Human Chorionic Gonadotropin (hCG)
Human Chorionic Gonadotropin (hCG)
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Human Chorionic Gonadotropin (hCG)

A225005
Regular price
$245.00
Sale price
$245.00
Regular price
$282.00
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Description

Our bioactivity tested Human Chorionic Gonadotropin (hCG) is produced from a sterile preparation of placental glucoprotein urine of pregnant women and has a total molecular mass of 36,700 Dalton. The hCG consists of 237 amino acids, a chain-92 amino acids and b chain-145 amino acids. Our hCG is purified by proprietary chromatographic techniques.

Human chorionic gonadotropin (hCG) is a peptide hormone produced in pregnancy, that is made by the embryo soon after conception and later by the syncytiotrophoblast (part of the placenta). Its role is to prevent the disintegration of the corpus luteum of the ovary and thereby maintain progesterone production that is critical for a pregnancy in humans. hCG may have additional functions, for instance it is thought that it affects the immune tolerance of the pregnancy. Early pregnancy testing generally is based on the detection or measurement of hCG.

Type

Native Protein

Source

Our hCG is purified from urine of pregnant women

Formulation

Our hCG is lyophilized with no additives

Reconstitution

It is recommended to reconstitute the lyophilized Human Chorionic Gonadotropin in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions

Biological Activity

The hCG bioactivity was found to be 5625 IU/mg (will vary slightly between each lot, please refer to the datasheet enclosed with your shipment).

Shipping

At ambient temperature.  Upon receipt, store the product at -18ºC

Storage/Expiration

Lyophilized hCG although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution hCG should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles

Contaminants

Our hCG is free of: HbsAg and antibodies to HIV and HCV.

Usage

This product is intended to Laboratory Research Use Only.  This product may not be used a drug, agricultural product, food additives.

Resources

Below you will find links to our Mouse Superovulation Protocol and Protocol for Inducing Ovulation in Xenopus laevis:

Application

Our hCG is commonly used in combination with our Pregnant Mare Serum Gonadotropin (PMSG, eCG) in superovulation protocols for the production of genetically modified laboratory animals, such as genetically engineered mouse models (GEMM) by pronuclear microinjection (transgenic mouse), targeted mutations through CRISPR-Cas9 methods, and embryonic stem (ES) cell-based gene targeting to produce "knockout" mice. hCG also has applications with a variety of other species; for example, it may be used in the generation of Xenopus laevis and Xenopus tropicalis eggs or embryos for use in experimental model systems.

Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice.

Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice. Nakagawa, Y., Sakuma, T., Nishimichi, N., Yokosaki, Y., Yanaka, N., Takeo, T., Nakagata, N., & Yamamoto, T. (2016). Biology open5(8), 1142–1148. https://doi.org/10.1242/bio.019349