{"title":"3-D Life Ready-To-Design Hydrogel Kits for 3D Cell Culture","description":"\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003eThe \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels: Cell Environment Design at Your Fingertips\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" data-mce-href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cstrong\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e\u003c\/strong\u003e\u003c\/a\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels: Cell Environment Design at Your Fingertips\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" data-mce-href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cstrong\u003e Hydrogels\u003c\/strong\u003e\u003c\/a\u003e technology by Cellendes is an integrated system for the generation of biomimetic hydrogels used in 3D cell culture. The \u003c\/span\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e\u003cspan data-mce-fragment=\"1\"\u003e system consists of two polymers that form a hydrogel by covalent crosslinking at ambient temperature. The chemical reaction is non-toxic to cells and occurs rapidly, preventing cell settlement before the gel is formed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe \u003cem\u003e3-D Life \u003c\/em\u003eReady-To-Design Hydrogel Kits contain a thiol-reactive polymer, a crosslinker and a 10x concentrated salt and buffer solution. Cell adhesion peptides, Dextranase for gel dissolution and other gel modifying reagents are available as separate products (see products Adhesion Peptides and Accessories).\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/collections\/3-d-life-pva-hydrogel-kits\" title=\"3-D Life PVA Hydrogel Kits for 3D Cell Culture\" rel=\"null noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cstrong\u003ePVA Hydrogel Kits\u003c\/strong\u003e\u003c\/a\u003e: \u003cspan data-mce-fragment=\"1\"\u003ePVA (polyvinyl alcohol) is a biochemically stable polymer providing long lasting hydrogel stability for long-term cell cultures.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/3-d-life-dextran-hydrogel-kits\" title=\"3-D Life Dextran Hydrogel Kits for 3D Cell Culture\" rel=\"null noopener\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cstrong\u003eDextran Hydrogel Kits\u003c\/strong\u003e\u003c\/a\u003e: Dextran Hydrogel Kits contain thiol-reactive dextran. Dextran hydrogels can be dissolved by \u003ca href=\"https:\/\/ilexlife.com\/products\/3-d-life-dextranase\" title=\"3-D Life Dextranase\" aria-describedby=\"a11y-new-window-message\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/ilexlife.com\/products\/3-d-life-dextranase\" target=\"_blank\"\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e Dextranase\u003c\/a\u003e for a protease-free cell recovery when crosslinked with PEG-Link or CD-Link. Dextran hydrogels crosslinked with HyLink (Dextran-HA Hydrogel Kit) cannot be dissolved by \u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e Dextranase.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/3-d-life-hyaluronic-acid-hydrogel-kits\" title=\"3-D Life Hyaluronic Acid Hydrogel Kits for 3D Cell Culture\" rel=\"noopener noreferrer\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cstrong\u003eHyaluronic Acid Hydrogel Kits\u003c\/strong\u003e\u003c\/a\u003e: Hyaluronic Acid Hydrogel Kits contain thiol-modified hyaluronic acid (HA) as a crosslinker.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eHydrogel composition and properties are indicated in their names for easy hydrogel selection:\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePVA or Dextran:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ePVA (polyvinyl alcohol) is a biochemically stable polymer providing long lasting hydrogel stability for long-term cell cultures. Dextran hydrogels can be dissolved by Dextranase (see Accessories) for a protease-free cell recovery.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCD or PEG:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eChoose between a cell-degradable (CD) crosslinker and a non-degradable (PEG) crosslinker.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFG or SG:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eChoose between fast gelling (FG) and slow gelling (SG) hydrogels to adapt the set-up of hydrogels to your experimental requirements.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHyaluronic acid:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eSelect hydrogels crosslinked with hyaluronic acid.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH 3-D Life products.\u003c\/em\u003e\u003c\/p\u003e","products":[{"product_id":"3-d-life-pva-peg-hydrogel-sg-kit","title":"3-D Life PVA-PEG Hydrogel SG Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe \u003c\/span\u003e\u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003c\/strong\u003e\u003cspan\u003e\u003cstrong\u003e PVA-PEG Hydrogel SG Kit\u003c\/strong\u003e provides reagents for setting up slow gelling, cell-compatible \u003ca href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" title=\"3-D Life Biomimetic Hydrogels\" rel=\"noopener\" target=\"_blank\"\u003e\u003cem\u003e3-D Life \u003c\/em\u003eHydrogels\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIts major components are the polymeric ­SG-PVA and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. Prior to the crosslinking step, peptides (e.g. \u003ca title=\"3-D Life RGD Peptide Product Page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-rgd-peptide\" target=\"_blank\"\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e RGD Peptide, Cat. No. 09-P-001\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e) can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor more information and instructions, please consult the General Protocol GP-2 \"Preparation of \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Slow Gelling Hydrogels\" and the \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e3D cell cultures\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eAllows formation of up to 2 ml \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Hydrogel depending on the stiffness of the gel.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSG-PVA\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e30 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life SG-PVA\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_SG-PVA.pdf?v=1635518272\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003ePEG-Link, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl*\u003c\/td\u003e\n\u003ctd\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life PEG-Link\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_PEG-Link.pdf?v=1635518317\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10x CB Buffer (pH 7.2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CB Buffer pH 7.2\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB7.2.pdf?v=1635453735\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e2 x 1500 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Product Datasheet: 3-D Life PVA-PEG Hydrogel SG Kit\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G82-1.pdf?v=1635518700\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life PVA-PEG Hydrogel SG Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNarasimhan, Badri Narayanan, and Stephanie I Fraley. “Degradability tunes ECM stress relaxation and cellular mechanics.” \u003ci\u003ebioRxiv : the preprint server for biology\u003c\/i\u003e 2024.07.28.605514. 29 Jul. 2024, \u003ca href=\"https:\/\/doi.org\/10.1101\/2024.07.28.605514\" title=\"Narasimhan, Badri Narayanan, and Stephanie I Fraley. “Degradability tunes ECM stress relaxation and cellular mechanics.” bioRxiv : the preprint server for biology 2024.07.28.605514. 29 Jul. 2024, doi:10.1101\/2024.07.28.605514. Preprint.\" rel=\"noopener\" target=\"_blank\"\u003edoi:10.1101\/2024.07.28.605514\u003c\/a\u003e. Preprint.\u003c\/li\u003e\n\u003cli\u003eFriedrich, Oliver et al. “\u003ci\u003eStretch in Focus\u003c\/i\u003e: 2D Inplane Cell Stretch Systems for Studies of Cardiac Mechano-Signaling.” \u003ci\u003eFrontiers in bioengineering and biotechnology\u003c\/i\u003e vol. 7 55. 27 Mar. 2019, \u003ca rel=\"noopener\" title=\"Friedrich, Oliver et al. “Stretch in Focus: 2D Inplane Cell Stretch Systems for Studies of Cardiac Mechano-Signaling.” Frontiers in bioengineering and biotechnology vol. 7 55. 27 Mar. 2019, doi:10.3389\/fbioe.2019.00055\" href=\"https:\/\/doi.org\/10.3389\/fbioe.2019.00055\" target=\"_blank\"\u003edoi:10.3389\/fbioe.2019.00055\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"3-D Life PVA-PEG Hydrogel SG Kit - Cat. No. G82-1","offer_id":44075976392916,"sku":"G82-1","price":145.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/g82_1_528102186.png?v=1729790644"},{"product_id":"3-d-life-pva-cd-hydrogel-sg-kit","title":"3-D Life PVA-CD Hydrogel SG Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e PVA-CD Hydrogel SG Kit\u003c\/strong\u003e provides reagents for setting up slow gelling, cell-compatible \u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cem\u003e3-D Life \u003c\/em\u003eHydrogels\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003eIts major components are polymeric SG-PVA and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9. It allows cells to spread and migrate within the hydrogel if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003ePrior to the cross-linking step, peptides (e.g. \u003ca href=\"https:\/\/ilexlife.com\/products\/3-d-life-rgd-peptide\" title=\"3-D Life RGD Peptide Product Page\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e RGD Peptide, Cat. No. 09-P-001\u003c\/a\u003e) can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor more information and instructions, please consult the General Protocol GP-2 \"Preparation of \u003cem\u003e3-D Life\u003c\/em\u003e Slow Gelling Hydrogels\" and the \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e3D cell cultures\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003eAllows formation of up to 2 ml \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel depending on the stiffness of the gel.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSG-PVA\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e30 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life SG-PVA\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_SG-PVA.pdf?v=1635518272\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eCD-Link, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl*\u003c\/td\u003e\n\u003ctd\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CD-Link\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CD-Link.pdf?v=1635452149\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10x CB Buffer (pH 7.2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CB Buffer pH 7.2\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB7.2.pdf?v=1635453735\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e2 x 1500 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e Resources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Product Datasheet: 3-D Life PVA-CD Hydrogel SG Kit\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G83-1.pdf?v=1635519916\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life PVA-CD Hydrogel SG Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"3-D Life PVA-CD Hydrogel SG Kit - Cat. No. G83-1","offer_id":44075966070996,"sku":"G83-1","price":333.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/g83_1_1626727654.png?v=1729790046"},{"product_id":"3-d-life-dextran-peg-hydrogel-sg-kit","title":"3-D Life Dextran-PEG Hydrogel SG Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe \u003c\/span\u003e\u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003c\/strong\u003e\u003cspan\u003e\u003cstrong\u003e Dextran-PEG Hydrogel SG Kit\u003c\/strong\u003e provides reagents for setting up slow gelling, cell-compatible \u003ca href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" title=\"3-D Life Biomimetic Hydrogels\" rel=\"noopener\" target=\"_blank\"\u003e\u003cem\u003e3-D Life \u003c\/em\u003eHydrogels\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIts major components are SG-Dextran and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with thiol-reactive groups on SG-Dextran, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrior to the crosslinking step, cell adhesion peptides (e.g. \u003ca title=\"3-D Life RGD Peptide Product Page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-rgd-peptide\" target=\"_blank\"\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e RGD Peptide, Cat. No. 09-P-001\u003c\/a\u003e) can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell-adhesive matrix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eDextran hydrogels crosslinked with PEG-Link can be dissolved by the addition of dextranase (\u003ca title=\"3-D Life Dextranase Product Page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-dextranase\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Dextranase, Cat. No. D10-1\u003c\/a\u003e), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor more information and instructions, please consult the General Protocol GP-2 \"Preparation of \u003cem\u003e3-D Life\u003c\/em\u003e Slow Gelling Hydrogels\" and the \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e3D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels.\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003eAllows formation of up to 2 ml \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel depending on the stiffness of the gel.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSG-Dextran\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e30 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_SG-Dextran.pdf?v=1635520621\" title=\"Material Safety Data Sheet (MSDS) 3-D Life SG-Dextran\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003ePEG-Link, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl*\u003c\/td\u003e\n\u003ctd\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_PEG-Link.pdf?v=1635518317\" title=\"Material Safety Data Sheet (MSDS) 3-D Life PEG-Link\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10x CB Buffer (pH 7.2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB7.2.pdf?v=1635453735\" title=\"Material Safety Data Sheet (MSDS) 3-D Life CB Buffer pH 7.2\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e2 x 1500 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G92-1_Ilex.pdf?v=1729793039\" title=\"Product Datasheet: 3-D Life Dextran-PEG Hydrogel SG Kit\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life Dextran-PEG Hydrogel SG Kit\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G92-1.pdf?v=1635520802\" title=\"Product Datasheet: 3-D Life Dextran-PEG Hydrogel SG Kit\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" title=\"3-D Life Biomimetic Hydrogels Page\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMilitaru, Ioana V et al. “New panel of biomarkers to discriminate between amelanotic and melanotic metastatic melanoma.” \u003ci\u003eFrontiers in oncology\u003c\/i\u003e vol. 12 1061832. 26 Jan. 2023, \u003ca rel=\"noopener\" title=\"Militaru, Ioana V et al. “New panel of biomarkers to discriminate between amelanotic and melanotic metastatic melanoma.” Frontiers in oncology vol. 12 1061832. 26 Jan. 2023, doi:10.3389\/fonc.2022.1061832\" href=\"https:\/\/doi.org\/10.3389\/fonc.2022.1061832\" target=\"_blank\"\u003edoi:10.3389\/fonc.2022.1061832\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"3-D Life Dextran-PEG Hydrogel SG Kit - Cat. No. G92-1","offer_id":44075937071316,"sku":"G92-1","price":153.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/fg92_1.png?v=1729792765"},{"product_id":"3-d-life-dextran-cd-hydrogel-sg-kit","title":"3-D Life Dextran-CD Hydrogel SG Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe \u003c\/span\u003e\u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003c\/strong\u003e\u003cspan\u003e\u003cstrong\u003e Dextran-CD Hydrogel SG Kit\u003c\/strong\u003e provides reagents for setting up slow gelling, cell-compatible \u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIts major components are SG-Dextran and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on SG-Dextran, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9. It allows cells to spread and migrate within the hydrogel if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrior to the crosslinking step, cell adhesion peptides (e.g. \u003ca href=\"https:\/\/ilexlife.com\/products\/3-d-life-rgd-peptide\" title=\"3-D Life RGD Peptide Product Page\" target=\"_blank\"\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e RGD Peptide, Cat. No. 09-P-001\u003c\/a\u003e) can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell-adhesive matrix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eSG-Dextran hydrogels crosslinked with CD-Link can be dissolved by the addition of dextranase (\u003ca href=\"https:\/\/ilexlife.com\/products\/3-d-life-dextranase\" title=\"3-D Life Dextranase Product Page\" target=\"_blank\"\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e Dextranase, Cat. No. D10-1\u003c\/a\u003e), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor more information and instructions, please consult the General Protocol GP-2 \"Preparation of \u003cem\u003e3-D Life\u003c\/em\u003e Slow Gelling Hydrogels\" and the \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e3D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels.\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003eAllows formation of up to 2 ml \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel depending on the stiffness of the gel.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSG-Dextran\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e30 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life SG-Dextran\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_SG-Dextran.pdf?v=1635520621\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eCD-Link, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl*\u003c\/td\u003e\n\u003ctd\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CD-Link\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CD-Link.pdf?v=1635452149\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10x CB Buffer (pH 7.2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CB Buffer pH 7.2\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB7.2.pdf?v=1635453735\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e2 x 1500 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e Resources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"Product Datasheet: 3-D Life Dextran-CD Hydrogel SG Kit\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G93-1_2024.pdf?v=1729705159\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life Dextran-CD Hydrogel SG Kit\u003c\/a\u003e\u003ca title=\"Product Datasheet: 3-D Life Dextran-CD Hydrogel SG Kit\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G93-1.pdf?v=1635523602\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"3-D Life Dextran-CD Hydrogel SG Kit - Cat. No. G93-1","offer_id":44075903418580,"sku":"G93-1","price":332.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/fg93_1.png?v=1729704842"},{"product_id":"3-d-life-dextran-ha-hydrogel-kit","title":"3-D Life Dextran-HA Hydrogel Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe\u003cstrong\u003e \u003c\/strong\u003e\u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e Dextran-HA Hydrogel Kit\u003c\/strong\u003e provides reagents for setting up \u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels\u003c\/a\u003e containing hyaluronic acid (hyaluronan).\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIts major components are SG-Dextran and the thiol-functionalized hyaluronic acid crosslinker HyLink. When SG-Dextran and HyLink are combined, thiol groups on HyLink form stable thioether bonds with thiol-reactive groups on SG-Dextran, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrior to the crosslinking step, cell adhesion peptides (e.g. \u003ca title=\"3-D Life RGD Peptide Product Page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-rgd-peptide\" target=\"_blank\"\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e RGD Peptide, Cat. No. 09-P-001\u003c\/a\u003e) can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell-adhesive matrix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Dextran hydrogels crosslinked with HyLink cannot be dissolved by the addition of dextranase (\u003c\/span\u003e\u003ci\u003e\u003cspan id=\"Life\"\u003e3-D Life\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e Dextranase, Cat. No. D10-1).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor more information and instructions, please consult the General Protocol GP-4 \"Preparation of \u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e Hyaluronic Acid (HA) Hydrogels\" and the \u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e3-D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eAllows formation of 3.3 ml \u003c\/span\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003cspan\u003e Hydrogel of a crosslinking strength of 1.2 mmol\/L.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSG-Dextran\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e30 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life SG-Dextran\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_SG-Dextran.pdf?v=1635520621\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eHyLink, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e2 x 200 µl*\u003c\/td\u003e\n\u003ctd\u003e10 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life HyLink\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_hy-link.pdf?v=1635524487\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10x CB Buffer (pH 7.2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CB Buffer pH 7.2\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB7.2.pdf?v=1635453735\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e2 x 1500 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e Resources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"Product Datasheet (PDS): 3-D Life Dextran-HA Hydrogel SG Kit\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G95-1_2024.09.pdf?v=1727125151\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life Dextran-HA Hydrogel SG Kit\u003c\/a\u003e\u003ca title=\"Product Datasheet: 3-D Life Dextran-HA Hydrogel SG Kit\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G95-1.pdf?v=1635524628\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"3-D Life Dextran-HA Hydrogel Kit - Cat. No. G95-1","offer_id":44075914035412,"sku":"G95-1","price":336.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/g95_1.png?v=1727124854"},{"product_id":"3-d-life-pva-ha-hydrogel-kit","title":"3-D Life PVA-HA Hydrogel Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe \u003cstrong\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e PVA-HA Hydrogel Kit\u003c\/strong\u003e provides reagents for setting up \u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e H\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003eydrogels\u003c\/a\u003e containing hyaluronic acid (hyaluronan).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIts major components are SG-PVA and the thiol-functionalized hyaluronic acid crosslinker HyLink. When the two reagents are combined, thiol groups on HyLink form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrior to the crosslinking step, cell adhesion peptides (e.g. \u003ca href=\"https:\/\/ilexlife.com\/products\/3-d-life-rgd-peptide\" title=\"3-D Life RGD Peptide Product Page\" target=\"_blank\"\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e RGD Peptide, Cat. No. 09-P-001\u003c\/a\u003e) can be covalently attached to a portion of the SH-reactive groups on SG-PVA to provide a cell-adhesive matrix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor more information and instructions, please consult the General Protocol GP-4 \"Preparation of \u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e Hyaluronic Acid (HA) Hydrogels\" and the \u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e3D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels.\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003eAllows formation of 3.3 ml \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel of a crosslinking strength of 1.2 mmol\/L.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSG-PVA\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e30 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_SG-PVA.pdf?v=1635518272\" title=\"Material Safety Data Sheet (MSDS) 3-D Life SG-PVA\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eHyLink, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e2 x 200 µl*\u003c\/td\u003e\n\u003ctd\u003e10 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_hy-link.pdf?v=1635524487\" title=\"Material Safety Data Sheet (MSDS) 3-D Life HyLink\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10x CB Buffer (pH 7.2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB7.2.pdf?v=1635453735\" title=\"Material Safety Data Sheet (MSDS) 3-D Life CB Buffer pH 7.2\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e2 x 1500 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e Resources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G85-1.pdf?v=1635528795\" title=\"Product Datasheet: 3-D Life PVA-HA Hydrogel Kit\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life PVA-HA Hydrogel Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" title=\"3-D Life Biomimetic Hydrogels Page\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"3-D Life PVA-HA Hydrogel Kit - Cat. No. G85-1","offer_id":44075967545556,"sku":"G85-1","price":333.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/g85_1.png?v=1729793234"},{"product_id":"3-d-life-dextran-peg-hydrogel-fg-kit","title":"3-D Life Dextran-PEG Hydrogel FG Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Dextran-PEG Hydrogel FG Kit\u003c\/strong\u003e provides reagents for setting up fast gelling, cell-compatible \u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cem\u003e3-D Life \u003c\/em\u003eHydrogels\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003eIts major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrior to the crosslinking step, cell adhesion peptides (e.g. \u003ca title=\"3-D Life RGD Peptide Product Page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-rgd-peptide\" target=\"_blank\"\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e RGD Peptide, Cat. No. 09-P-001\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eDextran hydrogels crosslinked with PEG-Link can be dissolved by the addition of dextranase (\u003ca title=\"3-D Life Dextranase Product Page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-dextranase\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Dextranase, Cat. No. D10-1\u003c\/a\u003e), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor instructions, please consult the General Protocol GP-1 \"Preparation of \u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e Fast Gelling Hydrogels\" and the \u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e3D cell cultures requiring no cell spreading and migration; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003eAllows formation of up to 2 ml \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel depending on the grade of stiffness of the gel.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMal-Dextran, lyophilized\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e30 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Mal-Dextran\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_Mal-Dextran.pdf?v=1635529469\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003ePEG-Link, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl*\u003c\/td\u003e\n\u003ctd\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life PEG-Link\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_PEG-Link.pdf?v=1635518317\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10x CB Buffer (pH 5.5)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CB Buffer pH 5.5\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB5.5.pdf?v=1635452874\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e2 x 1500 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"Product Datasheet: 3-D Life Dextran-PEG Hydrogel FG Kit\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_FG90-1_Ilex.pdf?v=1729791923\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life Dextran-PEG Hydrogel FG Kit\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_FG90-1.pdf?v=1635529638\" title=\"Product Datasheet: 3-D Life Dextran-PEG Hydrogel FG Kit\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" title=\"3-D Life Biomimetic Hydrogels Page\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca title=\"Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Application_Note_1_-_Epithelial_Cyst_Formation_in_3-D_Life_Hydrogels.pdf?v=1635455275\" target=\"_blank\"\u003e\u003cstrong\u003eApplication Note:\u003c\/strong\u003e Epithelial Cyst Formation in 3-D Life Hydrogels\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/p_1_cyst_formation_-_MDCK_cyst_formation_in_3-D_Life_Hydrogels.pdf?v=1635455325\" target=\"_blank\"\u003e\u003cstrong\u003eApplication Protocol:\u003c\/strong\u003e MDCK Cyst Formation in 3-D Life Hydrogels\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGuenther, Carla. “Stiffness regulates dendritic cell and macrophage subtype development and increased stiffness induces a tumor-associated macrophage phenotype in cancer co-cultures.” \u003ci\u003eFrontiers in immunology\u003c\/i\u003e vol. 15 1434030. 15 Aug. 2024, \u003ca rel=\"noopener\" title=\"Guenther, Carla. “Stiffness regulates dendritic cell and macrophage subtype development and increased stiffness induces a tumor-associated macrophage phenotype in cancer co-cultures.” Frontiers in immunology vol. 15 1434030. 15 Aug. 2024, doi:10.3389\/fimmu.2024.1434030\" href=\"https:\/\/doi.org\/10.3389\/fimmu.2024.1434030\" target=\"_blank\"\u003edoi:10.3389\/fimmu.2024.1434030\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eMohammadi Ayenehdeh, Jamal et al. “Introducing a New Experimental Islet Transplantation Model using Biomimetic Hydrogel and a Simple High Yield Islet Isolation Technique.” \u003ci\u003eIranian biomedical journal\u003c\/i\u003e vol. 21,4 (2017): 218-27. \u003ca rel=\"noopener\" title=\"Mohammadi Ayenehdeh, Jamal et al. “Introducing a New Experimental Islet Transplantation Model using Biomimetic Hydrogel and a Simple High Yield Islet Isolation Technique.” Iranian biomedical journal vol. 21,4 (2017): 218-27. doi:10.18869\/acadpub.ibj.21.4.218\" href=\"https:\/\/doi.org\/10.18869\/acadpub.ibj.21.4.218\" target=\"_blank\"\u003edoi:10.18869\/acadpub.ibj.21.4.218\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"3-D Life Dextran-PEG Hydrogel FG Kit - Cat. No. FG90-1","offer_id":44075932352724,"sku":"FG90-1","price":153.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/fg90_1_1913003921.png?v=1729790899"},{"product_id":"3-d-life-dextran-cd-hydrogel-fg-kit","title":"3-D Life Dextran-CD Hydrogel FG Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe \u003c\/span\u003e\u003cstrong\u003e\u003cem\u003e3-D Life\u003c\/em\u003e\u003c\/strong\u003e\u003cspan\u003e\u003cstrong\u003e Dextran-CD Hydrogel FG Kit\u003c\/strong\u003e provides reagents for setting up fast gelling, cell-compatible \u003ca href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" title=\"3-D Life Biomimetic Hydrogels\" rel=\"noopener\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIts major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9. It allows cells to spread and migrate within the hydrogel if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrior to the crosslinking step, cell adhesion peptides (e.g. \u003ca title=\"3-D Life RGD Peptide Product Page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-rgd-peptide\" target=\"_blank\"\u003e\u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e RGD Peptide, Cat. No. 09-P-001\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eDextran hydrogels crosslinked with CD-Link can be dissolved by the addition of dextranase (\u003ca title=\"3-D Life Dextranase Product Page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-dextranase\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Dextranase, Cat. No. D10-1\u003c\/a\u003e), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor instructions, please consult the General Protocol GP-1 \"Preparation of \u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e Fast Gelling Hydrogels\" and the \u003cem data-mce-fragment=\"1\"\u003e3-D Life\u003c\/em\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e3D cell cultures\u003c\/p\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003eAllows formation of up to 2 ml \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel depending on the grade of stiffness of the gel.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMal-Dextran, lyophilized\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e30 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Mal-Dextran\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_Mal-Dextran.pdf?v=1635529469\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eCD-Link, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl*\u003c\/td\u003e\n\u003ctd\u003e20 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CD-Link\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CD-Link.pdf?v=1635452149\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10x CB Buffer (pH 5.5)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CB Buffer pH 5.5\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB5.5.pdf?v=1635452874\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e2 x 1500 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_FG91-1_2024_Ilex.pdf?v=1729704695\" title=\"Product Datasheet: 3-D Life Dextran-CD Hydrogel FG Kit\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life Dextran-CD Hydrogel FG Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" title=\"3-D Life Biomimetic Hydrogels Page\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/p_3_co-culture_tumor_and_stroma_cells.pdf?v=1635455197\" title=\"Application Protocol: Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels\" target=\"_blank\"\u003e\u003cstrong\u003eApplication Protocol:\u003c\/strong\u003e Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/app_note_3_-_Co-culture_of_tumor_and_stroma_cells_in_3-D_Life_Hydrogels.pdf?v=1635455437\" title=\"Application Note: Co-culture of tumor and stroma cells in 3-D Life Hydrogels\" target=\"_blank\"\u003e\u003cstrong\u003eApplication Note:\u003c\/strong\u003e Co-culture of tumor and stroma cells in 3-D Life Hydrogels\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct References\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGuenther, Carla. “Stiffness regulates dendritic cell and macrophage subtype development and increased stiffness induces a tumor-associated macrophage phenotype in cancer co-cultures.” \u003ci\u003eFrontiers in immunology\u003c\/i\u003e vol. 15 1434030. 15 Aug. 2024,\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3389\/fimmu.2024.1434030\" title=\"Guenther, Carla. “Stiffness regulates dendritic cell and macrophage subtype development and increased stiffness induces a tumor-associated macrophage phenotype in cancer co-cultures.” Frontiers in immunology vol. 15 1434030. 15 Aug. 2024, doi:10.3389\/fimmu.2024.1434030\" rel=\"noopener\" aria-describedby=\"a11y-new-window-external-message\" target=\"_blank\"\u003edoi:10.3389\/fimmu.2024.1434030\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eWang, Zheng et al. “Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.” \u003ci\u003eMaterials (Basel, Switzerland)\u003c\/i\u003e vol. 12,20 3391. 17 Oct. 2019, \u003ca rel=\"noopener\" title=\"Wang, Zheng et al. “Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.” Materials (Basel, Switzerland) vol. 12,20 3391. 17 Oct. 2019, doi:10.3390\/ma12203391\" href=\"https:\/\/doi.org\/10.3390\/ma12203391\" target=\"_blank\"\u003edoi:10.3390\/ma12203391\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"3-D Life Dextran-CD Hydrogel FG Kit - Cat. No. FG91-1","offer_id":44075897979092,"sku":"FG91-1","price":331.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/fg91_1_75182228.png?v=1729704226"},{"product_id":"3-d-life-dextran-cd-ha-hydrogel-kit","title":"3-D Life Dextran-CD-HA Hydrogel Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe \u003cstrong\u003e\u003ci\u003e\u003cspan id=\"Life\"\u003e3-D Life\u003c\/span\u003e\u003c\/i\u003e Dextran-CD-HA Hydrogel Kit\u003c\/strong\u003e provides reagents for setting up cell-degradable \u003ca rel=\"noopener\" title=\"3-D Life Biomimetic Hydrogels\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e Hydrogels\u003c\/a\u003e containing hyaluronic acid (hyaluronan).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIts major components are SG-Dextran and the thiol-functionalized hyaluronic acid crosslinker CD-HyLink. When SG-Dextran and CD-HyLink are combined, thiol groups on CD-HyLink form stable thioether bonds with thiol-reactive groups on SG-Dextran, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCD-HyLink contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrior to the crosslinking step, cell adhesion peptides (e.g. \u003ca title=\"3-D Life RGD Peptide Product Page\" href=\"https:\/\/ilexlife.com\/products\/3-d-life-rgd-peptide\" target=\"_blank\"\u003e\u003cem\u003e3-D Life\u003c\/em\u003e RGD Peptide, Cat. No. 09-P-001\u003c\/a\u003e) can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell-adhesive matrix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Dextran hydrogels crosslinked with CD-HyLink cannot be dissolved by the addition of dextranase (\u003c\/span\u003e\u003ci\u003e\u003cspan id=\"Life\"\u003e3-D Life\u003c\/span\u003e\u003c\/i\u003e\u003cspan\u003e Dextranase, Cat. No. D10-1).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFor more information and instructions, please consult the General Protocol GP-4B \"Preparation of \u003ci\u003e\u003cspan id=\"Life\"\u003e3-D Life\u003c\/span\u003e\u003c\/i\u003e Hyaluronic Acid (HA) Hydrogels containing a matrix metalloproteinase cleavage site\" and the \u003ci\u003e\u003cspan id=\"Life\"\u003e3-D Life\u003c\/span\u003e\u003c\/i\u003e Hydrogels User Guide, which may be downloaded below.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e3-D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable id=\"Components\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eConcentration of reactive groups\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSG-Dextran\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e30 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life SG-Dextran\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_SG-Dextran.pdf?v=1635520621\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eCD-HyLink, lyophilized\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e2 x 200 µl*\u003c\/td\u003e\n\u003ctd\u003e10 mmol\/L*\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/SDS_CD-HyLink_freeze-dried-4.pdf?v=1760110919\" title=\"Material Safety Data Sheet (MSDS): 3-D Life CD-HyLink Hydrogel Component\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003e10x CB Buffer (pH 7.2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e200 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life CB Buffer pH 7.2\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_CB7.2.pdf?v=1635453735\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater\u003c\/td\u003e\n\u003ctd\u003e2 x 1500 µl\u003c\/td\u003e\n\u003ctd\u003en\/a\u003c\/td\u003e\n\u003ctd\u003e\u003ca title=\"Material Safety Data Sheet (MSDS) 3-D Life Water\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/msds_water.pdf?v=1635452280\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e*Volume\/concentration after reconstitution of lyophilizate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eQuantity\u003c\/h2\u003e\n\u003cp\u003e Allows formation of 3.3 ml \u003cem\u003e3-D Life\u003c\/em\u003e Hydrogel of a crosslinking strength of 1.2 mmol\/L.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G96-1_2025.pdf?v=1760038640\" title=\"Product Datasheet (PDS): 3-D Life Dextran-CD-HA Hydrogel Kit\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eProduct Datasheet (PDS):\u003c\/strong\u003e 3-D Life Dextran-CD-HA Hydrogel Kit\u003c\/a\u003e\u003ca title=\"Product Datasheet: 3-D Life Dextran-HA Hydrogel SG Kit\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/pds_G95-1.pdf?v=1635524628\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-guide-protocols\" title=\"3-D Life Hydrogels User Guide \u0026amp; Protocols\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e3-D Life User Guide \u0026amp; Protocols\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca title=\"3-D Life Biomimetic Hydrogels Page\" href=\"https:\/\/ilexlife.com\/pages\/biomimetic-hydrogels-for-3d-cell-culture\" target=\"_blank\"\u003e3-D Life Biomimetic Hydrogels Page\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-user-applications\" title=\"3-D Life Hydrogels User Applications: referenced in scientific literature for use in a wide variety of applications with many different cell lines and primary cells\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels User Applications\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/pages\/3-d-life-hydrogels-references\" title=\"3-D Life Hydrogels Product Refences: citations in published papers for use in a wide variety of 3D cell culture applications\" target=\"_blank\"\u003e3-D Life\u003cspan\u003e \u003c\/span\u003eHydrogels Product References\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized distributor of Cellendes GmbH.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"3-D Life Dextran-CD-HA Hydrogel Kit - Cat. No. G96-1","offer_id":47034336018644,"sku":"G96-1","price":610.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/g96_1_png.png?v=1761586378"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/collections\/ready-to-design.jpg?v=1635354733","url":"https:\/\/ilexlife.com\/collections\/ready-to-design-hydrogel-kits\/3-d-life-biomimetic-hydrogels.oembed","provider":"Ilex Life Sciences","version":"1.0","type":"link"}