{"product_id":"isokine-scf-mouse-animal-free-recombinant-protein","title":"ISOkine® SCF Mouse, Animal-Free Recombinant Protein","description":"\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"ISOkine Premium Growth Factors \u0026amp; Cytokines banner\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/ISOkine_banner.png?v=1776108792\"\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003e\u003cstrong\u003eISOkine® SCF Mouse, Animal-Free Recombinant Protein\u003c\/strong\u003e \u003c\/em\u003eis a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 166 amino acids and an N-terminal 16 amino acid histidine tag for a total length of 182 amino acids and has a predicted molecular mass of 20.6 kDa.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eISOkine® growth factors and cytokines are animal-free (AOF\/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eSummary\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStem Cell Factor (SCF) is a hematopoietic growth factor acting at the early stages of hematopoiesis. SCF promotes the survival, proliferation, and differentiation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also stimulates the growth and activation of mast cells and eosinophils. SCF has been shown to act synergistically with other growth factors, such as GM-CSF, G-CSF, IL-7 and EPO. Human SCF shows low activity on murine cells, while murine and rat SCF are fully active on human cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 class=\"Heading_h5__GFC_g TextAndLink_textAndLink__title__H9HnQ\"\u003eSource\u003c\/h2\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003e\u003cstrong\u003eExpression Host: \u003c\/strong\u003eProduced in the endosperm tissue of barley grain \u003cem\u003e(Hordeum vulgare) \u003c\/em\u003eplants.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eManufacturer: \u003c\/strong\u003eORF Genetics\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCountry of Origin: \u003c\/strong\u003eIceland\u003cbr\u003e\u003c\/p\u003e\n\u003ch2 class=\"Heading_h5__GFC_g TextAndLink_textAndLink__title__H9HnQ\"\u003eFormulation\u003c\/h2\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003e2xPBS pH 7.2, sterile filtered through 0.22 µm filter\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003ePurity\u003c\/h2\u003e\n\u003cdiv class=\"TextAndLink_textAndLink__1sUC6\"\u003e\n\u003cdiv class=\"TextAndLink_textAndLink__content__4hGPr\"\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003e\u0026gt; 95% by SDS-PAGE gel analysis.\u003c\/p\u003e\n\u003ch2 class=\"Heading_h5__GFC_g TextAndLink_textAndLink__title__H9HnQ\"\u003eReconstitution\u003c\/h2\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003eAlways centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg\/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% \u003ca rel=\"noopener\" title=\"HSA \/ BSA Product Collection\" href=\"https:\/\/ilexlife.com\/collections\/bsa-hsa-serum-albumin\" target=\"_blank\"\u003eHSA or BSA\u003c\/a\u003e).\u003c\/p\u003e\n\u003ch2\u003eStability\u003c\/h2\u003e\n\u003cp\u003eThe lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. \u003cstrong\u003eAvoid repeated freeze-thaw cycles.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 class=\"TextAndLink_textAndLink__content__4hGPr\"\u003eBiological Activity\u003c\/h2\u003e\n\u003cp style=\"text-align: start;\" class=\"TextAndLink_textAndLink__content__4hGPr\"\u003eEach batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant mouse SCF is assayed by measuring its dose-dependent effect on the proliferation of TF-1 cells. The ED50 for this effect using SCF is typically \u0026lt;20 ng\/ml. Optimal concentration should be determined for specific applications and cell lines.\u003c\/p\u003e\n\u003cdiv class=\"TextAndLink_textAndLink__1sUC6\"\u003e\n\u003cdiv class=\"TextAndLink_textAndLink__content__4hGPr\"\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003ch2 class=\"Heading_h5__GFC_g TextAndLink_textAndLink__title__H9HnQ\"\u003eEndotoxin Level\u003c\/h2\u003e\n\u003cdiv class=\"RichText_richText___0Wgg\"\u003e\n\u003cp\u003eEndotoxin level is \u0026lt; 0.01 EU per μg of ISOkine® product by LAL method.\u003c\/p\u003e\n\u003ch2\u003ePyrogen Free\u003c\/h2\u003e\n\u003cp class=\"RichText_richText___0Wgg\"\u003ePurified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003eUsage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003elaboratory research use only\u003c\/strong\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"product-specs-content\"\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eSynonyms\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eMurine SCF, KIT ligand, c-Kit ligand, KITLG, KL, KL-1, KITL, mast cell growth factor, MGF, Steel factor, SF.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"Product Datasheet (PDS): ISOkine® SCF Mouse, Animal-Free Recombinant Protein\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/aYChFN0YXLCxVQ3O_IK2501SCFmouseDS.pdf?v=1777490132\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/aYNX3d0YXLCxVXXn_IK2501SCFmouseCoO.pdf?v=1777490132\" title=\"Certificate of Origin (CoO): ISOkine® SCF Mouse, Animal-Free Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eCertificate of Origin (CoO)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"product-specs-content\"\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"ISOkine® Premium Animal-Free Growth Factors \u0026amp; Cytokines\" href=\"https:\/\/ilexlife.com\/collections\/isokine-premium-animal-free-growth-factors-cytokines\" aria-describedby=\"a11y-new-window-message\" target=\"_blank\"\u003e\u003cspan\u003eISOkine® Premium Animal-Free Growth Factors \u0026amp; Cytokines\u003c\/span\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/collections\/cytokines\" title=\"Cytokines \u0026amp; Growth Factors Product Collection\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003eCytokines \u0026amp; Growth Factors\u003c\/span\u003e\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"KGF (FGF-7) Human, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/kgf-fgf-7-human-e-coli-recombinant-protein\" target=\"_blank\"\u003e\u003c\/a\u003e\u003cem\u003e\u003c\/em\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/ilexlife.com\/products\/isokine-scf-human-animal-free-recombinant-protein\" title=\"ISOkine® SCF Human, Animal-Free Recombinant Protein\" target=\"_blank\"\u003eISOkine® SCF Human, Animal-Free Recombinant Protein\u003c\/a\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/stem-cell-factor-scf-human-e-coli-recombinant-protein\" title=\"Stem Cell Factor (SCF) Human, E. coli Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eSCF Human, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/stem-cell-factor-scf-human-sf9-recombinant-protein\" title=\"Stem Cell Factor (SCF) Human, Sf9 Insect Cells Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eSCF Human, Sf9 Insect Cells Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Stem Cell Factor (SCF) Human, HEK Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/stem-cell-factor-scf-human-hek-recombinant-protein\" target=\"_blank\"\u003eSCF Human, HEK Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/stem-cell-factor-scf-mouse-e-coli-recombinant-protein\" title=\"Stem Cell Factor (SCF) Mouse, E. coli Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eSCF Mouse, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Stem Cell Factor (SCF) Rat, E. coli Recombinant Protein\" href=\"https:\/\/ilexlife.com\/products\/stem-cell-factor-scf-rat-e-coli-recombinant-protein\" target=\"_blank\"\u003eSCF Rat, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/stem-cell-factor-scf-canine-e-coli-recombinant-protein\" title=\"Stem Cell Factor (SCF) Canine, E. coli Recombinant Protein\" rel=\"noopener\" target=\"_blank\"\u003eSCF Canine, E. coli Recombinant Protein\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors \u0026amp; cytokines manufactured by ORF Genetics Ltd.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"10 µg - Cat. No. IK2501-0010","offer_id":47853330432212,"sku":"IK2501-0010","price":170.0,"currency_code":"USD","in_stock":true},{"title":"50 µg - Cat. No. IK2501-0050","offer_id":47853330464980,"sku":"IK2501-0050","price":444.0,"currency_code":"USD","in_stock":true},{"title":"100 µg - Cat. No. IK2501-0100","offer_id":47853330497748,"sku":"IK2501-0100","price":684.0,"currency_code":"USD","in_stock":true},{"title":"1000 µg - Cat. No. IK2501-1000","offer_id":47853330530516,"sku":"IK2501-1000","price":3072.0,"currency_code":"USD","in_stock":true},{"title":"2000 µg - Cat. No. IK2501-2000","offer_id":47853330563284,"sku":"IK2501-2000","price":5460.0,"currency_code":"USD","in_stock":true},{"title":"5000 µg - Cat. No. IK2501-5000","offer_id":47853330596052,"sku":"IK2501-5000","price":12272.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/ISOkine_SCF_mouse.png?v=1777490319","url":"https:\/\/ilexlife.com\/products\/isokine-scf-mouse-animal-free-recombinant-protein","provider":"Ilex Life Sciences","version":"1.0","type":"link"}