F2000 Biocolor Fastin™ Elastin Assay - Standard Size Kit (110 assays)
Fastin Elastin Assay, Large Economy Size Kit (440 assays)
Fastin Elastin Dye Dissociation Reagent (3 x 110 ml)
Fastin Elastin Reference Standard (1 mg/ml; 3 x 5 ml)
Fastin Elastin Dye Dissociation Reagent (3 x 110 ml)
  • Load image into Gallery viewer, F2000 Biocolor Fastin™ Elastin Assay - Standard Size Kit (110 assays)
  • Load image into Gallery viewer, Fastin Elastin Assay, Large Economy Size Kit (440 assays)
  • Load image into Gallery viewer, Fastin Elastin Dye Dissociation Reagent (3 x 110 ml)
  • Load image into Gallery viewer, Fastin Elastin Reference Standard (1 mg/ml; 3 x 5 ml)
  • Load image into Gallery viewer, Fastin Elastin Dye Dissociation Reagent (3 x 110 ml)

Biocolor Fastin™ Elastin Assay

F2000
Regular price
$640.00
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$640.00
Regular price
$719.00
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Description

The Biocolor Fastin™ Elastin Assay is a quantitative dye-binding method for the analysis of elastins released into tissue culture medium and extracted from biological materials.

The dye label employed is 5,10,15,20-tetraphenyl-21H,23H-porphine tetra-sulfonate (TPPS).

Elastin forms that can be measured by the Fastin Assay as α-elastin:

  1. soluble tropoelastins;
  2. lathyrogenic elastins;
  3. insoluble elastins (following solubilization to elastin polypeptides [α-elastin, κ-elastin])

The dye reagent binds to the ‘basic’ and ‘non-polar’ amino acid sequences found in mammalian elastins.  For analysis of soluble elastin, samples should be free of any particulate material (cell debris, insoluble extracellular matrix material).  The presence of other soluble proteins or of complex carbohydrates does not interfere with the Fastin Elastin Assay.

Type

Colormetric Assay

Applications

F2000 Biocolor Fastin Elastin Assay Kit (Standard size kit, 110 assays)
  1. In Vitro Studies – soluble elastin secreted into cell culture medium may be measured directly. Samples should be free from any particulate material. Tropoelastin is the elastin form usually found in cell-culture medium during in vitro cell culture.
  2. In Vivo Studies – a method for the extraction of insoluble elastin from tissue and conversion to soluble α-elastin or soluble κ-elastin is given (page 12-13). After solubilisation the samples are assayed by the procedure described for soluble elastin.

Sensitivity

5 µg

Assay Run Time

4 hours

Work Flow Chart

 

Fastin Elastin Assay flow chart to determine the protocol for your specific application.

 

Other Names/Synonyms

soluble tropoelastins, lathyrogenic elastins, Fastin elastin

Resources

In the links below you will find the Fastin™ Elastin Assay protocol and the Fastin™ Elastin Assay MSDS.

Ilex Life Sciences LLC is an official distributor of Biocolor products.