temperature (RT)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eBiocolor Ltd.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCountry of Origin:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eUnited Kingdom\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSuitable Samples\u003c\/h2\u003e\n\u003cp\u003eSoluble* collagens from mammalian sources**:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cem\u003eIn-vivo: \u003c\/em\u003etissues, cartilages, fluids.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cem\u003eIn-vitro:\u003c\/em\u003e 2D\/3D culture extracellular matrices. \u003cspan\u003eCustomers wishing to analyze conditioned media should first consider the new Sircol 2.0 assay kit.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003e* Collagens must be in soluble form, depending on sample this will require prior salt\/acid\/acid-pepsin extraction.\u003c\/em\u003e\u003c\/p\u003e\n\u003cdiv id=\"w-node-_1b2e4afd-4adf-caa9-3f00-b88c41f48543-461f5497\" class=\"rich-text-block-5 w-richtext\"\u003e\n\u003cp\u003e\u003cem\u003e** Non-mammalian collagens may result in a reduced limit of detection. We recommend use of a collagen standard matched to the non-mammalian species under assay.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eMany customers have found that the straightforward sample processing and analysis of Sircol make it a good alternative to conventional hydroxyproline analysis.\u003c\/p\u003e\n\u003ch2\u003eKit Contents\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe standard size Sircol™ Soluble Collagen Assay kit (cat. no. S1000) contains:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSircol Dye Reagent (1x 110 ml)\u003c\/li\u003e\n\u003cli\u003eCollagen Reference Standard (1x 5 ml, 0.5 mg\/ml soluble Bovine collagen)\u003c\/li\u003e\n\u003cli\u003eAcid-Salt Wash Reagent (1x 20 ml)\u003c\/li\u003e\n\u003cli\u003eCollagen Isolation and concentration Reagent (1x 20 ml)\u003c\/li\u003e\n\u003cli\u003eAlkali Reagent (1x 110 ml)\u003c\/li\u003e\n\u003cli\u003eAcid-Neutralising Reagent (1x 20 ml)\u003c\/li\u003e\n\u003cli\u003e1.5 ml micro-centrifuge tubes for dye-labelling reaction.\u003c\/li\u003e\n\u003cli\u003eAssay kit manual\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003ch2\u003eWhich Sircol Collagen Assay should I use for my application?\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"Chart to determine which Sircol™ Collagen Assay is best for your application.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/SIRCOL-flow_insoluble_soluble.png?v=1621167801\"\u003e\u003c\/div\u003e\n\u003ch2\u003eResources\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Sircol_Soluble_Collagen_Assay_Manual.pdf?v=1707152828\" title=\"Product Datasheet (PDS): Biocolor Sircol™ Soluble Collagen Assay Manual\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Sircol-Soluble-Collagen-Assay-Kit-sds-en-eu-v9.pdf?v=1628199575\" title=\"MSDS for Sircol™ Soluble Collagen Assay and Components\" target=\"_blank\"\u003e\u003cspan\u003eMaterial Safety Data Sheet (MSDS)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Biocolor Sircol™ Soluble Collagen Assay (Original Version) - Frequently Asked Questions (FAQ)\" href=\"https:\/\/ilexlife.com\/pages\/biocolor-sircol-soluble-collagen-assay-s1000-frequently-asked-questions-faq\" target=\"_blank\"\u003e\u003cspan\u003eFrequently Asked Questions (FAQ)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e2025 Product References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eYu, Sheeline et al. “Cyclic GMP-AMP synthase expression is enhanced in systemic sclerosis-associated interstitial lung disease and stimulates inflammatory myofibroblast activation.” \u003ci\u003eThe European respiratory journal\u003c\/i\u003e vol. 66,2 2401564. 8 Aug. 2025, \u003ca rel=\"noopener\" title=\"Yu, Sheeline et al. “Cyclic GMP-AMP synthase expression is enhanced in systemic sclerosis-associated interstitial lung disease and stimulates inflammatory myofibroblast activation.” The European respiratory journal vol. 66,2 2401564. 8 Aug. 2025, doi:10.1183\/13993003.01564-2024\" href=\"https:\/\/doi.org\/10.1183\/13993003.01564-2024\" target=\"_blank\"\u003edoi:10.1183\/13993003.01564-2024\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eZhou, Jialiang et al. “Decellularized Adipose Matrix Rejuvenates Photoaged Skin through Immune Microenvironment Modulation.” \u003ci\u003eBME frontiers\u003c\/i\u003e vol. 6 0166. 4 Aug. 2025, \u003ca rel=\"noopener\" title=\"Zhou, Jialiang et al. “Decellularized Adipose Matrix Rejuvenates Photoaged Skin through Immune Microenvironment Modulation.” BME frontiers vol. 6 0166. 4 Aug. 2025, doi:10.34133\/bmef.0166\" href=\"https:\/\/doi.org\/10.34133\/bmef.0166\" target=\"_blank\"\u003edoi:10.34133\/bmef.0166\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eJin, Zhengxin et al. “Pantothenic acid ameliorates hepatic fibrosis by targeting IGFBP6 to regulate the TGF-β\/SMADs pathway.” \u003ci\u003eCommunications biology\u003c\/i\u003e vol. 8,1 1127. 29 Jul. 2025, \u003ca href=\"https:\/\/doi.org\/10.1038\/s42003-025-08527-5\" title=\"Jin, Zhengxin et al. “Pantothenic acid ameliorates hepatic fibrosis by targeting IGFBP6 to regulate the TGF-β\/SMADs pathway.” Communications biology vol. 8,1 1127. 29 Jul. 2025, doi:10.1038\/s42003-025-08527-5\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1038\/s42003-025-08527-5\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSong, Minwoo et al. “A Hierarchical Short Microneedle-Cupping Dual-Amplified Patch Enables Accelerated, Uniform, Pain-Free Transdermal Delivery of Extracellular Vesicles.” \u003ci\u003eNano-micro letters\u003c\/i\u003e vol. 18,1 11. 23 Jul. 2025, \u003ca rel=\"noopener\" title=\"Song, Minwoo et al. “A Hierarchical Short Microneedle-Cupping Dual-Amplified Patch Enables Accelerated, Uniform, Pain-Free Transdermal Delivery of Extracellular Vesicles.” Nano-micro letters vol. 18,1 11. 23 Jul. 2025, doi:10.1007\/s40820-025-01853-7\" href=\"https:\/\/doi.org\/10.1007\/s40820-025-01853-7\" target=\"_blank\"\u003edoi:10.1007\/s40820-025-01853-7\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eHong, Seung Hee et al. “Photodynamically tunable ROS-generating hydrogels for accelerated tissue regeneration.” \u003ci\u003eBioactive materials\u003c\/i\u003e vol. 51 977-992. 8 Jul. 2025, \u003ca href=\"https:\/\/doi.org\/10.1016\/j.bioactmat.2025.05.006\" title=\"Hong, Seung Hee et al. “Photodynamically tunable ROS-generating hydrogels for accelerated tissue regeneration.” Bioactive materials vol. 51 977-992. 8 Jul. 2025, doi:10.1016\/j.bioactmat.2025.05.006\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1016\/j.bioactmat.2025.05.006\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eKim, Hyunsik et al. “Loss of p300 in proximal tubular cells reduces renal fibrosis and endothelial-mesenchymal transition.” \u003ci\u003eEMBO molecular medicine\u003c\/i\u003e vol. 17,7 (2025): 1575-1598. \u003ca href=\"https:\/\/doi.org\/10.1038\/s44321-025-00243-1\" title=\"Kim, Hyunsik et al. “Loss of p300 in proximal tubular cells reduces renal fibrosis and endothelial-mesenchymal transition.” EMBO molecular medicine vol. 17,7 (2025): 1575-1598. doi:10.1038\/s44321-025-00243-1\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1038\/s44321-025-00243-1\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eNtinopoulou, Maria et al. “IL-1b-Bearing NETs: Bridging Inflammation to Early Cirrhosis in Hepatitis B.” \u003ci\u003eInternational journal of molecular sciences\u003c\/i\u003e vol. 26,12 5733. 15 Jun. 2025, \u003ca rel=\"noopener\" title=\"Ntinopoulou, Maria et al. “IL-1b-Bearing NETs: Bridging Inflammation to Early Cirrhosis in Hepatitis B.” International journal of molecular sciences vol. 26,12 5733. 15 Jun. 2025, doi:10.3390\/ijms26125733\" href=\"https:\/\/doi.org\/10.3390\/ijms26125733\" target=\"_blank\"\u003edoi:10.3390\/ijms26125733\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBozzini, Sara et al. “Hyaluronic Acid‐Decorated Liposomes for the Intrapulmonary Delivery of Imatinib: A Targeted Treatment for Postinflammatory Pulmonary Fibrosis.” \u003ci\u003eSmall Science\u003c\/i\u003e vol. 5,8 2500144. 10 Jun. 2025, \u003ca href=\"https:\/\/doi.org\/10.1002\/smsc.202500144\" title=\"Bozzini, Sara et al. “Hyaluronic Acid‐Decorated Liposomes for the Intrapulmonary Delivery of Imatinib: A Targeted Treatment for Postinflammatory Pulmonary Fibrosis.” Small Science vol. 5,8 2500144. 10 Jun. 2025, doi:10.1002\/smsc.202500144\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1002\/smsc.202500144\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eVera, Renzo E et al. “Paracrine regulation of pancreatic cancer cell response to chemotherapy by GLI2-collagen I signaling.” \u003ci\u003eThe Journal of biological chemistry\u003c\/i\u003e vol. 301,7 (2025): 110311. \u003ca rel=\"noopener\" title=\"Vera, Renzo E et al. “Paracrine regulation of pancreatic cancer cell response to chemotherapy by GLI2-collagen I signaling.” The Journal of biological chemistry vol. 301,7 (2025): 110311. doi:10.1016\/j.jbc.2025.110311\" href=\"https:\/\/doi.org\/10.1016\/j.jbc.2025.110311\" target=\"_blank\"\u003edoi:10.1016\/j.jbc.2025.110311\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eHussein, Kamal H et al. “Efficacy of xenogeneic fresh and lyophilized amniotic membranes on the healing of experimentally induced full-thickness skin wounds in dogs.” \u003c\/span\u003e\u003ci\u003eScientific reports\u003c\/i\u003e\u003cspan\u003e vol. 15,1 15605. 4 May. 2025, \u003ca rel=\"noopener\" title=\"Hussein, Kamal H et al. “Efficacy of xenogeneic fresh and lyophilized amniotic membranes on the healing of experimentally induced full-thickness skin wounds in dogs.” Scientific reports vol. 15,1 15605. 4 May. 2025, doi:10.1038\/s41598-025-95023-9\" href=\"https:\/\/doi.org\/10.1038\/s41598-025-95023-9\" target=\"_blank\"\u003edoi:10.1038\/s41598-025-95023-9\u003c\/a\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eTang, Chang et al. “Molecular Hydrogen Ameliorates Anti-Desmoglein 1 Antibody-Induced Pemphigus-Associated Interstitial Lung Disease by Inhibiting Oxidative Stress.” \u003ci\u003eInternational journal of molecular sciences\u003c\/i\u003e vol. 26,9 4203. 28 Apr. 2025, \u003ca href=\"https:\/\/doi.org\/10.3390\/ijms26094203\" title=\"Tang, Chang et al. “Molecular Hydrogen Ameliorates Anti-Desmoglein 1 Antibody-Induced Pemphigus-Associated Interstitial Lung Disease by Inhibiting Oxidative Stress.” International journal of molecular sciences vol. 26,9 4203. 28 Apr. 2025, doi:10.3390\/ijms26094203\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.3390\/ijms26094203\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eZhang, Sixuan et al. “Probiotics promote cellular wound healing responses by modulating the PI3K and TGF-β\/Smad signaling pathways.” \u003ci\u003eCell communication and signaling : CCS\u003c\/i\u003e vol. 23,1 195. 23 Apr. 2025, \u003ca href=\"https:\/\/doi.org\/10.1186\/s12964-025-02179-y\" title=\"Zhang, Sixuan et al. “Probiotics promote cellular wound healing responses by modulating the PI3K and TGF-β\/Smad signaling pathways.” Cell communication and signaling : CCS vol. 23,1 195. 23 Apr. 2025, doi:10.1186\/s12964-025-02179-y\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1186\/s12964-025-02179-y\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eXu, Yan et al. “CBD-conjugated BMP-inhibiting exosomes on collagen scaffold dual-target Achilles tendon repair: Synergistic regeneration and heterotopic ossification prevention.” \u003ci\u003eMaterials today. Bio\u003c\/i\u003e vol. 32 101790. 22 Apr. 2025, \u003ca rel=\"noopener\" title=\"Xu, Yan et al. “CBD-conjugated BMP-inhibiting exosomes on collagen scaffold dual-target Achilles tendon repair: Synergistic regeneration and heterotopic ossification prevention.” Materials today. Bio vol. 32 101790. 22 Apr. 2025, doi:10.1016\/j.mtbio.2025.101790\" href=\"https:\/\/doi.org\/10.1016\/j.mtbio.2025.101790\" target=\"_blank\"\u003edoi:10.1016\/j.mtbio.2025.101790\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eXie, Yuanyuan et al. “Identifying TNFSF4\u003csup\u003elow\u003c\/sup\u003e-MSCs superiorly treating idiopathic pulmonary fibrosis through Tregs differentiation modulation.” \u003ci\u003eStem cell research \u0026amp; therapy\u003c\/i\u003e vol. 16,1 194. 20 Apr. 2025, \u003ca rel=\"noopener\" title=\"Xie, Yuanyuan et al. “Identifying TNFSF4low-MSCs superiorly treating idiopathic pulmonary fibrosis through Tregs differentiation modulation.” Stem cell research \u0026amp; therapy vol. 16,1 194. 20 Apr. 2025, doi:10.1186\/s13287-025-04313-6\" href=\"https:\/\/doi.org\/10.1186\/s13287-025-04313-6\" target=\"_blank\"\u003edoi:10.1186\/s13287-025-04313-6\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eShi, Qingqiang et al. “MFGE8 regulates the EndoMT of HLMECs through the BMP signaling pathway and fibrosis in acute lung injury.” \u003ci\u003eRespiratory research\u003c\/i\u003e vol. 26,1 142. 13 Apr. 2025, \u003ca href=\"https:\/\/doi.org\/10.1186\/s12931-025-03215-8\" title=\"Shi, Qingqiang et al. “MFGE8 regulates the EndoMT of HLMECs through the BMP signaling pathway and fibrosis in acute lung injury.” Respiratory research vol. 26,1 142. 13 Apr. 2025, doi:10.1186\/s12931-025-03215-8\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1186\/s12931-025-03215-8\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSrakhao, Waritorn et al. “Therapeutic Potential of Cannabidiol Cyclodextrin Complex in Polymeric Micelle and Tetrahydrocurcumin Cyclodextrin Complex Loaded in Hydrogel to Treat Lymphedema.” \u003ci\u003eInternational journal of molecular sciences\u003c\/i\u003e vol. 26,7 3428. 6 Apr. 2025, \u003ca rel=\"noopener\" title=\"Srakhao, Waritorn et al. “Therapeutic Potential of Cannabidiol Cyclodextrin Complex in Polymeric Micelle and Tetrahydrocurcumin Cyclodextrin Complex Loaded in Hydrogel to Treat Lymphedema.” International journal of molecular sciences vol. 26,7 3428. 6 Apr. 2025, doi:10.3390\/ijms26073428\" href=\"https:\/\/doi.org\/10.3390\/ijms26073428\" target=\"_blank\"\u003edoi:10.3390\/ijms26073428\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eTsilingiris, Dimitrios et al. “Interleukin-8\/Matrix Metalloproteinase-9 Axis Impairs Wound Healing in Type 2 Diabetes through Neutrophil Extracellular Traps-Fibroblast Crosstalk.” \u003ci\u003eEuropean journal of immunology\u003c\/i\u003e vol. 55,4 (2025): e202451664. \u003ca href=\"https:\/\/doi.org\/10.1002\/eji.202451664\" title=\"Tsilingiris, Dimitrios et al. “Interleukin-8\/Matrix Metalloproteinase-9 Axis Impairs Wound Healing in Type 2 Diabetes through Neutrophil Extracellular Traps-Fibroblast Crosstalk.” European journal of immunology vol. 55,4 (2025): e202451664. doi:10.1002\/eji.202451664\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1002\/eji.202451664\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBakhos, Jules Joel et al. “Inhibiting atrial natriuretic peptide clearance reduces myocardial fibrosis and improves cardiac function in diabetic rats.” \u003ci\u003eEuropean heart journal open\u003c\/i\u003e vol. 5,2 oeaf031. 19 Mar. 2025, \u003ca rel=\"noopener\" title=\"Bakhos, Jules Joel et al. “Inhibiting atrial natriuretic peptide clearance reduces myocardial fibrosis and improves cardiac function in diabetic rats.” European heart journal open vol. 5,2 oeaf031. 19 Mar. 2025, doi:10.1093\/ehjopen\/oeaf031\" href=\"https:\/\/doi.org\/10.1093\/ehjopen\/oeaf031\" target=\"_blank\"\u003edoi:10.1093\/ehjopen\/oeaf031\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eShi, Xiangguang et al. “Increased melanin induces aberrant keratinocyte - melanocyte - basal - fibroblast cell communication and fibrogenesis by inducing iron overload and ferroptosis resistance in keloids.” \u003ci\u003eCell communication and signaling : CCS\u003c\/i\u003e vol. 23,1 141. 18 Mar. 2025, \u003ca href=\"https:\/\/doi.org\/10.1186\/s12964-025-02116-z\" title=\"Shi, Xiangguang et al. “Increased melanin induces aberrant keratinocyte - melanocyte - basal - fibroblast cell communication and fibrogenesis by inducing iron overload and ferroptosis resistance in keloids.” Cell communication and signaling : CCS vol. 23,1 141. 18 Mar. 2025, doi:10.1186\/s12964-025-02116-z\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1186\/s12964-025-02116-z\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eZhang, Zejin et al. “PD-1 inhibition disrupts collagen homeostasis and aggravates cardiac dysfunction through endothelial-fibroblast crosstalk and EndMT.” \u003ci\u003eFrontiers in pharmacology\u003c\/i\u003e vol. 16 1549487. 17 Mar. 2025, \u003ca rel=\"noopener\" title=\"Zhang, Zejin et al. “PD-1 inhibition disrupts collagen homeostasis and aggravates cardiac dysfunction through endothelial-fibroblast crosstalk and EndMT.” Frontiers in pharmacology vol. 16 1549487. 17 Mar. 2025, doi:10.3389\/fphar.2025.1549487\" href=\"https:\/\/doi.org\/10.3389\/fphar.2025.1549487\" target=\"_blank\"\u003edoi:10.3389\/fphar.2025.1549487\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBanaschewski, Brandon J H et al. “Emergence of inflammatory fibroblasts with aging in Hermansky-Pudlak syndrome associated pulmonary fibrosis.” \u003ci\u003eCommunications biology\u003c\/i\u003e vol. 8,1 284. 22 Feb. 2025, \u003ca href=\"https:\/\/doi.org\/10.1038\/s42003-025-07589-9\" title=\"Banaschewski, Brandon J H et al. “Emergence of inflammatory fibroblasts with aging in Hermansky-Pudlak syndrome associated pulmonary fibrosis.” Communications biology vol. 8,1 284. 22 Feb. 2025, doi:10.1038\/s42003-025-07589-9\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1038\/s42003-025-07589-9\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eWong, Keng Lin et al. “Mesenchymal Stem Cell Exosome and Fibrin Sealant Composite Enhances Rabbit Anterior Cruciate Ligament Repair.” \u003ci\u003eThe American journal of sports medicine\u003c\/i\u003e vol. 53,4 (2025): 871-884. \u003ca rel=\"noopener\" title=\"Wong, Keng Lin et al. “Mesenchymal Stem Cell Exosome and Fibrin Sealant Composite Enhances Rabbit Anterior Cruciate Ligament Repair.” The American journal of sports medicine vol. 53,4 (2025): 871-884. doi:10.1177\/03635465241313142\" href=\"https:\/\/doi.org\/10.1177\/03635465241313142\" target=\"_blank\"\u003edoi:10.1177\/03635465241313142\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eChen, Shuang et al. “Exploring the correlation between magnetic resonance diffusion tensor imaging (DTI) parameters and aquaporin expression and biochemical composition content in degenerative intervertebral disc nucleus pulposus tissue: a clinical experimental study.” \u003ci\u003eBMC musculoskeletal disorders\u003c\/i\u003e vol. 26,1 157. 17 Feb. 2025, \u003ca href=\"https:\/\/doi.org\/10.1186\/s12891-025-08382-9\" title=\"Chen, Shuang et al. “Exploring the correlation between magnetic resonance diffusion tensor imaging (DTI) parameters and aquaporin expression and biochemical composition content in degenerative intervertebral disc nucleus pulposus tissue: a clinical experimental study.” BMC musculoskeletal disorders vol. 26,1 157. 17 Feb. 2025, doi:10.1186\/s12891-025-08382-9\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1186\/s12891-025-08382-9\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBesio, Roberta et al. “The administration of exogenous HSP47 as a collagen-specific therapeutic approach.” \u003ci\u003eJCI insight\u003c\/i\u003e vol. 10,6 e181570. 6 Feb. 2025, \u003ca rel=\"noopener\" title=\"Besio, Roberta et al. “The administration of exogenous HSP47 as a collagen-specific therapeutic approach.” JCI insight vol. 10,6 e181570. 6 Feb. 2025, doi:10.1172\/jci.insight.181570\" href=\"https:\/\/doi.org\/10.1172\/jci.insight.181570\" target=\"_blank\"\u003edoi:10.1172\/jci.insight.181570\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eKim, Jungbum et al. “Lung-homing nanoliposomes for early intervention in NETosis and inflammation during acute lung injury.” \u003ci\u003eNano convergence\u003c\/i\u003e vol. 12,1 8. 3 Feb. 2025, \u003ca href=\"https:\/\/doi.org\/10.1186\/s40580-025-00475-4\" title=\"Kim, Jungbum et al. “Lung-homing nanoliposomes for early intervention in NETosis and inflammation during acute lung injury.” Nano convergence vol. 12,1 8. 3 Feb. 2025, doi:10.1186\/s40580-025-00475-4\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1186\/s40580-025-00475-4\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSekiguchi, Akiko et al. “Inhibition of skin fibrosis via regulation of Th17\/Treg imbalance in systemic sclerosis.” \u003ci\u003eScientific reports\u003c\/i\u003e vol. 15,1 1423. 9 Jan. 2025, \u003ca rel=\"noopener\" title=\"Sekiguchi, Akiko et al. “Inhibition of skin fibrosis via regulation of Th17\/Treg imbalance in systemic sclerosis.” Scientific reports vol. 15,1 1423. 9 Jan. 2025, doi:10.1038\/s41598-025-85895-2\" href=\"https:\/\/doi.org\/10.1038\/s41598-025-85895-2\" target=\"_blank\"\u003edoi:10.1038\/s41598-025-85895-2\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMcCall, A Scott et al. “Hypoxia-inducible factor 2 regulates alveolar regeneration after repetitive injury in three-dimensional cellular and in vivo models.” \u003ci\u003eScience translational medicine\u003c\/i\u003e vol. 17,780 (2025): eadk8623. \u003ca href=\"https:\/\/doi.org\/10.1126\/scitranslmed.adk8623\" title=\"McCall, A Scott et al. “Hypoxia-inducible factor 2 regulates alveolar regeneration after repetitive injury in three-dimensional cellular and in vivo models.” Science translational medicine vol. 17,780 (2025): eadk8623. doi:10.1126\/scitranslmed.adk8623\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1126\/scitranslmed.adk8623\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eQiu, Huijun et al. “Human Umbilical Cord-Mesenchymal Stem Cells Combined With Low Dosage Nintedanib Rather Than Using Alone Mitigates Pulmonary Fibrosis in Mice.” \u003ci\u003eStem cells international\u003c\/i\u003e vol. 2025 9445735. 7 Jan. 2025, \u003ca rel=\"noopener\" title=\"Qiu, Huijun et al. “Human Umbilical Cord-Mesenchymal Stem Cells Combined With Low Dosage Nintedanib Rather Than Using Alone Mitigates Pulmonary Fibrosis in Mice.” Stem cells international vol. 2025 9445735. 7 Jan. 2025, doi:10.1155\/sci\/9445735\" href=\"https:\/\/doi.org\/10.1155\/sci\/9445735\" target=\"_blank\"\u003edoi:10.1155\/sci\/9445735\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eDagnachew, Yinebeb Mezgebu et al. “Collagen deposition in lung parenchyma driven by depletion of interstitial Lyve-1\u003csup\u003e+\u003c\/sup\u003e macrophages prevents cigarette smoke-induced emphysema and loss of airway function.” \u003ci\u003eFrontiers in immunology\u003c\/i\u003e vol. 15 1493395. 3 Jan. 2025, \u003ca href=\"https:\/\/doi.org\/10.3389\/fimmu.2024.1493395\" title=\"Dagnachew, Yinebeb Mezgebu et al. “Collagen deposition in lung parenchyma driven by depletion of interstitial Lyve-1+ macrophages prevents cigarette smoke-induced emphysema and loss of airway function.” Frontiers in immunology vol. 15 1493395. 3 Jan. 2025, doi:10.3389\/fimmu.2024.1493395\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.3389\/fimmu.2024.1493395\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003eThis product is intended for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003elaboratory research use only\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand is not for use in diagnostic procedures.\u003c\/p\u003e\n\u003ch2\u003eRelated Products\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/biocolor-sircol-2-0-soluble-collagen-assay\" title=\"NEW: Biocolor Sircol™ 2.0 Soluble Collagen Assay (96-well plate format) - product page\" target=\"_blank\"\u003eNEW: Biocolor Sircol™ 2.0 Soluble Collagen Assay\u003c\/a\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cul\u003e\n\u003cli\u003eSircol 2.0™ uses an updated 96-well microplate format with an improved dye formulation to significantly enhance collagen sensitivity and specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cli\u003e\u003ca title=\"Biocolor Sircol™ Insoluble Collagen Assay product page\" href=\"https:\/\/ilexlife.com\/products\/biocolor-sircol-insoluble-collagen-assay\" target=\"_blank\"\u003eBiocolor Sircol™ Insoluble Collagen Assay\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Collagen and Hydroxyproline Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/assays-for-collagen-analysis\" target=\"_blank\"\u003eCollagen and Hydroxyproline Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" title=\"Browse Extracellular Matrix Assays product collection\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" title=\"Extracellular Matrix Molecules product collection\" target=\"_blank\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/promed-bioscience-collagen\" title=\"Promed Bioscience high-purity collagen biomaterials\" rel=\"noopener\" target=\"_blank\"\u003ePromed Bioscience high-purity collagen biomaterials\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of Biocolor products.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biocolor","offers":[{"title":"Standard Size Kit (110 assays), Bovine Standard - S1000","offer_id":39993827950744,"sku":"S1000","price":655.0,"currency_code":"USD","in_stock":true},{"title":"Large Economy Size Kit (440 assays), Bovine Standard - S5000","offer_id":39993828016280,"sku":"S5000","price":1815.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ 2.0 Soluble Collagen Assay (96 wells) - SIRC2","offer_id":44003348512980,"sku":"SIRC2","price":650.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Insoluble\/Soluble Collagen Assays Combination Pack (1X S1000 + 1X S2000) - S4000","offer_id":39993828049048,"sku":"S4000","price":1120.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Dye Reagent (3 x 110 ml) - S1005","offer_id":40361409478808,"sku":"S1005","price":762.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Dye Release Reagent (3 x 110 ml) - S1007","offer_id":40361428254872,"sku":"S1007","price":355.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Dye \u0026 Dye Release Reagents (2 x 110 ml, 2 x 110 ml) - S1500","offer_id":40361349578904,"sku":"S1500","price":785.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Acid-Salt Wash (3 x 20 ml) - SWASH","offer_id":40361247768728,"sku":"SWASH","price":311.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Isolation-Concentration \u0026 Acid Neutralizing Reagents (2 x 2 x 20ml) - SCONC","offer_id":40361221259416,"sku":"SCONC","price":358.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Isolation-Concentration Reagent (3 x 20 ml) - SICR","offer_id":43606368846036,"sku":"SICR","price":311.0,"currency_code":"USD","in_stock":true},{"title":"Sircol™ Bovine Collagen Reference Standard (3 x 5 ml, 0.5 mg\/ml) - S1010","offer_id":40361267232920,"sku":"S1010","price":425.0,"currency_code":"USD","in_stock":true},{"title":"Green Assay Microtube (Refill Pack), 1.5 ml, 100 tubes - T1000","offer_id":47237036998868,"sku":"T1000","price":25.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/S1000kit.png?v=1706908905"},{"product_id":"biocolor-cell-apopercentage-apoptosis-assay","title":"Biocolor APOPercentage™ Apoptosis Assay","description":"\u003cp\u003e\u003cspan style=\"color: rgb(145, 3, 3);\"\u003e\u003cstrong\u003eNote: This product has been discontinued by the manufacturer.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe Biocolor \u003cstrong\u003eCell-APOPercentage™ Apoptosis Assay\u003c\/strong\u003e is a detection and measurement system which allows the user to monitor the occurrence of apoptosis in mammalian, anchorage-dependent cells during in vitro culture.\u003c\/p\u003e\n\u003cp\u003eThe assay uses a dye that is selectively imported by cells that are undergoing apoptosis. Necrotic cells cannot retain the dye and therefore are not stained.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLimit of Detection:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eA single cell (via image analysis method)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Method:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eColorimetric (550 nm) or Image Analysis based\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMeasurements per kit:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eSufficient for 4x24 well plates or 6x96 well plates\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSuitable Samples:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eAdherent mammalian cells (in-vitro)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAssay Run Time:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 hour\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage (unopened kit):\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRoom Temperature (RT)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eBiocolor Ltd.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCountry of Origin:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eUnited Kingdom\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThe mammalian cell membrane has been described as a semi‐fluid mosaic structure, composed of phospholipids with a diverse group of inserted proteins and some cholesterol. The phospholipids are the major components of the membrane and are arranged in the form of a ‘bi‐layer’; which is asymmetric in composition, structure, and function.\u003c\/p\u003e\n\u003cp\u003eTo ensure normal transmembrane functions the phospholipids must be maintained in an asymmetric composition. The process is regulated by ‘flippases’, which catalyse the active transport of aminophospholipids from the outer to inner monolayer. However, in cells undergoing apoptosis, flippase is overwhelmed by the action of another enzyme, termed ‘floppase’ or ‘scramblase’. The net effect is a scrambling of the phospholipid distribution between the inner and outer monolayers.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Apoptosis_Detection_480x480.png?v=1621168661\" alt=\"Diagram Showing The Asymmetric Phospholipid Composition of a Viable and an Apoptotic Mammalian Cell Membrane\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cem\u003eDiagram Showing The Asymmetric Phospholipid Composition of a Viable and an Apoptotic Mammalian Cell Membrane\u003c\/em\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\n\u003cp style=\"text-align: left;\"\u003eThe APOPercentage assay utilizes an intense, pink-colored dye reagent which is taken up during\u003cspan\u003e \u003c\/span\u003e\u003cem\u003ein-vitro\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eculture by apoptosis-committed cells. This uptake occurs at the stage of Phosphatidylserine transmembrane movement, as produced by the flipflop mechanism. Dye uptake continues until \u003cem\u003eblebbing\u003c\/em\u003e occurs. No further dye can then enter the now defunct cell and the dye that has accumulated within the cell is not released (unlike necrotic cells which release dye).\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003eSince the dye reagent is excluded or not retained by healthy or necrotic cells it therefore acts as a specific label for apoptotic cells.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg style=\"float: none;\" alt=\"Biocolor Apopercentage Apoptosis Dyed Cells\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/ApoPercentage_Dyed_cells.jpg?v=1665087369\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eQuantification Method\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eLabelled apoptosis cells may then be conveniently analyzed by the following methods:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDirect Analysis: \u003c\/strong\u003e\u003cspan\u003eThe intense pink color of the labelled cells can be visually assessed using brightfield microscopy. Apoptosis in substrate-adherent cell populations is therefore readily quantified using image analysis techniques. Photomicrograph images obtained are transferred to a computer where the stained area is counted in pixels using downloadable software (\u003ca title=\"ImageJ Image Processing and Analysis Software\" href=\"https:\/\/imagej.nih.gov\/ij\/\" target=\"_blank\"\u003eImageJ\u003c\/a\u003e). This technique is the most sensitive with the ability of detecting one single apoptotic cell per well.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eColorimetry Protocol: \u003c\/strong\u003eDye that accumulates within apoptotic cells is released into solution via addition of Dye Release Reagent. The concentration of this intracellular dye is then measured at 550nm using a microplate colorimeter\/spectrophotometer.\u003c\/p\u003e\n\u003cp\u003eThe assay manual describes the colorimetric protocol in a 24-well plate format. Alternative microwell plates, microscope chamber slides and T-flasks are also suitable for use with the assay.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe APOPercentage assay kit does NOT require the use of a Flow Cytometer.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eVideos on how the Apoptosis Assay Works\u003c\/h2\u003e\n\u003cp\u003e\u003ciframe height=\"538\" width=\"956\" src=\"https:\/\/www.youtube.com\/embed\/NlX06_P_eqU?list=PLA0ED62027B5DCDCF\" title=\"YouTube video player\"\u003e\u003c\/iframe\u003e\u003c\/p\u003e\n\u003ch2\u003eWhat type of Cells can be used\u003c\/h2\u003e\n\u003cp\u003eEstablished cell lines are suitable. Cells should be anchorage-dependent. In order to enhance attachment cells exposed to toxic agents may be grown in gelatin.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eKit Contents\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe APOPercentage™ Apoptosis Assay kit (cat. no. A1000) contains:\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eAPOPercentage Dye (1x 5 ml)\u003c\/li\u003e\n\u003cli\u003eDye Release Reagent (1x 150 ml)\u003c\/li\u003e\n\u003cli\u003ePhosphate Buffered Saline (PBS) (1x 120 ml)\u003c\/li\u003e\n\u003cli\u003e24-well starter plate.\u003c\/li\u003e\n\u003cli\u003eAssay kit manual.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe\u003cstrong\u003e\u003cem\u003e\u003cspan\u003e \u003c\/span\u003eColorimetric Protocol\u003c\/em\u003e\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003erequires a Microplate Colorimeter \/ Spectrophotometer.\u003c\/p\u003e\n\u003cp\u003eAdditional 96-well plates will be required for use when reading dye absorbance values.\u003c\/p\u003e\n\u003cp\u003eThe\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e\u003cem\u003eDirect Detection Protocol\u003c\/em\u003e\u003c\/strong\u003e\u003cspan\u003e r\u003c\/span\u003eequires an inverted stage microscope with an attached digital camera.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eNOTE\u003c\/em\u003e\u003cem\u003e: Additional reagents (typically culture medium and suitable apoptosis treatments) may be required for sample preparation prior to assay. Consult manual or contact us for further details.\u003c\/em\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication note\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eApoptosis assays are widely available, each based on a different stage of the apoptotic process. In some cases apoptosis can be induced without detectable DNA degradation and caspase activity may be reduced at late stage apoptosis. Therefore, running two different apoptosis detection methods simultaneously is recommended to confirm that cell death is occurring due to apoptosis. The Cell-APOPercentage Apoptosis Assay can be used on its own or alongside other apoptosis detection assays, such as a caspase activity assay, a tunnel assay (based on DNA fragmentation) or an annexin-V assay (based on binding of Annexin V to phosphatidyl serine that has translocated to the outside of the cell membrane during apoptosis).\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eUsage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003cstrong\u003elaboratory research use only\u003c\/strong\u003e and is not for use in diagnostic procedures.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eResources\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener noreferrer\" title=\"Product Data Sheet (PDS): Cell-APOPercentage™ Apoptosis Assay Manual, Cell-APOPercentage™ Apoptosis Assay Colorimetric and Photomicroscopy Protocols\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Biocolor_APOPercentage_Apoptosis_Assay_Manual.pdf?v=1707153735\" target=\"_blank\"\u003eProduct Data Sheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Cell-APOPercentage™ Apoptosis Assay Material Safety Data Sheet (MSDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/APOPercentage-Assay-Kit-Apoptosis-Assay-sds-en-eu-v8.pdf?v=1628183445\" target=\"_blank\"\u003e\u003cspan\u003eMaterial Safety Data Sheet (\u003c\/span\u003eMSDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Product References to Cell-APOPercentage™ Apoptosis Assay\" href=\"https:\/\/ilexlife.com\/pages\/biocolor-cell-apopercentage-apoptosis-assay-references\" target=\"_blank\"\u003eProduct References (Extended)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003e2024-2025 Product References\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eSassi, Amel et al. “Low-magnitude high-frequency vibration reduces prostate cancer growth and extravasation \u003ci\u003ein vitro\u003c\/i\u003e.” \u003ci\u003eMechanobiology in medicine\u003c\/i\u003e vol. 2,4 100095. 26 Aug. 2024, \u003ca href=\"https:\/\/doi.org\/10.1016\/j.mbm.2024.100095\" title=\"Sassi, Amel et al. “Low-magnitude high-frequency vibration reduces prostate cancer growth and extravasation in vitro.” Mechanobiology in medicine vol. 2,4 100095. 26 Aug. 2024, doi:10.1016\/j.mbm.2024.100095\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1016\/j.mbm.2024.100095\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eRazuwika, Rufaro et al. “A Cytotoxic Indazole-based Gold(III) Carboxamide Pincer Complex Targeting DNA Through Dual Binding Modes of Groove Binding and Alkylation.” \u003ci\u003eChemistry (Weinheim an der Bergstrasse, Germany)\u003c\/i\u003e vol. 31,27 (2025): e202404345. \u003ca href=\"https:\/\/doi.org\/10.1002\/chem.202404345\" title=\"Razuwika, Rufaro et al. “A Cytotoxic Indazole-based Gold(III) Carboxamide Pincer Complex Targeting DNA Through Dual Binding Modes of Groove Binding and Alkylation.” Chemistry (Weinheim an der Bergstrasse, Germany) vol. 31,27 (2025): e202404345. doi:10.1002\/chem.202404345\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1002\/chem.202404345\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSeaman, Kimberly et al. “Mechanical Loading of Osteocytes via Oscillatory Fluid Flow Regulates Early-Stage PC-3 Prostate Cancer Metastasis to Bone.” \u003ci\u003eAdvanced biology\u003c\/i\u003e vol. 9,4 (2025): e2400824. \u003ca rel=\"noopener\" title=\"Seaman, Kimberly et al. “Mechanical Loading of Osteocytes via Oscillatory Fluid Flow Regulates Early-Stage PC-3 Prostate Cancer Metastasis to Bone.” Advanced biology vol. 9,4 (2025): e2400824. doi:10.1002\/adbi.202400824\" href=\"https:\/\/doi.org\/10.1002\/adbi.202400824\" target=\"_blank\"\u003edoi:10.1002\/adbi.202400824\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eJoseph, Anuja Gracy et al. “A comprehensive apoptotic assessment of niloticin in cervical cancer cells: a tirucallane-type triterpenoid from \u003ci\u003eAphanamixis polystachya\u003c\/i\u003e (Wall.) Parker.” \u003ci\u003eRSC medicinal chemistry\u003c\/i\u003e, vol. 15,10 3444–3459. 8 Aug. 2024, \u003ca href=\"https:\/\/doi.org\/10.1039\/d4md00318g\" title=\"Joseph, Anuja Gracy et al. “A comprehensive apoptotic assessment of niloticin in cervical cancer cells: a tirucallane-type triterpenoid from Aphanamixis polystachya (Wall.) Parker.” RSC medicinal chemistry, vol. 15,10 3444–3459. 8 Aug. 2024, doi:10.1039\/d4md00318g\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1039\/d4md00318g\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSilva, Maria P et al. “BUB1B monoallelic germline variants contribute to prostate cancer predisposition by triggering chromosomal instability.” \u003ci\u003eJournal of biomedical science\u003c\/i\u003e vol. 31,1 74. 16 Jul. 2024, \u003ca rel=\"noopener\" title=\"Silva, Maria P et al. “BUB1B monoallelic germline variants contribute to prostate cancer predisposition by triggering chromosomal instability.” Journal of biomedical science vol. 31,1 74. 16 Jul. 2024, doi:10.1186\/s12929-024-01056-z\" href=\"https:\/\/doi.org\/10.1186\/s12929-024-01056-z\" target=\"_blank\"\u003edoi:10.1186\/s12929-024-01056-z\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eOsmanlıoğlu, Haci Ömer, and Mustafa Nazıroğlu. “Resveratrol Modulates Diabetes-Induced Neuropathic Pain, Apoptosis, and Oxidative Neurotoxicity in Mice Through TRPV4 Channel Inhibition.” \u003ci\u003eMolecular neurobiology\u003c\/i\u003e vol. 61,9 (2024): 7269-7286. \u003ca href=\"https:\/\/doi.org\/10.1007\/s12035-024-04311-4\" title=\"Osmanlıoğlu, Haci Ömer, and Mustafa Nazıroğlu. “Resveratrol Modulates Diabetes-Induced Neuropathic Pain, Apoptosis, and Oxidative Neurotoxicity in Mice Through TRPV4 Channel Inhibition.” Molecular neurobiology vol. 61,9 (2024): 7269-7286. doi:10.1007\/s12035-024-04311-4\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1007\/s12035-024-04311-4\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSchaufelberger, Sara A et al. “2-Methoxyestradiol, an Endogenous 17β-Estradiol Metabolite, Induces Antimitogenic and Apoptotic Actions in Oligodendroglial Precursor Cells and Triggers Endoreduplication via the p53 Pathway.” \u003ci\u003eCells\u003c\/i\u003e vol. 13,13 1086. 22 Jun. 2024, \u003ca rel=\"noopener\" title=\"Schaufelberger, Sara A et al. “2-Methoxyestradiol, an Endogenous 17β-Estradiol Metabolite, Induces Antimitogenic and Apoptotic Actions in Oligodendroglial Precursor Cells and Triggers Endoreduplication via the p53 Pathway.” Cells vol. 13,13 1086. 22 Jun. 2024, doi:10.3390\/cells13131086\" href=\"https:\/\/doi.org\/10.3390\/cells13131086\" target=\"_blank\"\u003edoi:10.3390\/cells13131086\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eTakeuchi, Reiri et al. “18‑α‑glycyrrhetinic acid induces apoptosis in gingival fibroblasts exposed to phenytoin.” \u003ci\u003eExperimental and therapeutic medicine\u003c\/i\u003e vol. 28,1 297. 22 May. 2024, \u003ca href=\"https:\/\/doi.org\/10.3892\/etm.2024.12586\" title=\"Takeuchi, Reiri et al. “18‑α‑glycyrrhetinic acid induces apoptosis in gingival fibroblasts exposed to phenytoin.” Experimental and therapeutic medicine vol. 28,1 297. 22 May. 2024, doi:10.3892\/etm.2024.12586\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.3892\/etm.2024.12586\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/enzymes-for-cell-dissociation\" title=\"Enzymes\/reagents for cell dissociation\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003eTrypsin (for cell detachment)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Cell culture media product collection\" href=\"https:\/\/ilexlife.com\/collections\/cell-culture-media\" target=\"_blank\"\u003e\u003cspan\u003eCell culture media\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eBackground\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eWhat is Apoptosis?\u003c\/strong\u003e\u003cbr\u003eApoptosis is an essentially normal physiological process that removes now redundant, cells, particularly during embryonic development and early growth. In adult animals the process removes cells that are irreparable. The apoptotic process is also involved in many major diseases such as cancer, where transformed tumor cells have their apoptotic process disabled, permitting cell cycling to continue unchecked. In contrast some forms of senile dementia may result from excessive apoptotic induction of neural cells.\u003c\/p\u003e\n\u003cp\u003eThe apoptotic process in mammalian cells is a rapid event (2‐4 hours). Within this short time span an apparently viable cell can be quietly dismantled, to disappear leaving no visible trace of its former existence.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of Biocolor products.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biocolor","offers":[{"title":"APOPercentage Apoptosis Assay - Cat. No. A1000","offer_id":39994180337816,"sku":"A1000","price":520.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A1000kit.png?v=1706898086"},{"product_id":"biocolor-cell-clock-cell-cycle-assay","title":"Biocolor Cell-Clock™ Cell Cycle Assay","description":"\u003ch2 data-mce-fragment=\"1\"\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eNOTE: This product has been discontinued as of January 1st, 2023.\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe\u003cspan\u003e Biocolor \u003c\/span\u003e\u003cstrong\u003eCell-Clock\u003c\/strong\u003e\u003cstrong\u003e™\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eCell Cycle Assay\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eis a live cell detection and measurement system that can be employed to monitor the four major phases of the mammalian cell cycle during\u003cspan\u003e \u003c\/span\u003e\u003cem\u003ein vitro\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eculture. The assay uses a redox dye that is imported by cycling cells.\u003c\/p\u003e\n\u003cp\u003eFollowing dye uptake and incubation a distinct color change occurs within cells, with particular color changes being associated with cells in the G0-G1, S, G2 and M Phases.\u003c\/p\u003e\n\u003cp\u003eThe ratio of cells within the different phases can be calculated using this assay supplemented by visible evidence from photomicrographs.  The calculation of phase ratios is obtained by computer software analysis (ImageJ) of the digitized images of photomicrographs.\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/cell-clock-pic-for-website-300x225_480x480.jpg?v=1621194654\" alt=\"CHO Cells stained with Cell-Clock™ Dye Reagent\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan\u003eCHO Cells stained with CellClock\u003c\/span\u003e\u003cstrong\u003e™\u003c\/strong\u003e\u003cspan\u003e Dye Reagent\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eType\u003c\/h2\u003e\n\u003cp\u003eLive cell detection and measurement system\u003c\/p\u003e\n\u003ch2\u003eSoftware\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe image processing software – ImageJ is available for most operating systems, free of charge, from \u003c\/span\u003e\u003ca href=\"https:\/\/imagej.nih.gov\/ij\/\" target=\"_blank\"\u003ehttps:\/\/imagej.nih.gov\/ij\/\u003c\/a\u003e\u003cspan\u003e. This software can be used to obtain ratios of cells within the different cell cycle phases. Full instructions are available in the product manual.\u003c\/span\u003e \u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cp\u003eCell Cycle Assay, Cell cycle assay kit, Cell Cycle phase determination assay\u003c\/p\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Biocolor Cell-Clock Cell Cycle Assay Manual \/ Product Datasheet (PDS) \/ Protocol\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/cellclock_manual.pdf?v=1621195302\" target=\"_blank\"\u003eProduct Data Sheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"References to Biocolor Cell-Clock™ Cell Cycle Assay (2019)\" href=\"https:\/\/ilexlife.com\/pages\/biocolor-cell-clock-cell-cycle-assay-references-2019\"\u003eReferences to Cell-Clock™ Cell Cycle Assay (2019)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Biocolor Cell-Clock Cell Cycle Assay Material Safety Data Sheet (MSDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Cell-Clock-Cell-Cycle-Assay-Kit-sds-en-eu-v7.pdf?v=1628185436\" target=\"_blank\"\u003e\u003cspan\u003eMaterial Safety Data Sheet (MSDS)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of Biocolor Ltd.\u003c\/em\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003c\/h2\u003e","brand":"Biocolor","offers":[{"title":"Cell-Clock™ Standard Kit (100 assays)","offer_id":39994402013336,"sku":"C1000","price":550.0,"currency_code":"USD","in_stock":false},{"title":"Cell-Clock™ Large Economy Kit (400 assays)","offer_id":39994469482648,"sku":"C2000","price":1575.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/Cell-Clock-Cell-Cycle-Assay-Kit.jpg?v=1631566621"},{"product_id":"fibronectin-rat-purified-protein","title":"Fibronectin Rat, Purified Protein (Plasma)","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cem\u003eFibronectin Rat, Purified Protein\u003c\/em\u003e is purified from rat plasma and has a molecular weight of 220 kDa.\u2029\u003cbr\u003e\u003cbr\u003eFibronectin is useful for the induction of cell attachment to a variety of surfaces including plastic and glass tissue culture labware, petri dishes, coverslips, microcarrier beads, etc. Fibronectin is useful for growth and maintenance of cells in low serum conditions. In general, the reconstituted fibronectin should be diluted with sterile physiological saline or \u003ca href=\"https:\/\/ilexlife.com\/collections\/serum-free-cell-culture-media\" title=\"Serum-Free Cell Culture Media product collection\" target=\"_blank\"\u003eserum-free medium\u003c\/a\u003e to a concentration of 10-50 \u003cspan\u003eµ\u003c\/span\u003eg\/ml.\u003c\/p\u003e\n\u003ch2\u003eSummary\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eFibronectin is an ubiquitous extracellular glycoprotein that exists in a soluble form in body fluids and in an insoluble form in the extracellular matrix. It plays a major role in many important physiological processes, such as embryogenesis, wound healing, hemostasis, thrombosis, blood clotting, and cell migration\/adhesion. Fibronectin consists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix and 2) as a soluble disulphide linked dimer found in the plasma. The plasma form is produced by hepatocytes, and the ECM form is synthesized by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.\u003cbr\u003e\u003cbr\u003eFibronectin's importance during cancer progression has further been shown. It represents an active element in the process of T cell activation in the immune cascade triggered by organ transplantation. Plasma fibronectin level is elevated in severe coronary artery disease. Increased plasma fibronectin levels are related with venous thromboembolism (VTE) particularly in males, and extend the probable association between biomarkers and risk factors for arterial atherothrombosis and VTE.\u003cbr\u003e\u003cbr\u003eFibronectin also takes part as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. Fibronectin organizes cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane- bound Fibronectin receptors on cell surfaces.\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e86088-83-7\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eType\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eNative Protein\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eRat plasma\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eGreater than 95.0%\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003eSterile-filtered white lyophilized (freeze-dried) powder. The Rat Fibronectin was lyophilized from a concentrated 1 mg\/ml solution containing 20mM Tris Cl pH-7.5.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003eIt is recommended to reconstitute the lyophilized Rat Fibronectin in sterile 18M-cm H2O not less than 100 µg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eShips at ambient temperature. Upon receipt, store the product at 4ºC\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eStore the lyophilized fibronectin at 4°C. Upon reconstitution, fibronectin should be stored at 4°C for 2 weeks and for future use below -18°C. Please prevent freeze-thaw cycles.\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cp\u003eCold insoluble globulin, FINC, LETS, MSF, FN\u003c\/p\u003e\n\u003ch2\u003eRESOURCES\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Fibronectin, Rat purified protein Product Datasheet (PDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A1131_FIBRONECTIN_RAT_PURIFIED.pdf?v=1629757628\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRELATED PRODUCTS\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" title=\"Attachment Factors Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/attachment-factors-cell-culture\" target=\"_blank\"\u003eAttachment Factors\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" title=\"Extracellular Matrix Molecules product collection\" target=\"_blank\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"100 μg - Cat. No. A1131100","offer_id":40466120835224,"sku":"A1131100","price":220.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A11311MG","offer_id":40466120867992,"sku":"A11311MG","price":600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A1131_FIBRONECTINRAT_NATIVE.jpg?v=1691089673"},{"product_id":"fibronectin-human-e-coli-recombinant-protein","title":"Fibronectin Human, E. coli Recombinant Protein","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eOur \u003cem\u003eFibronectin Human, E. coli Recombinant Protein\u003c\/em\u003e is a single, non-glycosylated polypeptide chain containing 574 amino acids and has a molecular mass of 62.6 kDa. The Fibronectin is purified by proprietary chromatographic techniques.\u003cbr\u003e\u003cbr\u003eFibronectin is useful for the induction of cell attachment to a variety of surfaces including plastic and glass tissue culture labware, petri dishes, coverslips, microcarrier beads, etc. Fibronectin is useful for growth and maintenance of cells in low serum conditions. In general, the reconstituted fibronectin should be diluted with sterile physiological saline or \u003ca rel=\"noopener noreferrer\" title=\"Serum-Free Cell Culture Media Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/serum-free-cell-culture-media\" target=\"_blank\"\u003eserum-free medium\u003c\/a\u003e to a concentration of 10-50 \u003cspan\u003eµ\u003c\/span\u003eg\/ml.\u003c\/p\u003e\n\u003ch2\u003eSummary\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eFibronectin is an ubiquitous extracellular glycoprotein that exists in a soluble form in body fluids and in an insoluble form in the extracellular matrix. It plays a major role in many important physiological processes, such as embryogenesis, wound healing, hemostasis, thrombosis, blood clotting, and cell migration\/adhesion. Fibronectin consists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix and 2) as a soluble disulphide linked dimer found in the plasma. The plasma form is produced by hepatocytes, and the ECM form is synthesized by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.\u003cbr\u003e\u003cbr\u003eFibronectin's importance during cancer progression has further been shown. It represents an active element in the process of T cell activation in the immune cascade triggered by organ transplantation. Plasma fibronectin level is elevated in severe coronary artery disease. Increased plasma fibronectin levels are related with venous thromboembolism (VTE) particularly in males, and extend the probable association between biomarkers and risk factors for arterial atherothrombosis and VTE.\u003cbr\u003e\u003cbr\u003eFibronectin also takes part as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. Fibronectin organizes cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane- bound Fibronectin receptors on cell surfaces.\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e86088-83-7\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eType\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eRecombinant Protein\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eE. coli\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eGreater than 95.0% as determined by:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eAnalysis by RP-HPLC.\u003c\/li\u003e\n\u003cli\u003eAnalysis by SDS-PAGE.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSterile filtered white lyophilized (freeze-dried) powder. Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 5% Trehalose and 0.02% Tween-20.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003eIt is recommended to reconstitute the lyophilized Fibronectin in sterile 18MΩ-cm H2O not less than 100 µg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/p\u003e\n\u003ch2\u003eBiological Activity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cdiv class=\"product-specs-content\"\u003e\n\u003cp\u003eBioactivity was measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The recommended concentration in this application for this effect is typically 1-5 μg\/cm2. Fibronectin can also be added to the media to support cell spreading at a concentration of 0.5-50 μg\/ml. Optimal concentrations will need to be determined for individual user applications.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature. Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eLyophilized Fibronectin, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Fibronectin should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cp\u003eCold insoluble globulin, FINC, LETS, MSF, FN\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Fibronectin Human, E. coli Recombinant Protein Product Datasheet\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A12621_FIBRONECTIN_HUMAN_E._COLI_REC.pdf?v=1629478028\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener noreferrer\" title=\"Attachment Factors Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/attachment-factors-cell-culture\" target=\"_blank\"\u003eAttachment Factors\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener noreferrer\" title=\"Fibronectin Human, Native Purified Protein product page\" href=\"https:\/\/ilexlife.com\/products\/fibronectin-human-purified-protein\" target=\"_blank\"\u003eFibronectin Human, Native Purified Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener noreferrer\" title=\"Fibronectin Rat, Native Purified Protein product page\" href=\"https:\/\/ilexlife.com\/products\/fibronectin-rat-purified-protein\" target=\"_blank\"\u003eFibronectin Rat, Native Purified Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"100 μg - Cat. No. A12621100","offer_id":40466387337368,"sku":"A12621100","price":220.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A126211MG","offer_id":40466387370136,"sku":"A126211MG","price":1350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A12621_FIBRONECTINHUMAN.jpg?v=1691007362"},{"product_id":"fibronectin-human-purified-protein","title":"Fibronectin Human, Purified Protein (Plasma)","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp class=\"product-specs-content\"\u003e\u003cspan lang=\"EN-US\"\u003e\u003cem\u003eFibronectin Human, Purified Protein\u003c\/em\u003e is a native protein purified from Human plasma. It has a molecular weight of 440 kDa.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cp\u003eFibronectin is useful for the induction of cell attachment to a variety of surfaces including plastic and glass tissue culture labware, petri dishes, coverslips, microcarrier beads, etc. Fibronectin is useful for growth and maintenance of cells in low serum conditions. In general, the reconstituted fibronectin should be diluted with sterile physiological saline or \u003ca title=\"serum-free cell culture media product collection\" href=\"https:\/\/ilexlife.com\/collections\/serum-free-cell-culture-media\" target=\"_blank\"\u003eserum-free medium\u003c\/a\u003e to a concentration of 10-50 \u003cspan\u003eµ\u003c\/span\u003eg\/ml.\u003c\/p\u003e\n\u003ch2\u003eSummary\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eFibronectin is an ubiquitous extracellular glycoprotein that exists in a soluble form in body fluids and in an insoluble form in the extracellular matrix. It plays a major role in many important physiological processes, such as embryogenesis, wound healing, hemostasis, thrombosis, blood clotting, and cell migration\/adhesion. Fibronectin consists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix and 2) as a soluble disulphide linked dimer found in the plasma. The plasma form is produced by hepatocytes, and the ECM form is synthesized by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.\u003cbr\u003e\u003cbr\u003eFibronectin's importance during cancer progression has further been shown. It represents an active element in the process of T cell activation in the immune cascade triggered by organ transplantation. Plasma fibronectin level is elevated in severe coronary artery disease. Increased plasma fibronectin levels are related with venous thromboembolism (VTE) particularly in males, and extend the probable association between biomarkers and risk factors for arterial atherothrombosis and VTE.\u003cbr\u003e\u003cbr\u003eFibronectin also takes part as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. Fibronectin organizes cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane- bound Fibronectin receptors on cell surfaces.\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e86088-83-7\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cp\u003eCold insoluble globulin, FINC, LETS, MSF, FN\u003c\/p\u003e\n\u003ch2\u003eType\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eNative Protein\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eHuman plasma\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003e≥\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003cspan\u003e \u003c\/span\u003e95.0%\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe Human Fibronectin was lyophilized from a non sterile 2 mg\/ml buffer of 10mM sodium phosphate, pH 7.5 and 0.15M NaCl.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003eWe suggest reconstituting 1 mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2 mg\/ml using sterile water. Let stand 1-2 hours. The recommended concentration is 4M-5M urea.\u003c\/p\u003e\n\u003cp\u003eWhen using the protein as an attachment factor, wash the urea off after attaching the fibronectin to the growth surface (plate or dish).\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature. Upon receipt, store the product according to the instructions below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eLyophilized Fibronectin, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibronectin should be stored at 4°C between 2-7 days and for future use below -18°C. \u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eRESOURCES\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Product Datasheet (PDS): Fibronectin, Human purified protein (plasma)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A1448_FIBRONECTIN_HUMAN_PURIFIED_2024.08.pdf?v=1724683462\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRELATED PRODUCTS\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/attachment-factors-cell-culture\" title=\"Attachment Factors Product Collection\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eAttachment Factors\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Extracellular Matrix Molecules product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" target=\"_blank\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"200 μg - Cat. No. A1448200","offer_id":40466533122200,"sku":"A1448200","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A14481MG","offer_id":40466533154968,"sku":"A14481MG","price":255.0,"currency_code":"USD","in_stock":true},{"title":"10 mg - Cat. No. A144810MG","offer_id":43557520146644,"sku":"A144810MG","price":1775.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A1448_FIBRONECTINHUMAN.jpg?v=1691007514"},{"product_id":"matrix-gla-protein-mgp-human-e-coli-recombinant-protein","title":"Matrix Gla Protein (MGP) Human, E. coli Recombinant Protein","description":"\u003ch2\u003e\u003cspan data-mce-fragment=\"1\"\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eMatrix Gla Protein (MGP) Human, E. coli Recombinant Protein\u003c\/em\u003e is a single, non-glycosylated polypeptide chain containing 98 amino acids (20-96) and has a molecular mass of 11.8 kDa. \u003c\/span\u003e\u003cspan\u003eThe MGP is fused to a 20 amino acid His-Tag at N-terminus and is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eMatrix Gla protein (MGP) is a member of a family of vitamin-K2 dependent, Gla-containing proteins. MGP has a high affinity binding to calcium ions, similar to other Gla-containing proteins. The protein acts as an inhibitor of vascular mineralization and plays a role in bone organization. \u003cspan data-mce-fragment=\"1\"\u003eMGP is vital for the regulation of calcification in the extracellular matrix, particularly in cartilage and arteries.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cp class=\"product-specs-content\"\u003eMatrix Gla protein, Cell growth-inhibiting gene 36 protein, GIG36, MGLAP, NTI.\u003c\/p\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003cdiv class=\"product-specs-content\"\u003e\n\u003ch2\u003eApplication Notes\u003c\/h2\u003e\n\u003cdiv class=\"product-specs-content\"\u003eA study by Nishimoto S. K. \u0026amp; Nishimoto M. concluded that \"c\u003cspan data-mce-fragment=\"1\"\u003eells attach and spread better on fibronectin and MGP coated surfaces than to fibronectin alone, even though MGP itself has no cell attachment activity.\" \u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product-specs-content\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product-specs-content\" style=\"text-align: start;\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cimg alt=\"Fig. 1.: MGP augments cell spreading on fibronectin. The graph shows the calculated cell area\/cell for fibronectin coated surfaces compared to fibronectin plus MGP coated surfaces.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MGP_FN_cell_spreading.jpg?v=1626732739\" style=\"float: none;\"\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv class=\"product-specs-content\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong\u003eFig. 1.: MGP augments cell spreading on fibronectin.\u003c\/strong\u003e The graph shows the calculated cell area\/cell for fibronectin coated surfaces compared to fibronectin plus MGP coated surfaces.\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv class=\"product-specs-content\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cp class=\"product-specs-content\" style=\"padding-left: 30px;\"\u003e\u003cstrong\u003eSource: \u003c\/strong\u003eNishimoto, S. K., \u0026amp; Nishimoto, M. (2014). Matrix gla protein binds to fibronectin and enhances cell attachment and spreading on fibronectin. \u003ci data-mce-fragment=\"1\"\u003eInternational journal of cell biology\u003c\/i\u003e, \u003ci data-mce-fragment=\"1\"\u003e2014\u003c\/i\u003e, 807013. \u003ca href=\"https:\/\/doi.org\/10.1155\/2014\/807013\" title=\"https:\/\/doi.org\/10.1155\/2014\/807013\" target=\"_blank\"\u003ehttps:\/\/doi.org\/10.1155\/2014\/807013\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eAmino Acid Sequence\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMGSSHHHHHH SSGLVPRGSH\u003c\/span\u003e\u003cspan\u003e MYESHESMES YELNPFINRR NANTFISPQQ RWRAKVQERI RERSKPVHEL NREACDDYRL CERYAMVYGY NAAYNRYF\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eType\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eRecombinant Protein\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eE. coli\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eGreater than 90%.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp class=\"product-specs-content\"\u003eSterile-filtered colorless solution. The MGP solution (1 mg\/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eShipped on regular ice packs. Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% \u003ca title=\"HSA \/ BSA Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003eAvoid multiple freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eRESOURCES\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A1922_MGP.pdf?v=1629819444\" title=\"Matrix Gla Protein (MGP) Human, E. coli Recombinant Protein Product Datasheet (PDS)\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003eWant to learn more about MGP? We have compiled the links below which contain information that you may find interesting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"UniProt Knowledgebase: UniProtKB - P08493 (MGP_HUMAN): Matrix Gla protein\" href=\"https:\/\/www.uniprot.org\/uniprot\/P08493\" target=\"_blank\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eUniProt Knowledgebase:\u003c\/span\u003e UniProtKB - P08493 (MGP_HUMAN): \u003cspan data-mce-fragment=\"1\" mce-data-marked=\"1\"\u003eM\u003c\/span\u003eatrix Gla protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"PubChem Gene Summary: MGP - matrix Gla protein (human)\" href=\"https:\/\/pubchem.ncbi.nlm.nih.gov\/gene\/4256\" target=\"_blank\"\u003e\u003cspan data-mce-fragment=\"1\"\u003ePubChem Gene Summary:\u003c\/span\u003e MGP - matrix Gla protein (human)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/blogs\/news\/matrix-gla-protein-binds-to-fibronectin\" title=\"Blog: Matrix Gla Protein Binds to Fibronectin and Enhances Cell Attachment and Spreading on Fibronectin\" target=\"_blank\"\u003eBlog: Matrix Gla Protein Binds to Fibronectin and Enhances Cell Attachment and Spreading on Fibronectin\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/products\/fibronectin-human-purified-protein\" title=\"Fibronectin Human, Purified Protein\" target=\"_blank\"\u003eFibronectin Human, Purified Protein\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Ilex Life Sciences","offers":[{"title":"20 μg - Cat. No. A1922020","offer_id":40480941801624,"sku":"A1922020","price":225.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A19221MG","offer_id":40480941834392,"sku":"A19221MG","price":2645.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A1922_MGP.jpg?v=1691513649"},{"product_id":"bovine-serum-albumin-bsa","title":"Bovine Serum Albumin (BSA), Lyophilized Powder","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eOur \u003cem\u003eBovine Serum Albumin (BSA), Lyophilized Powder\u003c\/em\u003e contains 583 amino acid residues derived from the prototypical bovine serum albumin sequence. Suitable for use as a biochemical, excipient (an inert substance used as a diluent or vehicle for a protein), in cell culture media and chromatographic applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eApplications\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eDue to free hydrophobic regions albumins are used to solubilize lipids and stabilize other proteins such as growth factors in tissue culture applications. They are also used as blocking agents in Western blots or ELISA applications and as a protein standard. Albumin has a low molecular weight, is easily soluble in water and contains an excess of acidic amino acids.\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCell and tissue culture media supplement\u003c\/li\u003e\n\u003cli\u003eUsed as serum substitute in cell culture\u003c\/li\u003e\n\u003cli\u003eMedium component for cryopreservation and stem cell culture\u003c\/li\u003e\n\u003cli\u003eStabilizer protein in biochemical preparations\u003c\/li\u003e\n\u003cli\u003eReduces unspecific binding in molecular biology assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%; height: 489.583px;\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 66.4743%; height: 19.5833px;\"\u003e\u003cstrong\u003eProduct Category:\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.2372%; height: 19.5833px;\"\u003e\u003cspan\u003e\u003ca rel=\"noopener\" title=\"Cell Culture Media Supplements Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/cell-culture-media-supplements\" target=\"_blank\"\u003eMedia supplements\u003c\/a\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 66.4743%; height: 19.5833px;\"\u003e\u003cstrong\u003eProduct Type:\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.2372%; height: 19.5833px;\"\u003e\u003cspan\u003e\u003ca rel=\"noopener\" title=\"Human Serum Albumin (HSA) \/ Bovine Serum Albumin (BSA) Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" target=\"_blank\"\u003eHSA \/ BSA\u003c\/a\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 66.4743%; height: 19.5833px;\"\u003e\n\u003cstrong\u003eCAS Registry No.:\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 33.2372%; height: 19.5833px;\"\u003e\u003cspan\u003e9048-46-8\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1667px;\"\u003e\n\u003ctd style=\"width: 66.4743%; height: 39.1667px;\"\u003e\u003cstrong\u003eSource:\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.2372%; height: 39.1667px;\"\u003ePurified from bovine serum.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1667px;\"\u003e\n\u003ctd style=\"width: 66.4743%; height: 39.1667px;\"\u003e\u003cstrong\u003eAppearance:\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.2372%; height: 39.1667px;\"\u003eLyophilized (freeze-dried) yellowish powder.\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 137.083px;\"\u003e\n\u003ctd style=\"width: 66.4743%; height: 137.083px;\"\u003e\u003cstrong\u003eReconstitution:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.2372%; height: 137.083px;\"\u003e\u003cspan\u003eIt is recommended to reconstitute the lyophilized BSA in sterile 18MΩ-cm H2O for 20 minutes at room temperature and at a concentration no greater than 200 mg\/ml.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 66.4743%; height: 19.5833px;\"\u003e\u003cstrong\u003ePurity:\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.2372%; height: 19.5833px;\"\u003e\n\u003cspan lang=\"en\" class=\"ILfuVd\"\u003e\u003cspan class=\"hgKElc\"\u003e\u003c\/span\u003e\u003c\/span\u003eGreater than 97.0%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 66.4743%;\"\u003e\u003cstrong\u003eShipping Condition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.2372%;\"\u003eShipped at ambient temperature. Upon receipt, store the product at the temperature recommended below.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 195.833px;\"\u003e\n\u003ctd style=\"width: 66.4743%; height: 195.833px;\"\u003e\u003cstrong\u003eStorage Temperature:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.2372%; height: 195.833px;\"\u003e\u003cspan lang=\"en\" class=\"ILfuVd\"\u003e\u003cspan class=\"hgKElc\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eLyophilized BSA, although stable at room temperature for 3 weeks, should be stored 2-8°C. Upon reconstitution, BSA should be stored at 4°C between 2-7 days and for future use below -18°C. \u003c\/span\u003e\u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003cem\u003e\u003c\/em\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cdiv class=\"product-specs-content\"\u003e\n\u003cp class=\"product-specs-content\"\u003e\u003cspan\u003eAlbumin, ALB, BSA\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A1422_BSA_2024.12.pdf?v=1734459902\" title=\"Bovine Serum Albumin (BSA) Product Datasheet (PDS)\" rel=\"noopener\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBackground\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eAlbumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eRelated Products\u003ca href=\"https:\/\/ilexlife.com\/products\/human-serum-albumin-hsa-lyophilized-powder\" title=\"PAN-Biotech Human Serum Albumin (HSA), lyophilized powder product page\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/h2\u003e\n\u003cdiv class=\"product-specs-content\"\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/human-serum-albumin-hsa-lyophilized-powder\" title=\"Human Serum Albumin (HSA), Lyophilized Powder, US Origin\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eHuman Serum Albumin (HSA), Lyophilized Powder, US Origin\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"PAN-Biotech Human Serum Albumin (HSA), Fatty Acid Free, lyophilized powder product page\" href=\"https:\/\/ilexlife.com\/products\/pan-biotech-human-serum-albumin-hsa-fatty-acid-free-lyophilized-powder\"\u003eHuman Serum Albumin (HSA),\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eFatty Acid Free\u003c\/strong\u003e, Lyophilized Powder\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/products\/pan-biotech-human-serum-albumin-hsa-20-solution-premium-grade-us-origin\" title=\"Human Serum Albumin (HSA), 20% Solution, Premium Grade, US Origin\" rel=\"noopener\" target=\"_blank\"\u003eHuman Serum Albumin (HSA), 20% Solution, Premium Grade, US Origin\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"Recombinant Human Albumin (rHA), Liquid Solution, GMP Grade\" href=\"https:\/\/ilexlife.com\/products\/recombinant-human-albumin-rha-liquid-solution-gmp-grade\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Recombinant Human Albumin (rHA), expressed in rice, lyophilized powder\" href=\"https:\/\/ilexlife.com\/products\/recombinant-human-albumin-rha-expressed-in-rice-lyophilized-powder\" target=\"_blank\"\u003eRecombinant Human Albumin (rHA), expressed in rice, lyophilized powder\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of PAN-Biotech GmbH.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Ilex Life Sciences","offers":[{"title":"10 g - Cat. No. A1422010","offer_id":40508725657752,"sku":"A1422010","price":155.0,"currency_code":"USD","in_stock":true},{"title":"50 g - Cat. No. A1422050","offer_id":40508725690520,"sku":"A1422050","price":425.0,"currency_code":"USD","in_stock":true},{"title":"100 g - Cat. No. A1422100","offer_id":40508819800216,"sku":"A1422100","price":610.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/A1422_BSA.jpg?v=1678133912"},{"product_id":"cardiac-actin-bovine-heart","title":"Actin, Alpha Cardiac Muscle 1, Native Protein (Bovine Heart)","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eUltra pure \u003cem\u003eActin, Alpha Cardiac Muscle 1, Native Protein\u003c\/em\u003e is purified from bovine heart and has a molecular mass of 43,000 dalton.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eAlpha-cardiac actin belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in the cardiac actin have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e9064-37-3\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eActin alpha cardiac muscle 1, Alpha-cardiac actin, ACTC1, ACTC, UniProtKB - Q3ZC07 (ACTC_BOVIN)\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eBovine heart.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSterile Filtered White lyophilized (freeze-dried) powder. The protein was lyophilized from a 1 mg\/ml solution containing 10mM Tris-acetate buffer pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 1 mM DTT and 0.5% SDS.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eGreater than 98.0%.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eIt is recommended to reconstitute the lyophilized Cardiac Actin in sterile 18MΩ-cm H2O not less than 100 µg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature. Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eLyophilized Cardiac-Actin, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Cardiac Actin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% \u003ca href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" title=\"HSA \/ BSA PRODUCT COLLECTION\" target=\"_blank\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eProtein standard in 1D and 2D SDS gelelectrophoresis\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eImmunoassays\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eImmunization\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eRESOURCES\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A1519_C-Actin.pdf?v=1629473267\" title=\"Actin, Alpha Cardiac Muscle 1, Bovine Heart Product Datasheet (PDS)\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Actin, Alpha Skeletal Muscle, Rabbit Protein\" href=\"https:\/\/ilexlife.com\/products\/actin-protein-rabbit-muscle\" target=\"_blank\"\u003eActin, Alpha Skeletal Muscle, Rabbit Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"10 µg - Cat. No. A1519010","offer_id":40532610744472,"sku":"A1519010","price":195.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A15191MG","offer_id":40532610777240,"sku":"A15191MG","price":4550.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A1519_ACTINCARDIACBOVINE.jpg?v=1691095189"},{"product_id":"actin-protein-rabbit-muscle","title":"Actin, Alpha Skeletal Muscle, Rabbit Protein","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eActin, Alpha Skeletal Muscle, Rabbit Protein\u003c\/em\u003e is an ultra-pure Actin protein in the alpha-skeletal muscle isoform, purified from rabbit striated muscle. T\u003c\/span\u003e\u003cspan\u003ehe purification method used (according to Spudich \u0026amp; Watts) results in a highly purified protein with a molecular mass of 43,000 dalton.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eActin is a muscle protein localized in the I band of the myofibrils; acting along with myosin, it is responsible for contraction and relaxation of muscle. Each actin protomer binds one molecule of ATP and has one high affinity site for either calcium or magnesium ions, as well as several low affinity sites. Actin exists as a monomer in low salt concentrations, but filaments form rapidly as salt concentration rises, with the consequent hydrolysis of ATP. It occurs in globular (G-actin) and fibrous (F-actin) forms. Actin is found in all eukaryotic cells (except for nematode sperm). Actin is one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Its other functions include cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e9064-37-3\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eAlpha-actin-1, \u003c\/span\u003eACTA1 gene product, actin alpha isoform, UniProtKB - P68135 (ACTS_RABIT)\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eRabbit muscle.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSterile Filtered White lyophilized (freeze-dried) powder. The protein was lyophilized from a 1 mg\/ml solution containing 10 mM Tris\/HCl buffer pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 1 mM DTT and 0.5% (w\/v) SDS.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eGreater than 98.0%.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eIt is recommended to reconstitute the lyophilized Actin in sterile 18MΩ-cm H2O not less than 1 mg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature. Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eLyophilized Actin, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Actin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% \u003ca href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" title=\"HSA \/ BSA PRODUCT COLLECTION\" target=\"_blank\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A1517_Actin.pdf?v=1629475158\" title=\"Actin Protein, Rabbit Muscle, Product Datasheet (PDS)\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/cardiac-actin-bovine-heart\" title=\"Actin, Alpha Cardiac Muscle 1, Native Protein (Bovine Heart)\" rel=\"noopener\" target=\"_blank\"\u003eActin, Alpha Cardiac Muscle 1, Native Protein (Bovine Heart)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"50 µg - Cat. No. A1517050","offer_id":40532665434264,"sku":"A1517050","price":195.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A15171MG","offer_id":40532665467032,"sku":"A15171MG","price":2000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A1517_ACTINRABBITMUSCLE.jpg?v=1691095295"},{"product_id":"quickzyme-total-collagen-assay","title":"QuickZyme Total Collagen Assay","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eNow collagen can be easily determined by any biochemical laboratory!\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003eQuickZyme Total Collagen Assay\u003c\/strong\u003e kit is based on the quantitative colorimetric determination of hydroxyproline residues, obtained by acid hydrolysis of collagen. Usually for this type of analysis removal of HCl after hydrolysis is needed, which is a time-consuming step and\/or needs special equipment. The QuickZyme Total Collagen Assay is the only hydroxyproline-based assay which does not need this laborious step, resulting in a fast (\u0026lt;2 hrs) and easy (96-well plate format) assay following the hydrolysis step.\u003c\/p\u003e\n\u003cp\u003eCollagen is one of the main components of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Therefore measurement of collagen production is important in many disease related studies.\u003c\/p\u003e\n\u003cp\u003eThe QuickZyme Total Collagen Assay is based on the detection of hydroxyproline. Hydroxyproline is a non-proteinogenic amino acid, which in mammals occurs in elastin and collagen. Its presence is mainly limited to the triple helix of collagen, where its presence increases the triple helix stability. Hydroxyproline is formed posttranslationally from specific proline residues by action of the enzyme prolylhydroxylase. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present.\u003c\/p\u003e\n\u003cp\u003eThe measurement of collagen is started by complete hydrolysis of tissue samples in 6M HCl at 95°C. In the hydrolysate hydroxyproline residues are quantified using a modification of the method described by Prockop and Udenfriend (Anal. Biochem., 1960, 1: 228-239). The assay measures the total amount of hydroxyproline present in the sample, which represents all the types of collagen present in the sample without discriminating between the types of collagen and between procollagen, mature collagen and collagen degradation products.\u003c\/p\u003e\n\u003cp\u003eThe assay is simple and results in a chromogen with an absorbance maximum at 570 nm. The assay is developed in such a way that it doesn’t need the drying step following acid hydrolysis for which often special equipment is needed.\u003c\/p\u003e\n\u003ch2\u003eType\u003c\/h2\u003e\n\u003cp\u003eColorimetric Assay\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Total_Collagen_Principle.jpg?v=1627923600\" alt=\"Assay procedure for QuickZyme Total Collagen Assay\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eCellular extracts,  tissue homogenates, tissues.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Includes:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e100 special screw-capped tubes needed for sample hydrolysis \u003cem\u003e(if you do not need tubes and protocol for sample hydrolysis, you can order \u003ca href=\"https:\/\/ilexlife.com\/products\/quickzyme-hydroxyproline-assay-kit\" title=\"QuickZyme Hydroxyproline Assay\" rel=\"noopener\" target=\"_blank\"\u003eQuickZyme Hydroxyproline Assay\u003c\/a\u003e).\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e2 Adhesive plate seals\u003c\/li\u003e\n\u003cli\u003eAssay buffer\u003c\/li\u003e\n\u003cli\u003e2x Detection reagent A\u003c\/li\u003e\n\u003cli\u003eDetection reagent B\u003c\/li\u003e\n\u003cli\u003eCollagen standard (1200 μg\/ml) in 0.02M acetic acid\u003c\/li\u003e\n\u003cli\u003e96-well assay plate\u003c\/li\u003e\n\u003cli\u003eAssay protocol booklet\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSensitivity\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e2.5 µg\/ml\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eQuantitative Measurement\u003c\/h2\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Total_Collagen_Standard_Curve.jpg?v=1627926119\" alt=\"Example of standard curve with QuickZyme Total Collagen Assay.\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eCollagen Per Total Protein\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eCombination of the \u003ca href=\"https:\/\/ilexlife.com\/products\/quickzyme-total-protein-assay\" title=\"QuickZyme Total Protein Assay\" rel=\"noopener\" target=\"_blank\"\u003e\u003cem\u003eQuickZyme Total Protein Assay\u003c\/em\u003e\u003c\/a\u003e with either the \u003cem\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/quickzyme-total-collagen-assay\" title=\"QuickZyme Total Collagen Assay\" rel=\"noopener\" target=\"_blank\"\u003eTotal Collagen Assay\u003c\/a\u003e\u003c\/em\u003e or \u003cem\u003eHydroxyproline Assay\u003c\/em\u003e allows the expression of data as collagen or hydroxyproline quantity per total amount of protein.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eAssay Run Time\u003c\/h2\u003e\n\u003cp\u003e\u0026lt; 2 hours\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eUnopened kit:\u003c\/strong\u003e Store at room temperature (RT) in the dark. Do not use kit components past kit expiration date.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOpened kit:\u003c\/strong\u003e reconstituted reagents: The opened collagen standard and assay buffer should be stored light protected at 4°C. The other opened reagents should be stored light protected at RT and are stable for at least 1 month. The reconstituted detection reagent (A+B) should be used on the day of reconstitution\u003c\/p\u003e\n\u003ch2\u003eOther Names\/Synonyms\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003ehydroxyproline residues\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Product Datasheet (PDS): Quickzyme Total Collagen Assay Kit Manual \u0026amp; Protocol\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Manual-QuickZyme-total-collagen-assay-Jan2019.pdf?v=1627925532\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Frequently Asked Questions (FAQ) - QuickZyme Assays\" href=\"https:\/\/ilexlife.com\/pages\/quickzyme-biosciences-assays-frequently-asked-questions-faq\" target=\"_blank\"\u003eFrequently Asked Questions (FAQ)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eApplication note 1: \u003c\/span\u003e\u003ca title=\"Collagen analysis: Which assay can be used for what application?\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Application-note-collagen-assays-A4.pdf?v=1628014552\" target=\"_blank\"\u003eHow to choose your collagen assay?\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eApplication note 2: \u003c\/span\u003e\u003ca title=\"Collagen analysis in mouse tissues using various assays\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/application-note-collagen-measurements-in-mouse-tissues-A4.pdf?v=1628014696\" target=\"_blank\"\u003eHow to measure collagen in mouse tissues?\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eApplication note 3: \u003c\/span\u003e\u003ca title=\"Collagen per protein analysis in fresh, frozen and formalin-fixed tissues\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/application-note-protein-assay-applications-1.pdf?v=1628014843\" target=\"_blank\"\u003eCollagen per protein analysis in fresh, frozen and formalin-fixed tissues\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eApplication note 4: \u003ca title=\"Collagen analysis in fish tissue using the total collagen assay\" href=\"https:\/\/ilexlife.com\/pages\/fish-tissue-quickzyme-total-collagen-assay\" target=\"_blank\"\u003eCollagen analysis in fish tissue\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener noreferrer\" title=\"QuickZyme Total Collagen Assay Product References\" href=\"https:\/\/ilexlife.com\/pages\/quickzyme-total-collagen-assay-hydroxyproline-assay-product-references\" target=\"_blank\"\u003eTotal Collagen Assay Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"QuickZyme Total Protein Assay\" href=\"https:\/\/ilexlife.com\/products\/quickzyme-total-protein-assay\" target=\"_blank\"\u003eQuickZyme Total Protein Assay\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"null\" href=\"https:\/\/ilexlife.com\/collections\/assays-for-collagen-analysis\" title=\"Collagen and Hydroxyproline Assays product collection\" target=\"_blank\"\u003eCollagen and Hydroxyproline Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" title=\"Browse Extracellular Matrix Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"null\" title=\"Extracellular Matrix Molecules product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" target=\"_blank\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of QuickZyme Biosciences.\u003c\/em\u003e\u003c\/p\u003e","brand":"QuickZyme","offers":[{"title":"QuickZyme Total Collagen Assay, 1 plate (96 wells) - Cat. No. QZBTOTCOL1","offer_id":40566855631000,"sku":"QZBTOTCOL1","price":599.0,"currency_code":"USD","in_stock":true},{"title":"QuickZyme Total Collagen Assay, 2 plates (2 x 96 wells) - Cat. No. QZBTOTCOL2","offer_id":40566855663768,"sku":"QZBTOTCOL2","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"QuickZyme Total Collagen Assay, 5 plates (5 x 96 wells) - Cat. No. QZBTOTCOL5","offer_id":40566855696536,"sku":"QZBTOTCOL5","price":2230.0,"currency_code":"USD","in_stock":true},{"title":"Total Collagen (QZBTOTCOL1) + Total Protein (QZBTOTPROT1) Assays Combination Pack - Cat. No. QZBTOTCOMBO","offer_id":46170840137940,"sku":"QZBTOTCOMBO","price":1110.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/IMG_2986.png?v=1778177623"},{"product_id":"quickzyme-hydroxyproline-assay-kit","title":"QuickZyme Hydroxyproline Assay Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eQuantitative hydroxyproline measurement for collagen analysis in all types of tissues, in a fast \u0026amp; simple 96-well plate format!\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eDysregulation in collagen metabolism may result in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Therefore measurement of collagen production is important in many disease related studies.\u003c\/p\u003e\n\u003cp\u003eHydroxyproline is a non-proteinogenic amino acid, which in mammals occurs in elastin and collagen. Its presence is mainly limited to the triple helix of collagen, where it leads to increased triple helix stability. Hydroxyproline is formed post-translationally from specific proline residues by action of the enzyme prolylhydroxylase. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present.\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003eQuickZyme Hydroxyproline Assay\u003c\/strong\u003e is a modification of the method described by Prockop and Udenfriend (Anal. Biochem.,1960, 1: 228- 239). The assay measures the total amount of hydroxyproline present in the sample. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all the types of collagen present in the sample without discriminating between the types of collagen and between procollagen, mature collagen and collagen degradation products.\u003c\/p\u003e\n\u003cp\u003eThe assay is simple and results in a chromogen with an absorbance maximum at 570 nm. The assay is developed to measure hydroxyproline in acid tissue and\/or protein hydrolysates in such a way that it doesn’t need the drying step following acid hydrolysis of collagen for which often special equipment is needed. The QuickZyme Hydroxyproline Assay kit is the only hydroxyproline-based assay which does not need this laborious step, resulting in a fast (\u0026lt;2 hrs), sensitive and easy (96-well plate format) assay.\u003c\/p\u003e\n\u003ch2\u003eType\u003c\/h2\u003e\n\u003cp\u003eColorimetric Assay\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Total_Collagen_Principle.jpg?v=1627923600\" alt=\"QuickZyme Hydroxyproline Assay Kit principle.\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003every broad application: conditioned media cells \/ cell extracts tissues \/ tissue extracts species independent collagen type independent\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit includes:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e2x adhesive plate seals\u003c\/li\u003e\n\u003cli\u003eAssay buffer\u003c\/li\u003e\n\u003cli\u003e2x Detection reagent A\u003c\/li\u003e\n\u003cli\u003eDetection reagent B\u003c\/li\u003e\n\u003cli\u003eHydroxyproline standard (3 mM) in sterile water\u003c\/li\u003e\n\u003cli\u003e96-well assay plate\u003c\/li\u003e\n\u003cli\u003eAssay protocol booklet\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eNOTE: \u003cspan\u003eThis kit does not contain tubes or a protocol for collagen hydrolysis. If you need these items please order the \u003ca title=\"QuickZyme Total Collagen Assay Kit product page\" href=\"https:\/\/ilexlife.com\/products\/quickzyme-total-collagen-assay\" target=\"_blank\"\u003eQuickZyme Total Collagen Assay\u003c\/a\u003e.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eSensitivity\u003c\/h2\u003e\n\u003cp\u003e2.4 µM  \u003cspan\u003e(0.3 µg\/ml)\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eQuantitative Measurement\u003c\/h2\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Hydroxyproline_Standard_Curve.png?v=1627927768\" alt=\"QuickZyme Hydroxyproline Assay Kit quantitative measurement.\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003ch2\u003eCollagen Per Total Protein\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eCombination of the \u003ca rel=\"noopener\" title=\"QuickZyme Total Protein Assay\" href=\"https:\/\/ilexlife.com\/products\/quickzyme-total-protein-assay\" target=\"_blank\"\u003e\u003cem\u003eQuickZyme Total Protein Assay\u003c\/em\u003e\u003c\/a\u003e with either the \u003cem\u003e\u003ca rel=\"noopener\" title=\"QuickZyme Total Collagen Assay\" href=\"https:\/\/ilexlife.com\/products\/quickzyme-total-collagen-assay\" target=\"_blank\"\u003eTotal Collagen Assay\u003c\/a\u003e\u003c\/em\u003e or \u003cem\u003eHydroxyproline Assay\u003c\/em\u003e allows the expression of data as collagen or hydroxyproline quantity per total amount of protein.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eAssay Run Time\u003c\/h2\u003e\n\u003cp\u003e\u0026lt; 2 hours\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eUnopened kit:\u003c\/strong\u003e Store at room temperature (RT) in the dark. Do not use kit components past kit expiration date.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOpened kit:\u003c\/strong\u003e reconstituted reagents: The opened collagen standard and assay buffer should be stored light protected at 4°C. The other opened reagents should be stored light protected at RT and are stable for at least 1 month. The reconstituted detection reagent (A+B) should be used on the day of reconstitution\u003c\/p\u003e\n\u003ch2\u003eOther Names\/Synonyms\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003ehydroxyproline residues\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Manual-QuickZyme-hydroxyproline-assay-Jan-2019.pdf?v=1627928062\" title=\"Product Datasheet (PDS): Quickzyme Hydroxyproline Assay Manual and Protocol\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Frequently Asked Questions (FAQ) - QuickZyme Assays\" href=\"https:\/\/ilexlife.com\/pages\/quickzyme-biosciences-assays-frequently-asked-questions-faq\" target=\"_blank\"\u003eFrequently Asked Questions (FAQ)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eApplication note 1: \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Application-note-collagen-assays-A4.pdf?v=1628014552\" title=\"Collagen analysis: Which assay can be used for what application?\" target=\"_blank\"\u003eHow to choose your collagen assay?\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eApplication note 2: \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/application-note-collagen-measurements-in-mouse-tissues-A4.pdf?v=1628014696\" title=\"Collagen analysis in mouse tissues using various assays\" target=\"_blank\"\u003eHow to measure collagen in mouse tissues?\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eApplication note 3: \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/application-note-protein-assay-applications-1.pdf?v=1628014843\" title=\"Collagen per protein analysis in fresh, frozen and formalin-fixed tissues\" target=\"_blank\"\u003eCollagen per protein analysis in fresh, frozen and formalin-fixed tissues\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eApplication note 4:\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/fish-tissue-quickzyme-total-collagen-assay\" title=\"Collagen analysis in fish tissue using the total collagen assay\" target=\"_blank\"\u003eCollagen analysis in fish tissue\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener noreferrer\" title=\"QuickZyme Hydroxyproline Assay Product References\" href=\"https:\/\/ilexlife.com\/pages\/quickzyme-total-collagen-assay-hydroxyproline-assay-product-references\" target=\"_blank\"\u003eHydroxyproline Assay Product References\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/quickzyme-total-protein-assay\" title=\"QuickZyme Total Protein Assay\" rel=\"noopener\" target=\"_blank\"\u003eQuickZyme Total Protein Assay\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Collagen and Hydroxyproline Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/assays-for-collagen-analysis\" rel=\"null\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eCollagen and Hydroxyproline Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" title=\"Browse Extracellular Matrix Assays product collection\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" title=\"Extracellular Matrix Molecules product collection\" rel=\"null\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of QuickZyme Biosciences.\u003c\/em\u003e\u003c\/p\u003e","brand":"QuickZyme","offers":[{"title":"1 plate (96 wells) - Cat. No. QZBHYPRO1","offer_id":40567289774232,"sku":"QZBHYPRO1","price":589.0,"currency_code":"USD","in_stock":true},{"title":"2 plates (2 x 96 wells) - Cat. No. QZBHYPRO2","offer_id":40567289807000,"sku":"QZBHYPRO2","price":1090.0,"currency_code":"USD","in_stock":true},{"title":"5 plates (5 x 96 wells) - Cat. No. QZBHYPRO5","offer_id":40567289839768,"sku":"QZBHYPRO5","price":2188.0,"currency_code":"USD","in_stock":true},{"title":"Hydroxyproline (QZBHYPRO1) + Total Protein (QZBTOTPROT1) Assays Combination Pack - Cat. No. QZBHYPCOMBO","offer_id":46171025670356,"sku":"QZBHYPCOMBO","price":1090.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/QZBHYPRO1.png?v=1675963381"},{"product_id":"quickzyme-total-protein-assay","title":"QuickZyme Total Protein Assay","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eQuantitative measurement of total protein in acid hydrolysates of fresh, frozen, fixed and paraffin embedded tissues in a fast and simple 96-well plate format!\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe \u003ca rel=\"noopener\" title=\"QuickZyme Total Collagen Assay Kit\" href=\"https:\/\/ilexlife.com\/products\/quickzyme-total-collagen-assay\" target=\"_blank\"\u003e\u003cem\u003eQuickZyme Total Collagen Assay\u003c\/em\u003e\u003c\/a\u003e or \u003ca href=\"https:\/\/ilexlife.com\/products\/quickzyme-hydroxyproline-assay-kit\" title=\"QuickZyme Hydroxyproline Assay Kit\" rel=\"noopener\" target=\"_blank\"\u003e\u003cem\u003eQuickZyme Hydroxyproline Assay\u003c\/em\u003e\u003c\/a\u003e are frequently used to determine the amount of collagen in biological samples such as tissues, tissue extracts, cell extracts, and culture media. For the proper interpretation of the data obtained these should be compared to some reference quantity like wet- or dry- weight of the tissue, amount of protein, DNA etc. Some of these quantities are not easy to measure, or require an additional sample because they cannot be measured directly in acid hydrolysates. It would be helpful if it was possible to determine such a reference in the acid hydrolysate also used for determination of total collagen or hydroxyproline, such that no longer additional samples or cumbersome methods would be required. The \u003cstrong\u003e\u003cem\u003eQuickZyme Total Protein Assay\u003c\/em\u003e\u003c\/strong\u003e offers a convenient solution in such cases.\u003c\/p\u003e\n\u003cp\u003eIn this assay the total amount of amino acids present in the same acid hydrolysate used in the QuickZyme Total Collagen and Hydroxyproline Assays is easily determined. This total amount of amino acids in the hydrolysate is a direct measure of the amount of protein present in the original sample. Combination of the QuickZyme Total Protein Assay with either the Total Collagen or Hydroxyproline Assay allows the expression of data as collagen or hydroxyproline per total amount of protein.\u003c\/p\u003e\n\u003cp\u003eThe QuickZyme Total Protein Assay is based on the formation of a blue colored product from genipin with free amino acids (Lee et al. Anal. Chim. Acta 480(2003)267; Fujikawa et al. J. Ferment. Technol. 65(1987)149). The assay measures the total amount of amino acids present in the hydrolyzed sample, with exception of proline and hydroxyproline. The amount of amino acids is a good measure for the amount of protein. A hydrolyzed protein standard is supplied with the kit to directly convert data to mg of protein.\u003c\/p\u003e\n\u003cp\u003eThe assay is simple and results in a colored product with an absorbance maximum close to 570 nm. The assay is developed to measure protein in acid tissue and\/or protein hydrolysates in such a way that the assay can be performed directly on the hydrolysate without a drying or neutralization step. When used in addition to the QuickZyme Total Collagen Assay or QuickZyme Hydroxyproline Assay, this assay kit allows for easy expression of the collagen or hydroxyproline amounts per total amount of protein.\u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eQuantitative measurement of protein in acid hydrolysates\u003c\/li\u003e\n\u003cli\u003eSpecies independent\u003c\/li\u003e\n\u003cli\u003eColorimetric read out at 570 nm\u003c\/li\u003e\n\u003cli\u003eBroad dynamic range: 0.05 to 3.0 mg\/ml. Sensitivity: 0.007 mg\/ml\u003c\/li\u003e\n\u003cli\u003eUses BSA as standard\u003c\/li\u003e\n\u003cli\u003eEase-of-use: faster and easier than ELISA\u003c\/li\u003e\n\u003cli\u003eStorage at room temperature\u003c\/li\u003e\n\u003cli\u003eThis kit does not require special equipment\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eType\u003c\/h2\u003e\n\u003cp\u003eColormetric Assay\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg height=\"480x480\" width=\"480x480\" style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/total_protein_assay_principle.png?v=1628102355\" alt=\"QuickZyme Total Protein Assay principle\"\u003e\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eTissue extract, acid hydrolysates of tissues, Conditioned media, Cells\/cell extracts, Frozen or paraffin tissue sections\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSensitivity\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e0.007 mg\/ml\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eQuantitative Measurement\u003c\/h2\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/total_protein_assay_std_curve.png?v=1628102464\" style=\"float: none;\" alt=\"QuickZyme Total Protein Assay: A typical protein standard curve in the range of 0.047-3.00 mg\/ml protein, the background is not subtracted).\"\u003e\u003c\/div\u003e\n\u003cp\u003eA typical protein standard curve in the range of 0.047-3.00 mg\/ml protein, the background is not subtracted).\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eUnopened kit:\u003c\/strong\u003e Store at 4°C in the dark. Do not use kit components past kit expiration date.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOpened kit \/ reconstituted reagents:\u003c\/strong\u003e The reconstituted reagents should be stored light protected at 4°C (\u0026lt; 1 week), longtime storage at -20°C (stable for at least 2 months). The diluted color reagent solution should be prepared fresh and used the same day. \u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Manual-QuickZyme-total-protein-assay-Feb-2018.pdf?v=1628103104\" title=\"Product Datasheet (PDS): Quickzyme Total Protein Assay Manual\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eApplication note 1: \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Application-note-collagen-assays-A4.pdf?v=1628014552\" title=\"Collagen analysis: Which assay can be used for what application?\" target=\"_blank\"\u003eHow to choose your collagen assay?\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eApplication note 2: \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/application-note-collagen-measurements-in-mouse-tissues-A4.pdf?v=1628014696\" title=\"Collagen analysis in mouse tissues using various assays\" target=\"_blank\"\u003eHow to measure collagen in mouse tissues?\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eApplication note 3: \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/application-note-protein-assay-applications-1.pdf?v=1628014843\" title=\"Collagen per protein analysis in fresh, frozen and formalin-fixed tissues\" target=\"_blank\"\u003eCollagen per protein analysis in fresh, frozen and formalin-fixed tissues\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eApplication note 4: \u003ca href=\"https:\/\/ilexlife.com\/pages\/fish-tissue-quickzyme-total-collagen-assay\" title=\"Collagen analysis in fish tissue using the total collagen assay\" target=\"_blank\"\u003eCollagen analysis in fish tissue\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTarnutzer, Katharina et al. “Collagen constitutes about 12% in females and 17% in males of the total protein in mice.” \u003ci\u003eScientific reports\u003c\/i\u003e vol. 13,1 4490. 18 Mar. 2023,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1038%2Fs41598-023-31566-z\" title=\"Tarnutzer, Katharina et al. “Collagen constitutes about 12% in females and 17% in males of the total protein in mice.” Scientific reports vol. 13,1 4490. 18 Mar. 2023, doi:10.1038\/s41598-023-31566-z\" target=\"_blank\"\u003edoi:10.1038\/s41598-023-31566-z\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eGart, Eveline et al. “Translational characterization of the temporal dynamics of metabolic dysfunctions in liver, adipose tissue and the gut during diet-induced NASH development in Ldlr-\/-.Leiden mice.” \u003ci\u003eHeliyon\u003c\/i\u003e vol. 9,3 e13985. 24 Feb. 2023,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1016%2Fj.heliyon.2023.e13985\" title=\"Gart, Eveline et al. “Translational characterization of the temporal dynamics of metabolic dysfunctions in liver, adipose tissue and the gut during diet-induced NASH development in Ldlr-\/-.Leiden mice.” Heliyon vol. 9,3 e13985. 24 Feb. 2023, doi:10.1016\/j.heliyon.2023.e13985\" target=\"_blank\"\u003edoi:10.1016\/j.heliyon.2023.e13985\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003ePeng, Wei et al. “Activin A and CCR2 regulate macrophage function in testicular fibrosis caused by experimental autoimmune orchitis.” \u003ci\u003eCellular and molecular life sciences : CMLS\u003c\/i\u003e vol. 79,12 602. 24 Nov. 2022,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1007%2Fs00018-022-04632-4\" title=\"Peng, Wei et al. “Activin A and CCR2 regulate macrophage function in testicular fibrosis caused by experimental autoimmune orchitis.” Cellular and molecular life sciences : CMLS vol. 79,12 602. 24 Nov. 2022, doi:10.1007\/s00018-022-04632-4\" target=\"_blank\"\u003edoi:10.1007\/s00018-022-04632-4\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eJaeger, Benedikt et al. “Airway basal cells show a dedifferentiated KRT17\u003csup\u003ehigh\u003c\/sup\u003ePhenotype and promote fibrosis in idiopathic pulmonary fibrosis.” \u003ci\u003eNature communications\u003c\/i\u003e vol. 13,1 5637. 26 Sep. 2022,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1038%2Fs41467-022-33193-0\" title=\"Jaeger, Benedikt et al. “Airway basal cells show a dedifferentiated KRT17highPhenotype and promote fibrosis in idiopathic pulmonary fibrosis.” Nature communications vol. 13,1 5637. 26 Sep. 2022, doi:10.1038\/s41467-022-33193-0\" target=\"_blank\"\u003edoi:10.1038\/s41467-022-33193-0\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eSeidel, Florine et al. “Therapeutic Intervention with Anti-Complement Component 5 Antibody Does Not Reduce NASH but Does Attenuate Atherosclerosis and MIF Concentrations in Ldlr-\/-.Leiden Mice.” \u003ci\u003eInternational journal of molecular sciences\u003c\/i\u003e vol. 23,18 10736. 14 Sep. 2022,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.3390%2Fijms231810736\" title=\"Seidel, Florine et al. “Therapeutic Intervention with Anti-Complement Component 5 Antibody Does Not Reduce NASH but Does Attenuate Atherosclerosis and MIF Concentrations in Ldlr-\/-.Leiden Mice.” International journal of molecular sciences vol. 23,18 10736. 14 Sep. 2022, doi:10.3390\/ijms231810736\" target=\"_blank\"\u003edoi:10.3390\/ijms231810736\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eChoi, Sung-Eun et al. “Inhibition of lysyl oxidase‑like 2 ameliorates folic acid‑induced renal tubulointerstitial fibrosis.” \u003ci\u003eExperimental and therapeutic medicine\u003c\/i\u003e vol. 24,5 648. 2 Sep. 2022,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.3892%2Fetm.2022.11585\" title=\"Choi, Sung-Eun et al. “Inhibition of lysyl oxidase‑like 2 ameliorates folic acid‑induced renal tubulointerstitial fibrosis.” Experimental and therapeutic medicine vol. 24,5 648. 2 Sep. 2022, doi:10.3892\/etm.2022.11585\" target=\"_blank\"\u003edoi:10.3892\/etm.2022.11585\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eBrereton, Christopher J et al. “Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosis.” \u003ci\u003eeLife\u003c\/i\u003e vol. 11 e69348. 21 Feb. 2022,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.7554%2FeLife.69348\" title=\"Brereton, Christopher J et al. “Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosis.” eLife vol. 11 e69348. 21 Feb. 2022, doi:10.7554\/eLife.69348\" target=\"_blank\"\u003edoi:10.7554\/eLife.69348\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eMorrison, Martine C et al. “Heat-Inactivated \u003ci\u003eAkkermansia muciniphila\u003c\/i\u003e Improves Gut Permeability but Does Not Prevent Development of Non-Alcoholic Steatohepatitis in Diet-Induced Obese Ldlr-\/-.Leiden Mice.” \u003ci\u003eInternational journal of molecular sciences\u003c\/i\u003e vol. 23,4 2325. 19 Feb. 2022,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.3390%2Fijms23042325\" title=\"Morrison, Martine C et al. “Heat-Inactivated Akkermansia muciniphila Improves Gut Permeability but Does Not Prevent Development of Non-Alcoholic Steatohepatitis in Diet-Induced Obese Ldlr-\/-.Leiden Mice.” International journal of molecular sciences vol. 23,4 2325. 19 Feb. 2022, doi:10.3390\/ijms23042325\" target=\"_blank\"\u003edoi:10.3390\/ijms23042325\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eGart, Eveline et al. “Butyrate Protects against Diet-Induced NASH and Liver Fibrosis and Suppresses Specific Non-Canonical TGF-β Signaling Pathways in Human Hepatic Stellate Cells.” \u003ci\u003eBiomedicines\u003c\/i\u003e vol. 9,12 1954. 20 Dec. 2021,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.3390%2Fbiomedicines9121954\" title=\"Gart, Eveline et al. “Butyrate Protects against Diet-Induced NASH and Liver Fibrosis and Suppresses Specific Non-Canonical TGF-β Signaling Pathways in Human Hepatic Stellate Cells.” Biomedicines vol. 9,12 1954. 20 Dec. 2021, doi:10.3390\/biomedicines9121954\" target=\"_blank\"\u003edoi:10.3390\/biomedicines9121954\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKelly, Shannon C et al. “The right ventricular transcriptome signature in Ossabaw swine with cardiometabolic heart failure: implications for the coronary vasculature.” \u003ci\u003ePhysiological genomics\u003c\/i\u003e vol. 53,3 (2021): 99-115.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1152%2Fphysiolgenomics.00093.2020\" title=\"Kelly, Shannon C et al. “The right ventricular transcriptome signature in Ossabaw swine with cardiometabolic heart failure: implications for the coronary vasculature.” Physiological genomics vol. 53,3 (2021): 99-115. doi:10.1152\/physiolgenomics.00093.2020\" target=\"_blank\"\u003edoi:10.1152\/physiolgenomics.00093.2020\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eSchneider, Jay W et al. “Dysregulated ribonucleoprotein granules promote cardiomyopathy in RBM20 gene-edited pigs.” \u003ci\u003eNature medicine\u003c\/i\u003e vol. 26,11 (2020): 1788-1800.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1038%2Fs41591-020-1087-x\" title=\"Schneider, Jay W et al. “Dysregulated ribonucleoprotein granules promote cardiomyopathy in RBM20 gene-edited pigs.” Nature medicine vol. 26,11 (2020): 1788-1800. doi:10.1038\/s41591-020-1087-x\" target=\"_blank\"\u003edoi:10.1038\/s41591-020-1087-x\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eZou, Jun et al. “Stabilization of hypoxia-inducible factor ameliorates glomerular injury sensitization after tubulointerstitial injury.” \u003ci\u003eKidney international\u003c\/i\u003e vol. 99,3 (2021): 620-631.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1016%2Fj.kint.2020.09.031\" title=\"Zou, Jun et al. “Stabilization of hypoxia-inducible factor ameliorates glomerular injury sensitization after tubulointerstitial injury.” Kidney international vol. 99,3 (2021): 620-631. doi:10.1016\/j.kint.2020.09.031\" target=\"_blank\"\u003edoi:10.1016\/j.kint.2020.09.031\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eZhang, Mingjun et al. “Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs.” \u003ci\u003ePloS one\u003c\/i\u003e vol. 15,3 e0229445. 11 Mar. 2020,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1371%2Fjournal.pone.0229445\" title=\"Zhang, Mingjun et al. “Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs.” PloS one vol. 15,3 e0229445. 11 Mar. 2020, doi:10.1371\/journal.pone.0229445\" target=\"_blank\"\u003edoi:10.1371\/journal.pone.0229445\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eOno, Hiroya et al. “AMPK Complex Activation Promotes Sarcolemmal Repair in Dysferlinopathy.” \u003ci\u003eMolecular therapy : the journal of the American Society of Gene Therapy\u003c\/i\u003e vol. 28,4 (2020): 1133-1153.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1016%2Fj.ymthe.2020.02.006\" title=\"Ono, Hiroya et al. “AMPK Complex Activation Promotes Sarcolemmal Repair in Dysferlinopathy.” Molecular therapy : the journal of the American Society of Gene Therapy vol. 28,4 (2020): 1133-1153. doi:10.1016\/j.ymthe.2020.02.006\" target=\"_blank\"\u003edoi:10.1016\/j.ymthe.2020.02.006\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eJin, Heng et al. “Innate Immune Signaling Contributes to Tubular Cell Senescence in the Glis2 Knockout Mouse Model of Nephronophthisis.” \u003ci\u003eThe American journal of pathology\u003c\/i\u003e vol. 190,1 (2020): 176-189.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1016%2Fj.ajpath.2019.09.013\" title=\"Jin, Heng et al. “Innate Immune Signaling Contributes to Tubular Cell Senescence in the Glis2 Knockout Mouse Model of Nephronophthisis.” The American journal of pathology vol. 190,1 (2020): 176-189. doi:10.1016\/j.ajpath.2019.09.013\" target=\"_blank\"\u003edoi:10.1016\/j.ajpath.2019.09.013\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKim, David H et al. “Calpain 9 as a therapeutic target in TGFβ-induced mesenchymal transition and fibrosis.” \u003ci\u003eScience translational medicine\u003c\/i\u003e vol. 11,501 (2019): eaau2814.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1126%2Fscitranslmed.aau2814\" title=\"Kim, David H et al. “Calpain 9 as a therapeutic target in TGFβ-induced mesenchymal transition and fibrosis.” Science translational medicine vol. 11,501 (2019): eaau2814. doi:10.1126\/scitranslmed.aau2814\" target=\"_blank\"\u003edoi:10.1126\/scitranslmed.aau2814\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eLandolt, Lea et al. “AXL targeting reduces fibrosis development in experimental unilateral ureteral obstruction.” \u003ci\u003ePhysiological reports\u003c\/i\u003e vol. 7,10 (2019): e14091.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.14814%2Fphy2.14091\" title=\"Landolt, Lea et al. “AXL targeting reduces fibrosis development in experimental unilateral ureteral obstruction.” Physiological reports vol. 7,10 (2019): e14091. doi:10.14814\/phy2.14091\" target=\"_blank\"\u003edoi:10.14814\/phy2.14091\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eVaisman, Boris L et al. “LCAT Enzyme Replacement Therapy Reduces LpX and Improves Kidney Function in a Mouse Model of Familial LCAT Deficiency.” \u003ci\u003eThe Journal of pharmacology and experimental therapeutics\u003c\/i\u003e vol. 368,3 (2019): 423-434.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1124%2Fjpet.118.251876\" title=\"Vaisman, Boris L et al. “LCAT Enzyme Replacement Therapy Reduces LpX and Improves Kidney Function in a Mouse Model of Familial LCAT Deficiency.” The Journal of pharmacology and experimental therapeutics vol. 368,3 (2019): 423-434. doi:10.1124\/jpet.118.251876\" target=\"_blank\"\u003edoi:10.1124\/jpet.118.251876\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eJin, Heng et al. “Epithelial innate immunity mediates tubular cell senescence after kidney injury.” \u003ci\u003eJCI insight\u003c\/i\u003e vol. 4,2 e125490. 24 Jan. 2019,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1172%2Fjci.insight.125490\" title=\"Jin, Heng et al. “Epithelial innate immunity mediates tubular cell senescence after kidney injury.” JCI insight vol. 4,2 e125490. 24 Jan. 2019, doi:10.1172\/jci.insight.125490\" target=\"_blank\"\u003edoi:10.1172\/jci.insight.125490\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eTeuscher, Alina C et al. “Assessing Collagen Deposition During Aging in Mammalian Tissue and in Caenorhabditis elegans.” \u003ci\u003eMethods in molecular biology (Clifton, N.J.)\u003c\/i\u003e vol. 1944 (2019): 169-188.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1007%2F978-1-4939-9095-5_13\" title=\"Teuscher, Alina C et al. “Assessing Collagen Deposition During Aging in Mammalian Tissue and in Caenorhabditis elegans.” Methods in molecular biology (Clifton, N.J.) vol. 1944 (2019): 169-188. doi:10.1007\/978-1-4939-9095-5_13\" target=\"_blank\"\u003edoi:10.1007\/978-1-4939-9095-5_13\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eSchilter, Heidi et al. “The lysyl oxidase like 2\/3 enzymatic inhibitor, PXS-5153A, reduces crosslinks and ameliorates fibrosis.” \u003ci\u003eJournal of cellular and molecular medicine\u003c\/i\u003e vol. 23,3 (2019): 1759-1770.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1111%2Fjcmm.14074\" title=\"Schilter, Heidi et al. “The lysyl oxidase like 2\/3 enzymatic inhibitor, PXS-5153A, reduces crosslinks and ameliorates fibrosis.” Journal of cellular and molecular medicine vol. 23,3 (2019): 1759-1770. doi:10.1111\/jcmm.14074\" target=\"_blank\"\u003edoi:10.1111\/jcmm.14074\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eJones, Mark G et al. “Nanoscale dysregulation of collagen structure-function disrupts mechano-homeostasis and mediates pulmonary fibrosis.” \u003ci\u003eeLife\u003c\/i\u003e vol. 7 e36354. 3 Jul. 2018,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.7554%2FeLife.36354\" title=\"Jones, Mark G et al. “Nanoscale dysregulation of collagen structure-function disrupts mechano-homeostasis and mediates pulmonary fibrosis.” eLife vol. 7 e36354. 3 Jul. 2018, doi:10.7554\/eLife.36354\" target=\"_blank\"\u003edoi:10.7554\/eLife.36354\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eVeDepo, Mitchell et al. “Comparison of Candidate Cell Populations for the Recellularization of Decellularized Heart Valves.” \u003ci\u003eCellular and molecular bioengineering\u003c\/i\u003e vol. 11,3 197-209. 30 Apr. 2018,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1007%2Fs12195-018-0524-0\" title=\"VeDepo, Mitchell et al. “Comparison of Candidate Cell Populations for the Recellularization of Decellularized Heart Valves.” Cellular and molecular bioengineering vol. 11,3 197-209. 30 Apr. 2018, doi:10.1007\/s12195-018-0524-0\" target=\"_blank\"\u003edoi:10.1007\/s12195-018-0524-0\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/quickzyme-total-collagen-assay\" title=\"QuickZyme Total Collagen Assay product page\" target=\"_blank\"\u003eQuickZyme Total Collagen Assay\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/quickzyme-hydroxyproline-assay-kit\" title=\"QuickZyme Hydroxyproline Assay product page\" target=\"_blank\"\u003eQuickZyme Hydroxyproline Assay\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/quickzyme-sensitive-tissue-collagen-assay\" title=\"QuickZyme Sensitive Tissue Collagen Assay product page\" target=\"_blank\"\u003eQuickZyme Sensitive Tissue Collagen Assay\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/quickzyme-sensitive-tissue-hydroxyproline-assay\" title=\"QuickZyme Sensitive Tissue Hydroxyproline Assay product page\" target=\"_blank\"\u003eQuickZyme Sensitive Tissue Hydroxyproline Assay\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Browse Extracellular Matrix Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of QuickZyme Biosciences.\u003c\/em\u003e\u003c\/p\u003e","brand":"QuickZyme","offers":[{"title":"1 plate (96 wells) - Cat. No. QZBTOTPROT1","offer_id":40581252219032,"sku":"QZBTOTPROT1","price":545.0,"currency_code":"USD","in_stock":true},{"title":"2 plates (2 x 96 wells) - Cat. No. QZBTOTPROT2","offer_id":40581252251800,"sku":"QZBTOTPROT2","price":985.0,"currency_code":"USD","in_stock":true},{"title":"5 plates (5 x 96 wells) - Cat. No. QZBTOTPROT5","offer_id":40581252284568,"sku":"QZBTOTPROT5","price":1930.0,"currency_code":"USD","in_stock":true},{"title":"Total Collagen (QZBTOTCOL1) + Total Protein (QZBTOTPROT1) Assays Combination Pack - Cat. No. QZBTOTCOMBO","offer_id":46170898497748,"sku":"QZBTOTCOMBO","price":1110.0,"currency_code":"USD","in_stock":true},{"title":"Hydroxyproline (QZBHYPRO1) + Total Protein (QZBTOTPROT1) Assays Combination Pack - Cat. No. QZBHYPCOMBO","offer_id":46171099136212,"sku":"QZBHYPCOMBO","price":1090.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/IMG_2983.png?v=1778176671"},{"product_id":"screenmatrix","title":"screenMATRIX - biomatrix screening tool for cell cultures","description":"\u003cdiv class=\"fusion-title title fusion-title-1 fusion-sep-none fusion-title-text fusion-title-size-two fusion-border-below-title\"\u003e\n\u003cp class=\"fusion-responsive-typography-calculated\" data-lineheight=\"49.6px\" data-fontsize=\"40\"\u003e\u003cspan\u003e\u003cspan style=\"color: rgb(255, 0, 0);\"\u003e\u003cstrong\u003eNOTE: This product has been discontinued.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 class=\"fusion-responsive-typography-calculated\" data-lineheight=\"49.6px\" data-fontsize=\"40\"\u003e\u003cspan\u003eYOUR CHALLENGE\u003c\/span\u003e\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cp class=\"fusion-sep-clear\"\u003eYou want to improve your research using a relevant microenvironment for your cells, however the variety of products on the market is huge. To find out which one is the best for you is both time consuming and costly. You don’t want to test out multiple products and waste time reading lengthy protocols. Ideally, you want a product that provides you with biologically relevant microenvironments for your cells and reproducible results.\u003c\/p\u003e\n\u003cdiv class=\"fusion-text fusion-text-6\"\u003e\n\u003ch2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"40\" data-lineheight=\"49.6px\"\u003eA simple tool to find the best environment for any cell type\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"fusion-text fusion-text-7\" style=\"text-align: start;\"\u003e\n\u003cp\u003eWe provide you with the ideal tool to rapidly test a large variety of cell microenvironments at once. denovoMATRIX technology allows us to incorporate the most important biological ligands for cell adhesion, spreading and robust growth within our biomimetic coatings. We have compiled 96 coating compositions which represent 96 different microenvironments in the screenMATRIX. Find out which biological ligands are responsible for cell adhesion, differentiation or changes in your cell phenotype. Turn pipetting into publications, and interest into insight.\u003c\/p\u003e\n\u003cp\u003e \u003cimg height=\"480x480\" width=\"480x480\" alt=\"screenMATRIX cell culture screening tool by denovoMATRIX\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/myMATRIX_iPSC_image_480x480.jpg?v=1628276986\" style=\"float: none;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eFormat\u003c\/h2\u003e\n\u003cp\u003e5X 96-well precoated plates\u003c\/p\u003e\n\u003cp\u003eThe screenMATRIX is assembled using the glycosaminoglycan sugars (GAGs) dextran sulfate, heparin, chondroitin and dermatan. With the exception of the synthetic dextran sulfate, these molecules are naturally present in the extracellular matrix (ECM) and have important roles in signaling as well as growth factor binding. Peptides, which mimic various ECM proteins, complete the composition of the screenMATRIX. Some recapitulate important ECM adhesion proteins such as fibronectin, vitronectin, laminin and collagen, others mimic signaling proteins such as bone morphogenic protein, fibroblast growth factor and transforming growth factor (among others).\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg height=\"2048x2048\" width=\"2048x2048\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/screenMATRIX_plate_layout_2048x2048.png?v=1628524190\" alt=\"Precoated screenMATRIX plate well coatings layout\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eVideo on screenMATRIX \u003c\/h2\u003e\n\u003cp\u003e\u003ciframe title=\"YouTube video player\" src=\"https:\/\/www.youtube.com\/embed\/3bc0arrkNUM\" height=\"619\" width=\"1100\"\u003e\u003c\/iframe\u003e\u003c\/p\u003e\n\u003ch2\u003eReady to use, fast and efficient\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cdiv class=\"fusion-text fusion-text-9\"\u003e\n\u003cp class=\"p2\"\u003e\u003cspan class=\"s1\"\u003eThe screenMATRIX is ready to use without any preparation. All you need to do is seed your cells on the screenMATRIX and discover which coating works best. Check out our screenMATRIX info sheet to find out exactly which biological ligands are responsible for your optimal cell response.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cdiv class=\"fusion-text fusion-text-9\"\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/screenMATRIX_infosheet_S1001_V2__Ilex_edited.pdf?v=1628278151\" title=\"denonoMATRIX screenMATRIX Product Info Sheet and Cell-Seeding Protocol\" target=\"_blank\"\u003escreenMATRIX Product Info Sheet and Cell-Seeding Protocol\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MSDS-screenMATRIX-20210413.pdf?v=1631039188\" title=\"denonoMATRIX screenMATRIX Material Safety Data Sheet (MSDS)\" target=\"_blank\"\u003escreenMATRIX Material Safety Data Sheet (MSDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"screenMATRIX Application Note: AI-assisted Imaging Accelerates Selection of Optimal Cell Culture Matrices\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/appnote-screenmatrix.pdf?v=1658425884\" target=\"_blank\"\u003escreenMATRIX Application Note: AI-assisted Imaging Accelerates Selection of Optimal Cell Culture Matrices\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTondera, Christoph et al. “Highly Conductive, Stretchable, and Cell-Adhesive Hydrogel by Nanoclay Doping.” \u003ci\u003eSmall (Weinheim an der Bergstrasse, Germany)\u003c\/i\u003e vol. 15,27 (2019): e1901406. \u003ca href=\"https:\/\/doi.org\/10.1002\/smll.201901406\" title=\"Tondera, Christoph et al. “Highly Conductive, Stretchable, and Cell-Adhesive Hydrogel by Nanoclay Doping.” Small (Weinheim an der Bergstrasse, Germany) vol. 15,27 (2019): e1901406. doi:10.1002\/smll.201901406\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.1002\/smll.201901406\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003ePanella, Stefano et al. “Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells.” \u003ci\u003eCells\u003c\/i\u003e vol. 10,2 466. 22 Feb. 2021, \u003ca href=\"https:\/\/doi.org\/10.3390\/cells10020466\" title=\"Panella, Stefano et al. “Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells.” Cells vol. 10,2 466. 22 Feb. 2021, doi:10.3390\/cells10020466\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.3390\/cells10020466\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 data-lineheight=\"49.6px\" data-fontsize=\"40\" class=\"\"\u003e\n\u003cspan\u003eFound your ideal coating? \u003c\/span\u003e\u003cspan\u003eGet your favorite coating in a format of your choice – your \u003c\/span\u003e\u003cspan\u003ecustomMATRIX\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eDid you find one or multiple microenvironments strongly performing in your cell culture? Let us know. The denovoMATRIX system is extremely scalable and modular. \u003c\/span\u003e\u003cspan\u003eGet your favorite cell culture plasticware format with a coating of your choice or combine multiple coatings into your own special customMATRIX.\u003c\/span\u003e\u003c\/p\u003e","brand":"denovoMATRIX","offers":[{"title":"Default Title","offer_id":40597731541144,"sku":"S1001\/5","price":600.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/screenMATRIXinfoil.png?v=1658425755"},{"product_id":"quickzyme-human-mmp-2-activity-assay","title":"QuickZyme Human MMP-2 Activity Assay","description":"\u003cp data-mce-fragment=\"1\"\u003e\u003cspan style=\"color: rgb(140, 0, 0);\"\u003e\u003cstrong\u003eNOTE: This product has been discontinued by the manufacturer.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eQuantify both endogenous active MMP-2 and total MMP-2\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe \u003cem\u003eQuickZyme Human MMP-2 Activity Assay\u003c\/em\u003e enables you to specifically measure in biological samples both active MMP-2, as well as (pro)MMP-2, which is activated on the plate by APMA. It can be used for the measurement of MMP-2 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine.\u003cbr\u003e\u003cbr\u003eThis 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity.\u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMeasures both endogenous active MMP-2 (naturally occurring) and total active MMP-2 (following activation with APMA).\u003c\/li\u003e\n\u003cli\u003eSamples: cell culture conditioned medium, serum, plasma, urine and tissue homogenates.\u003c\/li\u003e\n\u003cli\u003eQuantitative.\u003c\/li\u003e\n\u003cli\u003eRange: 0.02 – 16 ng\/ml.\u003c\/li\u003e\n\u003cli\u003eSensitivity: 0.04 ng\/ml for 2 h incubation with detection reagent; 0.02 ng\/ml for 6 h incubation with detection reagent; 4 pg\/ml for overnight incubation\u003c\/li\u003e\n\u003cli\u003eEase-of-use: Equivalent to ELISA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eType\u003c\/h2\u003e\n\u003cp\u003eEnzyme activity immunoassay\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg style=\"float: none;\" alt=\"Human MMP-2 Activity Assay principle\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-2_ACTIVITY_76004bfe-dd9c-4544-8de1-d0bf6b1781bf.png?v=1628537505\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003ecell culture conditioned medium, serum, plasma, urine and tissue homogenates\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSensitivity\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e0.04 ng\/ml (2 hr incubation)\u003c\/li\u003e\n\u003cli\u003e0.02 ng\/ml (6 hr incubation)\u003c\/li\u003e\n\u003cli\u003e4 pg\/ml (o\/n incubation)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eQuantitative Measurement\u003c\/h2\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg alt=\"Typical data at 4 hours incubation: QuickZyme Human MMP-2 Activity Assay\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-2_activity_quantification.png?v=1628536871\" width=\"480x480\" height=\"480x480\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp\u003eTypical data with 4 hours incubation.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eUnopened kit: Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date.\u003c\/p\u003e\n\u003cp\u003eOpened kit \/ reconstituted reagents: Please refer to kit manual.\u003c\/p\u003e\n\u003ch2\u003eOther names\u003c\/h2\u003e\n\u003cp\u003eMatrix metalloproteinase-2, Gelatinase A, TBE-1, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, MMP-2, TBE-1, PEX, MMP2 CLG4A\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Manual-QuickZyme-human-MMP-2-activity-assay-V2-June-2017.pdf?v=1628537010\" title=\"Quickzyme Human MMP-2 Activity Assay Manual Product Datasheet (PDS)\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Product-sheet-human-MMP-2-activity-assay.pdf?v=1628537067\" title=\"Brochure for QuickZyme Human MMP-2 Activity Assay\" target=\"_blank\"\u003eProduct Brochure\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/quickzyme-biosciences-assays-frequently-asked-questions-faq\" title=\"Frequently Asked Questions (FAQ) - QuickZyme Assays\" rel=\"noopener\" target=\"_blank\"\u003eFrequently Asked Questions (FAQ)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAncona, Silvia et al. “Differential Modulation of Matrix Metalloproteinases-2 and -7 in LAM\/TSC Cells.” \u003ci\u003eBiomedicines\u003c\/i\u003e vol. 9,12 1760. 24 Nov. 2021,\u003cspan\u003e \u003c\/span\u003e\u003ca title=\"Ancona, Silvia et al. “Differential Modulation of Matrix Metalloproteinases-2 and -7 in LAM\/TSC Cells.” Biomedicines vol. 9,12 1760. 24 Nov. 2021, doi:10.3390\/biomedicines9121760\" href=\"https:\/\/doi.org\/10.3390%2Fbiomedicines9121760\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.3390\/biomedicines9121760\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eNtika, Stelia et al. “Elevated Glucagon-like Peptide-1 and a Th2 Shift May Support Reduced Prevalence of Thoracic Aortic Aneurysm in Patients with Diabetes.” \u003ci\u003eJournal of cardiovascular development and disease\u003c\/i\u003e vol. 8,11 143. 28 Oct. 2021,\u003cspan\u003e \u003c\/span\u003e\u003ca title=\"Ntika, Stelia et al. “Elevated Glucagon-like Peptide-1 and a Th2 Shift May Support Reduced Prevalence of Thoracic Aortic Aneurysm in Patients with Diabetes.” Journal of cardiovascular development and disease vol. 8,11 143. 28 Oct. 2021, doi:10.3390\/jcdd8110143\" href=\"https:\/\/doi.org\/10.3390%2Fjcdd8110143\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.3390\/jcdd8110143\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eRipmeester, Ellen G J et al. “BMP7 reduces the fibrocartilage chondrocyte phenotype.” \u003ci\u003eScientific reports\u003c\/i\u003e vol. 11,1 19663. 4 Oct. 2021,\u003cspan\u003e \u003c\/span\u003e\u003ca title=\"Ripmeester, Ellen G J et al. “BMP7 reduces the fibrocartilage chondrocyte phenotype.” Scientific reports vol. 11,1 19663. 4 Oct. 2021, doi:10.1038\/s41598-021-99096-0\" href=\"https:\/\/doi.org\/10.1038%2Fs41598-021-99096-0\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.1038\/s41598-021-99096-0\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eNtika, Stelia et al. “Syndecan-1 Expression Is Increased in the Aortic Wall of Patients with Type 2 Diabetes but Is Unrelated to Elevated Fasting Plasma Glucagon-Like Peptide-1.” \u003ci\u003eBiomedicines\u003c\/i\u003e vol. 9,6 697. 20 Jun. 2021,\u003cspan\u003e \u003c\/span\u003e\u003ca title=\"Ntika, Stelia et al. “Syndecan-1 Expression Is Increased in the Aortic Wall of Patients with Type 2 Diabetes but Is Unrelated to Elevated Fasting Plasma Glucagon-Like Peptide-1.” Biomedicines vol. 9,6 697. 20 Jun. 2021, doi:10.3390\/biomedicines9060697\" href=\"https:\/\/doi.org\/10.3390%2Fbiomedicines9060697\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.3390\/biomedicines9060697\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eShiroto, Yuki et al. “Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules \u003ci\u003eIn Vitro\u003c\/i\u003e.” \u003ci\u003eActa histochemica et cytochemica\u003c\/i\u003e vol. 54,1 (2021): 1-9.\u003cspan\u003e \u003c\/span\u003e\u003ca title=\"Shiroto, Yuki et al. “Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules In Vitro.” Acta histochemica et cytochemica vol. 54,1 (2021): 1-9. doi:10.1267\/ahc.20-00021\" href=\"https:\/\/doi.org\/10.1267%2Fahc.20-00021\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.1267\/ahc.20-00021\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eWu, Wen-Chi et al. “Do different bariatric surgical procedures influence plasma levels of matrix metalloproteinase-2, -7, and -9 among patients with type 2 diabetes mellitus?.” \u003ci\u003eWorld journal of diabetes\u003c\/i\u003e vol. 11,6 (2020): 252-260.\u003cspan\u003e \u003c\/span\u003e\u003ca title=\"Wu, Wen-Chi et al. “Do different bariatric surgical procedures influence plasma levels of matrix metalloproteinase-2, -7, and -9 among patients with type 2 diabetes mellitus?.” World journal of diabetes vol. 11,6 (2020): 252-260. doi:10.4239\/wjd.v11.i6.252\" href=\"https:\/\/doi.org\/10.4239%2Fwjd.v11.i6.252\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.4239\/wjd.v11.i6.252\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eRuan, Jun Shan et al. “Ursolic Acid Attenuates TGF-β1-Induced Epithelial-Mesenchymal Transition in NSCLC by Targeting Integrin αVβ5\/MMPs Signaling.” \u003ci\u003eOncology research\u003c\/i\u003e vol. 27,5 (2019): 593-600.\u003cspan\u003e \u003c\/span\u003e\u003ca title=\"Ruan, Jun Shan et al. “Ursolic Acid Attenuates TGF-β1-Induced Epithelial-Mesenchymal Transition in NSCLC by Targeting Integrin αVβ5\/MMPs Signaling.” Oncology research vol. 27,5 (2019): 593-600. doi:10.3727\/096504017X15051723858706\" href=\"https:\/\/doi.org\/10.3727%2F096504017X15051723858706\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.3727\/096504017X15051723858706\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Matrix Metalloproteinases Analysis Assays Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/matrix-metalloproteinases-analysis\" target=\"_blank\"\u003eMatrix Metalloproteinases Analysis\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Extracellular Matrix Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of QuickZyme Biosciences.\u003c\/em\u003e\u003c\/p\u003e","brand":"QuickZyme","offers":[{"title":"96 wells - Cat. No. QZBMMP2HV2","offer_id":40615949697176,"sku":"QZBMMP2HV2","price":1595.0,"currency_code":"USD","in_stock":false},{"title":"4 x 96 wells - Cat. No. QZBMMP2HV2X4","offer_id":44239942648020,"sku":"QZBMMP2HV2X4","price":4295.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/MMP-2ACTIVITY.png?v=1628537548"},{"product_id":"quickzyme-human-mmp-7-activity-assay","title":"QuickZyme Human MMP-7 Activity Assay","description":"\u003ch2 data-mce-fragment=\"1\"\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eQuantify both endogenous active MMP-7 and total MMP-7\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe \u003cem\u003eQuickZyme Human MMP-7 Activity Assay\u003c\/em\u003e enables you to specifically measure in biological samples both active human MMP-7, as well as pro-MMP-7, which is activated on the plate by APMA. It can be used for the quantification of MMP-7 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine.\u003cbr\u003e\u003cbr\u003eThis 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity.\u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMeasures both endogenous active MMP-7 (naturally occurring) and total active MMP-7 (following activation with APMA).\u003c\/li\u003e\n\u003cli\u003eSamples: conditioned culture medium, serum, urine and tissue homogenates.\u003c\/li\u003e\n\u003cli\u003eQuantitative.\u003c\/li\u003e\n\u003cli\u003eRange: 0.004 – 16 ng\/ml.\u003c\/li\u003e\n\u003cli\u003eSensitivity: 30 pg\/ml for 3 h incubation with detection reagent; 4 pg\/ml for 25 h incubation with detection reagent.\u003c\/li\u003e\n\u003cli\u003eEase-of-use: Equivalent to ELISA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eType\u003c\/h2\u003e\n\u003cp\u003eEnzyme activity immunoassay\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg style=\"float: none;\" height=\"480x480\" width=\"480x480\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-7_activity.png?v=1628538617\" alt=\"QuickZyme Human MMP-7 Activity Assay principe\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003econditioned culture medium, serum, urine and tissue homogenates\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSensitivity\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e0.03 ng\/ml (3 hr incubation)\u003c\/li\u003e\n\u003cli\u003e0.009 ng\/ml (6 hr incubation)\u003c\/li\u003e\n\u003cli\u003e0.004 ng\/ml (25 hr incubation)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003eQuantitative Measurement\u003c\/h2\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg style=\"float: none;\" height=\"480x480\" width=\"480x480\" alt=\"Typical data with 3 hours incubation: QuickZyme Human MMP-7 Activity Assay\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-7_activity_standard_curve.png?v=1628538706\"\u003e\u003c\/div\u003e\n\u003cp\u003eTypical data with 3 hours incubation. The shown data curve is provided for demonstration only.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eUnopened kit:\u003c\/strong\u003e Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOpened kit \/ reconstituted reagents:\u003c\/strong\u003e Please refer to kit manual.\u003c\/p\u003e\n\u003ch2\u003eOther names\u003c\/h2\u003e\n\u003cp\u003eMatrix metalloproteinase-7, MMP7, Matrilysin, Matrilysin-1, Matrin, Pump-1 protease, Uterine metalloproteinase\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Quickzyme Human MMP-7 Activity Assay Manual Product Datasheet (PDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Manual-QuickZyme-human-MMP-7-activity-assay-Feb-2018.pdf?v=1628539194\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Brochure for QuickZyme Human MMP-7 Activity Assay\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Product-sheet-human-MMP-7-activity-assay-1.pdf?v=1628539206\" target=\"_blank\"\u003e Product Brochure\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Frequently Asked Questions (FAQ) - QuickZyme Assays\" href=\"https:\/\/ilexlife.com\/pages\/quickzyme-biosciences-assays-frequently-asked-questions-faq\" target=\"_blank\"\u003eFrequently Asked Questions (FAQ)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMossa, Abubakr H et al. “Imbalance of nerve growth factor metabolism in aging women with overactive bladder syndrome.” \u003ci\u003eWorld journal of urology\u003c\/i\u003e vol. 39,6 (2021): 2055-2063. \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1007\/s00345-020-03422-6\" title=\"Mossa, Abubakr H et al. “Imbalance of nerve growth factor metabolism in aging women with overactive bladder syndrome.” World journal of urology vol. 39,6 (2021): 2055-2063. doi:10.1007\/s00345-020-03422-6\" target=\"_blank\"\u003edoi:10.1007\/s00345-020-03422-6\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eAncona, Silvia et al. “Differential Modulation of Matrix Metalloproteinases-2 and -7 in LAM\/TSC Cells.” \u003ci\u003eBiomedicines\u003c\/i\u003e vol. 9,12 1760. 24 Nov. 2021,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.3390%2Fbiomedicines9121760\" title=\"Ancona, Silvia et al. “Differential Modulation of Matrix Metalloproteinases-2 and -7 in LAM\/TSC Cells.” Biomedicines vol. 9,12 1760. 24 Nov. 2021, doi:10.3390\/biomedicines9121760\" target=\"_blank\"\u003edoi:10.3390\/biomedicines9121760\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eWu, Wen-Chi et al. “Do different bariatric surgical procedures influence plasma levels of matrix metalloproteinase-2, -7, and -9 among patients with type 2 diabetes mellitus?.” \u003ci\u003eWorld journal of diabetes\u003c\/i\u003e vol. 11,6 (2020): 252-260.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.4239%2Fwjd.v11.i6.252\" title=\"Wu, Wen-Chi et al. “Do different bariatric surgical procedures influence plasma levels of matrix metalloproteinase-2, -7, and -9 among patients with type 2 diabetes mellitus?.” World journal of diabetes vol. 11,6 (2020): 252-260. doi:10.4239\/wjd.v11.i6.252\" target=\"_blank\"\u003edoi:10.4239\/wjd.v11.i6.252\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/matrix-metalloproteinases-analysis\" title=\"Matrix Metalloproteinases Analysis Assays Product Collection\" target=\"_blank\"\u003eMatrix Metalloproteinases Analysis\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" title=\"Extracellular Matrix Assays product collection\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of QuickZyme Biosciences.\u003c\/em\u003e\u003c\/p\u003e","brand":"QuickZyme","offers":[{"title":"96 wells - Cat. No. QZBMMP7H","offer_id":40616161575064,"sku":"QZBMMP7H","price":1630.0,"currency_code":"USD","in_stock":true},{"title":"4 x 96 wells - Cat. No. QZBMMP7HX4","offer_id":44239953002708,"sku":"QZBMMP7HX4","price":4250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/MMP-7activity.png?v=1628538975"},{"product_id":"quickzyme-human-mmp-8-activity-assay","title":"QuickZyme Human MMP-8 Activity Assay","description":"\u003ch2 data-mce-fragment=\"1\"\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eQuantify both endogenous active MMP-8 and total MMP-8\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe \u003cem\u003eQuickZyme Human MMP-8 Activity Assay\u003c\/em\u003e enables you to specifically measure in biological samples both active human MMP-8, as well as (pro)MMP-8 which is activated on the plate by APMA. It can be used for the measurement of MMP-8 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine.\u003cbr\u003e\u003cbr\u003eThis 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity.\u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMeasures both endogenous active MMP-8 (naturally occurring) and total active MMP-8 (following activation with APMA).\u003c\/li\u003e\n\u003cli\u003eSamples: cell culture conditioned medium, serum, plasma, urine, saliva and tissue homogenates.\u003c\/li\u003e\n\u003cli\u003eQuantitative.\u003c\/li\u003e\n\u003cli\u003eRange: 10 – 24000 pg\/ml.\u003c\/li\u003e\n\u003cli\u003eSensitivity: 100 pg\/ml for 2 h incubation with detection reagent; 24 pg\/ml for 6 h incubation with detection reagent; 4 pg\/ml for 24 h incubation with detection reagent.\u003c\/li\u003e\n\u003cli\u003eEase-of-use: Equivalent to ELISA.\u003c\/li\u003e\n\u003cli\u003eMarker for neutrophils (PMN)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eType\u003c\/h2\u003e\n\u003cp\u003eEnzyme activity immunoassay\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg alt=\"QuickZyme Human MMP-8 Activity Assay principle\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-8_Assay_Principle.png?v=1628540165\" width=\"480x480\" height=\"480x480\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ecell culture conditioned medium, serum, plasma, urine, saliva, tissue homogenates\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSensitivity\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e100 pg\/ml (2 hr incubation)\u003c\/li\u003e\n\u003cli\u003e24 pg\/ml (6 hr incubation)\u003c\/li\u003e\n\u003cli\u003e4 pg\/ml (24 hr incubation)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003eQuantitative Measurement\u003c\/h2\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg style=\"float: none;\" height=\"480x480\" width=\"480x480\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-8_typical_data.png?v=1628540579\" alt=\"Typical data with 2 hours incubation: QuickZyme Human MMP-8 Activity Assay\"\u003e\u003c\/div\u003e\n\u003cp\u003eTypical data with 2 hours incubation. The shown data curve is provided for demonstration only.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eUnopened kit: Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date.\u003c\/p\u003e\n\u003cp\u003eOpened kit \/ reconstituted reagents: Please refer to kit manual.\u003c\/p\u003e\n\u003ch2\u003eOther names\u003c\/h2\u003e\n\u003cp\u003eMatrix metalloproteinase-8, MMP8, Neutrophil collagenase, PMNL collagenase, PMNL-CL\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Manual-QuickZyme-human-MMP-8-activity-assay-Feb-2018.pdf?v=1628540279\" title=\"Quickzyme Human MMP-8 Activity Assay Manual Product Datasheet (PDS)\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Product-sheet-human-MMP-8-activity-assay-1.pdf?v=1628540261\" title=\"Brochure for QuickZyme Human MMP-8 Activity Assay\" target=\"_blank\"\u003e Product Brochure\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/quickzyme-biosciences-assays-frequently-asked-questions-faq\" title=\"Frequently Asked Questions (FAQ) - QuickZyme Assays\" rel=\"noopener\" target=\"_blank\"\u003eFrequently Asked Questions (FAQ)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eLee, Jungwon et al. “Diagnostic Models for Screening of Periodontitis with Inflammatory Mediators and Microbial Profiles in Saliva.” \u003ci\u003eDiagnostics (Basel, Switzerland)\u003c\/i\u003e vol. 10,10 820. 14 Oct. 2020,\u003cspan\u003e \u003c\/span\u003e\u003ca title=\"Lee, Jungwon et al. “Diagnostic Models for Screening of Periodontitis with Inflammatory Mediators and Microbial Profiles in Saliva.” Diagnostics (Basel, Switzerland) vol. 10,10 820. 14 Oct. 2020, doi:10.3390\/diagnostics10100820\" href=\"https:\/\/doi.org\/10.3390%2Fdiagnostics10100820\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.3390\/diagnostics10100820\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRELATED PRODUCTS\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"null\" href=\"https:\/\/ilexlife.com\/collections\/matrix-metalloproteinases-analysis\" title=\"Matrix Metalloproteinases Analysis Assays Product Collection\" target=\"_blank\"\u003eMatrix Metalloproteinases Analysis\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"null\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" title=\"Extracellular Matrix Assays product collection\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of QuickZyme Biosciences.\u003c\/em\u003e\u003c\/p\u003e","brand":"QuickZyme","offers":[{"title":"96 wells - Cat. No. QZBMMP8H","offer_id":40616278950040,"sku":"QZBMMP8H","price":1630.0,"currency_code":"USD","in_stock":true},{"title":"4 x 96 wells - Cat. No. QZBMMP8HX4","offer_id":44239967682772,"sku":"QZBMMP8HX4","price":4250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/MMP-8AssayPrinciple.png?v=1628540214"},{"product_id":"quickzyme-human-mmp-9-activity-assay","title":"QuickZyme Human MMP-9 Activity Assay","description":"\u003ch2 data-mce-fragment=\"1\"\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eQuantify both endogenous active MMP-9 and total MMP-9\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe \u003cem\u003eQuickZyme Human MMP-9 Activity Assay\u003c\/em\u003e enables you to specifically measure in biological samples both active human MMP-9, as well as (pro)MMP-9 which is activated on the plate by APMA. It can be used for the measurement of MMP-9 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine.\u003cbr\u003e\u003cbr\u003eThis 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity.\u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMeasures both endogenous active MMP-9 (naturally occurring) and total active MMP-9 (following activation with APMA).\u003c\/li\u003e\n\u003cli\u003eSamples: conditioned cell culture medium, serum, plasma, urine, synovial fluid and tissue homogenates.\u003c\/li\u003e\n\u003cli\u003eQuantitative.\u003c\/li\u003e\n\u003cli\u003eRange: 0.5 to 16 ng\/ml (1 h incubation with detection reagent), 0.01-1 ng\/ml (4 h incubation with detection reagent).\u003c\/li\u003e\n\u003cli\u003eSensitivity: 0.005 ng\/ml.\u003c\/li\u003e\n\u003cli\u003eEase-of-use: Equivalent to ELISA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eType\u003c\/h2\u003e\n\u003cp\u003eEnzyme activity immunoassay\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg alt=\"QuickZyme Human MMP-9 Activity Assay principle\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-9_assay_principle.png?v=1628541713\" width=\"480x480\" height=\"480x480\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003econditioned cell culture medium, serum, plasma, urine, synovial fluid, tissue homogenates\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSensitivity\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e0.1 ng\/ml (1 hr incubation)\u003c\/li\u003e\n\u003cli\u003e0.005 ng\/ml (4 hr incubation)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003eQuantitative Measurement\u003c\/h2\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg alt=\"Typical data with 4 hours incubation: QuickZyme Human MMP-9 Activity Assay\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-9_human_typical_data.png?v=1628541911\" width=\"480x480\" height=\"480x480\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp\u003eTypical data with 4 hours incubation. The shown data curve is provided for demonstration only.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eUnopened kit: Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date.\u003c\/p\u003e\n\u003cp\u003eOpened kit \/ reconstituted reagents: Please refer to kit manual.\u003c\/p\u003e\n\u003ch2\u003eOther names\u003c\/h2\u003e\n\u003cp\u003eMatrix metalloproteinase-9, MMP9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Quickzyme Human MMP-9 Activity Assay Manual Product Datasheet (PDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Manual-QuickZyme-human-MMP-9-activity-assay-Feb-2018.pdf?v=1628541393\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Brochure for QuickZyme Human MMP-9 Activity Assay\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Product-sheet-human-MMP-9-activity-assay-1.pdf?v=1628541393\" target=\"_blank\"\u003eProduct Brochure\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Frequently Asked Questions (FAQ) - QuickZyme Assays\" href=\"https:\/\/ilexlife.com\/pages\/quickzyme-biosciences-assays-frequently-asked-questions-faq\" target=\"_blank\"\u003eFrequently Asked Questions (FAQ)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eRodríguez-Sánchez, Elena et al. “Prediction of the early response to spironolactone in resistant hypertension by the combination of matrix metalloproteinase-9 activity and arterial stiffness parameters.” \u003c\/span\u003e\u003ci data-mce-fragment=\"1\"\u003eEuropean heart journal. Cardiovascular pharmacotherapy\u003c\/i\u003e\u003cspan data-mce-fragment=\"1\"\u003e vol. 8,1 (2022): 68-76. \u003ca title=\"Rodríguez-Sánchez, Elena et al. “Prediction of the early response to spironolactone in resistant hypertension by the combination of matrix metalloproteinase-9 activity and arterial stiffness parameters.” European heart journal. Cardiovascular pharmacotherapy vol. 8,1 (2022): 68-76. doi:10.1093\/ehjcvp\/pvaa086\" href=\"https:\/\/doi.org\/10.1093\/ehjcvp\/pvaa086\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.1093\/ehjcvp\/pvaa086\u003c\/a\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003eKim, Michael D et al. “Vegetable glycerin e-cigarette aerosols cause airway inflammation and ion channel dysfunction.” \u003ci\u003eFrontiers in pharmacology\u003c\/i\u003e vol. 13 1012723. 26 Sep. 2022,\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3389%2Ffphar.2022.1012723\" title=\"Kim, Michael D et al. “Vegetable glycerin e-cigarette aerosols cause airway inflammation and ion channel dysfunction.” Frontiers in pharmacology vol. 13 1012723. 26 Sep. 2022, doi:10.3389\/fphar.2022.1012723\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.3389\/fphar.2022.1012723\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eNoll, Braxton et al. “Regulation of MMP9 transcription by ETS1 in immortalized salivary gland epithelial cells of patients with salivary hypofunction and primary Sjögren's syndrome.” \u003ci\u003eScientific reports\u003c\/i\u003e vol. 12,1 14552. 25 Aug. 2022,\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1038%2Fs41598-022-18576-z\" title=\"Noll, Braxton et al. “Regulation of MMP9 transcription by ETS1 in immortalized salivary gland epithelial cells of patients with salivary hypofunction and primary Sjögren's syndrome.” Scientific reports vol. 12,1 14552. 25 Aug. 2022, doi:10.1038\/s41598-022-18576-z\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.1038\/s41598-022-18576-z\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eMossa, Abubakr H et al. “Imbalance of nerve growth factor metabolism in aging women with overactive bladder syndrome.” \u003ci\u003eWorld journal of urology\u003c\/i\u003e vol. 39,6 (2021): 2055-2063. \u003ca href=\"https:\/\/doi.org\/10.1007\/s00345-020-03422-6\" title=\"Mossa, Abubakr H et al. “Imbalance of nerve growth factor metabolism in aging women with overactive bladder syndrome.” World journal of urology vol. 39,6 (2021): 2055-2063. doi:10.1007\/s00345-020-03422-6\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.1007\/s00345-020-03422-6\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eLauwen, Susette et al. “Increased pro-MMP9 plasma levels are associated with neovascular age-related macular degeneration and with the risk allele of rs142450006 near \u003ci\u003eMMP9\u003c\/i\u003e.” \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8056465\/\" title=\"Lauwen, Susette et al. “Increased pro-MMP9 plasma levels are associated with neovascular age-related macular degeneration and with the risk allele of rs142450006 near MMP9.” Molecular vision vol. 27 142-150. 2 Apr. 2021\" rel=\"noopener noreferrer\" target=\"_blank\"\u003e\u003ci\u003eMolecular vision\u003c\/i\u003e vol. 27 142-150.\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003e2 Apr. 2021\u003c\/li\u003e\n\u003cli\u003eShirakawa, Takuhiro et al. “Dose-Dependent Inhibitory Effect of Rosuvastatin in Japanese Patients with Acute Myocardial Infarction on Serum Concentration of Matrix Metalloproteinases-INVITATION Trial.” \u003ci\u003eJournal of atherosclerosis and thrombosis\u003c\/i\u003e vol. 29,2 (2022): 229-241.\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.5551%2Fjat.59477\" title=\"Shirakawa, Takuhiro et al. “Dose-Dependent Inhibitory Effect of Rosuvastatin in Japanese Patients with Acute Myocardial Infarction on Serum Concentration of Matrix Metalloproteinases-INVITATION Trial.” Journal of atherosclerosis and thrombosis vol. 29,2 (2022): 229-241. doi:10.5551\/jat.59477\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.5551\/jat.59477\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eRingland, Charis et al. “Apolipoprotein E isoforms differentially regulate matrix metallopeptidase 9 function in Alzheimer's disease.” \u003ci\u003eNeurobiology of aging\u003c\/i\u003e vol. 95 (2020): 56-68.\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016%2Fj.neurobiolaging.2020.06.018\" title=\"Ringland, Charis et al. “Apolipoprotein E isoforms differentially regulate matrix metallopeptidase 9 function in Alzheimer's disease.” Neurobiology of aging vol. 95 (2020): 56-68. doi:10.1016\/j.neurobiolaging.2020.06.018\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.1016\/j.neurobiolaging.2020.06.018\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eWu, Wen-Chi et al. “Do different bariatric surgical procedures influence plasma levels of matrix metalloproteinase-2, -7, and -9 among patients with type 2 diabetes mellitus?.” \u003ci\u003eWorld journal of diabetes\u003c\/i\u003e vol. 11,6 (2020): 252-260.\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.4239%2Fwjd.v11.i6.252\" title=\"Wu, Wen-Chi et al. “Do different bariatric surgical procedures influence plasma levels of matrix metalloproteinase-2, -7, and -9 among patients with type 2 diabetes mellitus?.” World journal of diabetes vol. 11,6 (2020): 252-260. doi:10.4239\/wjd.v11.i6.252\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.4239\/wjd.v11.i6.252\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eOishi, Masahiro et al. “Aquaporin 1 elicits cell motility and coordinates vascular bed formation by downregulating thrombospondin type-1 domain-containing 7A in glioblastoma.” \u003ci\u003eCancer medicine\u003c\/i\u003e vol. 9,11 (2020): 3904-3917.\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002%2Fcam4.3032\" title=\"Oishi, Masahiro et al. “Aquaporin 1 elicits cell motility and coordinates vascular bed formation by downregulating thrombospondin type-1 domain-containing 7A in glioblastoma.” Cancer medicine vol. 9,11 (2020): 3904-3917. doi:10.1002\/cam4.3032\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.1002\/cam4.3032\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eRodríguez-Sánchez, Elena et al. “Variations in Circulating Active MMP-9 Levels During Renal Replacement Therapy.” \u003ci\u003eBiomolecules\u003c\/i\u003e vol. 10,4 505. 26 Mar. 2020,\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3390%2Fbiom10040505\" title=\"Rodríguez-Sánchez, Elena et al. “Variations in Circulating Active MMP-9 Levels During Renal Replacement Therapy.” Biomolecules vol. 10,4 505. 26 Mar. 2020, doi:10.3390\/biom10040505\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.3390\/biom10040505\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eGarcía-Prieto, Concha F et al. “Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk.” \u003ci\u003eFrontiers in physiology\u003c\/i\u003e vol. 10 553. 8 May. 2019,\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3389%2Ffphys.2019.00553\" title=\"García-Prieto, Concha F et al. “Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk.” Frontiers in physiology vol. 10 553. 8 May. 2019, doi:10.3389\/fphys.2019.00553\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.3389\/fphys.2019.00553\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eRuan, Jun Shan et al. “Ursolic Acid Attenuates TGF-β1-Induced Epithelial-Mesenchymal Transition in NSCLC by Targeting Integrin αVβ5\/MMPs Signaling.” \u003ci\u003eOncology research\u003c\/i\u003e vol. 27,5 (2019): 593-600.\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3727%2F096504017X15051723858706\" title=\"Ruan, Jun Shan et al. “Ursolic Acid Attenuates TGF-β1-Induced Epithelial-Mesenchymal Transition in NSCLC by Targeting Integrin αVβ5\/MMPs Signaling.” Oncology research vol. 27,5 (2019): 593-600. doi:10.3727\/096504017X15051723858706\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.3727\/096504017X15051723858706\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKawashima, Keisuke et al. “CD151 confers metastatic potential to clear cell sarcoma of the soft tissue in animal model.” \u003ci\u003eOncology letters\u003c\/i\u003e vol. 17,6 (2019): 4811-4818.\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3892%2Fol.2019.10164\" title=\"Kawashima, Keisuke et al. “CD151 confers metastatic potential to clear cell sarcoma of the soft tissue in animal model.” Oncology letters vol. 17,6 (2019): 4811-4818. doi:10.3892\/ol.2019.10164\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.3892\/ol.2019.10164\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eMori, Kazunori et al. “A mitochondrial ROS pathway controls matrix metalloproteinase 9 levels and invasive properties in RAS-activated cancer cells.” \u003ci\u003eThe FEBS journal\u003c\/i\u003e vol. 286,3 (2019): 459-478.\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1111%2Ffebs.14671\" title=\"Mori, Kazunori et al. “A mitochondrial ROS pathway controls matrix metalloproteinase 9 levels and invasive properties in RAS-activated cancer cells.” The FEBS journal vol. 286,3 (2019): 459-478. doi:10.1111\/febs.14671\" rel=\"noopener noreferrer\" target=\"_blank\"\u003edoi:10.1111\/febs.14671\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRELATED PRODUCTS\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Matrix Metalloproteinases Analysis Assays Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/matrix-metalloproteinases-analysis\" rel=\"null\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eMatrix Metalloproteinases Analysis\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Extracellular Matrix Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" rel=\"null\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of QuickZyme Biosciences.\u003c\/em\u003e\u003c\/p\u003e","brand":"QuickZyme","offers":[{"title":"96 wells - Cat. No. QZBMMP9H","offer_id":40616331673752,"sku":"QZBMMP9H","price":1630.0,"currency_code":"USD","in_stock":true},{"title":"4 x 96 wells - Cat. No. QZBMMP9HX4","offer_id":44240021192916,"sku":"QZBMMP9HX4","price":4250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/MMP-9assayprinciple.png?v=1628541787"},{"product_id":"quickzyme-mouse-mmp-9-activity-assay","title":"QuickZyme Mouse MMP-9 Activity Assay","description":"\u003ch2 data-mce-fragment=\"1\"\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eQuantify both endogenous active MMP-9 and total MMP-9\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe \u003cem\u003eQuickZyme Mouse MMP-9 Activity Assay\u003c\/em\u003e enables you to specifically measure in biological samples both active mouse MMP-9, as well as (pro)MMP-9 which is activated on the plate by APMA. It can be used for the measurement of MMP-9 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine.\u003cbr\u003e\u003cbr\u003eThis 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity.\u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMeasures both endogenous active MMP-9 (naturally occurring) and total active MMP-9 (following activation with APMA).\u003c\/li\u003e\n\u003cli\u003eSamples: cell culture conditioned medium, serum, plasma, urine and tissue homogenates.\u003c\/li\u003e\n\u003cli\u003eQuantitative.\u003c\/li\u003e\n\u003cli\u003eRange: 4 – 4000 pg\/ml.\u003c\/li\u003e\n\u003cli\u003eSensitivity: 20 pg\/ml for 1 h incubation with detection reagent; 1 pg\/ml for 6 h incubation with detection reagent.\u003c\/li\u003e\n\u003cli\u003eEase-of-use: Equivalent to ELISA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eType\u003c\/h2\u003e\n\u003cp\u003eEnzyme activity immunoassay\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e\u003cimg height=\"480x480\" width=\"480x480\" alt=\"QuickZyme Mouse MMP-9 Activity Assay principle\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-9_mouse_assay_principle_480x480.png?v=1628545429\" style=\"float: none;\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-9_mouse_assay_principle_480x480.png?v=1628545429\" data-mce-style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ecell culture conditioned medium, serum, plasma, urine, tissue homogenates\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSensitivity\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e20 pg\/ml (1 hr incubation)\u003c\/li\u003e\n\u003cli\u003e1 pg\/ml (6 hr incubation)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003eQuantitative Measurement\u003c\/h2\u003e\n\u003cdiv style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-9_mouse_assay_quantitative_480x480.png?v=1628546414\" alt=\"The QuickZyme Mouse MMP-9 Activity Assay is a quantitative replacement for qualitative zymography.\" style=\"float: none;\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-9_mouse_assay_quantitative_480x480.png?v=1628546414\"\u003e\u003c\/div\u003e\n\u003cp\u003eThe QuickZyme Mouse MMP-9 Activity Assay is a quantitative replacement for qualitative zymography.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eUnopened kit: Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date.\u003c\/p\u003e\n\u003cp\u003eOpened kit \/ reconstituted reagents: Please refer to kit manual.\u003c\/p\u003e\n\u003ch2\u003eOther names\u003c\/h2\u003e\n\u003cp\u003eMatrix metalloproteinase-9, MMP9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Quickzyme Human MMP-9 Activity Assay Product Datasheet (PDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Manual-QuickZyme-human-MMP-9-activity-assay-Feb-2018.pdf?v=1628541393\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Manual-QuickZyme-human-MMP-9-activity-assay-Feb-2018.pdf?v=1628541393\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Brochure for QuickZyme Human MMP-9 Activity Assay\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Product-sheet-human-MMP-9-activity-assay-1.pdf?v=1628541393\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Product-sheet-human-MMP-9-activity-assay-1.pdf?v=1628541393\" target=\"_blank\"\u003e Product Brochure\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Frequently Asked Questions (FAQ) - QuickZyme Assays\" href=\"https:\/\/ilexlife.com\/pages\/quickzyme-biosciences-assays-frequently-asked-questions-faq\" target=\"_blank\"\u003eFrequently Asked Questions (FAQ)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAshida, Shinichi et al. “Administration of anti-inflammatory M2 macrophages suppresses progression of angiotensin II-induced aortic aneurysm in mice.” \u003ci\u003eScientific reports\u003c\/i\u003e vol. 13,1 1380. 25 Jan. 2023,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" title=\"Ashida, Shinichi et al. “Administration of anti-inflammatory M2 macrophages suppresses progression of angiotensin II-induced aortic aneurysm in mice.” Scientific reports vol. 13,1 1380. 25 Jan. 2023, doi:10.1038\/s41598-023-27412-x\" href=\"https:\/\/doi.org\/10.1038%2Fs41598-023-27412-x\" target=\"_blank\"\u003edoi:10.1038\/s41598-023-27412-x \u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eNakashima, Ryunosuke et al. “Metformin suppresses epithelial sodium channel hyperactivation and its associated phenotypes in a mouse model of obstructive lung diseases.” \u003ci\u003eJournal of pharmacological sciences\u003c\/i\u003e vol. 149,2 (2022): 37-45. \u003ca rel=\"noopener noreferrer\" title=\"Nakashima, Ryunosuke et al. “Metformin suppresses epithelial sodium channel hyperactivation and its associated phenotypes in a mouse model of obstructive lung diseases.” Journal of pharmacological sciences vol. 149,2 (2022): 37-45. doi:10.1016\/j.jphs.2022.03.002\" href=\"https:\/\/doi.org\/10.1016\/j.jphs.2022.03.002\" data-mce-href=\"https:\/\/doi.org\/10.1016\/j.jphs.2022.03.002\" target=\"_blank\"\u003edoi:10.1016\/j.jphs.2022.03.002\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eFujimoto, Yuki et al. “Dual therapy with zinc acetate and rifaximin prevents from ethanol-induced liver fibrosis by maintaining intestinal barrier integrity.” \u003ci\u003eWorld journal of gastroenterology\u003c\/i\u003e vol. 27,48 (2021): 8323-8342.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.3748%2Fwjg.v27.i48.8323\" title=\"Fujimoto, Yuki et al. “Dual therapy with zinc acetate and rifaximin prevents from ethanol-induced liver fibrosis by maintaining intestinal barrier integrity.” World journal of gastroenterology vol. 27,48 (2021): 8323-8342. doi:10.3748\/wjg.v27.i48.8323\" target=\"_blank\"\u003edoi:10.3748\/wjg.v27.i48.8323\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKuramoto, Toru et al. “Chymase as a Novel Therapeutic Target in Acute Pancreatitis.” \u003ci\u003eInternational journal of molecular sciences\u003c\/i\u003e vol. 22,22 12313. 15 Nov. 2021,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.3390%2Fijms222212313\" title=\"Kuramoto, Toru et al. “Chymase as a Novel Therapeutic Target in Acute Pancreatitis.” International journal of molecular sciences vol. 22,22 12313. 15 Nov. 2021, doi:10.3390\/ijms222212313\" target=\"_blank\"\u003edoi:10.3390\/ijms222212313\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eNikolakopoulou, Angeliki Maria et al. “Endothelial LRP1 protects against neurodegeneration by blocking cyclophilin A.” \u003ci\u003eThe Journal of experimental medicine\u003c\/i\u003e vol. 218,4 (2021): e20202207.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1084%2Fjem.20202207\" title=\"Nikolakopoulou, Angeliki Maria et al. “Endothelial LRP1 protects against neurodegeneration by blocking cyclophilin A.” The Journal of experimental medicine vol. 218,4 (2021): e20202207. doi:10.1084\/jem.20202207\" target=\"_blank\"\u003edoi:10.1084\/jem.20202207\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eShiraishi, Yasuyuki et al. “Short-term inhibition of fibrinolytic system restores locomotor function after spinal cord injury in mice.” \u003ci\u003eScientific reports\u003c\/i\u003e vol. 9,1 16024. 5 Nov. 2019,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1038%2Fs41598-019-52621-8\" title=\"Shiraishi, Yasuyuki et al. “Short-term inhibition of fibrinolytic system restores locomotor function after spinal cord injury in mice.” Scientific reports vol. 9,1 16024. 5 Nov. 2019, doi:10.1038\/s41598-019-52621-8\" target=\"_blank\"\u003edoi:10.1038\/s41598-019-52621-8\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eTakezaki, Akio et al. “A homozygous SFTPA1 mutation drives necroptosis of type II alveolar epithelial cells in patients with idiopathic pulmonary fibrosis.” \u003ci\u003eThe Journal of experimental medicine\u003c\/i\u003e vol. 216,12 (2019): 2724-2735.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1084%2Fjem.20182351\" title=\"Takezaki, Akio et al. “A homozygous SFTPA1 mutation drives necroptosis of type II alveolar epithelial cells in patients with idiopathic pulmonary fibrosis.” The Journal of experimental medicine vol. 216,12 (2019): 2724-2735. doi:10.1084\/jem.20182351\" target=\"_blank\"\u003edoi:10.1084\/jem.20182351\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKawai, Yohei et al. “Montelukast, a Cysteinyl Leukotriene Receptor 1 Antagonist, Induces M2 Macrophage Polarization and Inhibits Murine Aortic Aneurysm Formation.” \u003ci\u003eBioMed research international\u003c\/i\u003e vol. 2019 9104680. 27 May. 2019,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1155%2F2019%2F9104680\" title=\"Kawai, Yohei et al. “Montelukast, a Cysteinyl Leukotriene Receptor 1 Antagonist, Induces M2 Macrophage Polarization and Inhibits Murine Aortic Aneurysm Formation.” BioMed research international vol. 2019 9104680. 27 May. 2019, doi:10.1155\/2019\/9104680\" target=\"_blank\"\u003edoi:10.1155\/2019\/9104680\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eAndersson, Patrik et al. “Molecular mechanisms of IL-33-mediated stromal interactions in cancer metastasis.” \u003ci\u003eJCI insight\u003c\/i\u003e vol. 3,20 e122375. 18 Oct. 2018,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1172%2Fjci.insight.122375\" title=\"Andersson, Patrik et al. “Molecular mechanisms of IL-33-mediated stromal interactions in cancer metastasis.” JCI insight vol. 3,20 e122375. 18 Oct. 2018, doi:10.1172\/jci.insight.122375\" target=\"_blank\"\u003edoi:10.1172\/jci.insight.122375\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eSeifert, Robert et al. “Molecular imaging of MMP activity discriminates unstable from stable plaque phenotypes in shear-stress induced murine atherosclerosis.” \u003ci\u003ePloS one\u003c\/i\u003e vol. 13,10 e0204305. 10 Oct. 2018,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1371%2Fjournal.pone.0204305\" title=\"Seifert, Robert et al. “Molecular imaging of MMP activity discriminates unstable from stable plaque phenotypes in shear-stress induced murine atherosclerosis.” PloS one vol. 13,10 e0204305. 10 Oct. 2018, doi:10.1371\/journal.pone.0204305\" target=\"_blank\"\u003edoi:10.1371\/journal.pone.0204305\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eOrner, Caitlin A et al. “Low-Dose and Short-Duration Matrix Metalloproteinase 9 Inhibition Does Not Affect Adhesion Formation during Murine Flexor Tendon Healing.” \u003ci\u003ePlastic and reconstructive surgery\u003c\/i\u003e vol. 137,3 (2016): 545e-553e. \u003ca rel=\"noopener noreferrer\" title=\"Orner, Caitlin A et al. “Low-Dose and Short-Duration Matrix Metalloproteinase 9 Inhibition Does Not Affect Adhesion Formation during Murine Flexor Tendon Healing.” Plastic and reconstructive surgery vol. 137,3 (2016): 545e-553e. doi:10.1097\/01.prs.0000475823.01907.53\" href=\"https:\/\/doi.org\/10.1097%2F01.prs.0000475823.01907.53\" data-mce-href=\"https:\/\/doi.org\/10.1097%2F01.prs.0000475823.01907.53\" target=\"_blank\"\u003edoi:10.1097\/01.prs.0000475823.01907.53\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Matrix Metalloproteinases Analysis Assays Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/matrix-metalloproteinases-analysis\" rel=\"null\" aria-describedby=\"a11y-new-window-message\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/matrix-metalloproteinases-analysis\" target=\"_blank\"\u003eMatrix Metalloproteinases Analysis\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Extracellular Matrix Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" rel=\"null\" aria-describedby=\"a11y-new-window-message\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of QuickZyme Biosciences.\u003c\/em\u003e\u003c\/p\u003e","brand":"QuickZyme","offers":[{"title":"96 wells - Cat. No. QZBMMP9M","offer_id":40616572780696,"sku":"QZBMMP9M","price":1630.0,"currency_code":"USD","in_stock":true},{"title":"4 x 96 wells - Cat. No. QZBMMP9MX4","offer_id":44240027615444,"sku":"QZBMMP9MX4","price":4250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/MMP-9mouseassayprinciple.png?v=1628546591"},{"product_id":"quickzyme-human-mmp-14-activity-assay","title":"QuickZyme Human MMP-14 Activity Assay","description":"\u003ch2 data-mce-fragment=\"1\"\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe \u003cem\u003eQuickZyme Human MMP-14 Activity Assay\u003c\/em\u003e enables you to specifically measure active human MMP-14. It can be used for the measurement of MMP-14 activity in various biological samples, such as cultured cells and tissue homogenates.\u003cbr\u003e\u003cbr\u003eThis 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This amplification step provides a unique assay sensitivity.\u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMeasures endogenous active MMP-14 \/ MT1-MMP (naturally occurring) in complex biological samples.\u003c\/li\u003e\n\u003cli\u003eSamples: cell extracts, tissue homogenates, tissue extracts.\u003c\/li\u003e\n\u003cli\u003eQuantitative.\u003c\/li\u003e\n\u003cli\u003eRange: 0.1 to 16 ng\/ml\u003c\/li\u003e\n\u003cli\u003eSensitivity: 0.5 ng\/ml (2 h incubation with detection reagent); 0.1 ng\/ml (5 h incubation with detection reagent).\u003c\/li\u003e\n\u003cli\u003eEase-of-use: Equivalent to ELISA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eType\u003c\/h2\u003e\n\u003cp\u003eEnzyme activity immunoassay\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e\u003cimg alt=\"QuickZyme Human MMP-14 Activity Assay principle\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-14_activity_assay_principle.png?v=1628604474\" width=\"480x480\" height=\"480x480\" style=\"float: none;\" data-mce-style=\"float: none;\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-14_activity_assay_principle.png?v=1628604474\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ecell extracts, tissue homogenates, tissue extracts\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSensitivity\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e0.5 ng\/ml (2 h incubation)\u003c\/li\u003e\n\u003cli\u003e0.1 ng\/ml (5 h incubation)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003eQuantitative Measurement\u003c\/h2\u003e\n\u003cdiv style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e\u003cimg alt=\"Typical data with 2 hours incubation: QuickZyme Human MMP-14 Activity Assay\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-14_typical_data_480x480.png?v=1628604568\" width=\"480x480\" height=\"480x480\" style=\"float: none;\" data-mce-style=\"float: none;\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MMP-14_typical_data_480x480.png?v=1628604568\"\u003e\u003c\/div\u003e\n\u003cp\u003eTypical data with 2 hours incubation. The shown data curve is provided for demonstration only.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eUnopened kit: Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date.\u003c\/p\u003e\n\u003cp\u003eOpened kit \/ reconstituted reagents: Please refer to kit manual.\u003c\/p\u003e\n\u003ch2\u003eOther names\u003c\/h2\u003e\n\u003cp\u003eMatrix metalloproteinase-14, MMP14, MMP-X1, Membrane-type matrix metalloproteinase 1, MT-MMP 1, MTMMP1, Membrane-type-1 matrix metalloproteinase, MT1-MMP, MT1MMP\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Quickzyme Human MMP-14 Activity Assay Manual Product Datasheet (PDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Manual-QuickZyme-human-MMP-14-activity-assay-Feb-2018.pdf?v=1628604777\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Manual-QuickZyme-human-MMP-14-activity-assay-Feb-2018.pdf?v=1628604777\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Brochure for QuickZyme Human MMP-14 Activity Assay\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Product-sheet-human-MMP-14-activity-assay-August-2016-1.pdf?v=1628604777\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Product-sheet-human-MMP-14-activity-assay-August-2016-1.pdf?v=1628604777\" target=\"_blank\"\u003eProduct Brochure\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Frequently Asked Questions (FAQ) - QuickZyme Assays\" href=\"https:\/\/ilexlife.com\/pages\/quickzyme-biosciences-assays-frequently-asked-questions-faq\" target=\"_blank\"\u003eFrequently Asked Questions (FAQ)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/matrix-metalloproteinases-analysis\" title=\"Matrix Metalloproteinases Analysis Assays Product Collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/matrix-metalloproteinases-analysis\" target=\"_blank\"\u003eMatrix Metalloproteinases Analysis\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" title=\"Extracellular Matrix Assays product collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an official distributor of QuickZyme Biosciences.\u003c\/em\u003e\u003c\/p\u003e","brand":"QuickZyme","offers":[{"title":"96 wells - Cat. No. QZBMMP14H","offer_id":40623253061784,"sku":"QZBMMP14H","price":1630.0,"currency_code":"USD","in_stock":true},{"title":"4 x 96 wells - Cat. No. QZBMMP14HX4","offer_id":44239872295124,"sku":"QZBMMP14HX4","price":4250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/MMP-14activityassayprinciple.png?v=1628604713"},{"product_id":"quickzyme-soluble-collagen-assay","title":"QuickZyme Soluble Collagen Assay (96-well plate format)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003eQuickZyme Soluble Collagen Assay\u003c\/strong\u003e kit is a quantitative, dye-binding method for the measurement of all types of fibrillar collagen, in a fast and simple 96-well plate format.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eType\u003c\/h2\u003e\n\u003cp\u003eColorimetric Assay (Sirius Red Collagen Assay)\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThe QuickZyme Soluble Collagen Assay is based on binding of Sirius Red dye to collagen. Following binding of the dye, the collagen-dye complex precipitates, resulting in a colored pellet. This color can be released in an alkaline solution and then read with a microplate reader.\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg height=\"480x480\" width=\"480x480\" alt=\"QuickZyme Soluble Collagen Assay principle\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/QuickZyme_soluble_collagen_assay_principle.png?v=1628623386\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eType of Collagen Measured:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMeasures all types of fibrillar collagen, species independent. Measures soluble or solubilized collagens.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAssay Range:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.5 - 10 μg\/ml \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.69 ng\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFormat:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e96-well plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSample Volume:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 -140 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Types:\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003econditioned cell culture media\u003c\/li\u003e\n\u003cli\u003ecellular extracts\u003c\/li\u003e\n\u003cli\u003esolubilized collagen fractions\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Run-Time:\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 3 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003eQuickZyme Biosciences\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eCountry of Origin:\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003eThe Netherlands\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cp\u003eCollagen is one of the main components of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Therefore quantitative measurement of collagen production is important in many disease related studies.\u003cbr\u003e\u003cbr\u003eThe QuickZyme Soluble Collagen Assay measures various types of fibrillar collagen. The most abundant collagen types are collagen type I (skin, bone, vessels, connective tissue), type II (cartilage) and type III (skin, bone, connective tissue).\u003cbr\u003e\u003cbr\u003eThis assay is specific for collagen. After heating at 50°C the signal is lost, since the assay does not recognize denatured collagen (gelatin). The assay can be used to analyze soluble collagen or collagen that is solubilized by acetic acid alone or in combination with pepsin. The assay is intended for collagen analysis in conditioned cell culture media and cellular extracts. \u003cstrong\u003eThe assay is not affected by serum up to at least 10%.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/QuickZyme_soluble_collagen_standard_curve.png?v=1628623512\" style=\"float: none;\" alt=\"QuickZyme Soluble Collagen Assay: Typical collagen standard curve in the range of 0.5 – 10 μg collagen\/well (3.5 - 70 μg\/ml; blank value 0.070)\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eTypical collagen standard curve in the range of 0.5 – 10 μg collagen\/well (3.5 - 70 μg\/ml; blank value 0.070).\u003c\/em\u003e\u003c\/p\u003e\n\u003ch2\u003eTutorial Video\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003ciframe height=\"315\" width=\"560\" title=\"YouTube video player\" src=\"https:\/\/www.youtube.com\/embed\/wUZZT_L4FQk\"\u003e\u003c\/iframe\u003e\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eUnopened kit:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eStore at 4°C. Do not use kit components past kit expiration date.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eOpened kit \/ reconstituted reagents:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCollagen standard dissolved in 20 mM HAc: store at 4°C up to 1 month\u003c\/li\u003e\n\u003cli\u003eDye solution: store at 4°C up to 1 month\u003c\/li\u003e\n\u003cli\u003eDilution buffer: store at -20°C up to 6 months\u003c\/li\u003e\n\u003cli\u003eWash buffer: store at -20°C up to 6 months\u003c\/li\u003e\n\u003cli\u003eDetection solution: store either at 4°C or room temperature up to 1 month\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Manual-QuickZyme-Soluble-Collagen-assay-Feb-2018.pdf?v=1628623726\" title=\"Product Datasheet (PDS): QuickZyme Soluble Collagen Assay Manual\/Protocol\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Frequently Asked Questions (FAQ) - QuickZyme Assays\" href=\"https:\/\/ilexlife.com\/pages\/quickzyme-biosciences-assays-frequently-asked-questions-faq\" target=\"_blank\"\u003eFrequently Asked Questions (FAQ)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eApplication note 1: \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Application-note-collagen-assays-A4.pdf?v=1628014552\" title=\"Collagen analysis: Which assay can be used for what application?\" target=\"_blank\"\u003eHow to choose your collagen assay?\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eApplication note 2: \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/application-note-collagen-measurements-in-mouse-tissues-A4.pdf?v=1628014696\" title=\"Collagen analysis in mouse tissues using various assays\" target=\"_blank\"\u003eHow to measure collagen in mouse tissues?\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eApplication note 3: \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/application-note-protein-assay-applications-1.pdf?v=1628014843\" title=\"Collagen per protein analysis in fresh, frozen and formalin-fixed tissues\" target=\"_blank\"\u003eCollagen per protein analysis in fresh, frozen and formalin-fixed tissues\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eKondo, Masahiro et al. “Dersimelagon, a novel oral melanocortin 1 receptor agonist, demonstrates disease-modifying effects in preclinical models of systemic sclerosis.” \u003ci\u003eArthritis research \u0026amp; therapy\u003c\/i\u003e vol. 24,1 210. 1 Sep. 2022,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1186%2Fs13075-022-02899-3\" title=\"Kondo, Masahiro et al. “Dersimelagon, a novel oral melanocortin 1 receptor agonist, demonstrates disease-modifying effects in preclinical models of systemic sclerosis.” Arthritis research \u0026amp; therapy vol. 24,1 210. 1 Sep. 2022, doi:10.1186\/s13075-022-02899-3\" target=\"_blank\"\u003edoi:10.1186\/s13075-022-02899-3\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKiseleva, Ekaterina et al. “Interaction between mesenchymal stem cells and myoblasts in the context of facioscapulohumeral muscular dystrophy contributes to the disease phenotype.” \u003ci\u003eJournal of cellular physiology\u003c\/i\u003e vol. 237,8 (2022): 3328-3337.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1002%2Fjcp.30789\" title=\"Kiseleva, Ekaterina et al. “Interaction between mesenchymal stem cells and myoblasts in the context of facioscapulohumeral muscular dystrophy contributes to the disease phenotype.” Journal of cellular physiology vol. 237,8 (2022): 3328-3337. doi:10.1002\/jcp.30789\" target=\"_blank\"\u003edoi:10.1002\/jcp.30789\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eQuesnel, Katherine et al. “CCN1 expression by fibroblasts is required for bleomycin-induced skin fibrosis.” \u003ci\u003eMatrix biology plus\u003c\/i\u003e vol. 3 100009. 6 Jul. 2019,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1016%2Fj.mbplus.2019.100009\" title=\"Quesnel, Katherine et al. “CCN1 expression by fibroblasts is required for bleomycin-induced skin fibrosis.” Matrix biology plus vol. 3 100009. 6 Jul. 2019, doi:10.1016\/j.mbplus.2019.100009\" target=\"_blank\"\u003edoi:10.1016\/j.mbplus.2019.100009\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKano, Miyu et al. “Attenuation of murine sclerodermatous models by the selective S1P\u003csub\u003e1\u003c\/sub\u003e receptor modulator cenerimod.” \u003ci\u003eScientific reports\u003c\/i\u003e vol. 9,1 658. 24 Jan. 2019,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1038%2Fs41598-018-37074-9\" title=\"Kano, Miyu et al. “Attenuation of murine sclerodermatous models by the selective S1P1 receptor modulator cenerimod.” Scientific reports vol. 9,1 658. 24 Jan. 2019, doi:10.1038\/s41598-018-37074-9\" target=\"_blank\"\u003edoi:10.1038\/s41598-018-37074-9\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eMatsushita, Takashi et al. “BAFF inhibition attenuates fibrosis in scleroderma by modulating the regulatory and effector B cell balance.” \u003ci\u003eScience advances\u003c\/i\u003e vol. 4,7 eaas9944. 11 Jul. 2018,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1126%2Fsciadv.aas9944\" title=\"Matsushita, Takashi et al. “BAFF inhibition attenuates fibrosis in scleroderma by modulating the regulatory and effector B cell balance.” Science advances vol. 4,7 eaas9944. 11 Jul. 2018, doi:10.1126\/sciadv.aas9944\" target=\"_blank\"\u003edoi:10.1126\/sciadv.aas9944\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eHan, Zonglin et al. “Inhibition of prostaglandin E2 protects abdominal aortic aneurysm from expansion through regulating miR-29b-mediated fibrotic ECM expression.” \u003ci\u003eExperimental and therapeutic medicine\u003c\/i\u003e vol. 16,1 (2018): 155-160.\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.3892%2Fetm.2018.6160\" title=\"Han, Zonglin et al. “Inhibition of prostaglandin E2 protects abdominal aortic aneurysm from expansion through regulating miR-29b-mediated fibrotic ECM expression.” Experimental and therapeutic medicine vol. 16,1 (2018): 155-160. doi:10.3892\/etm.2018.6160\" target=\"_blank\"\u003edoi:10.3892\/etm.2018.6160\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eGoldstein, Benjamin D et al. “Chronic asthma and Mesenchymal stem cells: Hyaluronan and airway remodeling.” \u003ci\u003eJournal of inflammation (London, England)\u003c\/i\u003e vol. 14 18. 30 Aug. 2017,\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"noopener noreferrer\" href=\"https:\/\/doi.org\/10.1186%2Fs12950-017-0165-4\" title=\"Goldstein, Benjamin D et al. “Chronic asthma and Mesenchymal stem cells: Hyaluronan and airway remodeling.” Journal of inflammation (London, England) vol. 14 18. 30 Aug. 2017, doi:10.1186\/s12950-017-0165-4\" target=\"_blank\"\u003edoi:10.1186\/s12950-017-0165-4\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"null\" href=\"https:\/\/ilexlife.com\/collections\/assays-for-collagen-analysis\" title=\"Collagen and Hydroxyproline Assays product collection\" target=\"_blank\"\u003eCollagen and Hydroxyproline Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" title=\"Browse Extracellular Matrix Assays product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-assays\" target=\"_blank\"\u003eExtracellular Matrix Assays\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"null\" title=\"Extracellular Matrix Molecules product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" target=\"_blank\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003cem\u003eIlex Life Sciences LLC is an official distributor of QuickZyme Biosciences.\u003c\/em\u003e\u003c\/p\u003e","brand":"QuickZyme","offers":[{"title":"QuickZyme Soluble Collagen Assay, 1 plate (96 wells) - Cat. No. QZBCOL1-1","offer_id":40624605855896,"sku":"QZBCOL1-1","price":599.0,"currency_code":"USD","in_stock":true},{"title":"QuickZyme Soluble Collagen Assay, 2 plates (2 x 96 wells) - Cat. No. QZBCOL1-2","offer_id":40624605888664,"sku":"QZBCOL1-2","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"QuickZyme Soluble Collagen Assay, 5 plates (5 x 96 wells) - Cat. No. QZBCOL1-5","offer_id":40624605921432,"sku":"QZBCOL1-5","price":2230.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/IMG_2984.png?v=1778176908"},{"product_id":"collagen-i-bovine-skin","title":"Collagen-I Protein, Bovine Skin","description":"\u003ch2\u003e\u003cspan data-mce-fragment=\"1\"\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eNOTE: Higher-purity Type I Collagen is now available at a lower cost here: \u003ca href=\"https:\/\/ilexlife.com\/collections\/promed-bioscience-collagen\" title=\"Promed Bioscience High-Purity Collagen Biomaterials Product Collection\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/promed-bioscience-collagen\"\u003ePromed Bioscience High-Purity Collagen\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eBovine Collagen-I is a natural protein purified from bovine skin. Collagen-I is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePurified Type I Collagen is used in many research applications such as cell culture surface coatings, tissue engineering scaffolds, 3D bioprinting, and cell functional assays.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eCollagen, a major component of the extracellular matrix, is a fibrous protein that provides tensile strength to tissues giving them structural integrity. Collagens contribute to mechanical properties, organization, and shape of tissues. Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eType I Collagen is by far the most abundant protein in all vertebrates. It assembles into fibers that form the structural and mechanical scaffold (matrix) of bone, skin, tendons, cornea, blood vessel walls and other connective tissues. Type I collagen consists of two α1(I) chains and one α2(I) chain.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cp\u003e\u003cspan mce-data-marked=\"1\"\u003eType 1 Collagen, Type I Collagen, Collagen-1\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eBovine skin.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eFiltered white lyophilized (freeze-dried) powder. Collagen-I was lyophilized without additives.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003ePurity \u0026gt;\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003cspan\u003e \u003c\/span\u003e90.0%.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eWe recommend to add 0.1M acetic acid pH 3.0 to prepare a working stock solution of approximately 1-5 mg\/ml and let the lyophilized pellet dissolve completely.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature.  Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eLyophilized Collagen-I, although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution, Collagen-I should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% \u003ca href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" title=\"HSA \/ BSA PRODUCT COLLECTION\" target=\"_blank\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Collagen-I Protein, Bovine Skin, Product Datasheet (PDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A12680_Collagen-I_Bovine.pdf?v=1629472489\" target=\"_blank\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A12680_Collagen-I_Bovine.pdf?v=1629472489\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWant to learn more about collagen? We have compiled the links below that contain information which you may find interesting:\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Ricard-Blum, Sylvie. “The collagen family.” Cold Spring Harbor perspectives in biology vol. 3,1 a004978. 1 Jan. 2011, doi:10.1101\/cshperspect.a004978\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3003457\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3003457\/\"\u003e\u003cspan\u003eThe Collagen Family\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Mathew-Steiner, Shomita S et al. “Collagen in Wound Healing.” Bioengineering (Basel, Switzerland) vol. 8,5 63. 11 May. 2021, doi:10.3390\/bioengineering8050063\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8151502\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8151502\/\"\u003e\u003cspan\u003eCollagen In Wound Healing\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Musiime, Moses et al. “Collagen Assembly at the Cell Surface: Dogmas Revisited.” Cells vol. 10,3 662. 16 Mar. 2021, doi:10.3390\/cells10030662\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8002325\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8002325\/\"\u003eCollagen Assembly at the Cell Surface: Dogmas Revisited\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Benwood, Claire et al. “Natural Biomaterials and Their Use as Bioinks for Printing Tissues.” Bioengineering (Basel, Switzerland) vol. 8,2 27. 20 Feb. 2021, doi:10.3390\/bioengineering8020027\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7924193\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7924193\/\"\u003eNatural Biomaterials and Their Use as Bioinks for Printing Tissues\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Chan, Weng Wan et al. “Additive Biomanufacturing with Collagen Inks.” Bioengineering (Basel, Switzerland) vol. 7,3 66. 1 Jul. 2020, doi:10.3390\/bioengineering7030066\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7552643\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7552643\/\"\u003eAdditive Biomanufacturing with Collagen Inks\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Xu, Yujia, and Michele Kirchner. “Collagen Mimetic Peptides.” Bioengineering (Basel, Switzerland) vol. 8,1 5. 5 Jan. 2021, doi:10.3390\/bioengineering8010005\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7824840\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7824840\/\"\u003eCollagen Mimetic Peptides\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Xu, Shuaishuai et al. “The role of collagen in cancer: from bench to bedside.” Journal of translational medicine vol. 17,1 309. 14 Sep. 2019, doi:10.1186\/s12967-019-2058-1\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6744664\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6744664\/\"\u003eThe role of collagen in cancer: from bench to bedside\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6410095\/\" title=\"Rahman, M Azizur. “Collagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications.” Marine drugs vol. 17,2 118. 14 Feb. 2019, doi:10.3390\/md17020118\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6410095\/\" target=\"_blank\"\u003eCollagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/promed-bioscience-collagen\" title=\"Promed Bioscience High-Purity Collagen Biomaterials Product Collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/promed-bioscience-collagen\"\u003ePromed Bioscience High-Purity Collagen\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" title=\"Attachment Factors Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/attachment-factors-cell-culture\" target=\"_blank\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/attachment-factors-cell-culture\"\u003eAttachment Factors\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" title=\"Extracellular Matrix Molecules product collection\" target=\"_blank\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"250 µg - Cat. No. A12680250","offer_id":40734136631448,"sku":"A12680250","price":210.0,"currency_code":"USD","in_stock":true},{"title":"500 µg - Cat. No. A12680500","offer_id":40734136664216,"sku":"A12680500","price":265.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A126801MG","offer_id":40734302109848,"sku":"A126801MG","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/IlexLifeSciencesCollagen-I_TypeICollagen.png?v=1691007220"},{"product_id":"collagen-i-protein-goat-tissue","title":"Collagen-I Protein, Goat Tissue","description":"\u003ch2\u003e\u003cspan data-mce-fragment=\"1\"\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eNOTE: Higher-purity Type I Collagen is now available at a lower cost here: \u003ca data-mce-fragment=\"1\" href=\"https:\/\/ilexlife.com\/collections\/promed-bioscience-collagen\" title=\"Promed Bioscience High-Purity Collagen Biomaterials Product Collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/promed-bioscience-collagen\"\u003ePromed Bioscience High-Purity Collagen\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGoat Collagen-I is a natural protein purified from Goat tissues. Collagen-I is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePurified Type I Collagen is used in many research applications such as cell culture surface coatings, tissue engineering scaffolds, 3D bioprinting, and cell functional assays.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eCollagen, a major component of the extracellular matrix, is a fibrous protein that provides tensile strength to tissues giving them structural integrity. Collagens contribute to mechanical properties, organization, and shape of tissues. Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eType I Collagen is by far the most abundant protein in all vertebrates. It assembles into fibers that form the structural and mechanical scaffold (matrix) of bone, skin, tendons, cornea, blood vessel walls and other connective tissues. Type I collagen consists of two α1(I) chains and one α2(I) chain.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cp\u003e\u003cspan mce-data-marked=\"1\"\u003eType 1 Collagen, Type I Collagen, Collagen-1\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eGoat tissues.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eFiltered white lyophilized (freeze-dried) powder. Collagen-I was lyophilized without additives.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003ePurity \u0026gt;\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003cspan\u003e \u003c\/span\u003e90.0%.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eWe recommend to add 0.5 M acetic acid, pH 2.5 to prepare a working stock solution not less than 100 µg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature.  Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eLyophilized Collagen-I although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Collagen-I should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% \u003ca title=\"HSA \/ BSA PRODUCT COLLECTION\" href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" target=\"_blank\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca title=\"Collagen-I Protein, Goat Tissue Product Datasheet (PDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A12682_Collagen-I_Goat.pdf?v=1629755595\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWant to learn more about collagen? We have compiled the links below that contain information which you may find interesting:\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"null\" aria-describedby=\"a11y-new-window-external-message\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3003457\/\" title=\"Ricard-Blum, Sylvie. “The collagen family.” Cold Spring Harbor perspectives in biology vol. 3,1 a004978. 1 Jan. 2011, doi:10.1101\/cshperspect.a004978\" target=\"_blank\"\u003e\u003cspan\u003eThe Collagen Family\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"null\" aria-describedby=\"a11y-new-window-external-message\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8151502\/\" title=\"Mathew-Steiner, Shomita S et al. “Collagen in Wound Healing.” Bioengineering (Basel, Switzerland) vol. 8,5 63. 11 May. 2021, doi:10.3390\/bioengineering8050063\" target=\"_blank\"\u003e\u003cspan\u003eCollagen In Wound Healing\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"null\" aria-describedby=\"a11y-new-window-external-message\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8002325\/\" title=\"Musiime, Moses et al. “Collagen Assembly at the Cell Surface: Dogmas Revisited.” Cells vol. 10,3 662. 16 Mar. 2021, doi:10.3390\/cells10030662\" target=\"_blank\"\u003eCollagen Assembly at the Cell Surface: Dogmas Revisited\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"null\" aria-describedby=\"a11y-new-window-external-message\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7924193\/\" title=\"Benwood, Claire et al. “Natural Biomaterials and Their Use as Bioinks for Printing Tissues.” Bioengineering (Basel, Switzerland) vol. 8,2 27. 20 Feb. 2021, doi:10.3390\/bioengineering8020027\" target=\"_blank\"\u003eNatural Biomaterials and Their Use as Bioinks for Printing Tissues\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"null\" aria-describedby=\"a11y-new-window-external-message\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7552643\/\" title=\"Chan, Weng Wan et al. “Additive Biomanufacturing with Collagen Inks.” Bioengineering (Basel, Switzerland) vol. 7,3 66. 1 Jul. 2020, doi:10.3390\/bioengineering7030066\" target=\"_blank\"\u003eAdditive Biomanufacturing with Collagen Inks\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"null\" aria-describedby=\"a11y-new-window-external-message\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7824840\/\" title=\"Xu, Yujia, and Michele Kirchner. “Collagen Mimetic Peptides.” Bioengineering (Basel, Switzerland) vol. 8,1 5. 5 Jan. 2021, doi:10.3390\/bioengineering8010005\" target=\"_blank\"\u003eCollagen Mimetic Peptides\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"null\" aria-describedby=\"a11y-new-window-external-message\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6744664\/\" title=\"Xu, Shuaishuai et al. “The role of collagen in cancer: from bench to bedside.” Journal of translational medicine vol. 17,1 309. 14 Sep. 2019, doi:10.1186\/s12967-019-2058-1\" target=\"_blank\"\u003eThe role of collagen in cancer: from bench to bedside\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"null\" aria-describedby=\"a11y-new-window-external-message\" title=\"Rahman, M Azizur. “Collagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications.” Marine drugs vol. 17,2 118. 14 Feb. 2019, doi:10.3390\/md17020118\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6410095\/\" target=\"_blank\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6410095\/\"\u003eCollagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/promed-bioscience-collagen\" title=\"Promed Bioscience High-Purity Collagen Biomaterials Product Collection\" data-mce-href=\"https:\/\/ilexlife.com\/collections\/promed-bioscience-collagen\"\u003ePromed Bioscience High-Purity Collagen\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" title=\"Attachment Factors Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/attachment-factors-cell-culture\" target=\"_blank\"\u003eAttachment Factors\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" title=\"Extracellular Matrix Molecules product collection\" target=\"_blank\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"250 µg - Cat. No. A12682250","offer_id":40767564349592,"sku":"A12682250","price":210.0,"currency_code":"USD","in_stock":true},{"title":"500 µg - Cat. No. A12682500","offer_id":40767564382360,"sku":"A12682500","price":265.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A126821MG","offer_id":40767564415128,"sku":"A126821MG","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/IlexLifeSciencesCollagen-I_TypeICollagen_5641ab83-bca8-4741-8f46-2368d45b3770.png?v=1691007279"},{"product_id":"collagen-i-protein-mouse-tail-tendon","title":"Collagen-I Protein, Mouse Tail Tendon","description":"\u003ch2\u003e\u003cspan data-mce-fragment=\"1\"\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong\u003eNOTE: Higher-purity Type I Collagen is now available at a lower cost here: \u003ca href=\"https:\/\/ilexlife.com\/collections\/promed-bioscience-collagen\" title=\"Promed Bioscience High-Purity Collagen Biomaterials Product Collection\" data-mce-fragment=\"1\"\u003ePromed Bioscience High-Purity Collagen\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eMouse Collagen-I is a natural protein purified from Mouse tail tendon. Collagen-I is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePurified Type I Collagen is used in many research applications such as cell culture surface coatings, tissue engineering scaffolds, 3D bioprinting, and cell functional assays.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eCollagen, a major component of the extracellular matrix, is a fibrous protein that provides tensile strength to tissues giving them structural integrity. Collagens contribute to mechanical properties, organization, and shape of tissues. Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eType I Collagen is by far the most abundant protein in all vertebrates. It assembles into fibers that form the structural and mechanical scaffold (matrix) of bone, skin, tendons, cornea, blood vessel walls and other connective tissues. Type I collagen consists of two α1(I) chains and one α2(I) chain.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMurine Type 1 Collagen, Type I Collagen, Collagen-1\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eMouse tail tendon.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eFiltered white lyophilized (freeze-dried) powder. Collagen-I was lyophilized without additives.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003ePurity \u0026gt;\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003cspan\u003e \u003c\/span\u003e90.0%.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eWe recommend to add 0.5 M acetic acid, pH 3 at 4°C to prepare a working stock solution not less than 100 µg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eShips at ambient temperature. Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eLyophilized Collagen-I, although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution, Collagen-I should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% \u003ca title=\"HSA \/ BSA PRODUCT COLLECTION\" href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" target=\"_blank\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A12681_Collagen-I_Mouse_2024.02.pdf\" title=\"Collagen-I Protein, Mouse Tail Tendon Product Datasheet (PDS)\" target=\"_blank\"\u003e Product Datasheet (PDS)\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Promed Bioscience High-Purity Collagen Biomaterials Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/promed-bioscience-collagen\"\u003ePromed Bioscience High-Purity Collagen\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" title=\"Attachment Factors Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/attachment-factors-cell-culture\" target=\"_blank\"\u003eAttachment Factors\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" title=\"Extracellular Matrix Molecules product collection\" target=\"_blank\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"250 µg - Cat. No. A12681250","offer_id":46372022780116,"sku":"A12681250","price":285.0,"currency_code":"USD","in_stock":true},{"title":"500 µg - Cat. No. A12681500","offer_id":46372023304404,"sku":"A12681250","price":340.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A126811MG","offer_id":40767631261848,"sku":"A126811MG","price":575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/CollagenI-mouse.png?v=1689780175"},{"product_id":"collagen-ii-protein-bovine-knee-joint","title":"Collagen-II Protein, Bovine Knee Joint","description":"\u003ch2\u003e\u003cspan data-mce-fragment=\"1\"\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBovine Collagen-II is a natural protein purified from bovine knee joint. Collagen-II is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCollagen, a major component of the extracellular matrix, is a fibrous protein that provides tensile strength to tissues giving them structural integrity. Collagens contribute to mechanical properties, organization, and shape of tissues. Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eType II collagen is one of the major ECM components of articular cartilage. Type II collagen \u003cspan data-mce-fragment=\"1\"\u003eis a fibril-forming collagen that forms the bulk of the vitreous collagen fibrils, accounting for 60–75% of the collagen within the fibrils. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eBovine knee joint.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eFiltered white lyophilized (freeze-dried) powder. Collagen-II was lyophilized without additives.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003ePurity \u0026gt;\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003cspan\u003e \u003c\/span\u003e95.0%.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eWe recommend to add 50 mM acetic acid to prepare a working stock solution of approximately 1-5 mg\/ml and let the lyophilized pellet dissolve completely.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature.  Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eLyophilized Collagen-II, although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution, Collagen-II should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% \u003ca href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" target=\"_blank\" title=\"HSA \/ BSA PRODUCT COLLECTION\" rel=\"noopener noreferrer\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cp\u003e\u003cspan mce-data-marked=\"1\"\u003eType 2 Collagen, Type II Collagen, Collagen-2, COL2A1, Collagen alpha-1(II) chain\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eRESOURCES\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A12679_Collagen-II_Bovine.pdf?v=1629925390\" title=\"Collagen-II Protein, Bovine Knee Joint, Product Datasheet (PDS)\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWant to learn more about collagen? We have compiled the links below that contain information which you may find interesting:\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"UniProt Knowledgebase: UniProtKB - P02459 (CO2A1_BOVIN): Collagen alpha-1(II) chain\" href=\"https:\/\/www.uniprot.org\/uniprot\/P02459\" target=\"_blank\" data-mce-href=\"https:\/\/www.uniprot.org\/uniprot\/P02459\"\u003eUniProt Knowledgebase: UniProtKB - P02459 (CO2A1_BOVIN): \u003cspan data-mce-fragment=\"1\"\u003eCollagen alpha-1(II) chain\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/pubchem.ncbi.nlm.nih.gov\/gene\/407142\" title=\"PubChem Gene Summary: COL2A1 - collagen type II alpha 1 chain (cattle)\" target=\"_blank\"\u003e\u003cspan data-mce-fragment=\"1\"\u003ePubChem Gene Summary: COL2A1 - collagen type II alpha 1 chain (cattle)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Ricard-Blum, Sylvie. “The collagen family.” Cold Spring Harbor perspectives in biology vol. 3,1 a004978. 1 Jan. 2011, doi:10.1101\/cshperspect.a004978\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3003457\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003e\u003cspan\u003eThe Collagen Family\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Mathew-Steiner, Shomita S et al. “Collagen in Wound Healing.” Bioengineering (Basel, Switzerland) vol. 8,5 63. 11 May. 2021, doi:10.3390\/bioengineering8050063\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8151502\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003e\u003cspan\u003eCollagen In Wound Healing\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Musiime, Moses et al. “Collagen Assembly at the Cell Surface: Dogmas Revisited.” Cells vol. 10,3 662. 16 Mar. 2021, doi:10.3390\/cells10030662\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8002325\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eCollagen Assembly at the Cell Surface: Dogmas Revisited\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Benwood, Claire et al. “Natural Biomaterials and Their Use as Bioinks for Printing Tissues.” Bioengineering (Basel, Switzerland) vol. 8,2 27. 20 Feb. 2021, doi:10.3390\/bioengineering8020027\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7924193\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eNatural Biomaterials and Their Use as Bioinks for Printing Tissues\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Chan, Weng Wan et al. “Additive Biomanufacturing with Collagen Inks.” Bioengineering (Basel, Switzerland) vol. 7,3 66. 1 Jul. 2020, doi:10.3390\/bioengineering7030066\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7552643\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eAdditive Biomanufacturing with Collagen Inks\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Xu, Yujia, and Michele Kirchner. “Collagen Mimetic Peptides.” Bioengineering (Basel, Switzerland) vol. 8,1 5. 5 Jan. 2021, doi:10.3390\/bioengineering8010005\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7824840\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eCollagen Mimetic Peptides\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Xu, Shuaishuai et al. “The role of collagen in cancer: from bench to bedside.” Journal of translational medicine vol. 17,1 309. 14 Sep. 2019, doi:10.1186\/s12967-019-2058-1\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6744664\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eThe role of collagen in cancer: from bench to bedside\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6410095\/\" title=\"Rahman, M Azizur. “Collagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications.” Marine drugs vol. 17,2 118. 14 Feb. 2019, doi:10.3390\/md17020118\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6410095\/\" target=\"_blank\"\u003eCollagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRELATED PRODUCTS\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/attachment-factors-cell-culture\" title=\"Attachment Factors Product Collection\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eAttachment Factors\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Extracellular Matrix Molecules product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" target=\"_blank\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"250 µg - Cat. No. A12679250","offer_id":40782888861848,"sku":"A12679250","price":210.0,"currency_code":"USD","in_stock":true},{"title":"500 µg - Cat. No. A12679500","offer_id":40782888894616,"sku":"A12679500","price":265.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A126791MG","offer_id":40782888927384,"sku":"A126791MG","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A12679_COL-IIBOVINE.jpg?v=1691007086"},{"product_id":"collagen-iv-protein-bovine-placenta","title":"Collagen-IV Protein, Bovine Placenta","description":"\u003ch2\u003e\u003cspan data-mce-fragment=\"1\"\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBovine Collagen-IV is a natural protein purified from bovine placenta. Collagen-IV is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCollagen, a major component of the extracellular matrix, is a fibrous protein that provides tensile strength to tissues giving them structural integrity. Collagens contribute to mechanical properties, organization, and shape of tissues. Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eType IV collagen is a major component of the basement membrane, which is the specialized sheet-like ECM of multicellular tissues that exists around certain cell types (e.g., skeletal muscle cells, smooth muscle cells, heart muscle cells, and adipocytes).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eBovine placenta.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eFiltered white lyophilized (freeze-dried) powder. Collagen-IV was lyophilized without additives.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003ePurity \u0026gt;\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003cspan\u003e \u003c\/span\u003e90.0%.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eWe recommend to reconstitute the lyophilized Collagen-IV in 20 mM acetic acid not less than 1 mg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature.  Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eLyophilized Collagen-IV, although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution, Collagen-IV should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% \u003ca href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" target=\"_blank\" title=\"HSA \/ BSA PRODUCT COLLECTION\" rel=\"noopener noreferrer\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e.  Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cp\u003e\u003cspan mce-data-marked=\"1\"\u003eType IV Collagen, Type 4 Collagen, Collagen-4, COL4A1, Collagen alpha-1(IV) chain\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eRESOURCES\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A12678_Collagen-IV_Bovine.pdf?v=1629926839\" title=\"Collagen-IV Protein, Bovine Placenta, Product Datasheet (PDS)\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWant to learn more about collagen? We have compiled the links below that contain information which you may find interesting:\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/Q7SIB2\" title=\"UniProt Knowledgebase: UniProtKB - Q7SIB2 (CO4A1_BOVIN): Collagen alpha-1(IV) chain\" target=\"_blank\"\u003eUniProt Knowledgebase: UniProtKB - Q7SIB2 (CO4A1_BOVIN): Collagen alpha-1(IV) chain\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/pubchem.ncbi.nlm.nih.gov\/gene\/282191\" title=\"PubChem Gene Summary: COL4A1 - collagen type IV alpha 1 chain (cattle)\" target=\"_blank\"\u003e\u003cspan data-mce-fragment=\"1\"\u003ePubChem Gene Summary: COL4A1 - collagen type IV alpha 1 chain (cattle)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Ricard-Blum, Sylvie. “The collagen family.” Cold Spring Harbor perspectives in biology vol. 3,1 a004978. 1 Jan. 2011, doi:10.1101\/cshperspect.a004978\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3003457\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003e\u003cspan\u003eThe Collagen Family\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Mathew-Steiner, Shomita S et al. “Collagen in Wound Healing.” Bioengineering (Basel, Switzerland) vol. 8,5 63. 11 May. 2021, doi:10.3390\/bioengineering8050063\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8151502\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003e\u003cspan\u003eCollagen In Wound Healing\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Musiime, Moses et al. “Collagen Assembly at the Cell Surface: Dogmas Revisited.” Cells vol. 10,3 662. 16 Mar. 2021, doi:10.3390\/cells10030662\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8002325\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eCollagen Assembly at the Cell Surface: Dogmas Revisited\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Benwood, Claire et al. “Natural Biomaterials and Their Use as Bioinks for Printing Tissues.” Bioengineering (Basel, Switzerland) vol. 8,2 27. 20 Feb. 2021, doi:10.3390\/bioengineering8020027\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7924193\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eNatural Biomaterials and Their Use as Bioinks for Printing Tissues\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Chan, Weng Wan et al. “Additive Biomanufacturing with Collagen Inks.” Bioengineering (Basel, Switzerland) vol. 7,3 66. 1 Jul. 2020, doi:10.3390\/bioengineering7030066\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7552643\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eAdditive Biomanufacturing with Collagen Inks\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Xu, Yujia, and Michele Kirchner. “Collagen Mimetic Peptides.” Bioengineering (Basel, Switzerland) vol. 8,1 5. 5 Jan. 2021, doi:10.3390\/bioengineering8010005\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7824840\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eCollagen Mimetic Peptides\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Xu, Shuaishuai et al. “The role of collagen in cancer: from bench to bedside.” Journal of translational medicine vol. 17,1 309. 14 Sep. 2019, doi:10.1186\/s12967-019-2058-1\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6744664\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eThe role of collagen in cancer: from bench to bedside\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6410095\/\" title=\"Rahman, M Azizur. “Collagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications.” Marine drugs vol. 17,2 118. 14 Feb. 2019, doi:10.3390\/md17020118\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6410095\/\" target=\"_blank\"\u003eCollagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRELATED PRODUCTS\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/attachment-factors-cell-culture\" title=\"Attachment Factors Product Collection\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eAttachment Factors\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Extracellular Matrix Molecules product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" target=\"_blank\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"250 µg - Cat. No. A12678250","offer_id":40783172239512,"sku":"A12678250","price":399.0,"currency_code":"USD","in_stock":true},{"title":"500 µg - Cat. No. A12678500","offer_id":40783172272280,"sku":"A12678500","price":590.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A126781MG","offer_id":40783172305048,"sku":"A126781MG","price":975.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A12678_COL-IVBOVINE.jpg?v=1691006953"},{"product_id":"collagen-v-protein-bovine-placenta","title":"Collagen-V Protein, Bovine Placenta","description":"\u003ch2\u003e\u003cspan data-mce-fragment=\"1\"\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBovine Collagen-V is a natural protein purified from bovine placenta. Collagen-V is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCollagen, a major component of the extracellular matrix, is a fibrous protein that provides tensile strength to tissues giving them structural integrity. Collagens contribute to mechanical properties, organization, and shape of tissues. Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eType V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen contributes to the bone matrix, corneal stroma, and the interstitial matrix of muscles, liver, lungs, and placenta. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eBovine placenta.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eFiltered white lyophilized (freeze-dried) powder. Collagen-V was lyophilized without additives.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003ePurity \u0026gt;\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003cspan\u003e \u003c\/span\u003e98.0%.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eWe recommend to reconstitute the lyophilized Collagen-V in 20 mM acetic acid not less than 1 mg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature.  Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eLyophilized Collagen-V, although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution, Collagen-V should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% \u003ca href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" target=\"_blank\" title=\"HSA \/ BSA PRODUCT COLLECTION\" rel=\"noopener noreferrer\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e.  Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003c\/h2\u003e\n\u003cp\u003e\u003cspan mce-data-marked=\"1\"\u003eType V Collagen, Type 5 Collagen, Collagen-5, COL5A1, Collagen alpha-1(V) chain\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eRESOURCES\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Collagen-V Protein, Bovine Placenta, Product Datasheet (PDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A12677_Collagen-V_Bovine.pdf?v=1629927889\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWant to learn more about collagen? We have compiled the links below that contain information which you may find interesting:\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Collagen type V alpha 1 chain\" href=\"https:\/\/www.uniprot.org\/uniprot\/G3MZI7\" target=\"_blank\"\u003eUniProt Knowledgebase: UniProtKB - G3MZI7 (G3MZI7_BOVIN): Collagen type V alpha 1 chain\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"PubChem Gene Summary: COL5A1 - collagen type V alpha 1 chain (cattle)\" href=\"https:\/\/pubchem.ncbi.nlm.nih.gov\/gene\/100848491\" target=\"_blank\"\u003e\u003cspan data-mce-fragment=\"1\"\u003ePubChem Gene Summary: COL5A1 - collagen type V alpha 1 chain (cattle)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Ricard-Blum, Sylvie. “The collagen family.” Cold Spring Harbor perspectives in biology vol. 3,1 a004978. 1 Jan. 2011, doi:10.1101\/cshperspect.a004978\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3003457\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003e\u003cspan\u003eThe Collagen Family\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Mathew-Steiner, Shomita S et al. “Collagen in Wound Healing.” Bioengineering (Basel, Switzerland) vol. 8,5 63. 11 May. 2021, doi:10.3390\/bioengineering8050063\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8151502\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003e\u003cspan\u003eCollagen In Wound Healing\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Musiime, Moses et al. “Collagen Assembly at the Cell Surface: Dogmas Revisited.” Cells vol. 10,3 662. 16 Mar. 2021, doi:10.3390\/cells10030662\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8002325\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eCollagen Assembly at the Cell Surface: Dogmas Revisited\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Benwood, Claire et al. “Natural Biomaterials and Their Use as Bioinks for Printing Tissues.” Bioengineering (Basel, Switzerland) vol. 8,2 27. 20 Feb. 2021, doi:10.3390\/bioengineering8020027\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7924193\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eNatural Biomaterials and Their Use as Bioinks for Printing Tissues\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Chan, Weng Wan et al. “Additive Biomanufacturing with Collagen Inks.” Bioengineering (Basel, Switzerland) vol. 7,3 66. 1 Jul. 2020, doi:10.3390\/bioengineering7030066\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7552643\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eAdditive Biomanufacturing with Collagen Inks\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Xu, Yujia, and Michele Kirchner. “Collagen Mimetic Peptides.” Bioengineering (Basel, Switzerland) vol. 8,1 5. 5 Jan. 2021, doi:10.3390\/bioengineering8010005\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7824840\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eCollagen Mimetic Peptides\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Xu, Shuaishuai et al. “The role of collagen in cancer: from bench to bedside.” Journal of translational medicine vol. 17,1 309. 14 Sep. 2019, doi:10.1186\/s12967-019-2058-1\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6744664\/\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" target=\"_blank\"\u003eThe role of collagen in cancer: from bench to bedside\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6410095\/\" title=\"Rahman, M Azizur. “Collagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications.” Marine drugs vol. 17,2 118. 14 Feb. 2019, doi:10.3390\/md17020118\" aria-describedby=\"a11y-new-window-external-message\" rel=\"null\" data-mce-href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6410095\/\" target=\"_blank\"\u003eCollagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRELATED PRODUCTS\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/attachment-factors-cell-culture\" title=\"Attachment Factors Product Collection\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eAttachment Factors\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Extracellular Matrix Molecules product collection\" href=\"https:\/\/ilexlife.com\/collections\/extracellular-matrix-molecules\" target=\"_blank\"\u003eExtracellular Matrix Molecules\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"250 µg - Cat. No. A12677250","offer_id":40783258189976,"sku":"A12677250","price":400.0,"currency_code":"USD","in_stock":true},{"title":"500 µg - Cat. No. A12677500","offer_id":40783258222744,"sku":"A12677500","price":590.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A126771MG","offer_id":40783258288280,"sku":"A126771MG","price":975.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A12677_COL-VBOVINE.jpg?v=1691006740"},{"product_id":"mymatrix-msc","title":"myMATRIX MSC: precoated cultureware for mesenchymal stromal cells expansion","description":"\u003cdiv class=\"fusion-title title fusion-title-1 fusion-sep-none fusion-title-text fusion-title-size-two fusion-border-below-title\"\u003e\n\u003cp data-lineheight=\"66.96px\" data-fontsize=\"54\" class=\"fusion-responsive-typography-calculated\"\u003e\u003cspan style=\"color: rgb(255, 0, 0);\"\u003e\u003cstrong\u003eNOTE: This product has been discontinued.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 data-lineheight=\"66.96px\" data-fontsize=\"54\" class=\"fusion-responsive-typography-calculated\"\u003eYOUR CHALLENGE\u003cbr\u003e\n\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cp class=\"fusion-sep-clear\"\u003e\u003cspan\u003eUsing serum-containing media limits the impact of your research on human mesenchymal stromal cells (hMSC) in non-clinical and clinical applications, and impedes the reproducibility of your results. You are interested in reducing or completely eliminating serum content in your media, however cell performance has suffered. You have tried a few coatings or media supplements, but so far there is no clear solution to this issue. Ideally, you want the reliability of more chemically defined media, while maintaining the performance of your hMSC cultures.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"fusion-text fusion-text-6\" style=\"text-align: start;\"\u003e\n\u003ch2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"40\" data-lineheight=\"49.6px\"\u003eTHE SOLUTION\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eSerum-free and chemically defined mesenchymal stromal cell culture with myMATRIX MSC\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg alt=\"myMATRIX MSC precoated T-75 flask\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/myMATRIX-MSC-T75-Flask-easy-to-use_480x480.jpg?v=1631037496\" width=\"480x480\" height=\"480x480\" style=\"float: none;\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"fusion-text fusion-text-7\"\u003e\n\u003cp\u003e\u003cspan\u003emyMATRIX MSC – denovoMATRIX provides a chemically defined cell adhesion-promoting microenvironment offered as pre-coated cultureware, specially formulated for expansion of human mesenchymal stromal cells (hMSC) in serum-free and xeno-free media.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eBENEFITS\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eReady-to-use\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBatch-to-batch consistency\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSerum-free and xeno-free culture\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eCharacteristic phenotype and identity\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eRapid cell proliferation at high viability\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eRobust cell differentiation\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-lineheight=\"49.6px\" data-fontsize=\"40\" class=\"fusion-responsive-typography-calculated\"\u003eRecapitulate key functions of the cellular microenvironment\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003emyMATRIX MSC combines biologically relevant synthetic peptides with sulfated glycosaminoglycan (GAG) analogs to recreate the functional aspects of the cellular microenvironment. A peptide sequence containing ‘RGD’ facilitates cell attachment by stimulating integrin receptors while the GAG analogs support the stabilization of ligand-receptor interactions and storage of growth factors. The coating is manufactured without the use of any animal or human components to enable reliable and reproducible ex vivo cell expansion.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 data-lineheight=\"49.6px\" data-fontsize=\"40\" class=\"\"\u003eAccelerate expansion\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eEfficient in vitro expansion of high quality hMSCs requires optimal growth conditions. myMATRIX MSC can be used in combination with many dissociation reagents, media and supplements and supports cell adhesion, rapid proliferation and high viability in serum-containing as well as serum-free culture conditions. The chemically defined nature of the coating qualifies it for the use in cell manufacturing for clinical research, tissue engineering, cell-based therapies, drug screening and disease modeling.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"Efficient in vitro expansion of high quality MSCs using myMATRIX MSC.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/myMATRIX-MSC_performance_480x480.jpg?v=1631031334\"\u003e\u003c\/div\u003e\n\u003ch2 class=\"fusion-responsive-typography-calculated\" data-lineheight=\"49.6px\" data-fontsize=\"40\"\u003eMaintain high cell quality\u003c\/h2\u003e\n\u003cdiv class=\"fusion-text fusion-text-9\"\u003e\n\u003cp class=\"p2\"\u003e\u003cspan\u003ehMSCs are a heterogeneous population of tissue-specific stem\/progenitor cells. According to the International Society for Cellular Therapy MSCs are defined by three minimal criteria: 1) plastic-adherent growth in serum-containing medium, 2) differentiation capacity into adipocytes, osteocytes and chondrocytes and 3) the absence and presence of specific cell surface antigens. hMSCs cultured on myMATRIX MSC maintain their differentiation and immunomodulatory capacity, characteristic cell morphology and expression of hMSC-specific surface antigens in line with aforementioned standards.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"myMATRIX MSC Product Info Sheet\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/myMATRIX_MSC_Info_Sheet-C03V01.pdf?v=1631035962\" target=\"_blank\"\u003emyMATRIX MSC Product Info Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Application note: myMATRIX MSC – Chemically defined and extracellular matrix-mimetic cultureware for rapid hMSC expansion\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/myMATRIX-MSC-white-paper-C03-V1.pdf?v=1659729863\" target=\"_blank\"\u003eApplication note: myMATRIX MSC – Chemically defined and extracellular matrix-mimetic cultureware for rapid hMSC expansion\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Application note: myMATRIX MSC compatible cells and reagents summary\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/myMATRIX_MSC_-_List_of_compatible_media_cells_and_detachment_solution.pdf?v=1631035617\" target=\"_blank\"\u003eApplication note: myMATRIX MSC compatible cells and reagents summary\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Application note: myMATRIX MSC compatible cells and reagents detailed\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/myMATRIX_MSC_-_List_of_compatible_media_cells_and_detachment_solution_detailed.pdf?v=1631035756\" target=\"_blank\"\u003eApplication note: myMATRIX MSC compatible cells and reagents detailed\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/App-Note_570_growth-factors-FGF2_V01.pdf?v=1659720493\" title=\"Application note: Growth factor stabilization by myMATRIX coatings\" target=\"_blank\"\u003eApplication note: Growth factor stabilization by myMATRIX coatings\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"A Novel Synthetic, Xeno-Free Biomimetic Surface for Serum-Free Expansion of Human Mesenchymal Stromal Cells\" href=\"https:\/\/doi.org\/10.1002\/adbi.202000008\" target=\"_blank\"\u003eDevelopment paper: A Novel Synthetic, Xeno-Free Biomimetic Surface for Serum-Free Expansion of Human Mesenchymal Stromal Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"myMATRIX MSC Material Safety Data Sheet (MSDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MSDS-myMATRIX_MSC-20210413.pdf?v=1631038303\" target=\"_blank\"\u003emyMATRIX MSC Material Safety Data Sheet (MSDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRELATED PRODUCTS\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca title=\"isoMATRIX: precoated cultureware for isolation of MSCs\" href=\"https:\/\/ilexlife.com\/products\/isomatrix\" target=\"_blank\"\u003eisoMATRIX\u003c\/a\u003e: precoated cultureware for isolation of MSCs\u003c\/li\u003e\n\u003cli\u003e\n\u003ca title=\"beadMATRIX: precoated microcarriers for MSCs culture\" href=\"https:\/\/ilexlife.com\/products\/beadmatrix\" target=\"_blank\"\u003ebeadMATRIX\u003c\/a\u003e: precoated microcarriers for MSCs culture\u003c\/li\u003e\n\u003cli\u003e\n\u003ca title=\"Mesenpan: xeno-free, serum-free medium for human mesenchymal stem cells (hMSC) expansion\" href=\"https:\/\/ilexlife.com\/products\/mesenpan-xeno-free-serum-free-medium-for-human-mesenchymal-stem-cells-hmsc-expansion\" target=\"_blank\"\u003eMesenpan\u003c\/a\u003e:\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003exeno-free, serum-free medium for hMSC\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca title=\"PowerStem MSC1: xeno-free, serum-free medium for hMSC\" href=\"https:\/\/ilexlife.com\/products\/powerstem-msc1-serum-free-medium-for-human-mesenchymal-stem-cells-hmsc\" target=\"_blank\"\u003ePowerStem MSC1\u003c\/a\u003e: xeno-free, serum-free medium for hMSC\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/collections\/serum-replacement\" title=\"Serum replacements product collection - substitutes for FBS and other sera\" rel=\"null\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eSerum Replacements\u003c\/a\u003e: easily replace FBS in your cell cultures!\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of denovoMATRIX myMATRIX MSC.\u003c\/em\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"denovoMATRIX","offers":[{"title":"Sample 6-well plate (1 plate) - Cat. No. C0501-SAMPLE","offer_id":44106817274068,"sku":"C0501-SAMPLE","price":50.0,"currency_code":"USD","in_stock":false},{"title":"6-well plates (45-pack) - Cat. No. C0501X9","offer_id":40889504137368,"sku":"C0501X9","price":1642.0,"currency_code":"USD","in_stock":false},{"title":"Sample T-25 flask (1 flask) - Cat. No. C0701-SAMPLE","offer_id":40889504170136,"sku":"C0701-SAMPLE","price":35.0,"currency_code":"USD","in_stock":false},{"title":"T-25 flasks (80-pack) - Cat. No. C0701X16","offer_id":44106837983444,"sku":"C0701X16","price":1642.0,"currency_code":"USD","in_stock":true},{"title":"T-75 flasks (35-pack) - Cat. No. C0601X7","offer_id":40889504202904,"sku":"C0601X7","price":1642.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/myMATRIX-MSC-DenovoMATRIXT-75flask.jpg?v=1631036750"},{"product_id":"mymatrix-ipsc","title":"myMATRIX iPSC - precoated induced pluripotent stem cells cultureware","description":"\u003cdiv class=\"fusion-title title fusion-title-1 fusion-sep-none fusion-title-text fusion-title-size-two fusion-border-below-title\"\u003e\n\u003cp data-lineheight=\"66.96px\" data-fontsize=\"54\" class=\"fusion-responsive-typography-calculated\"\u003e\u003cspan style=\"color: rgb(255, 0, 0);\"\u003e\u003cstrong\u003eNOTE: This product has been discontinued.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 data-lineheight=\"66.96px\" data-fontsize=\"54\" class=\"fusion-responsive-typography-calculated\"\u003eYOUR CHALLENGE\u003cbr\u003e\n\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cp class=\"fusion-sep-clear\"\u003e\u003cspan\u003eUsing animal derived coatings limits the impact of your research on human induced pluripotent stem cells (hiPSC) and impedes the reproducibility of your results. It’s tedious to always freshly coat your plates. You’ve tried a few alternatives which are animal-free, however cell performance suffers, and you end up returning to your previous ‘gold standard’. Ideally, you want the reliability of more defined coatings, while maintaining the performance of your cell culture, without the hassle of pre-coating.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"fusion-text fusion-text-6\" style=\"text-align: start;\"\u003e\n\u003ch2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"40\" data-lineheight=\"49.6px\"\u003eTHE SOLUTION\u003c\/h2\u003e\n\u003cp data-lineheight=\"66.96px\" data-fontsize=\"54\" class=\"fusion-responsive-typography-calculated\"\u003e\u003cstrong\u003eXeno-free and chemically defined pluripotent stem cell culture with myMATRIX iPSC.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"myMATRIX iPSC precoated cultureware plates\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/myMATRIX_iPSC_image_480x480_34aa13f8-6ee3-493c-b462-73eb8fd264d1_480x480.jpg?v=1631031612\" width=\"480x480\" height=\"480x480\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"fusion-text fusion-text-7\"\u003e\n\u003cp\u003e\u003cspan\u003emyMATRIX iPSC – denovoMATRIX provides a chemically defined cell adhesion-promoting microenvironment offered as pre-coated cultureware, specially formulated for expansion of induced pluripotent stem cells (hiPSC).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eBENEFITS\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eReady-to-use\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBatch-to-batch consistency\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAnimal component-free product\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eTypical iPSC morphology and robust proliferation\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eHigh and consistent expression of stemness markers\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePreservation of pluripotency and genetic stability\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-lineheight=\"49.6px\" data-fontsize=\"40\" class=\"fusion-responsive-typography-calculated\"\u003eRECAPITULATE KEY FUNCTIONS OF THE CELLULAR MICROENVIRONMENT\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003emyMATRIX coatings are manufactured without any animal or human components and are ready-to-use. Human induced pluripotent stem cells have a stable karyotype, high expression of stemness genes and are pluripotent after long-term culture on myMATRIX iPSC. The coating combines biologically relevant synthetic peptides with sulfated glycosaminoglycan (GAG) analogs to recreate the functional aspects of the cellular microenvironment.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 data-lineheight=\"49.6px\" data-fontsize=\"40\" class=\"p1 fusion-responsive-typography-calculated\"\u003e\u003cspan class=\"s1\"\u003eMaintaining pluripotency and genetic stability\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eConsistent and high expression of stemness markers\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"Consistent and high expression of stemness markers\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/iPSC_stemness_markers_480x480.png?v=1631044162\"\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSurface-antigen expression patterns of iPSCs cultured on myMATRIX iPSC show high expression of pluripotency markers as analyzed by Fluorescence-activated cell sorting (FACS).\u003c\/li\u003e\n\u003cli\u003eiPSCs on myMATRIX show high expression of a series of iPSC-specific genes such as OCT4, Nanog, Sox2, FGF2 and Lin28 throughout the culture estimated by quantitative RT-PCR (see white paper).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"text-align: start;\"\u003e\u003cstrong\u003ePreservation of genetic stability\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg style=\"float: none;\" height=\"480x480\" width=\"480x480\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/iPSCs_Preservation_of_genetic_stability_480x480.jpg?v=1631044225\" alt=\"Preservation of genetic stability\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eiPSCs cultured on myMATRIX iPSC are genetically stable.\u003c\/li\u003e\n\u003cli\u003eNormal, healthy karyotypes after long-term culture of 20 passages.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"text-align: start;\"\u003e\u003cstrong\u003ePreservation of differentiation capacity\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cstrong\u003e\u003cimg style=\"float: none;\" height=\"480x480\" width=\"480x480\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/myMATRIX-iPSC_differentiation-panel_2021-1200x434_480x480.png?v=1631044277\" alt=\"Preservation of differentiation capacity\"\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\n\u003cul\u003e\n\u003cli\u003eiPSCs on myMATRIX maintain their differentiation potential and therefore, form all three germ layers after long-term culture.\u003c\/li\u003e\n\u003cli\u003eCells were differentiated after 20 passages in culture.\u003c\/li\u003e\n\u003cli\u003eDifferentiated cells (DAPI, blue) and germ layer-specific marker expression were visualized by immunostaining: Mesoderm – Brachyury (green), CXCR4 (red) Endoderm – GATA6 (green), Sox17 (red) Endoderm – Nestin (green), Pax6 (red).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/myMATRIX_iPSC_Info_Sheet-C05V2.pdf?v=1631044406\" title=\"myMATRIX iPSC Product Info Sheet\" target=\"_blank\"\u003emyMATRIX iPSC Product Info Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/myMATRIX_iPSC-User_Guide-C05_V1.pdf?v=1631044523\" title=\"myMATRIX iPSC User Guide and Protocols\" target=\"_blank\"\u003emyMATRIX iPSC User Guide and Protocols\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/myMATRIX-iPSC-white-paper-C05-V02.pdf?v=1631044662\" title=\"White Paper: myMATRIX iPSC – Xeno-free and chemically defined surface for high-quality pluripotent stem cell culture\" target=\"_blank\"\u003eWhite Paper: myMATRIX iPSC – Xeno-free and chemically defined surface for high-quality pluripotent stem cell culture\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/MSDS-myMATRIX_iPSC-20210903_V003.pdf?v=1631044765\" title=\"myMATRIX iPSC Material Safety Data Sheet (MSDS)\" target=\"_blank\"\u003emyMATRIX iPSC Material Safety Data Sheet (MSDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"beadMATRIX+: precoated microcarriers for iPSCs culture\" href=\"https:\/\/ilexlife.com\/products\/beadmatrix-precoated-microcarriers-for-ipscs-culture\" target=\"_blank\"\u003ebeadMATRIX+: precoated microcarriers for iPSCs culture\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Serum-free cell culture media\" href=\"https:\/\/ilexlife.com\/collections\/serum-free-cell-culture-media\" target=\"_blank\"\u003eSerum-free cell culture media\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca title=\"Serum replacements product collection - substitutes for FBS and other sera\" href=\"https:\/\/ilexlife.com\/collections\/serum-replacement\" target=\"_blank\"\u003eSerum Replacements\u003c\/a\u003e: easily replace FBS in your cell cultures!\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of denovoMATRIX myMATRIX iPSC.\u003c\/em\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"denovoMATRIX","offers":[{"title":"Sample 6-well plate (1 plate) - Cat. No. C0505-SAMPLE","offer_id":44106846273748,"sku":"C0505-SAMPLE","price":25.0,"currency_code":"USD","in_stock":false},{"title":"6-well plates (140-pack) - Cat. No. C0505X28","offer_id":43136088834260,"sku":"C0505X28","price":1720.0,"currency_code":"USD","in_stock":false},{"title":"96-well plates (125-pack) - Cat. No. C0105X25","offer_id":40890073317528,"sku":"C0105X25","price":1642.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/myMATRIXiPSCimage.jpg?v=1631044936"},{"product_id":"mouse-m-csf-protein","title":"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, E. coli Recombinant Protein","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eMacrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, E. coli Recombinant Protein\u003c\/em\u003e is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and has a total molecular mass of 36.4 kDa. \u003c\/span\u003e\u003cspan\u003eM-CSF is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMacrophage colony-stimulating factor (M-CSF) is a\u003c\/span\u003e\u003cspan\u003e c\u003c\/span\u003e\u003cspan\u003eytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P07141\" title=\"UniProt Knowledgebase: UniProtKB - P07141 (CSF1_MOUSE)\" target=\"_blank\"\u003eUniProtKB - P07141 (CSF1_MOUSE)\u003c\/a\u003e].\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e81627-83-0\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBioactive Murine M-CSF, \u003c\/span\u003e\u003cspan\u003eCSF-1, CSF1 gene product, MCSF\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eAmino Acid Sequence\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eExpressed in \u003cem\u003eE. coli.\u003c\/em\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a sterile (0.2 µm) filtered solution containing 10mM sodium phosphate, 50mM sodium chloride, pH 7.5.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eGreater than 95.0% as determined by SDS-PAGE.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eBiological Activity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe ED\u003c\/span\u003e\u003csub\u003e50\u003c\/sub\u003e\u003cspan\u003e, as calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells is 2.1 ng\/ml, corresponding to a specific activity of 4.8 x 10\u003c\/span\u003e\u003csup\u003e5\u003c\/sup\u003e\u003cspan\u003e units\/mg \u003cem\u003e(NOTE: May vary with each lot number. Please refer to the datasheet enclosed with your product)\u003c\/em\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eIt is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100 µg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature. Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eLyophilized M-CSF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, M-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. \u003c\/span\u003e\u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"Product Datasheet (PDS): Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, E. coli Recombinant Protein\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A3439_M-CSF_Mouse_E._coli_Recombinant.docx.pdf?v=1747339225\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A3439_M-CSF_Mouse_E._coli.pdf?v=1631294903\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, E. coli Recombinant Protein Product Datasheet (PDS)\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/collections\/cytokines\" title=\"Cytokines \u0026amp; Growth Factors Product Collection\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003eCytokines \u0026amp; Growth Factors\u003c\/span\u003e\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"KGF (FGF-7) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/kgf-fgf-7-human-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003c\/a\u003e\u003cem\u003e\u003c\/em\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/products\/isokine-m-csf-human-animal-free-recombinant-protein\" title=\"ISOkine® M-CSF Human, Animal-Free Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003eISOkine® M-CSF Human, Animal-Free Recombinant Protein\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, E. coli Recombinant Protein\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"Interleukin-4 (IL-4) Human, P. pastoris Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/interleukin-4-il-4-human-p-pastoris-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/human-m-csf-his-tag-e-coli-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, E. coli Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, His Tag, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, Baculovirus Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-baculovirus-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, Baculovirus Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/human-m-csf-sf9-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, Sf9 Insect Cells Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-sf9-baculovirus-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Rat, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-hek293-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, HEK293 Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Rat, HEK293 Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"2 µg - Cat. No. A3439002","offer_id":40913021501592,"sku":"A3439002","price":95.0,"currency_code":"USD","in_stock":true},{"title":"10 µg - Cat. No. A3439010","offer_id":40913021534360,"sku":"A3439010","price":195.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A34391MG","offer_id":40913333813400,"sku":"A34391MG","price":3825.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/products\/A3439SM.jpg?v=1675887179"},{"product_id":"human-m-csf-e-coli-recombinant-protein","title":"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, E. coli Recombinant Protein","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eMacrophage Colony-Stimulating Factor 1 (M-CSF) Human, E. coli Recombinant Protein\u003c\/em\u003e is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and has a total molecular mass of 37.1 kDa. Human M-CSF is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMacrophage colony-stimulating factor (M-CSF) is a\u003c\/span\u003e\u003cspan\u003e c\u003c\/span\u003e\u003cspan\u003eytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: \u003ca title=\"UniProt Knowledgebase: UniProtKB - A0A024R0A1 (A0A024R0A1_HUMAN): Macrophage colony-stimulating factor 1 (human)\" href=\"https:\/\/www.uniprot.org\/uniprot\/A0A024R0A1\" target=\"_blank\"\u003eUniProtKB - A0A024R0A1 (CSF1_HUMAN)\u003c\/a\u003e].\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e81627-83-0\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBioactive Human M-CSF, \u003c\/span\u003e\u003cspan\u003eCSF-1, CSF1 gene product, MCSF\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eAmino Acid Sequence\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eE. coli\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized with 10mM sodium Phosphate, pH-8.0 and 50mM NaCl.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eGreater than 95.0% as determined by SDS-PAGE.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eBiological Activity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe ED\u003c\/span\u003e\u003csub\u003e50\u003c\/sub\u003e\u003cspan\u003e, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells, was found to be 1.15 ng\/ml corresponding to a specific activity of 8.7x10\u003csup\u003e5\u003c\/sup\u003e Units\/mg \u003cem\u003e(Specific activity may vary slightly between each lot, please refer to the datasheet enclosed with your shipment).\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eIt is recommended to reconstitute the lyophilized M-CSF in sterile 18M-cm H2O not less than 100 µg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature. Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eLyophilized M-CSF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C. \u003c\/span\u003e\u003cstrong\u003ePlease prevent freeze-thaw cycles. \u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A3308_M-CSF_Human_E._coli_975b201f-d1d7-4c48-9a28-a6892959c04c.pdf?v=1687196890\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, E. coli Recombinant Protein Product Datasheet (PDS)\" target=\"_blank\"\u003e Product Datasheet (PDS)\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/collections\/cytokines\" title=\"Cytokines \u0026amp; Growth Factors Product Collection\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003eCytokines \u0026amp; Growth Factors\u003c\/span\u003e\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"KGF (FGF-7) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/kgf-fgf-7-human-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003c\/a\u003e\u003cem\u003e\u003c\/em\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/products\/isokine-m-csf-human-animal-free-recombinant-protein\" title=\"ISOkine® M-CSF Human, Animal-Free Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003eISOkine® M-CSF Human, Animal-Free Recombinant Protein\u003c\/span\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/human-m-csf-his-tag-e-coli-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, E. coli Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, His Tag, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, Baculovirus Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-baculovirus-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, Baculovirus Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/human-m-csf-sf9-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, Sf9 Insect Cells Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Mouse, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-sf9-baculovirus-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Rat, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-hek293-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, HEK293 Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Rat, HEK293 Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"10 µg - Cat. No. A3308010","offer_id":41073931124888,"sku":"A3308010","price":195.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A33081MG","offer_id":41073931157656,"sku":"A33081MG","price":3750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A3308_M-CSFHUMANE.COLI.jpg?v=1691182765"},{"product_id":"rat-m-csf-e-coli-recombinant-protein","title":"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, E. coli Recombinant Protein","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eMacrophage Colony-Stimulating Factor 1 (M-CSF) Rat, E. coli Recombinant Protein\u003c\/em\u003e is a non-glycosylated homodimer, containing 2 x 155 amino acids and has a total molecular mass of 36.2 kDa. M-CSF is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMacrophage colony-stimulating factor (M-CSF) is a\u003c\/span\u003e\u003cspan\u003e c\u003c\/span\u003e\u003cspan\u003eytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: \u003ca title=\"UniProt Knowledgebase: UniProtKB - Q8JZQ0 (CSF1_RAT): Macrophage colony-stimulating factor 1 (rat)\" href=\"https:\/\/www.uniprot.org\/uniprot\/Q8JZQ0\" target=\"_blank\"\u003eUniProtKB - Q8JZQ0 (CSF1_RAT)\u003c\/a\u003e].\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e81627-83-0\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBioactive Rat M-CSF, \u003c\/span\u003e\u003cspan\u003eCSF-1, CSF1 gene product, MCSF\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eAmino Acid Sequence\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF  FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eE. coli\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSterile filtered white lyophilized (freeze-dried) powder. Lyophilized from a sterile filtered aqueous solution containing 10mM Na\u003c\/span\u003e\u003csub\u003e3\u003c\/sub\u003e\u003cspan\u003ePO\u003c\/span\u003e\u003csub\u003e4\u003c\/sub\u003e\u003cspan\u003e, pH 7.5.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eGreater than 95.0% as determined by SDS-PAGE.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eBiological Activity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe activity as determined by dose-dependent induction of M-NFS-60 cell proliferation is 1.65 ng\/ml. This corresponds to an expected specific activity of 6.1x10\u003c\/span\u003e\u003csup\u003e5\u003c\/sup\u003e\u003cspan\u003e units\/mg.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eIt is recommended to reconstitute the lyophilized M-CSF in sterile 18M-cm H2O not less than 100 µg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature. Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eLyophilized M-CSF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, M-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% \u003ca href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" title=\"HSA \/ BSA product collection\" target=\"_blank\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, E. coli Recombinant Protein Product Datasheet (PDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A3856_M-CSF_Rat_E._coli.pdf?v=1633365188\" target=\"_blank\"\u003e Product Datasheet (PDS)\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"noopener\" title=\"Cytokines \u0026amp; Growth Factors Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/cytokines\" target=\"_blank\"\u003e\u003cspan\u003eCytokines \u0026amp; Growth Factors\u003c\/span\u003e\u003c\/a\u003e\u003ca aria-describedby=\"a11y-new-window-message\" href=\"https:\/\/ilexlife.com\/products\/kgf-fgf-7-human-e-coli-recombinant-protein\" title=\"KGF (FGF-7) Human, E. coli Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003e\u003c\/a\u003e\u003cem\u003e\u003c\/em\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"noopener\" title=\"ISOkine® M-CSF Human, Animal-Free Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/isokine-m-csf-human-animal-free-recombinant-protein\" target=\"_blank\"\u003e\u003cspan\u003eISOkine® M-CSF Human, Animal-Free Recombinant Protein\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca aria-describedby=\"a11y-new-window-message\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-e-coli-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, E. coli Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eM-CSF Human, E. coli Recombinant Protein\u003c\/a\u003e\u003ca aria-describedby=\"a11y-new-window-message\" href=\"https:\/\/ilexlife.com\/products\/interleukin-4-il-4-human-p-pastoris-recombinant-protein\" title=\"Interleukin-4 (IL-4) Human, P. pastoris Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-his-tag-e-coli-recombinant-protein\" target=\"_blank\"\u003eM-CSF Human, His Tag, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-baculovirus-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, Baculovirus Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eM-CSF Human, Baculovirus Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, Sf9 Insect Cells Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-sf9-recombinant-protein\" target=\"_blank\"\u003eM-CSF Human, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, E. coli Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eM-CSF Mouse, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-sf9-baculovirus-recombinant-protein\" target=\"_blank\"\u003eM-CSF Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, HEK293 Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-hek293-recombinant-protein\" target=\"_blank\"\u003eM-CSF Rat, HEK293 Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"10 µg - Cat. No. A3856010","offer_id":41074207752344,"sku":"A3856010","price":195.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A38561MG","offer_id":41074207785112,"sku":"A38561MG","price":4395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A3856_M-CSF.jpg?v=1691513713"},{"product_id":"rat-m-csf-hek293-recombinant-protein","title":"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, HEK293 Recombinant Protein","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eMacrophage Colony-Stimulating Factor 1 (M-CSF) Rat, HEK293 Recombinant Protein\u003c\/em\u003e is a secreted protein (amino acids Glu33-Arg254). M-CSF is a disulfide-linked homodimer containing 2 x 222 amino acid chains.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMacrophage colony-stimulating factor (M-CSF) is a\u003c\/span\u003e\u003cspan\u003e c\u003c\/span\u003e\u003cspan\u003eytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: \u003ca title=\"UniProt Knowledgebase: UniProtKB - Q8JZQ0 (CSF1_RAT): Macrophage colony-stimulating factor 1 (rat)\" href=\"https:\/\/www.uniprot.org\/uniprot\/Q8JZQ0\" target=\"_blank\"\u003eUniProtKB - Q8JZQ0 (CSF1_RAT)\u003c\/a\u003e].\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e81627-83-0\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBioactive Rat M-CSF, \u003c\/span\u003e\u003cspan\u003eCSF-1, CSF1 gene product, MCSF\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eHEK293 cells\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSterile filtered white lyophilized (freeze-dried) powder. The recombinant M-CSF was lyophilized after extensive dialysis against PBS.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eGreater than 95.0% as determined by SDS-PAGE.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eBiological Activity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe ED\u003csub\u003e50 \u003c\/sub\u003eof 3-4 ng\/ml is measured by its ability to stimulate the proliferation of mouse M-NFS-60 cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eIt is recommended to reconstitute the M-CSF in sterile PBS not less than 100 µg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature.  Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eLyophilized M-CSF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, M-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% \u003ca href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" title=\"BSA\/HSA Product Collection\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A3046_M-CSF_Rat_HEK293.pdf?v=1633368032\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, HEK293 Recombinant Protein Product Datasheet (PDS)\" target=\"_blank\"\u003e Product Datasheet (PDS)\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"noopener\" title=\"Cytokines \u0026amp; Growth Factors Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/cytokines\" target=\"_blank\"\u003e\u003cspan\u003eCytokines \u0026amp; Growth Factors\u003c\/span\u003e\u003c\/a\u003e\u003ca aria-describedby=\"a11y-new-window-message\" href=\"https:\/\/ilexlife.com\/products\/kgf-fgf-7-human-e-coli-recombinant-protein\" title=\"KGF (FGF-7) Human, E. coli Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003e\u003c\/a\u003e\u003cem\u003e\u003c\/em\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"noopener\" title=\"ISOkine® M-CSF Human, Animal-Free Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/isokine-m-csf-human-animal-free-recombinant-protein\" target=\"_blank\"\u003e\u003cspan\u003eISOkine® M-CSF Human, Animal-Free Recombinant Protein\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca aria-describedby=\"a11y-new-window-message\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-e-coli-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, E. coli Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eM-CSF Human, E. coli Recombinant Protein\u003c\/a\u003e\u003ca aria-describedby=\"a11y-new-window-message\" href=\"https:\/\/ilexlife.com\/products\/interleukin-4-il-4-human-p-pastoris-recombinant-protein\" title=\"Interleukin-4 (IL-4) Human, P. pastoris Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-his-tag-e-coli-recombinant-protein\" target=\"_blank\"\u003eM-CSF Human, His Tag, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-baculovirus-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, Baculovirus Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eM-CSF Human, Baculovirus Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, Sf9 Insect Cells Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-sf9-recombinant-protein\" target=\"_blank\"\u003eM-CSF Human, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, E. coli Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eM-CSF Mouse, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-sf9-baculovirus-recombinant-protein\" target=\"_blank\"\u003eM-CSF Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca aria-describedby=\"a11y-new-window-message\" href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-e-coli-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, E. coli Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eM-CSF Rat, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"2 µg - Cat. No. A3046002","offer_id":41074497519768,"sku":"A3046002","price":130.0,"currency_code":"USD","in_stock":true},{"title":"10 µg - Cat. No. A3046010","offer_id":41074497552536,"sku":"A3046010","price":195.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A30461MG","offer_id":41074497585304,"sku":"A30461MG","price":4575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A3046_M-CSFRATHEK.jpg?v=1691008021"},{"product_id":"human-m-csf-his-tag-e-coli-recombinant-protein","title":"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, E. coli Recombinant Protein","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eMacrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, E. coli Recombinant Protein\u003c\/em\u003e is a single, non-glycosylated, polypeptide chain containing 179 amino acids (33-190 a.a.) and has a total molecular mass of 20.7 kDa. The M-CSF is fused to a 20 amino acid His Tag at N-terminus and is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMacrophage colony-stimulating factor (M-CSF) is a\u003c\/span\u003e\u003cspan\u003e c\u003c\/span\u003e\u003cspan\u003eytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: \u003ca title=\"UniProt Knowledgebase: UniProtKB - A0A024R0A1 (A0A024R0A1_HUMAN): Macrophage colony-stimulating factor 1 (human)\" href=\"https:\/\/www.uniprot.org\/uniprot\/A0A024R0A1\" target=\"_blank\"\u003eUniProtKB - A0A024R0A1 (CSF1_HUMAN)\u003c\/a\u003e].\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e81627-83-0\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eHis-Tagged M-CSF, Human M-CSF, \u003c\/span\u003e\u003cspan\u003eCSF-1, CSF1 gene product, MCSF\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eAmino Acid Sequence\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMGSSHHHHHH SSGLVPRGSH MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ DVVTKPDCN.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003eE. coli\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSterile filtered colorless solution. The M-CSF protein solution contains 20mM Tris-HCl, pH-8, 2mM DTT, and 10% Glycerol.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eGreater than 90.0% as determined by SDS-PAGE.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eShipped on regular ice packs. Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eStore at 4°C if entire vial will be used within 2-4 weeks. For longer periods of time, store frozen at -20°C. For long term storage it is recommended to add a carrier protein (0.1% \u003ca title=\"HSA \/ BSA product collection\" href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" target=\"_blank\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003eAvoid multiple freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A3695_M-CSF_Human_His_Tag_E._coli.pdf?v=1633380913\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, E. coli Recombinant Protein Product Datasheet (PDS)\" target=\"_blank\"\u003e Product Datasheet (PDS)\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/collections\/cytokines\" title=\"Cytokines \u0026amp; Growth Factors Product Collection\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003eCytokines \u0026amp; Growth Factors\u003c\/span\u003e\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"KGF (FGF-7) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/kgf-fgf-7-human-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003c\/a\u003e\u003cem\u003e\u003c\/em\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/products\/isokine-m-csf-human-animal-free-recombinant-protein\" title=\"ISOkine® M-CSF Human, Animal-Free Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003eISOkine® M-CSF Human, Animal-Free Recombinant Protein\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, Baculovirus Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-baculovirus-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, Baculovirus Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/human-m-csf-sf9-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, Sf9 Insect Cells Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Mouse, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-sf9-baculovirus-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Rat, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-hek293-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, HEK293 Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Rat, HEK293 Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"25 µg - Cat. No. A3695025","offer_id":41075422625944,"sku":"A3695025","price":215.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A36951MG","offer_id":41075422691480,"sku":"A36951MG","price":2195.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A3695_M-CSF.jpg?v=1691430013"},{"product_id":"human-m-csf-baculovirus-recombinant-protein","title":"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, Baculovirus Recombinant Protein","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eMacrophage Colony-Stimulating Factor 1 (M-CSF) Human, Baculovirus Recombinant Protein\u003c\/em\u003e is a disulfide linked homodimer, glycosylated, polypeptide chain containing 2 x 149 amino acids and has a total molecular mass of 42 kDa. The M-CSF is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMacrophage colony-stimulating factor (M-CSF) is a\u003c\/span\u003e\u003cspan\u003e c\u003c\/span\u003e\u003cspan\u003eytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/A0A024R0A1\" title=\"UniProt Knowledgebase: UniProtKB - A0A024R0A1 (A0A024R0A1_HUMAN): Macrophage colony-stimulating factor 1 (human)\" target=\"_blank\"\u003eUniProtKB - A0A024R0A1 (CSF1_HUMAN)\u003c\/a\u003e].\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e81627-83-0\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBioactive Human M-CSF, \u003c\/span\u003e\u003cspan\u003eCSF-1, CSF1 gene product, MCSF\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eAmino Acid Sequence\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eEEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQ.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp class=\"product-specs-content\"\u003eBaculovirus infected Silkworm.\u003c\/p\u003e\n\u003cdiv class=\"product-specs-content\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSterile filtered white lyophilized (freeze-dried) powder. The lyophilized protein (1 mg\/ml) was lyophilized with 20mM phosphate buffer, 1% HSA and 3% mannitol.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eGreater than 95.0% as determined by analysis by RP-HPLC and\u003c\/span\u003e\u003cspan\u003e SDS-PAGE.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eBiological Activity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe ED\u003csub\u003e50\u003c\/sub\u003e, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found \u0026lt; 3 ng\/ml, corresponding to a specific activity of less than 333,333.33 units\/mg.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eIt is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100 µg\/ml, which can then be further diluted to other aqueous solutions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eAt ambient temperature. Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eLyophilized M-CSF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, M-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. \u003c\/span\u003e\u003cstrong\u003ePlease prevent freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, Baculovirus Recombinant Protein Product Datasheet (PDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A3637_M-CSF_Human_Baculovirus.pdf?v=1633385181\" target=\"_blank\"\u003e Product Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/collections\/cytokines\" title=\"Cytokines \u0026amp; Growth Factors Product Collection\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003eCytokines \u0026amp; Growth Factors\u003c\/span\u003e\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"KGF (FGF-7) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/kgf-fgf-7-human-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003c\/a\u003e\u003cem\u003e\u003c\/em\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/products\/isokine-m-csf-human-animal-free-recombinant-protein\" title=\"ISOkine® M-CSF Human, Animal-Free Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003eISOkine® M-CSF Human, Animal-Free Recombinant Protein\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, E. coli Recombinant Protein\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"Interleukin-4 (IL-4) Human, P. pastoris Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/interleukin-4-il-4-human-p-pastoris-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/human-m-csf-his-tag-e-coli-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, E. coli Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, His Tag, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/human-m-csf-sf9-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, Sf9 Insect Cells Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Mouse, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-sf9-baculovirus-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Rat, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-hek293-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, HEK293 Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Rat, HEK293 Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"10 µg - Cat. No. A3637010","offer_id":41075760464024,"sku":"A3637010","price":195.0,"currency_code":"USD","in_stock":true},{"title":"1 mg - Cat. No. A36371MG","offer_id":41075760496792,"sku":"A36371MG","price":4595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A3637_M-CSFHUMAN_BAC.jpg?v=1691182718"},{"product_id":"mouse-m-csf-sf9-baculovirus-recombinant-protein","title":"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, His Tag, Sf9 Insect Cells Recombinant Protein","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eMacrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, Sf9 Insect Cells Recombinant Protein\u003c\/em\u003e is a single, glycosylated polypeptide chain containing 164 amino acids (33-187 aa) and has a molecular mass of 19.1 kDa. The M-CSF is fused to a 6 amino acid His tag at C-terminus and is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMacrophage colony-stimulating factor (M-CSF) is a\u003c\/span\u003e\u003cspan\u003e c\u003c\/span\u003e\u003cspan\u003eytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/P07141\" title=\"UniProt Knowledgebase: UniProtKB - P07141 (CSF1_MOUSE)\" target=\"_blank\"\u003eUniProtKB - P07141 (CSF1_MOUSE)\u003c\/a\u003e].\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e81627-83-0\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBioactive Murine M-CSF, \u003c\/span\u003e\u003cspan\u003eCSF-1, CSF1 gene product, MCSF\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eAmino Acid Sequence\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eADPKEVSEHC SHMIGNGHLK VLQQLIDSQM ETSCQIAFEF VDQEQLDDPV CYLKKAFFLV\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eQDIIDETMRF KDNTPNANAT ERLQELSNNL NSCFTKDYEE QNKACVRTFH ETPLQLLEKI\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eKNFFNETKNL LEKDWNIFTK NCNNSFAKCS SRDVVTKP\u003c\/span\u003e\u003cspan\u003eHH HHHH.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBaculovirus infected Sf9 (Spodoptera frugiperda) insect cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSterile Filtered colorless solution. The M-CSF solution (1 mg\/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eGreater than 90.0% as determined by SDS-PAGE.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eBiological Activity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eDetermined by cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. ED\u003csub\u003e50\u003c\/sub\u003e range for this effect is ≤ 4 ng\/ml.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eShipped on regular ice packs. Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003eStore at 4°C if entire vial will be used within 2-4 weeks. For longer periods of time, store frozen at -20°C. For long term storage it is recommended to add a carrier protein (0.1% \u003ca href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" title=\"HSA \/ BSA Product Collection\" target=\"_blank\"\u003eHSA or BSA\u003c\/a\u003e). \u003cstrong\u003eAvoid multiple freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, His Tag, Sf9 Insect Cells Recombinant Protein Product Datasheet (PDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A31141_M-CSF_Mouse_Sf9_Baculovirus.pdf?v=1633622978\" target=\"_blank\"\u003e Product Datasheet (PDS)\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/collections\/cytokines\" title=\"Cytokines \u0026amp; Growth Factors Product Collection\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003eCytokines \u0026amp; Growth Factors\u003c\/span\u003e\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"KGF (FGF-7) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/kgf-fgf-7-human-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003c\/a\u003e\u003cem\u003e\u003c\/em\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/products\/isokine-m-csf-human-animal-free-recombinant-protein\" title=\"ISOkine® M-CSF Human, Animal-Free Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003eISOkine® M-CSF Human, Animal-Free Recombinant Protein\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, E. coli Recombinant Protein\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"Interleukin-4 (IL-4) Human, P. pastoris Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/interleukin-4-il-4-human-p-pastoris-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/human-m-csf-his-tag-e-coli-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, E. coli Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, His Tag, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, Baculovirus Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-baculovirus-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, Baculovirus Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/human-m-csf-sf9-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, Sf9 Insect Cells Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Mouse, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Rat, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-hek293-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, HEK293 Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Rat, HEK293 Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"10 µg - A31141010","offer_id":41075991347352,"sku":"A31141010","price":215.0,"currency_code":"USD","in_stock":true},{"title":"100 µg - A31141100","offer_id":41075991380120,"sku":"A31141100","price":1215.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A31141_M-CSF.jpg?v=1691429908"},{"product_id":"human-m-csf-sf9-recombinant-protein","title":"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, Sf9 Insect Cells Recombinant Protein","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eMacrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/em\u003e is a single, glycosylated polypeptide chain containing 231 amino acids (33-255 a.a.) and has a molecular mass of 26.1 kDa (molecular size on SDS-PAGE will appear at approximately 28-40 kDa). The M-CSF is expressed with an 8 amino acid His tag at C-Terminus and is purified by proprietary chromatographic techniques.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSummary\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMacrophage colony-stimulating factor (M-CSF) is a\u003c\/span\u003e\u003cspan\u003e c\u003c\/span\u003e\u003cspan\u003eytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [Reference: \u003ca rel=\"null\" aria-describedby=\"a11y-new-window-external-message\" href=\"https:\/\/www.uniprot.org\/uniprot\/A0A024R0A1\" title=\"UniProt Knowledgebase: UniProtKB - A0A024R0A1 (A0A024R0A1_HUMAN): Macrophage colony-stimulating factor 1 (human)\" target=\"_blank\"\u003eUniProtKB - A0A024R0A1 (CSF1_HUMAN)\u003c\/a\u003e].\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eCAS Registry No.\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e81627-83-0\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eOther Names\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBioactive Human M-CSF, \u003c\/span\u003e\u003cspan\u003eCSF-1, CSF1 gene product, MCSF\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eAmino Acid Sequence\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eEEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQD VVTKPDCNCL YPKAIPSSDP ASVSPHQPLA PSMAPVAGLT WEDSEGTEGS SLLPGEQPLH TVDPGSAKQR PPR\u003c\/span\u003e\u003cspan\u003eLEHHHHH H\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSource\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSf9 (Spodoptera frugiperda) insect cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSterile Filtered colorless solution. The M-CSF solution \u003c\/span\u003eprotein solution (0.25 mg\/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.\u003c\/p\u003e\n\u003ch2\u003ePurity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eGreater than 95.0% as determined by SDS-PAGE.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eBiological Activity\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eBioactivity is determined in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cell. The ED\u003csub\u003e50\u003c\/sub\u003e for this effect is less or equal to 3 ng\/ml.\u003c\/p\u003e\n\u003ch2\u003eShipping\u003c\/h2\u003e\n\u003cp\u003eShipped on regular ice packs. Upon receipt, store the product at the temperature recommended below.\u003c\/p\u003e\n\u003ch2\u003eStorage\/Expiration\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at 4°C if entire vial will be used within 2-4 weeks. For longer periods of time, s\u003c\/span\u003e\u003cspan\u003etore frozen at -20°C. \u003c\/span\u003e\u003cspan\u003eFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). \u003c\/span\u003e\u003cstrong\u003eAvoid multiple freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A31015_M-CSF_Human_His_Tag_Sf9_Insect_Cells.pdf?v=1633623999\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, Sf9 Insect Cells Recombinant Protein Product Datasheet (PDS)\" target=\"_blank\"\u003e Product Datasheet (PDS)\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/collections\/cytokines\" title=\"Cytokines \u0026amp; Growth Factors Product Collection\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003eCytokines \u0026amp; Growth Factors\u003c\/span\u003e\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"KGF (FGF-7) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/kgf-fgf-7-human-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003c\/a\u003e\u003cem\u003e\u003c\/em\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/isokine-m-csf-human-animal-free-recombinant-protein\" target=\"_blank\" title=\"ISOkine® M-CSF Human, Animal-Free Recombinant Protein\" rel=\"noopener\"\u003e\u003cspan\u003eISOkine® M-CSF Human, Animal-Free Recombinant Protein\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, E. coli Recombinant Protein\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"Interleukin-4 (IL-4) Human, P. pastoris Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/interleukin-4-il-4-human-p-pastoris-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/human-m-csf-his-tag-e-coli-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, His Tag, E. coli Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, His Tag, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Human, Baculovirus Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/human-m-csf-baculovirus-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Human, Baculovirus Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Mouse, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/mouse-m-csf-sf9-baculovirus-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Mouse, His Tag, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-e-coli-recombinant-protein\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Rat, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/rat-m-csf-hek293-recombinant-protein\" title=\"Macrophage Colony-Stimulating Factor 1 (M-CSF) Rat, HEK293 Recombinant Protein\" rel=\"noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003eM-CSF Rat, HEK293 Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ilex Life Sciences","offers":[{"title":"10 µg - Cat. No. A31015010","offer_id":41092318920856,"sku":"A31015010","price":215.0,"currency_code":"USD","in_stock":true},{"title":"100 µg - Cat. No. A31015100","offer_id":41092318953624,"sku":"A31015100","price":1175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/A31015_M-CSF.jpg?v=1691429880"},{"product_id":"3-d-life-togro-hydrogel-kit","title":"3-D Life ToGro Hydrogel Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e \u003cem\u003eToGro\u003c\/em\u003e Hydrogel Kit\u003c\/strong\u003e contains reagents for the preparation of \u003ca href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" title=\"3-D Life Biomimetic Hydrogels\" rel=\"noopener\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e hydrogels\u003c\/a\u003e with a soft stiffness (400 Pa; shear modulus) and a RGD Peptide concentration of 0.4 mmol\/L. The gel composition allows three-dimensional spreading and migration of most cells.\u003c\/p\u003e\n\u003cp\u003eThe gel is formed by the crosslinking of modified dextran carrying the cell adhesion motif RGD (RGD-Dextran) with CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on RGD-Dextran resulting in gel formation within 20 min. The formation of hydrogel is performed at physiological pH for optimal cell compatibility. CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide. The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9. It allows cells to spread and migrate within the gel if they express the indicated MMPs.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e3-D Life ToGro\u003c\/em\u003e hydrogels can be dissolved by the addition of dextranase (\u003ca title=\"3-D Life Dextranase Product Page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-dextranase\" target=\"_blank\"\u003e3-D Life Dextranase Cat. No. D10-1\u003c\/a\u003e), which allows recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or further cultivation. For preparation of gels follow the instructions of the \u003cem\u003e3-D Life ToGro\u003c\/em\u003e Protocol GP-3 “Preparation of \u003cem\u003e3-D Life ToGro\u003c\/em\u003e Hydrogel”. The document can be downloaded below.\u003c\/p\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cp\u003e3D cell culture: 3-dimensional cultivation of various cell types, spheroids, tissue samples and similar requiring a cell-adhesive and cell-degradable matrix for cell growth and differentiation.\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003eAllows formation of 2.4 ml \u003cem\u003e3-D Life ToGro\u003c\/em\u003e Hydrogel.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable style=\"width: 99.9987%; height: 117.5px;\" id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003cth style=\"width: 31.161%; height: 19.5833px;\"\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth style=\"width: 13.2053%; height: 19.5833px;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth style=\"width: 43.5681%; height: 19.5833px;\"\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth style=\"width: 7.56449%; height: 19.5833px;\"\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 31.161%; height: 19.5833px;\"\u003eRGD-Dextran, lyophilized\u003c\/td\u003e\n\u003ctd style=\"width: 13.2053%; height: 19.5833px;\"\u003e2 x 870 µl*\u003c\/td\u003e\n\u003ctd style=\"width: 43.5681%; height: 19.5833px;\"\u003en\/a\u003c\/td\u003e\n\u003ctd style=\"width: 7.56449%; height: 19.5833px;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_RGD-Dextran.pdf?v=1635452072\" title=\"Material Safety Data Sheet (MSDS) 3-D Life RGD-Dextran\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 31.161%; height: 19.5833px;\"\u003eCD-Link, lyophilized\u003c\/td\u003e\n\u003ctd style=\"width: 13.2053%; height: 19.5833px;\"\u003e200 µl*\u003c\/td\u003e\n\u003ctd style=\"width: 43.5681%; height: 19.5833px;\"\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd style=\"width: 7.56449%; height: 19.5833px;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CD-Link.pdf?v=1635452149\" title=\"Material Safety Data Sheet (MSDS) 3-D Life CD-Link\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 31.161%; height: 19.5833px;\"\u003eReconstitution Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 13.2053%; height: 19.5833px;\"\u003e2 x 900 µl\u003c\/td\u003e\n\u003ctd style=\"width: 43.5681%; height: 19.5833px;\"\u003en\/a\u003c\/td\u003e\n\u003ctd style=\"width: 7.56449%; height: 19.5833px;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_Reconstitution_Buffer.pdf?v=1635452204\" title=\"Material Safety Data Sheet (MSDS) 3-D Life Reconstitution Buffer\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 31.161%; height: 19.5833px;\"\u003eWater\u003c\/td\u003e\n\u003ctd style=\"width: 13.2053%; height: 19.5833px;\"\u003e600 µl\u003c\/td\u003e\n\u003ctd style=\"width: 43.5681%; height: 19.5833px;\"\u003en\/a\u003c\/td\u003e\n\u003ctd style=\"width: 7.56449%; height: 19.5833px;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 95.4989%; height: 19.5833px;\" colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Product Datasheet: 3-D Life ToGro\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G94-1.pdf?v=1635451823\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life ToGro\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eChen, Jeng-Chang et al. “Enteric neurospheres retain the capacity to assemble neural networks with motile and metamorphic gliocytes and ganglia.” \u003ci\u003eStem cell research \u0026amp; therapy\u003c\/i\u003e vol. 14,1 290. 5 Oct. 2023, \u003ca href=\"https:\/\/doi.org\/10.1186\/s13287-023-03517-y\" title=\"Chen, Jeng-Chang et al. “Enteric neurospheres retain the capacity to assemble neural networks with motile and metamorphic gliocytes and ganglia.” Stem cell research \u0026amp; therapy vol. 14,1 290. 5 Oct. 2023, doi:10.1186\/s13287-023-03517-y\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1186\/s13287-023-03517-y\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"3-D Life ToGro Hydrogel Kit - Cat. No. G94-1","offer_id":44075609293012,"sku":"G94-1","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/g94_1_2024.png?v=1727200914"},{"product_id":"3-d-life-dextranase","title":"3-D Life Dextranase","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e Dextranase\u003c\/strong\u003e allows the degradation of dextran-based \u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels\u003c\/a\u003e. It contains dextranase from Chaetomium sp., which cleaves glycosidic bonds of dextran. The brown color of the dextranase solution can vary from lot to lot. This does not interfere with the product's quality.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eImportant Note: \u003c\/strong\u003eThe \u003cem\u003e3-D Life\u003c\/em\u003e Dextranase allows the degradation of dextran-based \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels when crosslinked with PEG-Link or CD-Link. It does not allow the degradation when hydrogels are crosslinked with HyLink.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor instructions, please consult General Protocols GP-1 \"Preparation of \u003cem\u003e3-D Life\u003c\/em\u003e Fast Gelling Hydrogels\" or GP-2 \"Preparation of \u003cem\u003e3-D Life\u003c\/em\u003e Slow Gelling Hydrogels\" and the \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels User Guide, which can all be found at the links below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eDissolution of dextran-based hydrogels to recover live or fixed cells without proteolytic degradation. \u003cem\u003e3-D Life\u003c\/em\u003e Dextranase is used at a 1:20 dilution in the culture supernatant to dissolve dextran-based hydrogels. A detailed description of 3D cell culture application is available in the \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels User Guide.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eCAS Registry No.\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e9025-70-1\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eQuantity\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e 500 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eConcentration of reactive groups\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u0026gt;4000 units\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eResources\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003e\u003ca rel=\"noopener\" title=\"3-D Life Dextranase Product Datasheet (PDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_D10-1_2024.09.pdf?v=1727124077\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e Dextranase\u003c\/a\u003e\u003ca title=\"3-D Life Dextranase Product Datasheet\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_D10-1.pdf?v=1635448640\" target=\"_blank\"\u003e\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Material Safety Data Sheet (MSDS): Dextranase\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_dextranase.pdf?v=1635451117\" target=\"_blank\"\u003e\u003cspan\u003e\u003cstrong\u003eMaterial Safety Data Sheet (MSDS):\u003c\/strong\u003e Dextranase\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eOther names:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eEC 3.2.1.11, 1,6-alpha-D-glucan-6-glucanohydrolase, 6-alpha-D-glucan 6-glucanohydrolase, alpha-1,6-D-glucan 6-glucanohydrolase, alpha-1,6-D-glucan-6-glucanohydrolase, Alpha-1,6-glucan-6-glucanohydrolase, alpha-D-1,6-glucan-6-glucanohydrolase, alpha-dextranase, alpha-glucanase, AODex, Dex, Dex2, Dex410, Dex49A, DexA, dextran hydrolase, dextranase, dextranase DL 2, dextranase I, dextranase II, dextransucrase, DL 2, endo-dextranase, endodextranase, extracellular dextranase, Fjoh_4430, LSD1, SmDex, Teth39_0264, TPDex\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH products.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"500 µl - 3-D Life Dextranase - Cat. No. D10-1","offer_id":44075950407892,"sku":"D10-1","price":54.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/d10_1.png?v=1727124129"},{"product_id":"3-d-life-thioglycerol","title":"3-D Life Thioglycerol","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003e3-D Life \u003c\/em\u003eThioglycerol\u003c\/strong\u003e (CAS-No. 96-27-5) can be covalently coupled with its thiol group to SH-reactive groups of Dextran or PVA polymers to modify \u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels\u003c\/a\u003e. For more information and instructions, please consult the \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels User Guide.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eAccessory for 3D cell culture hydrogels. \u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Thioglycerol is used as a negative control in place of peptides to modify \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Hydrogels. If comparative studies at different peptide concentrations are to be made, a corresponding amount of the cell neutral Thioglycerol should be added to ensure equal gel strengths between gels (see Table 4 and 5 in the User Guide).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eQuantity\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e180 µl.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAllows modification of e.g. 9 ml \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel with 0.4 mmol\/L thioglycerol.\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eConcentration of reactive groups\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e20 mmol\/L\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eResources\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Thioglycerol Product Datasheet\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_T10-3.pdf?v=1635449542\" target=\"_blank\"\u003e\u003cspan\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e Thioglycerol\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Thioglycerol Material Safety Data Sheet (MSDS)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_thioglycerol.pdf?v=1635449707\" target=\"_blank\"\u003e\u003cspan\u003e\u003cstrong\u003eMaterial Safety Data Sheet (MSDS):\u003c\/strong\u003e Thioglycerol\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" style=\"font-size: 0.875rem;\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" style=\"font-size: 0.875rem;\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" style=\"font-size: 0.875rem;\" target=\"_blank\"\u003e3-D Life Hydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" style=\"font-size: 0.875rem;\" target=\"_blank\"\u003e3-D Life Hydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eProduct References\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eWang, Zheng et al. “Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.” \u003ci\u003eMaterials (Basel, Switzerland)\u003c\/i\u003e vol. 12,20 3391. 17 Oct. 2019, \u003ca href=\"https:\/\/doi.org\/10.3390\/ma12203391\" title=\"Wang, Zheng et al. “Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.” Materials (Basel, Switzerland) vol. 12,20 3391. 17 Oct. 2019, doi:10.3390\/ma12203391\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.3390\/ma12203391\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eOther names:\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e3-Mercaptopropane-1,2-diol\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH products.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"180 µl - 3-D Life Thioglycerol - Cat. No. T10-3","offer_id":44076040585428,"sku":"T10-3","price":43.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/t10_3_2024.png?v=1727200639"},{"product_id":"3-d-life-10x-cb-buffer-ph-5-5","title":"3-D Life 10x CB-Buffer (pH 5.5)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e 10x CB-Buffer (pH 5.5)\u003c\/strong\u003e is a component of the \u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel\u003c\/a\u003e system. It is a 10x concentrated salt and buffer solution which is diluted to physiological strength when used in the \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel system. It is used for the formation of fast gelling \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels where it provides a cell environment at pH 5.5, which is compatible with most cell types. In conjunction with \u003ca href=\"https:\/\/ilexlife.com\/products\/3-d-life-10x-cb-buffer-ph-7-2\" title=\"3-D Life 10x CB Buffer (pH 7.2)\" rel=\"noopener\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e 10x CB-Buffer (pH 7.2)\u003c\/a\u003e or \u003ca rel=\"noopener\" title=\"3-D Life 10x CB Buffer (pH 7.2) Phenol Red-Free\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-10x-cb-buffer-ph-7-2-phenol-red-free\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e 10x CB Buffer (pH 7.2) Phenol Red-Free\u003c\/a\u003e, it is used to generate pHs of intermediate values to modify the gelation kinetics of fast gelling gels.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor instructions, please consult the General Protocol GP-1 \"Preparation of \u003cem\u003e3-D Life\u003c\/em\u003e Fast Gelling Hydrogels\" and the \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels User Guide, which may be downloaded below.\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eUsed with other components of the \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Hydrogel System to establish 3D cell cultures.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eQuantity\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e600 µl.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e Sufficient for at least 6 ml \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eResources\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Product Datasheet: 10x CB-Buffer (pH 5.5)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_B10-3.pdf?v=1635453002\" target=\"_blank\"\u003e\u003cspan\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 10x CB-Buffer (pH 5.5)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Material Safety Data Sheet (MSDS): 10x CB-Buffer (pH 5.5)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB5.5.pdf?v=1635452874\" target=\"_blank\"\u003e\u003cspan\u003e\u003cstrong\u003eMaterial Safety Data Sheet (MSDS):\u003c\/strong\u003e 10x CB-Buffer (pH 5.5)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eProduct References\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eWang, Zheng et al. “Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.” \u003ci\u003eMaterials (Basel, Switzerland)\u003c\/i\u003e vol. 12,20 3391. 17 Oct. 2019, \u003ca rel=\"noopener\" title=\"Wang, Zheng et al. “Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.” Materials (Basel, Switzerland) vol. 12,20 3391. 17 Oct. 2019, doi:10.3390\/ma12203391\" href=\"https:\/\/doi.org\/10.3390\/ma12203391\" target=\"_blank\"\u003edoi:10.3390\/ma12203391\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eMohammadi Ayenehdeh, Jamal et al. “Introducing a New Experimental Islet Transplantation Model using Biomimetic Hydrogel and a Simple High Yield Islet Isolation Technique.” \u003ci\u003eIranian biomedical journal\u003c\/i\u003e vol. 21,4 (2017): 218-27. \u003ca rel=\"noopener\" title=\"Mohammadi Ayenehdeh, Jamal et al. “Introducing a New Experimental Islet Transplantation Model using Biomimetic Hydrogel and a Simple High Yield Islet Isolation Technique.” Iranian biomedical journal vol. 21,4 (2017): 218-27. doi:10.18869\/acadpub.ibj.21.4.218\" href=\"https:\/\/doi.org\/10.18869\/acadpub.ibj.21.4.218\" target=\"_blank\"\u003edoi:10.18869\/acadpub.ibj.21.4.218\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH products.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"600 µl - 3-D Life 10x CB-Buffer (pH 5.5) - Cat. No. B10-3","offer_id":44075807342804,"sku":"B10-3","price":36.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/b10_3_2024.png?v=1727199856"},{"product_id":"3-d-life-10x-cb-buffer-ph-7-2","title":"3-D Life 10x CB Buffer (pH 7.2)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e 10x CB-Buffer (pH 7.2)\u003c\/strong\u003e is a component of the \u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel\u003c\/a\u003e system. It is a 10x concentrated salt and buffer solution which is diluted to physiological strength when used in the \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel system. It is used for the formation of slow gelling \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels where it provides a cell compatible environment at pH 7.2. In conjunction with \u003ca rel=\"noopener\" title=\"3-D Life 10x CB-Buffer (pH 5.5)\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-10x-cb-buffer-ph-5-5\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e 10x CB-Buffer (pH 5.5)\u003c\/a\u003e it is used to generate pHs of intermediate values to modify the gelation kinetics of fast gelling gels.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor instructions, please consult the General Protocol GP-2 \"Preparation of \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Slow Gelling Hydrogels\" and the \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNOTE: There is a Phenol Red-Free version of this product available for applications in which Phenol Red would interfere with light microscopy analysis: \u003ca rel=\"noopener\" title=\"3-D Life 10x CB Buffer (pH 7.2) Phenol Red-Free\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-10x-cb-buffer-ph-7-2-phenol-red-free\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e 10x CB Buffer (pH 7.2) Phenol Red-Free\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eUsed with other components of the \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Hydrogel System to establish 3D cell cultures.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eQuantity\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e600 µl.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e Sufficient for at least 6 ml \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eResources\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Product Datasheet: 10x CB Buffer (pH 7.2)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_B20-3.pdf?v=1635453626\" target=\"_blank\"\u003e\u003cspan\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 10x CB Buffer (pH 7.2)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Material Safety Data Sheet (MSDS): 10x CB Buffer (pH 7.2)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB7.2.pdf?v=1635453735\" target=\"_blank\"\u003e\u003cspan\u003e\u003cstrong\u003eMaterial Safety Data Sheet (MSDS):\u003c\/strong\u003e 10x CB Buffer (pH 7.2)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eProduct References\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSun, Xue et al. “CloneSeq - Single-cell clonal 3D culture and analysis protocol.” \u003ci\u003eSTAR protocols\u003c\/i\u003e vol. 2,4 100794. 29 Sep. 2021, \u003ca href=\"https:\/\/doi.org\/10.1016\/j.xpro.2021.100794\" title=\"Sun, Xue et al. “CloneSeq - Single-cell clonal 3D culture and analysis protocol.” STAR protocols vol. 2,4 100794. 29 Sep. 2021, doi:10.1016\/j.xpro.2021.100794\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1016\/j.xpro.2021.100794\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eNair, Arya Lekshmi et al. “Parallelizable Microfluidic Platform to Model and Assess In Vitro Cellular Barriers: Technology and Application to Study the Interaction of 3D Tumor Spheroids with Cellular Barriers.” \u003ci\u003eBiosensors\u003c\/i\u003e vol. 11,9 314. 3 Sep. 2021, \u003ca rel=\"noopener\" title=\"Nair, Arya Lekshmi et al. “Parallelizable Microfluidic Platform to Model and Assess In Vitro Cellular Barriers: Technology and Application to Study the Interaction of 3D Tumor Spheroids with Cellular Barriers.” Biosensors vol. 11,9 314. 3 Sep. 2021, doi:10.3390\/bios11090314\" href=\"https:\/\/doi.org\/10.3390\/bios11090314\" target=\"_blank\"\u003edoi:10.3390\/bios11090314\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eGeorgakopoulos, Nikitas et al. “Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.” \u003ci\u003eBMC developmental biology\u003c\/i\u003e vol. 20,1 4. 26 Feb. 2020, \u003ca href=\"https:\/\/doi.org\/10.1186\/s12861-020-0209-5\" title=\"Georgakopoulos, Nikitas et al. “Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.” BMC developmental biology vol. 20,1 4. 26 Feb. 2020, doi:10.1186\/s12861-020-0209-5\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1186\/s12861-020-0209-5\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eFriedrich, Oliver et al. “\u003ci\u003eStretch in Focus\u003c\/i\u003e: 2D Inplane Cell Stretch Systems for Studies of Cardiac Mechano-Signaling.” \u003ci\u003eFrontiers in bioengineering and biotechnology\u003c\/i\u003e vol. 7 55. 27 Mar. 2019, \u003ca rel=\"noopener\" title=\"Friedrich, Oliver et al. “Stretch in Focus: 2D Inplane Cell Stretch Systems for Studies of Cardiac Mechano-Signaling.” Frontiers in bioengineering and biotechnology vol. 7 55. 27 Mar. 2019, doi:10.3389\/fbioe.2019.00055\" href=\"https:\/\/doi.org\/10.3389\/fbioe.2019.00055\" target=\"_blank\"\u003edoi:10.3389\/fbioe.2019.00055\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH products.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"600 µl - 3-D Life 10x CB Buffer (pH 7.2) - Cat. No. B20-3","offer_id":44075781095636,"sku":"B20-3","price":36.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/b20_3_2024.png?v=1727194865"},{"product_id":"3-d-life-rgd-peptide","title":"3-D Life RGD Peptide","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRGD sequences are motifs on extracellular matrix proteins promoting adhesion to cellular integrin receptors. The \u003cem\u003e3-D Life\u003c\/em\u003e RGD Peptide contains a RGD motif and a thiol group on the N-terminus. The thiol group is used to immobilize the peptide on thiol-reactive polymers in \u003ca href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" title=\"3-D Life Biomimetic Hydrogels\" rel=\"noopener\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels\u003c\/a\u003e. The immobilized peptide promotes adhesion of cells carrying the appropriate integrin receptor.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor instructions, please consult General Protocols GP-1 \"Preparation of \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Fast Gelling Hydrogels\" or GP-2 \"Preparation of \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Slow Gelling Hydrogels\" and the \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cp\u003eModification of \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels with cell-adhesive properties. 3D cell culture of cells requiring a cell-adhesive matrix based on the RGD motif.\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong\u003eCat. No. 09-P-001\u003c\/strong\u003e: \u003c\/span\u003e1 µmol\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCat. No. P10-3:\u003c\/strong\u003e 3x 1 µmol\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eCat. No. 09-P-001\u003c\/strong\u003e\u003cspan\u003e:\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eRGD Peptide, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 µmol\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_RGD.pdf?v=1635454780\" title=\"Material Safety Data Sheet (MSDS) 3-D Life RGD Peptide\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e600 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCat. No. P10-3:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eRGD Peptide, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e3 x 1 µmol\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life RGD Peptide\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_RGD.pdf?v=1635454780\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e600 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003ePeptide sequence\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eAcetyl-Cys-Doa*-Doa-Gly-Arg-Gly-Asp-Ser-Pro-NH2\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e*: Doa:8-amino-3,6-dioxaoctanoic acid\u003c\/p\u003e\n\u003ch2\u003eMolar mass\u003c\/h2\u003e\n\u003cp\u003e1022 g\/mol\u003c\/p\u003e\n\u003ch2\u003ePurity\u003c\/h2\u003e\n\u003cp\u003e\u0026gt;90% (HPLC)\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Product Datasheet: 3-D Life RGD Peptide (1 µmol)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_09-P-001.pdf?v=1635454990\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life RGD Peptide (\u003cspan data-mce-fragment=\"1\"\u003e1 µmol)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Product Datasheet: 3-D Life RGD Peptide (3x 1 µmol)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_P10-3.pdf?v=1635540313\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life RGD Peptide (3x \u003cspan data-mce-fragment=\"1\"\u003e1 µmol)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" title=\"3-D Life Biomimetic Hydrogels Page\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/p_3_co-culture_tumor_and_stroma_cells.pdf?v=1635455197\" title=\"Application Protocol: Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels\" target=\"_blank\"\u003e\u003cstrong\u003eApplication Protocol:\u003c\/strong\u003e Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/app_note_3_-_Co-culture_of_tumor_and_stroma_cells_in_3-D_Life_Hydrogels.pdf?v=1635455437\" title=\"Application Note: Co-culture of tumor and stroma cells in 3-D Life Hydrogels\" target=\"_blank\"\u003e\u003cstrong\u003eApplication Note:\u003c\/strong\u003e Co-culture of tumor and stroma cells in 3-D Life Hydrogels\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Application_Note_1_-_Epithelial_Cyst_Formation_in_3-D_Life_Hydrogels.pdf?v=1635455275\" title=\"Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels\" target=\"_blank\"\u003e\u003cstrong\u003eApplication Note:\u003c\/strong\u003e Epithelial Cyst Formation in 3-D Life Hydrogels\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/p_1_cyst_formation_-_MDCK_cyst_formation_in_3-D_Life_Hydrogels.pdf?v=1635455325\" title=\"Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels\" target=\"_blank\"\u003e\u003cstrong\u003eApplication Protocol:\u003c\/strong\u003e MDCK Cyst Formation in 3-D Life Hydrogels\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Application_Note_2_-_Fibroblast_Spreading_in_Cell-Degradable_3-D_Life_Hydrogels.pdf?v=1635455373\" title=\"Application Note: Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels\" target=\"_blank\"\u003e\u003cstrong\u003eApplication Note:\u003c\/strong\u003e Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGuenther, Carla. “Stiffness regulates dendritic cell and macrophage subtype development and increased stiffness induces a tumor-associated macrophage phenotype in cancer co-cultures.” \u003ci\u003eFrontiers in immunology\u003c\/i\u003e vol. 15 1434030. 15 Aug. 2024, \u003ca href=\"https:\/\/doi.org\/10.3389\/fimmu.2024.1434030\" title=\"Guenther, Carla. “Stiffness regulates dendritic cell and macrophage subtype development and increased stiffness induces a tumor-associated macrophage phenotype in cancer co-cultures.” Frontiers in immunology vol. 15 1434030. 15 Aug. 2024, doi:10.3389\/fimmu.2024.1434030\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.3389\/fimmu.2024.1434030\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eNarasimhan, Badri Narayanan, and Stephanie I Fraley. “Degradability tunes ECM stress relaxation and cellular mechanics.” \u003ci\u003ebioRxiv : the preprint server for biology\u003c\/i\u003e 2024.07.28.605514. 29 Jul. 2024, \u003ca rel=\"noopener\" title=\"Narasimhan, Badri Narayanan, and Stephanie I Fraley. “Degradability tunes ECM stress relaxation and cellular mechanics.” bioRxiv : the preprint server for biology 2024.07.28.605514. 29 Jul. 2024, doi:10.1101\/2024.07.28.605514. Preprint.\" href=\"https:\/\/doi.org\/10.1101\/2024.07.28.605514\" target=\"_blank\"\u003edoi:10.1101\/2024.07.28.605514\u003c\/a\u003e. Preprint.\u003c\/li\u003e\n\u003cli\u003eCipriano, Madalena et al. “Human immunocompetent choroid-on-chip: a novel tool for studying ocular effects of biological drugs.” \u003ci\u003eCommunications biology\u003c\/i\u003e vol. 5,1 52. 13 Jan. 2022, \u003ca href=\"https:\/\/doi.org\/10.1038\/s42003-021-02977-3\" title=\"Cipriano, Madalena et al. “Human immunocompetent choroid-on-chip: a novel tool for studying ocular effects of biological drugs.” Communications biology vol. 5,1 52. 13 Jan. 2022, doi:10.1038\/s42003-021-02977-3\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1038\/s42003-021-02977-3\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKuehlbach, Claudia et al. “Recapitulating the Angiogenic Switch in a Hydrogel-Based 3D In Vitro Tumor-Stroma Model.” \u003ci style=\"font-size: 0.875rem;\"\u003eBioengineering (Basel, Switzerland)\u003c\/i\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e vol. 8,11 186. 15 Nov. 2021, \u003ca href=\"https:\/\/doi.org\/10.3390\/bioengineering8110186\" title=\"Kuehlbach, Claudia et al. “Recapitulating the Angiogenic Switch in a Hydrogel-Based 3D In Vitro Tumor-Stroma Model.” Bioengineering (Basel, Switzerland) vol. 8,11 186. 15 Nov. 2021, doi:10.3390\/bioengineering8110186\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.3390\/bioengineering8110186\u003c\/a\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003eSun, Xue et al. “CloneSeq - Single-cell clonal 3D culture and analysis protocol.” \u003ci\u003eSTAR protocols\u003c\/i\u003e vol. 2,4 100794. 29 Sep. 2021, \u003ca rel=\"noopener\" title=\"Sun, Xue et al. “CloneSeq - Single-cell clonal 3D culture and analysis protocol.” STAR protocols vol. 2,4 100794. 29 Sep. 2021, doi:10.1016\/j.xpro.2021.100794\" href=\"https:\/\/doi.org\/10.1016\/j.xpro.2021.100794\" target=\"_blank\"\u003edoi:10.1016\/j.xpro.2021.100794\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eNair, Arya Lekshmi et al. “Parallelizable Microfluidic Platform to Model and Assess In Vitro Cellular Barriers: Technology and Application to Study the Interaction of 3D Tumor Spheroids with Cellular Barriers.” \u003ci\u003eBiosensors\u003c\/i\u003e vol. 11,9 314. 3 Sep. 2021, \u003ca rel=\"noopener\" title=\"Nair, Arya Lekshmi et al. “Parallelizable Microfluidic Platform to Model and Assess In Vitro Cellular Barriers: Technology and Application to Study the Interaction of 3D Tumor Spheroids with Cellular Barriers.” Biosensors vol. 11,9 314. 3 Sep. 2021, doi:10.3390\/bios11090314\" href=\"https:\/\/doi.org\/10.3390\/bios11090314\" target=\"_blank\"\u003edoi:10.3390\/bios11090314\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eRoy, Tanaya et al. “Anti-VEGF-R2 Aptamer and RGD Peptide Synergize in a Bifunctional Hydrogel for Enhanced Angiogenic Potential.” \u003ci\u003eMacromolecular bioscience\u003c\/i\u003e vol. 21,2 (2021): e2000337. \u003ca href=\"https:\/\/doi.org\/10.1002\/mabi.202000337\" title=\"Roy, Tanaya et al. “Anti-VEGF-R2 Aptamer and RGD Peptide Synergize in a Bifunctional Hydrogel for Enhanced Angiogenic Potential.” Macromolecular bioscience vol. 21,2 (2021): e2000337. doi:10.1002\/mabi.202000337\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1002\/mabi.202000337\u003c\/a\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003eGeorgakopoulos, Nikitas et al. “Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.” \u003ci style=\"font-size: 0.875rem;\"\u003eBMC developmental biology\u003c\/i\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e vol. 20,1 4. 26 Feb. 2020, \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1186\/s12861-020-0209-5\" title=\"Georgakopoulos, Nikitas et al. “Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.” BMC developmental biology vol. 20,1 4. 26 Feb. 2020, doi:10.1186\/s12861-020-0209-5\" rel=\"noopener\" style=\"font-size: 0.875rem;\" target=\"_blank\"\u003edoi:10.1186\/s12861-020-0209-5\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eWang, Zheng et al. “Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.” \u003ci style=\"font-size: 0.875rem;\"\u003eMaterials (Basel, Switzerland)\u003c\/i\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e vol. 12,20 3391. 17 Oct. 2019, \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Wang, Zheng et al. “Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.” Materials (Basel, Switzerland) vol. 12,20 3391. 17 Oct. 2019, doi:10.3390\/ma12203391\" href=\"https:\/\/doi.org\/10.3390\/ma12203391\" style=\"font-size: 0.875rem;\" target=\"_blank\"\u003edoi:10.3390\/ma12203391\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eFriedrich, Oliver et al. “\u003ci\u003eStretch in Focus\u003c\/i\u003e: 2D Inplane Cell Stretch Systems for Studies of Cardiac Mechano-Signaling.” \u003ci\u003eFrontiers in bioengineering and biotechnology\u003c\/i\u003e vol. 7 55. 27 Mar. 2019, \u003ca href=\"https:\/\/doi.org\/10.3389\/fbioe.2019.00055\" title=\"Friedrich, Oliver et al. “Stretch in Focus: 2D Inplane Cell Stretch Systems for Studies of Cardiac Mechano-Signaling.” Frontiers in bioengineering and biotechnology vol. 7 55. 27 Mar. 2019, doi:10.3389\/fbioe.2019.00055\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.3389\/fbioe.2019.00055\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eGrobe, Hanna et al. “A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions.” \u003ci\u003ePloS one\u003c\/i\u003e vol. 13,3 e0194716. 26 Mar. 2018, \u003ca rel=\"noopener\" title=\"Grobe, Hanna et al. “A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions.” PloS one vol. 13,3 e0194716. 26 Mar. 2018, doi:10.1371\/journal.pone.0194716\" href=\"https:\/\/doi.org\/10.1371\/journal.pone.0194716\" target=\"_blank\"\u003edoi:10.1371\/journal.pone.0194716\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eNugraha, Bramasta et al. “Monitoring and manipulating cellular crosstalk during kidney fibrosis inside a 3D in vitro co-culture.” \u003ci\u003eScientific reports\u003c\/i\u003e vol. 7,1 14490. 3 Nov. 2017, \u003ca href=\"https:\/\/doi.org\/10.1038\/s41598-017-12683-y\" title=\"Nugraha, Bramasta et al. “Monitoring and manipulating cellular crosstalk during kidney fibrosis inside a 3D in vitro co-culture.” Scientific reports vol. 7,1 14490. 3 Nov. 2017, doi:10.1038\/s41598-017-12683-y\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1038\/s41598-017-12683-y\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eGrikscheit, Katharina et al. “Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1.” \u003ci\u003eThe Journal of cell biology\u003c\/i\u003e vol. 209,3 (2015): 367-76. \u003ca rel=\"noopener\" title=\"Grikscheit, Katharina et al. “Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1.” The Journal of cell biology vol. 209,3 (2015): 367-76. doi:10.1083\/jcb.201412015\" href=\"https:\/\/doi.org\/10.1083\/jcb.201412015\" target=\"_blank\"\u003edoi:10.1083\/jcb.201412015\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"1 µmol - 3-D Life RGD Peptide - Cat. No. 09-P-001","offer_id":41208444387480,"sku":"09-P-001","price":181.0,"currency_code":"USD","in_stock":true},{"title":"3 x 1 µmol - 3-D Life RGD Peptide - Cat. No. P10-3","offer_id":41208455430296,"sku":"P10-3","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/09-p-001_2024.png?v=1727211347"},{"product_id":"3-d-life-scrambled-rgd-peptide","title":"3-D Life Scrambled RGD Peptide","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRGD sequences are motifs on extracellular matrix proteins promoting adhesion to cellular integrin receptors. The \u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Scrambled RGD Peptide\u003c\/strong\u003e contains a scrambled RGD motif and a thiol group on the N-terminus. The thiol group is used to immobilize the peptide on maleimide polymers in \u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels\u003c\/a\u003e. The immobilized peptide is used in control experiments to determine the specificity of cell adhesion caused by a genuine RGD peptide (e.g. \u003ca title=\"3-D Life RGD Peptide Product Page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-rgd-peptide\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e RGD Peptide\u003c\/a\u003e).\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor instructions, please consult General Protocols GP-1 \"Preparation of \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Fast Gelling Hydrogels\" or GP-2 \"Preparation of \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Slow Gelling Hydrogels\" and the \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e3D cell culture: Covalent attachment to thiol-reactive polymer in \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Hydrogels. For control experiments to determine specificity of cell adhesion to RGD Peptide.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eCat. No. 09-P-003:\u003c\/strong\u003e 1 µmol\u003c\/p\u003e\n\u003cp\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCat. No. P11-3: \u003c\/strong\u003e3x 1 µmol\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eCat. No. 09-P-003:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eScrambled RGD Peptide, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e1 µmol\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Scrambled RGD Peptide\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_scrambled_RGD.pdf?v=1635456470\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e600 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCat. No. P11-3:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eScrambled RGD Peptide, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e3 x 1 µmol\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_scrambled_RGD.pdf?v=1635456470\" title=\"Material Safety Data Sheet (MSDS) 3-D Life Scrambled RGD Peptide\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e600 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePeptide sequence\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eAcetyl-Cys-Doa*-Doa-Gly-Arg-Asp-Gly-Ser-Pro-NH2\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e*: Doa:8-amino-3,6-dioxaoctanoic acid\u003c\/p\u003e\n\u003ch2\u003eMolar mass\u003c\/h2\u003e\n\u003cp\u003e1022 g\/mol\u003c\/p\u003e\n\u003ch2\u003ePurity\u003c\/h2\u003e\n\u003cp\u003e\u0026gt;90% (HPLC)\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Product Datasheet: 3-D Life Scrambled RGD Peptide (1 µmol)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_09-P-003.pdf?v=1635456636\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life Scrambled RGD Peptide (\u003cspan data-mce-fragment=\"1\"\u003e1 µmol)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Product Datasheet: 3-D Life Scrambled RGD Peptide (3x 1 µmol)\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_P11-3.pdf?v=1635540619\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life Scrambled RGD Peptide (3x \u003cspan data-mce-fragment=\"1\"\u003e1 µmol)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Application_Note_1_-_Epithelial_Cyst_Formation_in_3-D_Life_Hydrogels.pdf?v=1635455275\" target=\"_blank\"\u003e\u003cstrong\u003eApplication Note:\u003c\/strong\u003e Epithelial Cyst Formation in 3-D Life Hydrogels\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/p_1_cyst_formation_-_MDCK_cyst_formation_in_3-D_Life_Hydrogels.pdf?v=1635455325\" target=\"_blank\"\u003e\u003cstrong\u003eApplication Protocol:\u003c\/strong\u003e MDCK Cyst Formation in 3-D Life Hydrogels\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"1 µmol - 3-D Life Scrambled RGD Peptide - Cat. No. 09-P-003","offer_id":41208490918040,"sku":"09-P-003","price":181.0,"currency_code":"USD","in_stock":true},{"title":"3 x 1 µmol - 3-D Life Scrambled RGD Peptide - Cat. No. P11-3","offer_id":41208490950808,"sku":"P11-3","price":420.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/09-p-003_2024.png?v=1727292441"},{"product_id":"3-d-life-pva-peg-hydrogel-sg-kit","title":"3-D Life PVA-PEG Hydrogel SG Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe \u003c\/span\u003e\u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003c\/strong\u003e\u003cspan\u003e\u003cstrong\u003e PVA-PEG Hydrogel SG Kit\u003c\/strong\u003e provides reagents for setting up slow gelling, cell-compatible \u003ca href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" title=\"3-D Life Biomimetic Hydrogels\" rel=\"noopener\" target=\"_blank\"\u003e\u003cem\u003e3-D Life \u003c\/em\u003eHydrogels\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIts major components are the polymeric ­SG-PVA and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. Prior to the crosslinking step, peptides (e.g. \u003ca title=\"3-D Life RGD Peptide Product Page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-rgd-peptide\" target=\"_blank\"\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e RGD Peptide, Cat. No. 09-P-001\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e) can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor more information and instructions, please consult the General Protocol GP-2 \"Preparation of \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Slow Gelling Hydrogels\" and the \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e3D cell cultures\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eAllows formation of up to 2 ml \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Hydrogel depending on the stiffness of the gel.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSG-PVA\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e30 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life SG-PVA\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_SG-PVA.pdf?v=1635518272\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003ePEG-Link, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl*\u003c\/td\u003e\n\u003ctd\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life PEG-Link\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_PEG-Link.pdf?v=1635518317\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10x CB Buffer (pH 7.2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CB Buffer pH 7.2\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB7.2.pdf?v=1635453735\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e2 x 1500 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Product Datasheet: 3-D Life PVA-PEG Hydrogel SG Kit\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G82-1.pdf?v=1635518700\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life PVA-PEG Hydrogel SG Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNarasimhan, Badri Narayanan, and Stephanie I Fraley. “Degradability tunes ECM stress relaxation and cellular mechanics.” \u003ci\u003ebioRxiv : the preprint server for biology\u003c\/i\u003e 2024.07.28.605514. 29 Jul. 2024, \u003ca href=\"https:\/\/doi.org\/10.1101\/2024.07.28.605514\" title=\"Narasimhan, Badri Narayanan, and Stephanie I Fraley. “Degradability tunes ECM stress relaxation and cellular mechanics.” bioRxiv : the preprint server for biology 2024.07.28.605514. 29 Jul. 2024, doi:10.1101\/2024.07.28.605514. Preprint.\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1101\/2024.07.28.605514\u003c\/a\u003e. Preprint.\u003c\/li\u003e\n\u003cli\u003eFriedrich, Oliver et al. “\u003ci\u003eStretch in Focus\u003c\/i\u003e: 2D Inplane Cell Stretch Systems for Studies of Cardiac Mechano-Signaling.” \u003ci\u003eFrontiers in bioengineering and biotechnology\u003c\/i\u003e vol. 7 55. 27 Mar. 2019, \u003ca rel=\"noopener\" title=\"Friedrich, Oliver et al. “Stretch in Focus: 2D Inplane Cell Stretch Systems for Studies of Cardiac Mechano-Signaling.” Frontiers in bioengineering and biotechnology vol. 7 55. 27 Mar. 2019, doi:10.3389\/fbioe.2019.00055\" href=\"https:\/\/doi.org\/10.3389\/fbioe.2019.00055\" target=\"_blank\"\u003edoi:10.3389\/fbioe.2019.00055\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"3-D Life PVA-PEG Hydrogel SG Kit - Cat. No. G82-1","offer_id":44075976392916,"sku":"G82-1","price":145.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/g82_1_528102186.png?v=1729790644"},{"product_id":"3-d-life-pva-cd-hydrogel-sg-kit","title":"3-D Life PVA-CD Hydrogel SG Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e PVA-CD Hydrogel SG Kit\u003c\/strong\u003e provides reagents for setting up slow gelling, cell-compatible \u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cem\u003e3-D Life \u003c\/em\u003eHydrogels\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003eIts major components are polymeric SG-PVA and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9. It allows cells to spread and migrate within the hydrogel if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003ePrior to the cross-linking step, peptides (e.g. \u003ca href=\"https:\/\/ilexlife.com\/products\/3-d-life-rgd-peptide\" title=\"3-D Life RGD Peptide Product Page\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e RGD Peptide, Cat. No. 09-P-001\u003c\/a\u003e) can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor more information and instructions, please consult the General Protocol GP-2 \"Preparation of \u003cem\u003e3-D Life\u003c\/em\u003e Slow Gelling Hydrogels\" and the \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e3D cell cultures\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003eAllows formation of up to 2 ml \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel depending on the stiffness of the gel.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSG-PVA\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e30 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life SG-PVA\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_SG-PVA.pdf?v=1635518272\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eCD-Link, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl*\u003c\/td\u003e\n\u003ctd\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CD-Link\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CD-Link.pdf?v=1635452149\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10x CB Buffer (pH 7.2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CB Buffer pH 7.2\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB7.2.pdf?v=1635453735\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e2 x 1500 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e Resources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Product Datasheet: 3-D Life PVA-CD Hydrogel SG Kit\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G83-1.pdf?v=1635519916\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life PVA-CD Hydrogel SG Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"3-D Life PVA-CD Hydrogel SG Kit - Cat. No. G83-1","offer_id":44075966070996,"sku":"G83-1","price":333.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/g83_1_1626727654.png?v=1729790046"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/collections\/cell_culture2_617aafc7-fc80-46c8-99d9-9432090982ef.jpg?v=1664920289","url":"https:\/\/ilexlife.com\/collections\/cell-culture-reagents\/hyaluronic-acid.oembed","provider":"Ilex Life Sciences","version":"1.0","type":"link"}