Description
The Biocolor Blyscan™ Glycosaminoglycan Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans, (sGAG).
Test material includes:
- Cell culture derived material
- Soluble sGAGs from fluids, such as synovial fluid, urine.
- Cartilage
- Connective tissue / tumors
Soluble sGAGs may be assayed directly, solid materials will require a preliminary papain extraction.
The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples.
The dye label used in the assay is 1,9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains.
Please note:
The assay is not suitable for small sulfated disaccharide fragments or for samples containing alginates, as these contain uronic acid.
Type
Colormetric Assay
Applications
- fibrous and hyaline cartilages
- arteries, heart, lung, skin and other material containing extracellular matrix (connective tissue), and solid tumors
- extracellular matrix components that may be released by live cells into culture medium
Sensitivity
0.5 µg
Assay Run Time
1 hour
Technical Video
Other Names/Synonyms
sGAG, s-glycosaminoglycan, sulfated glycosaminoglycan
Resources
Ilex Life Sciences LLC is an official distributor of Biocolor Ltd.