Bring natural bone environments to the laboratory
What are P3D Scaffolds?
Ossiform has developed P3D Scaffolds – a uniquely lifelike bone environment made from β-tricalcium phosphate (β-TCP) – using a patented 3D printing process. P3D scaffolds are three-dimensional (3D) cell growth support structures and tissue models made of natural, biocompatible materials.
These lifelike bone models represent a reliable research model for studying bone disease mechanisms and testing new treatments such as drug- or cell therapy candidates. With the capacity to overcome the shortcomings and drawbacks of conventional 2D cellular models and animal models, the P3D Scaffolds show huge potential in research areas such as tissue engineering, disease modeling, regenerative medicine, drug screening, and stem cell and cancer research.
The structures are 3D printed with internal porosities to mimic the porous structure observed in human bones. In consequence, the lifelike models provide a more accurate replica of the bone composition and -stiffness, and therefore facilitates a more realistic growth.
The scaffold can also be used to test new therapies in lifelike structures wherein drug perfusion is uneven and where bacteria and cancer cells may hide in pores. This allows for a more realistic testing that is more likely to yield reliable results when subsequently translated.
- Enable complex co-culturing
- Maintain relevant cell signaling
- Support the natural self-organization of cells
- Easily apply 2D analysis methods
- For in vitro and in vivo pre-clinical research
- All P3D Scaffolds are supplied sterile and ready to use
- 100% polymer-free
- Consist solely of β-tricalcium phosphate (β-TCP)
- Clinically relevant environment
- Directly translate from in vitro to in vivo
- Fully customizable to your research needs
- Easy-to-use 3D cell culture and drug screening systems
Explore P3D Scaffolds - Standard Design Options
Gyroid or grid
Different infill types fulfill your different research needs.
Do you need to monitor cell morphology close while the cells are still in culture? Then go for the grid structure, which allows light to pass through the scaffold easily, thereby enabling you to perform regular checkups on your cells. Our data shows that cells will grow from the corners and in.
Is your research more focused on gaining a model system as natural as possible? Then the gyroid structure is just the right choice for you. Here you will get a randomized organization of the pores, resulting in a variable pore size, making regular checkups while in culture a bit trickier, but leaving you with a more accurate representation of the bone structure.
- Porosity 1: 300-400 micrometers
- Porosity 2: 600-800 micrometers
Four standard scaffold diameters are available, designed to fit common cell culture plate sizes. All standard P3D Scaffolds have a height of 3 mm.
- 6 mm diameter: suitable for 96-well plates
- 12 mm diameter: suitable for 24-well plates
- 20 mm diameter: suitable for 12-well plates
- 30 mm diameter: suitable for 6-well plates
Extra customizable features
Special forms, sizes (height, diameter, etc.), and designs of the scaffolds can be requested for a fully customized set of cell culture systems. Please contact us to discuss your specific needs.
- P3D Scaffolds Product Brochure
- P3D Scaffolds Frequently Asked Questions (FAQ)
- P3D Scaffolds Material Safety Data Sheet (MSDS)
- Protocol: P3D Scaffold Cell Recovery by Trypsination
- This protocol explains how to retrieve cells that have been cultured on the P3D Scaffolds. Cell recovery from the P3D Scaffolds can be performed using standard protocols with minor modifications.
- Protocol: How to Culture hMSCs in Bioceramic 3D Cell Culture Systems
- This protocol is for seeding and 3D culturing human mesenchymal stem cells (hMSCs) on P3D Scaffolds. The procedure provides guidelines on how to create three-dimensional multicellular in vitro tissue constructs based on hMSCs and subsequently differentiate the hMSCs into osteoblasts to create a 3D bone cell culture.
- Protocol: How to Verify Osteogenesis on P3D Scaffolds
- This guide is for studying osteoblast differentiation using an ALP staining assay. Mesenchymal stem cells express alkaline phosphatase (ALP), which increases during osteoblast differentiation. ALP levels can therefore be used as an osteogenic marker.
Ilex Life Sciences is an official distributor of Ossiform P3D Scaffolds research product line.