Blyscan™ Glycosaminoglycan (sGAG) Assay, manufactured by Biocolor, distributed by Ilex Life Sciences.
Step-by-step Blyscan sGAG Assay procedure.
Biocolor Blyscan Dye & Dye Dissociation Reagent Pack - B1500
Blyscan sGAG Assay Flowchart
Blyscan™ Glycosaminoglycan (sGAG) Dye Dissociation Reagent
Blyscan™ Glycosaminoglycan (sGAG) Dye Reagent
Blyscan™ Glycosaminoglycan (sGAG) Reference Standard
Blyscan™ Glycosaminoglycan (sGAG) Assay, Large Economy Size Kit (440 Assays), manufactured by Biocolor, distributed by Ilex Life Sciences.

Biocolor Blyscan™ Glycosaminoglycan Assay

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Available January 20th
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The Biocolor Blyscan™ Glycosaminoglycan Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans, (sGAG).

Test material includes:

  • Cell culture derived material
  • Soluble sGAGs from fluids, such as synovial fluid, urine.
  • Cartilage
  • Connective tissue / tumors

Soluble sGAGs may be assayed directly, solid materials will require a preliminary papain extraction.

The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples.

The dye label used in the assay is 1,9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains.

Please note:

The assay is not suitable for small sulfated disaccharide fragments or for samples containing alginates, as these contain uronic acid.


Colormetric Assay

Blyscan™ Glycosaminoglycan (sGAG) Assay, manufactured by Biocolor, distributed by Ilex Life Sciences.


  • fibrous and hyaline cartilages
  • arteries, heart, lung, skin and other material containing extracellular matrix (connective tissue), and solid tumors
  • extracellular matrix components that may be released by live cells into culture medium
Blyscan sGAG Assay Flow Chart


0.5 µg

Assay Run Time

1 hour

Technical Video


Other Names/Synonyms

sGAG, s-glycosaminoglycan, sulfated glycosaminoglycan


Ilex Life Sciences LLC is an official distributor of Biocolor Ltd.