Description
The Biocolor Blyscan™ Glycosaminoglycan Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans, (sGAG).
Test material includes:
- Cell culture derived material
- Soluble sGAGs from fluids, such as synovial fluid, urine.
- Cartilage
- Connective tissue / tumors
Soluble sGAGs may be assayed directly, solid materials will require a preliminary papain extraction.
The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples.
The dye label used in the assay is 1,9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains.
Please note:
The assay is not suitable for small sulfated disaccharide fragments or for samples containing alginates, as these contain uronic acid.
Type
Colormetric Assay
Applications
- fibrous and hyaline cartilages
- arteries, heart, lung, skin and other material containing extracellular matrix (connective tissue), and solid tumors
- extracellular matrix components that may be released by live cells into culture medium
- soluble sGAG from synovial fluid, urine and gel chromatography fraction aliquots
Test Sample Composition
An extensive range of mammalian proteoglycans have been measured using this assay. The following sGAG, either still attached to the peptide/protein core, or as free chains can be assayed:
- chondroitin sulfates (4- & 6-sulfated)
- keratan sulfates (alkali sensitive & resistant forms)
- dermatan sulfate
- heparan sulfates (including heparins)
Hyaluronate is not detected by the Blyscan Assay. The presence of hyaluronate in test samples does not interfere with the measurement of sGAG.
Sensitivity
0.5 µg
Assay Run Time
1 hour
Technical Video
Other Names/Synonyms
sGAG, s-glycosaminoglycan, sulfated glycosaminoglycan
Resources
Ilex Life Sciences LLC is an official distributor of Biocolor Ltd.