{"product_id":"isokine-il-15-human-animal-free-recombinant-protein","title":"ISOkine® IL-15 Human, Animal-Free Recombinant Protein","description":"\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"ISOkine Premium Growth Factors \u0026amp; Cytokines banner\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/ISOkine_banner.png?v=1776108792\"\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eThe cytokine that preserves the stem-cell-memory phenotype.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003e\u003cstrong\u003eISOkine® Interleukin-15 (IL-15) Human, Animal-Free Recombinant Protein\u003c\/strong\u003e \u003c\/em\u003eis a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains \u003c\/span\u003e\u003cspan\u003e114 amino acids and an N-terminal 17 amino acid histidine tag for a total length of 131 amino acids and has a predicted molecular mass of 15.04 kDa.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eISOkine® growth factors and cytokines are animal-free (AOF\/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eCAR-T \u0026amp; TIL Expansion:\u003c\/strong\u003e Preserves TSCM phenotype during long culture.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eNK Cell Proliferation \u0026amp; Survival:\u003c\/strong\u003e Supports expansion and cytotoxic function.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eMemory-like NK Induction:\u003c\/strong\u003e Essential in IL-12\/IL-15\/IL-18 protocols.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eiPSC-derived Immune Cells:\u003c\/strong\u003e Enables efficient expansion of NK and CAR-NK cells.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eNKT \u0026amp; CAR-NKT Differentiation:\u003c\/strong\u003e From hematopoietic progenitor cells.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eDefined Media Formulation:\u003c\/strong\u003e Ideal for serum-free, chemically-defined systems.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eSummary\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eInterleukin-15 (IL-15) is a cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems. Stimulates the proliferation of natural killer cells, T-cells, and B-cells and promotes the secretion of several cytokines. In monocytes, induces the production of IL8 and monocyte chemotactic protein 1\/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection. Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits.\u003c\/p\u003e\n\u003ch2 class=\"Heading_h5__GFC_g TextAndLink_textAndLink__title__H9HnQ\"\u003eSource\u003c\/h2\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003e\u003cstrong\u003eExpression Host: \u003c\/strong\u003eProduced in the endosperm tissue of barley grain \u003cem\u003e(Hordeum vulgare) \u003c\/em\u003eplants.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eManufacturer: \u003c\/strong\u003eORF Genetics\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCountry of Origin: \u003c\/strong\u003eIceland\u003cbr\u003e\u003c\/p\u003e\n\u003ch2 class=\"Heading_h5__GFC_g TextAndLink_textAndLink__title__H9HnQ\"\u003eFormulation\u003c\/h2\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003e2xPBS pH 7.2, sterile filtered through 0.22 µm filter\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003ePurity\u003c\/h2\u003e\n\u003cdiv class=\"TextAndLink_textAndLink__1sUC6\"\u003e\n\u003cdiv class=\"TextAndLink_textAndLink__content__4hGPr\"\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003e≥ 90% by SDS-PAGE gel analysis.\u003c\/p\u003e\n\u003ch2 class=\"Heading_h5__GFC_g TextAndLink_textAndLink__title__H9HnQ\"\u003eReconstitution\u003c\/h2\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003eAlways centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg\/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% \u003ca rel=\"noopener\" title=\"HSA \/ BSA Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" target=\"_blank\"\u003eHSA or BSA\u003c\/a\u003e).\u003c\/p\u003e\n\u003ch2\u003eStability\u003c\/h2\u003e\n\u003cp\u003eThe lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. \u003cstrong\u003eAvoid repeated freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 class=\"TextAndLink_textAndLink__content__4hGPr\"\u003eBiological Activity\u003c\/h2\u003e\n\u003cp style=\"text-align: start;\" class=\"TextAndLink_textAndLink__content__4hGPr\"\u003eEach batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant human IL-15 is assayed by measuring its dose-dependent effect on the proliferation of CTLL-2 cells. The ED50 for this effect using IL-15 is typically ≤2 ng\/ml. Optimal concentration should be determined for specific applications and cell lines.\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/ISOkine_IL-15_bioactivity.png?v=1791225052\" alt=\"Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a\u0026amp;nbsp;commercial source. The bioactivity of ISOkine® recombinant human IL-15 is assayed by measuring its dose-dependent effect on the proliferation of CTLL-2 cells. The ED50 for this effect using IL-15 is typically ≤2 ng\/ml.\u0026amp;nbsp;Optimal concentration should be determined for specific applications and cell lines.\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"TextAndLink_textAndLink__1sUC6\"\u003e\n\u003cdiv class=\"TextAndLink_textAndLink__content__4hGPr\"\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003ch2 class=\"Heading_h5__GFC_g TextAndLink_textAndLink__title__H9HnQ\"\u003eEndotoxin Level\u003c\/h2\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003eEndotoxin level is \u0026lt; 0.01 EU per μg of ISOkine® product by LAL method.\u003c\/p\u003e\n\u003ch2\u003ePyrogen Free\u003c\/h2\u003e\n\u003cp class=\"RichText_richText___0Wgg\"\u003ePurified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003elaboratory research use only\u003c\/strong\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"product-specs-content\"\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eSynonyms\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eInterleukin 15, IL15, UniProtKB# P40933.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/orfgenetics-documents-ik2900il-15humandsnew.pdf?v=1791224841\" title=\"Product Datasheet (PDS): ISOkine® IL-15 Human, Animal-Free Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/orfgenetics-documents-ik2900il-15humansds.pdf?v=1791224842\" title=\"Material Safety Data Sheet (MSDS): ISOkine® IL-15 Human, Animal-Free Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eMaterial Safety Data Sheet (MSDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/orfgenetics-documents-ik2900il-15humancoo.pdf?v=1791224841\" title=\"Certificate of Origin (CoO): ISOkine® IL-15 Human, Animal-Free Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eCertificate of Origin (CoO)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"ISOkine® Premium Animal-Free Growth Factors \u0026amp; Cytokines\" href=\"https:\/\/ilexlife.com\/collections\/isokine-premium-animal-free-growth-factors-cytokines\" target=\"_blank\"\u003e\u003cspan\u003eISOkine® Premium Animal-Free Growth Factors \u0026amp; Cytokines\u003c\/span\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/collections\/cytokines\" title=\"Cytokines \u0026amp; Growth Factors Product Collection\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003eCytokines \u0026amp; Growth Factors\u003c\/span\u003e\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"KGF (FGF-7) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/kgf-fgf-7-human-e-coli-recombinant-protein\" target=\"_blank\"\u003e\u003c\/a\u003e\u003cem\u003e\u003c\/em\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/isokine-il-7-human-animal-free-recombinant-protein\" title=\"ISOkine® IL-7 Human, Animal-Free Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eISOkine® IL-7 Human, Animal-Free Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/products\/isokine-il-2-human-animal-free-recombinant-protein\" title=\"ISOkine® IL-2 Human, Animal-Free Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eISOkine® IL-2 Human, Animal-Free Recombinant Protein\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/interleukin-15-il-15-human-e-coli-recombinant-protein\" title=\"Interleukin-15 (IL-15) Human, E. coli Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003eIL-15 Human, E. coli Recombinant Protein\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/interleukin-15-il-15-rat-e-coli-recombinant-protein\" title=\"Interleukin-15 (IL-15) Rat, E. coli Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003eIL-15 Rat, E. coli Recombinant Protein\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors \u0026amp; cytokines manufactured by ORF Genetics Ltd.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"10 µg - Cat. No. IK2900-0010","offer_id":67641066553556,"sku":"IK2900-0010","price":170.0,"currency_code":"USD","in_stock":true},{"title":"50 µg - Cat. No. IK2900-0050","offer_id":67641066586324,"sku":"IK2900-0050","price":444.0,"currency_code":"USD","in_stock":true},{"title":"100 µg - Cat. No. IK2900-0100","offer_id":67641066619092,"sku":"IK2900-0100","price":684.0,"currency_code":"USD","in_stock":true},{"title":"1000 µg - Cat. No. IK2900-1000","offer_id":67641066651860,"sku":"IK2900-1000","price":3414.0,"currency_code":"USD","in_stock":true},{"title":"2000 µg - Cat. No. IK2900-2000","offer_id":67641066684628,"sku":"IK2900-2000","price":5460.0,"currency_code":"USD","in_stock":true},{"title":"5000 µg - Cat. No. IK2900-5000","offer_id":67641066717396,"sku":"IK2900-5000","price":12272.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/IL-15_ISOkine.jpg?v=1791914333","url":"https:\/\/ilexlife.com\/products\/isokine-il-15-human-animal-free-recombinant-protein","provider":"Ilex Life Sciences","version":"1.0","type":"link"}