Description
Beta Lactamase, E. coli Recombinant Protein is a single, non-glycosylated polypeptide chain that contains 264 amino acids and has a molecular mass of approximately 28.9 kDa. Beta Lactamase is purified by proprietary chromatographic techniques.
Summary
Beta-lactamase is a type of enzyme (EC 3.5.2.6) produced by some bacteria that is responsible for their resistance to beta-lactam antibiotics like penicillins, cephalosporins, cephamycins and carbapenems. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties.
Other names
beta-lactamase, b-lactamase, β-Lactamase, LACTB, TEM-1
Type
Recombinant protein
Source
E. coli
Amino Acid Sequence
MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
One unit will hydrolyze 1.0 μmole of benzyl penicillin at pH 7.0 at 25°C, in presence of EDTA.
Formulation
Sterile-filtered, white lyophilized (freeze-dried) powder. Lyophilized from a concentrated solution in 100 mM Tris, pH 7.0.
Reconstitution
It is recommended to reconstitute the lyophilized Beta Lactamase in sterile 18MΩ-cm H2O at a concentration of 100 µg/ml, which can then be further diluted to other aqueous solutions. The Beta Lactamase should be used in pH 7.0- 8.0 and in temperature not higher then 45°C.
Shipping
Ships at ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized Beta Lactamase, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Beta Lactamase Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.