{"product_id":"isokine-il-7-human-animal-free-recombinant-protein","title":"ISOkine® IL-7 Human, Animal-Free Recombinant Protein","description":"\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"ISOkine Premium Growth Factors \u0026amp; Cytokines banner\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/ISOkine_banner.png?v=1776108792\"\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eThe survival cytokine in every memory-enriched T-cell expansion protocol.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003e\u003cstrong\u003eISOkine® Interleukin-7 (IL-7) Human, Animal-Free Recombinant Protein\u003c\/strong\u003e \u003c\/em\u003eis a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains \u003c\/span\u003e\u003cspan\u003e152 amino acids and an N-terminal 17 amino acid histidine tag for a total length of 169 amino acids and has a predicted molecular mass of 19.7 kDa.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eISOkine® growth factors and cytokines are animal-free (AOF\/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eLymphocyte Development \u0026amp; Expansion:\u003c\/strong\u003e Supports T-cell and B-cell proliferation and survival.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eImmune Response \u0026amp; Immunotherapy Research:\u003c\/strong\u003e Enhances immune function and anti-tumor activity.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eStem Cell Research \u0026amp; Regenerative Medicine:\u003c\/strong\u003e Promotes stem cell maintenance and differentiation.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eTissue Repair \u0026amp; Wound Healing:\u003c\/strong\u003e Supports tissue regeneration and recovery.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eDefined Media Formulation:\u003c\/strong\u003e Ideal for serum-free, chemically-defined systems.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eSummary\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eInterleukin-7 (IL-7) is a hematopoietic cytokine that plays an essential role in the development, expansion, and survival of T-cells (naive and memory) and B-cells. Thereby helps with regulating the number of mature lymphocytes and maintaining lymphoid homeostasis by interaction with receptors composed of IL7RA and gamma\/CSF2RG and activating various kinases (incl. JAK1 and JAK3).\u003cbr\u003e\u003c\/p\u003e\n\u003ch2 class=\"Heading_h5__GFC_g TextAndLink_textAndLink__title__H9HnQ\"\u003eSource\u003c\/h2\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003e\u003cstrong\u003eExpression Host: \u003c\/strong\u003eProduced in the endosperm tissue of barley grain \u003cem\u003e(Hordeum vulgare) \u003c\/em\u003eplants.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eManufacturer: \u003c\/strong\u003eORF Genetics\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCountry of Origin: \u003c\/strong\u003eIceland\u003cbr\u003e\u003c\/p\u003e\n\u003ch2 class=\"Heading_h5__GFC_g TextAndLink_textAndLink__title__H9HnQ\"\u003eFormulation\u003c\/h2\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003e2xPBS pH 7.2, sterile filtered through 0.22 µm filter\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003ePurity\u003c\/h2\u003e\n\u003cdiv class=\"TextAndLink_textAndLink__1sUC6\"\u003e\n\u003cdiv class=\"TextAndLink_textAndLink__content__4hGPr\"\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003e≥ 95% by SDS-PAGE gel analysis.\u003c\/p\u003e\n\u003ch2 class=\"Heading_h5__GFC_g TextAndLink_textAndLink__title__H9HnQ\"\u003eReconstitution\u003c\/h2\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003eAlways centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg\/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% \u003ca rel=\"noopener\" title=\"HSA \/ BSA Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" target=\"_blank\"\u003eHSA or BSA\u003c\/a\u003e).\u003c\/p\u003e\n\u003ch2\u003eStability\u003c\/h2\u003e\n\u003cp\u003eThe lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. \u003cstrong\u003eAvoid repeated freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 class=\"TextAndLink_textAndLink__content__4hGPr\"\u003eBiological Activity\u003c\/h2\u003e\n\u003cp style=\"text-align: start;\" class=\"TextAndLink_textAndLink__content__4hGPr\"\u003eEach batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant human IL-7 is assayed by measuring its dose-dependent effect on the proliferation of 2E8 cells. The ED50 for this effect using IL-7 is typically ≤2 ng\/ml. Optimal concentration should be determined for specific applications and cell lines.\u003c\/p\u003e\n\u003cdiv class=\"TextAndLink_textAndLink__1sUC6\"\u003e\n\u003cdiv class=\"TextAndLink_textAndLink__content__4hGPr\"\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003ch2 class=\"Heading_h5__GFC_g TextAndLink_textAndLink__title__H9HnQ\"\u003eEndotoxin Level\u003c\/h2\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003eEndotoxin level is \u0026lt; 0.01 EU per μg of ISOkine® product by LAL method.\u003c\/p\u003e\n\u003ch2\u003ePyrogen Free\u003c\/h2\u003e\n\u003cp class=\"RichText_richText___0Wgg\"\u003ePurified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003elaboratory research use only\u003c\/strong\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"product-specs-content\"\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eSynonyms\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eInterleukin 7, IL7, UniProtKB# P13232.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/IK3200_IL-7_human_DS.pdf?v=1792082751\" title=\"Product Datasheet (PDS): ISOkine® IL-7 Human, Animal-Free Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/orfgenetics-documents-ik2900il-15humansds.pdf?v=1791224842\" title=\"Material Safety Data Sheet (MSDS): ISOkine® IL-15 Human, Animal-Free Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"ISOkine® Premium Animal-Free Growth Factors \u0026amp; Cytokines\" href=\"https:\/\/ilexlife.com\/collections\/isokine-premium-animal-free-growth-factors-cytokines\" target=\"_blank\"\u003e\u003cspan\u003eISOkine® Premium Animal-Free Growth Factors \u0026amp; Cytokines\u003c\/span\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/cytokines\" title=\"Cytokines \u0026amp; Growth Factors Product Collection\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003eCytokines \u0026amp; Growth Factors\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/isokine-il-15-human-animal-free-recombinant-protein\" title=\"ISOkine® IL-15 Human, Animal-Free Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eISOkine® IL-15 Human, Animal-Free Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/products\/isokine-il-2-human-animal-free-recombinant-protein\" title=\"ISOkine® IL-2 Human, Animal-Free Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eISOkine® IL-2 Human, Animal-Free Recombinant Protein\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors \u0026amp; cytokines manufactured by ORF Genetics Ltd.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"10 µg - Cat. No. IK3200-0010","offer_id":67749197381844,"sku":"IK3200-0010","price":170.0,"currency_code":"USD","in_stock":true},{"title":"50 µg - Cat. No. IK3200-0050","offer_id":67749197414612,"sku":"IK3200-0050","price":547.0,"currency_code":"USD","in_stock":true},{"title":"100 µg - Cat. No. IK3200-0100","offer_id":67749197447380,"sku":"IK3200-0100","price":684.0,"currency_code":"USD","in_stock":true},{"title":"1000 µg - Cat. No. IK3200-1000","offer_id":67749197480148,"sku":"IK3200-1000","price":4095.0,"currency_code":"USD","in_stock":true},{"title":"2000 µg - Cat. No. IK3200-2000","offer_id":67749197512916,"sku":"IK3200-2000","price":6484.0,"currency_code":"USD","in_stock":true},{"title":"5000 µg - Cat. No. IK3200-5000","offer_id":67749197545684,"sku":"IK3200-5000","price":12272.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/IL-7_banner.png?v=1792083118","url":"https:\/\/ilexlife.com\/products\/isokine-il-7-human-animal-free-recombinant-protein","provider":"Ilex Life Sciences","version":"1.0","type":"link"}