Description
EndoTrap® is an affinity matrix intended for removal of lipopolysaccharide (LPS) from biological samples in aqueous solutions such as proteins, antibodies, cell extracts and nucleic acids.
This multipurpose product portfolio covers a broad range of conditions with no special equipment or buffer requirements.
Affinity chromatography for highly efficient endotoxin removal
- Removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate
- Helps avoid artefacts caused by endotoxin contamination in sensitive in-vitro and in-vivo assays
- Can be employed in both column or batch mode, by gravity flow or on fully automated liquid chromatography systems
EndoTrap® red can be employed in both batch and column mode and is specially developed for buffers containing calcium chelators and for low-salt conditions.
Features
| Endotoxin binding capacity | 2,000,000 EU/ml |
| Sample recovery rate | >95% |
| Toxicity | Non-toxic, no cytotoxic effects |
| Reusability | Re-use at least 3 times |
| Suitable for low-salt conditions? | Yes |
| pH sample range | 6-9 |
| Buffers | Suitable for PBS, HEPES, Borate, TRIS, MOPS, MES, PIPES, Citrate, Acetate, Glycine and Carbonate buffers |
| Format | Pre-filled gravity flow column or bulk resin |
Choosing between EndoTrap Red and HD
Buffer Compatibility
Endotoxin removal with EndoTrap® red works effectively in the pH range of 6-9 and NaCl concentrations in the range of 50-250 mM. Buffers such as HEPES, PBS, TRIS, MOPS, MES, PIPES and also Citrate, Acetate, Glycine and Carbonate buffers are recommended. EndoTrap® red can be used with chelators of divalent cations (like EDTA). Phosphate buffers with NaCl concentrations below 100 mM are recommended for EndoTrap® red.
Products Available in EndoTrap® red Family
| Product Name | Cat. No. | Description |
| EndoTrap® red 1/1 | LET0001 | 1 x 1 mL column, 25 mL 5x Equilibration Buffer, 25 mL 5x Regeneration Buffer |
| EndoTrap® red 5/1 | LET0002 | 5 x 1 mL column, 125 mL 5x Equilibration Buffer, 125 mL 5x Regeneration Buffer |
| EndoTrap® red 5 | LET0033 | 5 mL settled resin |
| EndoTrap® red 10 | LET0003 | 10 mL settled resin |
| EndoTrap® red 50 | LET0004 | 50 mL settled resin |
| EndoTrap® red Buffer Kit | LET0032 | 125 mL 5x Equilibration Buffer, 125 mL 5x Regeneration Buffer |
| Empty Columns Large | LET0019 | for 2-10 mL resin (pack of 5 columns) |
| Empty Columns Small | LET0020 | for 0.2-2 mL resin (pack of 5 columns) |
| Disposable Polypropylene Funnel | LET0018 | pack of 5 funnels |
| EndoGrade® Endotoxin-Free Glass Test Tubes (112 ct.) | LET0021 | 112 endotoxin-free borosilicate glass test tubes with aluminum screw cap, capacity 5 ml, height 50 mm |
Resources
- EndoTrap red Datasheet
- EndoTrap red Material Safety Data Sheet (MSDS)
- FAQ about EndoTrap
- EndoTrap User Applications
Related Products
Ilex Life Sciences LLC is an official distributor of LIONEX GmbH products.