The Biocolor Fastin™ Elastin Assay is a quantitative dye-binding method for the analysis of elastins released into tissue culture medium and extracted from biological materials.
The dye label employed is 5,10,15,20-tetraphenyl-21H,23H-porphine tetra-sulfonate (TPPS).
Elastin forms that can be measured by the Fastin Assay as α-elastin:
- soluble tropoelastins;
- lathyrogenic elastins;
- insoluble elastins (following solubilization to elastin polypeptides [α-elastin, κ-elastin])
The dye reagent binds to the ‘basic’ and ‘non-polar’ amino acid sequences found in mammalian elastins. For analysis of soluble elastin, samples should be free of any particulate material (cell debris, insoluble extracellular matrix material). The presence of other soluble proteins or of complex carbohydrates does not interfere with the Fastin Elastin Assay.
Colorimetric Assay (513 nm)
In-vivo: tissues and fluids. Insoluble elastin will first require conversion to water soluble α-elastin using the oxalic acid reagents and extraction protocol supplied with the kit.
In-vitro: Elastin produced by cells during 2D/3D cell culture. NB elastin in conditioned cell media is typically below the detection limit of the kit.
|Assay Range||0 - 500 µg/ml|
|Limit of Detection||50 µg/ml|
|Assay Run-Time||4 hours|
Work Flow Chart
soluble tropoelastins, lathyrogenic elastins, Fastin Elastin
Ilex Life Sciences LLC is an official distributor of Biocolor products.