The Biocolor Fastin™ Elastin Assay is a quantitative dye-binding method for the analysis of elastins released into tissue culture medium and extracted from biological materials.
The dye label employed is 5,10,15,20-tetraphenyl-21H,23H-porphine tetra-sulfonate (TPPS).
Elastin forms that can be measured by the Fastin Assay as α-elastin:
- soluble tropoelastins;
- lathyrogenic elastins;
- insoluble elastins (following solubilization to elastin polypeptides [α-elastin, κ-elastin])
The dye reagent binds to the ‘basic’ and ‘non-polar’ amino acid sequences found in mammalian elastins. For analysis of soluble elastin, samples should be free of any particulate material (cell debris, insoluble extracellular matrix material). The presence of other soluble proteins or of complex carbohydrates does not interfere with the Fastin Elastin Assay.
- In Vitro Studies – soluble elastin secreted into cell culture medium may be measured directly. Samples should be free from any particulate material. Tropoelastin is the elastin form usually found in cell-culture medium during in vitro cell culture.
- In Vivo Studies – a method for the extraction of insoluble elastin from tissue and conversion to soluble α-elastin or soluble κ-elastin is given (page 12-13). After solubilisation the samples are assayed by the procedure described for soluble elastin.
Assay Run Time
Work Flow Chart
soluble tropoelastins, lathyrogenic elastins, Fastin elastin
In the links below you will find the Fastin™ Elastin Assay protocol and the Fastin™ Elastin Assay MSDS.
Ilex Life Sciences LLC is an official distributor of Biocolor products.