Our bioactive Pregnant Mare Serum Gonadotropin (PMSG), also known as Equine Chorionic Gonadotropin (eCG), is a complex 43–63 kda glycoprotein purified from the serum of pregnant mares.
Our PMSG hormone and Human Chorionic Gonadotropin (hCG) are commonly used in superovulation protocols for the production of genetically modified laboratory animals, such as genetically engineered mouse models (GEMM) by pronuclear microinjection (transgenic mouse), targeted mutations through CRISPR-Cas9 methods, and embryonic stem (ES) cell-based gene targeting to produce "knockout" mice. PMSG also has applications with a variety of other species; for example, it may be used in the generation of Xenopus laevis and Xenopus tropicalis eggs or embryos for use in experimental model systems.
Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice. Nakagawa, Y., Sakuma, T., Nishimichi, N., Yokosaki, Y., Yanaka, N., Takeo, T., Nakagata, N., & Yamamoto, T. (2016). Biology open, 5(8), 1142–1148. https://doi.org/10.1242/bio.019349
Equine Chorionic Gonadotropin, eCG, Pregnant Mare Serum Gonadotrophin, Pregnant Mare Serum Gn
CAS Registry Number
CAS No. 9002-70-4
Serum of pregnant mares
Sterile-filtered white lyophilized (freeze-dried) powder
Our PMSG is lyophilized with no additives
We recommend that lyophilized PMSG is reconstituted in sterile 18M-cm H2O at a concentration of 1000 IU/ml, which can then be further diluted into other aqueous solutions.
At ambient temperature. Upon receipt, store the product at 2-8ºC
Lyophilized PMSG although stable at room temperature for 3 weeks, should be stored between 2-8ºC. Once reconstituted it is recommended to aliquot and store at -70ºC for long term use.
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
PMSG hormone is commonly used to increase ovulation just before artificial insemination. PMSG hormone is a placental glycoprotein comprised of alpha and beta subunits produced from the serum of pregnant mares. PMSG is secreted from endometrial cups within the pregnant mare uterus aging from 40 to 130 days into their maturation, and once extracted, it can be used to promote artificial estrus in female animals. These assemblies produce PMSG hormone to induce a mare's ovarian and reproductive structures. PMSG can induce the growth of follicles by ovaries and results in ovulation. PMSG hormone has a bioactive half-life of about 4 days in species other than horses. The extended biological activity can cause ovarian stimulation and ovulation and is often used in superovulation protocols. PMSG is more likely to be used than other pituitary hormones due to the extended circulatory half-life. PMSG solely exhibits luteinizing hormone (LH) like activity, however, in other animal classes gonadotropin has follicle-stimulating hormone (FSH) and LH-like activity.
Below you will find our protocols and other relevant links:
- Protocol: Mouse Superovulation
- Protocol: Inducing Ovulation in Xenopus laevis
- NIH ChemIDplus: Substance Name: Gonadotropin, serum (CAS No. 9002-70-4)