{"product_id":"metatissue-hpcma-human-methacryloyl-placenta-photopolymerizable-hydrogel","title":"Metatissue® hPCMA - Human Methacryloyl Placenta Photopolymerizable Hydrogel","description":"\u003ch3 style=\"text-align: left;\"\u003e\u003cspan\u003eA human-derived and collagen-rich hydrogel platform!\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/metatissue-logo_160x160.png?v=1739385429\" alt=\"Metatissue Logo\"\u003e\u003c\/div\u003e\n\u003ch2\u003eIntroduction\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003e\u003cstrong\u003eMetatissue® hPCMA Photopolymerizable Hydrogel\u003c\/strong\u003e\u003c\/em\u003e is a human based, xeno-free, bioactive placenta-based platform that provides physiologically relevant microenvironments for cells culture. The precursor solution is easily prepared by reconstituting hPCMA lyophilizate in an aqueous solution with a photoinitiator. Afterwards, it can be cured upon light exposure to form a hydrogel with tuneable mechanical properties. hPCMA Photopolymerizable Hydrogels provide great control over gelation time and matrix stiffness, enabling researchers to create the cell culture environment that better fits their needs.\u003c\/span\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch3\u003eAdvantages\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHuman-derived: \u003c\/strong\u003eMultiple native human-derived extracellular matrix proteins provide a xeno-free alternative to animal products.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMechanically tuneable: \u003c\/strong\u003eMechanical properties can be easily adjusted to obtain several tissue stiffness simulations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePhotocrosslinkable: \u003c\/strong\u003eThe precursor solution can be cured upon light exposure to induce hydrogel crosslinking. Its temperature stability allows for easier handling.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile: \u003c\/strong\u003eEasily processed in multiple geometries and in microarrays amenable to organ-on-a-chip systems designed for high throughput screening applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReproducible:\u003c\/strong\u003e The materials are prepared from multiple donor units that are pooled to minimize lot-to-lot variation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ehPCMA Material\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cem\u003eMetatissue® hPCMA Photopolymerizable Hydrogel \u003c\/em\u003eis composed of several extracellular matrix (ECM) proteins, including multiple collagen types (e.g., I, III, IV, and VI), as well as fibrinogen, laminin, fibrillin, and fibronectin. These bioactive components are well recognized as key constituents of the extracellular matrix, playing crucial roles in supporting cell adhesion, growth, and proliferation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMain Components:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCollagen Type I, III, IV, and VI\u003c\/li\u003e\n\u003cli\u003eLaminin\u003c\/li\u003e\n\u003cli\u003eFibronectin\u003c\/li\u003e\n\u003cli\u003eFibrinogen\u003c\/li\u003e\n\u003cli\u003eFibrillin\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ehPCMA is manufactured from human placentas (hPCs) obtained from licensed healthcare establishments after the donor’s informed consent. All placenta donations are compliant with the Convention on Human Rights and Biomedicine, and Data Protection Regulation. It is essential to observe universal precautions for handling and disposing of biological products while working with hPCMA.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePhotoinitiated Polymerization:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ehPCMA is chemically modified with photoresponsive groups to polymerize only when exposed to ultraviolet (UV) or visible light. Therefore, a \u003ca rel=\"noopener\" title=\"Metatissue® LAP Photoinitiator\" href=\"https:\/\/ilexlife.com\/products\/metatissue-lap-photoinitiator\" target=\"_blank\"\u003ephotoinitiator\u003c\/a\u003e is required to initiate the polymerization process (not provided). For recommended photoinitiators, please refer to the Instructions for Use (IFU).\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable width=\"100%\" style=\"width: 99.9987%; height: 176.25px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 34.3451%; height: 19.5833px;\"\u003e\u003cstrong\u003eProduct Category:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 65.5511%; height: 19.5833px;\"\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/hydrogels-scaffolds-biomatrices\" title=\"Hydrogels, scaffolds, biomatrices\" rel=\"noopener\" target=\"_blank\"\u003eHydrogels, scaffolds, biomatrices\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 34.3451%; height: 19.5833px;\"\u003e\n\u003cstrong\u003eProduct Type:\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 65.5511%; height: 19.5833px;\"\u003eHydrogel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 34.3451%; height: 19.5833px;\"\u003e\u003cstrong\u003eLiquid\/Powder:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 65.5511%; height: 19.5833px;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 34.3451%; height: 19.5833px;\"\u003e\u003cstrong\u003eSize:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 65.5511%; height: 19.5833px;\"\u003e10 mg or 100 mg vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 34.3451%; height: 19.5833px;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 65.5511%; height: 19.5833px;\"\u003e2-8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 34.3451%; height: 19.5833px;\"\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 65.5511%; height: 19.5833px;\"\u003e\u003ca href=\"https:\/\/ilexlife.com\/collections\/metatissue-human-protein-based-biomaterials\" title=\"Metatissue® Human Protein-Based Biomaterials\" rel=\"noopener\" target=\"_blank\"\u003eMetatissue®\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5833px;\"\u003e\n\u003ctd style=\"width: 34.3451%; height: 19.5833px;\"\u003e\u003cstrong\u003eCountry of Origin:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 65.5511%; height: 19.5833px;\"\u003ePortugal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e\u003cspan\u003ehPCMA Validation in Cell Culture\u003c\/span\u003e\u003c\/h2\u003e\n\u003ch3\u003e\u003cspan\u003eValidated Cell Types\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThese are some of the human cells already validated with the hPCMA product:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003eBone Marrow\u003c\/span\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eMesenchymal Stem Cells (BM-MSC)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eHuman Umbilical Vein Endothelial Cells (HUVEC)\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eCardiac\u003c\/span\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eHuman Induced Pluripotent Stem Cells-derived Cardiomyocytes (iPSC-CM)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eHuman Cardiac Fibroblasts (hCF)\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eNeuronal\u003c\/span\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003ePrimary murine forebrain neurons\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eVascular\u003c\/span\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eHuman Induced Pluripotent Stem Cells-derived Vascular population (EC, PC, SMC)\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cspan\u003eTunable Stiffness\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003ehPCMA Photopolymerizable Hydrogel has tunable mechanical properties adjusted by the concentration of hPCMA lyophilizate in the precursor solution. It is therefore possible to obtain hydrogels with different stiffness to closely mimic the mechanical properties of various native tissues.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg alt=\"hPCMA Photopolymerizable Hydrogel has tunable mechanical properties adjusted by the concentration of hPCMA lyophilizate in the precursor solution. It is therefore possible to obtain hydrogels with different stiffness to closely mimic the mechanical properties of various native tissues.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/hPCMA_stiffness.png?v=1778093442\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\n\u003ch3\u003e\u003cspan\u003eCell Viability \u0026amp; Proliferation\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eSeveral types of encapsulated cells, namely, human adipose-derived stem cells (hASCs), HepG2 cells (human liver cancer cell line) and SW982 cells (synovial sarcoma cell line) maintain their viability for at least 7 days and are able to proliferate inside the hydrogels.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/hPCMA_fig1.png?v=1778094361\" alt=\"Figure 1. Representative fluorescence images for HepG2\u0026amp;nbsp;cells encapsulated in hPCMA 0.7% w\/v (live\/dead assay).\u0026amp;nbsp;Scale bar: 500 μm.\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e Representative fluorescence images for HepG2 cells encapsulated in hPCMA 0.7% w\/v (live\/dead assay). Scale bar: 500 μm.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/hPCMA_fig2.png?v=1778094361\" alt=\"Figure 2. Representative fluorescence images for hASCs\u0026amp;nbsp;encapsulated in hPCMA 1% w\/v. Live\/dead assay - scale\u0026amp;nbsp;bar: 200 μm, and DAPI\/Phalloidin assay - scale bar: 20 μm.\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e Representative fluorescence images for hASCs encapsulated in hPCMA 1% w\/v. Live\/dead assay - scale bar: 200 μm, and DAPI\/Phalloidin assay - scale bar: 20 μm.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 3. Representative fluorescence images for SW982\u0026amp;nbsp;cells encapsulated in hPCMA 0.7% w\/v and 1% w\/v\u0026amp;nbsp;(live\/dead assay). Scale bar: 200 μm.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/hPCMA_fig3.png?v=1778094361\"\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eFigure 3.\u003c\/strong\u003e Representative fluorescence images for SW982 cells encapsulated in hPCMA 0.7% w\/v and 1% w\/v (live\/dead assay). Scale bar: 200 μm.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/hPCMA_fig4.png?v=1778094361\" alt=\"Figure 4. Metabolic activity normalized with the DNS\u0026amp;nbsp;content of hASCs encapsulated in hPCMA 1% w\/v, at 1, 3,\u0026amp;nbsp;and 7 days of culture. Results shown as mean (SD).\"\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eFigure 4.\u003c\/strong\u003e Metabolic activity normalized with the DNS content of hASCs encapsulated in hPCMA 1% w\/v, at 1, 3, and 7 days of culture. Results shown as mean (SD).\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 5. Relative metabolic activity of SW982 cells\u0026amp;nbsp;encapsulated in hPCMA 0.7% w\/v and 1% w\/v, at 0, 3, and 7 days of culture. Results shown as mean (SD).\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/hPCMA_fig5.png?v=1778094361\"\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eFigure 5.\u003c\/strong\u003e Relative metabolic activity of SW982 cells encapsulated in hPCMA 0.7% w\/v and 1% w\/v, at 0, 3, and 7 days of culture. Results shown as mean (SD).\u003c\/em\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003e\u003cspan\u003e3D Bioprinting\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003ehPCMA can be used as an ink for 3D printing in different printing techniques, such as DLP and volumetric bioprinting. It can also be added to new ink formulations as a complement for a human touch.\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/hPCMA_bioprinting.png?v=1778095074\" alt=\"hPCMA can be used as an ink for 3D printing in different printing techniques, such as DLP and\u0026amp;nbsp;volumetric bioprinting. It can also be added to new ink formulations as a complement for a human\u0026amp;nbsp;touch.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"Product Datasheet (PDS): Metatissue® hPCMA - Human Methacryloyl Placenta Photopolymerizable Hydrogel\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/PC-TD-019_01-ShareP-hPCMA_Product-data-sheet.pdf?v=1778091710\" target=\"_blank\"\u003eProduct Datasheet (PDS)\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/Product_Spec_Sheet_Graphene_Foam_-_Ilex.pdf?v=1722537853\" title=\"Product Data Sheet (PDS): 3D G-Scaffold - Graphene foam scaffold for conductive 3D cell culture\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/PC-TD-010_04-ShareP-hPCMA-_Instructions-for-use.pdf?v=1778091710\" title=\"Instruction For Use (IFU): Metatissue® hPCMA - Human Methacryloyl Placenta Photopolymerizable Hydrogel\" rel=\"noopener\" target=\"_blank\"\u003eInstructions For Use (IFU)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Material Safety Data Sheet (MSDS): Metatissue® hPCMA - Human Methacryloyl Placenta Photopolymerizable Hydrogel\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/PC-TD-018_01-ShareP-hPCMA-SDS.pdf?v=1778091710\" target=\"_blank\"\u003eMaterial Safety Data Sheet (MSDS)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eUsage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is intended for \u003c\/span\u003e\u003cstrong\u003eLaboratory Research Use Only\u003c\/strong\u003e\u003cspan\u003e. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Metatissue® LAP Photoinitiator\" href=\"https:\/\/ilexlife.com\/products\/metatissue-lap-photoinitiator\" target=\"_blank\"\u003e\u003cspan\u003eMetatissue® LAP Photoinitiator\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/metatissue-hplma-human-methacryloyl-platelet-lysates-photopolymerizable-hydrogel\" title=\"Metatissue® hPLMA - Human Methacryloyl Platelet Lysates Photopolymerizable Hydrogel\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003eMetatissue® hPLMA - Human Methacryloyl Platelet Lysates Photopolymerizable Hydrogel\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ilexlife.com\/products\/metatissue-hplma-easy-hydrogel-human-methacryloyl-platelet-lysates-solution-with-lap-photoinitiator\" title=\"Metatissue® hPLMA Easy Hydrogel - Solution Premixed with LAP Photoinitiator\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003eMetatissue® hPLMA Easy Hydrogel - Solution Premixed with LAP Photoinitiator\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Metatissue® hPLMA Sponge - Scaffold for 96-well 3D Cell Culture\" href=\"https:\/\/ilexlife.com\/products\/metatissue-hplma-sponge-scaffold-for-96-well-3d-cell-culture\" target=\"_blank\"\u003e\u003cspan\u003eMetatissue® hPLMA Sponge - Scaffold for 96-well 3D Cell Culture\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eIlex Life Sciences LLC is an authorized worldwide distributor of Metatissue® products.\u003c\/em\u003e\u003c\/p\u003e","brand":"Ilex Life Sciences","offers":[{"title":"10 mg - Cat. No. PC01\/010","offer_id":47896056266964,"sku":"PC01\/010","price":285.0,"currency_code":"USD","in_stock":true},{"title":"100 mg - Cat. No. PC01\/100","offer_id":47896300388564,"sku":"PC01\/100","price":880.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0563\/4487\/1064\/files\/hPCMA.png?v=1778097123","url":"https:\/\/ilexlife.com\/products\/metatissue-hpcma-human-methacryloyl-placenta-photopolymerizable-hydrogel","provider":"Ilex Life Sciences","version":"1.0","type":"link"}