EndoTrap® is an affinity matrix intended for removal of lipopolysaccharide (LPS) from biological samples in aqueous solutions such as proteins, antibodies, cell extracts and nucleic acids.
This multipurpose product portfolio covers a broad range of conditions with no special equipment or buffer requirements.
Affinity chromatography for highly efficient endotoxin removal
- Removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate
- Helps avoid artefacts caused by endotoxin contamination in sensitive in-vitro and in-vivo assays
- Can be employed in both column or batch mode, by gravity flow or on fully automated liquid chromatography systems
EndoTrap® red can be employed in both batch and column mode and is specially developed for buffers containing calcium chelators and for low-salt conditions.
|Endotoxin binding capacity||2,000,000 EU/ml|
|Sample recovery rate||>95%|
|Toxicity||Non-toxic, no cytotoxic effects|
|Reusability||Re-use at least 3 times|
|Suitable for low-salt conditions?||Yes|
|pH sample range||6-9|
|Buffers||Suitable for PBS, HEPES, Borate, TRIS, MOPS, MES, PIPES, Citrate, Acetate, Glycine and Carbonate buffers|
|Format||Pre-filled gravity flow column or bulk resin|
Endotoxin removal with EndoTrap® red works effectively in the pH range of 6-9 and NaCl concentrations in the range of 50-250 mM. Buffers such as HEPES, PBS, TRIS, MOPS, MES, PIPES and also Citrate, Acetate, Glycine and Carbonate buffers are recommended. EndoTrap® red can be used with chelators of divalent cations (like EDTA). Phosphate buffers with NaCl concentrations below 100 mM are recommended for EndoTrap® red.
Products Available in EndoTrap® HD Family
|Product Name||Cat. No.||Description|
|EndoTrap® red 1/1||LET0001||1 x 1 mL column, 25 mL 5x Equilibration Buffer, 25 mL 5x Regeneration Buffer|
|EndoTrap® red 5/1||LET0002||5 x 1 mL column, 125 mL 5x Equilibration Buffer, 125 mL 5x Regeneration Buffer|
|EndoTrap® red 5||LET0033||5 mL settled resin|
|EndoTrap® red 10||LET0003||10 mL settled resin|
|EndoTrap® red 50||LET0004||50 mL settled resin|
|EndoTrap® red Buffer Kit||LET0032||125 mL 5x Equilibration Buffer, 125 mL 5x Regeneration Buffer|
|Empty Columns Large||LET0019||for 2-10 mL resin (pack of 5 columns)|
|Empty Columns Small||LET0020||for 0.2-2 mL resin (pack of 5 columns)|
|Disposable Polypropylene Funnel||LET0018||pack of 5 funnels|
Ilex Life Sciences LLC is an official distributor of LIONEX GmbH products.