96 well plate for ANGPTL3 ELISA

ANGPTL3 (Angiopoietin-like protein 3) Human ELISA

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Principle of Method

This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human ANGPTL3 in biological fluids. A monoclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a purified polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples.

Description

The angiopoietins are a family of growth factors that are specific for vascular endothelium. Conklin et al. (1) isolated a full-length cDNA encoding angiopoietin-like protein 3 (ANGPTL3) from a human fetal liver/spleen cDNA library. The deduced 460-amino acid ANGPTL3 protein has the characteristic structure of angiopoietins: a signal peptide, an extended helical domain predicted to form dimeric or trimeric coiled-coils, a short linker peptide, and a globular fibrinogen-like domain (FLD). Human ANGPTL3 shares 76% amino acid sequence identity with mouse Angptl3.  Northern blot analysis of human tissues showed a preferential expression of 4 ANGPTL3 transcripts being 4.5, 3.0, 2.8, and 1.7 kb in liver.

Camenisch et al. (2) showed that ANGPTL3 induced angiogenesis in a rat corneal assay. ANGPTL3 is structurally similar to angiopoietins, which are vascular endothelial growth factors. The experimental results show that Angptl3 and Angptl4 function to regulate circulating triglyceride levels during different nutritional states and therefore play a role in lipid metabolism during feeding/fasting through differential inhibition of Lipoprotein lipase (LPL).  Overexpression of Angptl3 or intravenous injection of the purified protein in KK/San mice elicited an increase in circulating plasma lipid levels. These data suggested that Angptl3 regulates lipid metabolism in animals. The authors suggested the possibility that genetic variation in ANGPTL3 contributes to atherosclerosis, coronary artery disease, and diabetes mellitus. In vitro analysis of recombinant protein revealed that Angptl3 directly inhibits both endothelial lipase and lipoprotein lipase (LPL) activity (5, 6). Another line of evidence suggests that ANGPTL3 play an important role in regulation of HDL synthesis (7). The implication of ANGPTL3 in a number of metabolic dysfunctions suggests that ANGPTL3 is a novel predictor of these.  Given these findings, ANGPTL3 has potential as a novel therapeutic target for treatment of hyperlipidemia.

Type

Sandwich type ELISA

Other names

Angiopoietin-like protein 3, 

Sample Types

Serum, Plasma or Cell Culture Supernatant

Species crossreactivity

Human ANGPTL3.  No other known species crossreactivity

Sample Volume

Serum and Plasma : 50 µL (actual amount of sample loaded in well after dilution)

Standard Curve

Standard curve with the Human ANGPTL3 ELISA kit

Calibration Range: 0.156 ng/ml – 10 ng/ml

Limit of Detection

75 pg/ml

Intra-assay (Within-Run) C.V.

1.64%

Inter-assay (Run-to-Run) C.V.

6.88%

Resources

References to Summary

  • Identification of a mammalian angiopoietin-related protein expressed specifically in liver: D. Conklin, et al.; Genomics 62, 477 (1999)
  • ANGPTL3 stimulates endothelial cell adhesion and migration via integrin alpha-v-beta-3 and induces blood vessel formation in vivo: G. Camenisch, et al.; J. Biol. 277, 17281 (2002)
  • Angiopoietin-like proteins stimulate ex vivo expansion of hematopoietic stem cells: C.C. Zhang, et al.; Nature Med. 12, 240 (2006)
  • Angptl3 regulates lipid metabolism in mice: R. Koishi, et al.; Nature Genet. 30, 151 (2002)
  • ANGPTL3 decreases very low density lipoprotein triglyceride clearance by inhibition of lipoprotein lipase: T. Shimizugawa, et al.; J. Biol. 277, 33742 (2002)
  • Angiopoietin-like protein3 regulates plasma HDL cholesterol through suppression of endothelial lipase: M. Shimamura, et al.; Arterioscler. Vasc. Biol. 27, 366 (2007)
  • Hepatic proprotein convertases modulate HDL metabolism: W. Jin, et al.; Cell Metab. 6, 129 (2007)