EndoTrap® HD Endotoxin Removal Products
EndoTrap® HD Endotoxin Removal Products
EndoTrap® HD Endotoxin Removal Products
EndoTrap® HD Endotoxin Removal Products
EndoTrap® HD Endotoxin Removal Products
EndoTrap® HD Endotoxin Removal Products
EndoTrap® HD Endotoxin Removal Products
EndoTrap® HD Endotoxin Removal Products
EndoTrap® HD Endotoxin Removal Products
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EndoTrap® HD Endotoxin Removal Products

LET0009
Regular price
$365.00
Sale price
$365.00
Regular price
$382.00
Temporarily out of stock
Unit price
per 
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Description

EndoTrap® HD is intended for in vitro quantitative removal of lipopolysaccharide (LPS) from biological samples in aqueous solutions such as proteins, antibodies, cell extracts and nucleic acids. EndoTrap® HD can be used in small scale processes for R&D and large scale processes, like manufacturing. It can be applied in early or late biomanufacturing process steps. EndoTrap® HD is based on hydrophilic, dimensionally stable affinity matrix with excellent pressure/flow characteristics.

Affinity chromatography for highly efficient endotoxin removal

  • Removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate
  • Helps avoid artefacts caused by endotoxin contamination in sensitive in-vitro and in-vivo assays
  • Fulfills requirements for industrial applications and can be easily integrated in early or late stage purification processes
  • Can be employed in both column or batch mode, by gravity flow or on fully automated liquid chromatography systems

Features

Endotoxin binding capacity
> 5,000,000 EU/mL
Endotoxin removal rate
> 99.99%
Sample recovery rate
> 95%
pH sample range
4-10
Toxicity
Non-toxic, no cytotoxic effects
Reusability
Re-use more than 10 times
Suitable for high-salt conditions?
Yes
Buffers
  • Suitable for TRIS, HEPES, MOPS or similar buffered samples
  • PBS buffer possible when enriched freshly with 0.1 mM calcium (magnesium) directly before use
Format Pre-filled gravity flow column, pre-filled FPLC column, or bulk resin

 

Choosing between EndoTrap Red and HD

Buffer Compatibility

Endotoxin removal with EndoTrap® HD works effectively in the pH range of 4-9 and NaCl concentrations of 50-600 mM. Buffers such as HEPES, TRIS, MOPS, MES, and PIPES are recommended. Citrate buffers and chelators of divalent cations (like EDTA) have to be avoided

Note: Customer specific buffers must contain 0.1 - 1 mM Ca2+ or Mg2+. When using PBS buffer always add 1 mM Ca2+ (e.g. CaCl2 ) and 1 mM Citrate pH 7 to the customer specific buffer. Otherwise phosphate and Ca2+ form an insoluble complex and will precipitate.

Leakage ELISA

EndoTrap® affinity chromatography is one principal method for cleaning biological solutions from endotoxin contaminations. The EndoTrap® ligand is a protein by nature, which is bound to the polymeric bead-matrix by stable covalent bonds. However, leakage of minute amounts of ligand is a matter of fact for all affinity materials and testing on these contaminants is often required for regulatory purposes. Depending on the intended use of the preparation and the step in the purification process (early or late), where EndoTrap® is used, a quantitative analysis of residual EndoTrap® ligand might be required. The EndoTrap® HD Leakage ELISA was developed to allow an accurate and reproducible determination of small amounts of EndoTrap® ligand in biological samples. This ELISA is suitable for detection of leached ligand from EndoTrap® HD resin.

Products Available in EndoTrap® HD Family

Product Name Cat. No.  Description
EndoTrap® HD 1/1 LET0009 1 x 1 mL column, 25 mL 5x Equilibration Buffer, 25 mL 5x Regeneration Buffer, 25 mL 5x Storage Buffer
EndoTrap® HD 5/1 LET0010 5 x 1 ml column, 125 mL 5x Equilibration Buffer, 125 mL 5x Regeneration Buffer, 125 mL 5x Storage Buffer
EndoTrap® HD 5 LET0023 5 mL settled resin
EndoTrap® HD 10 LET0011 10 mL settled resin
EndoTrap® HD 50 LET0012  50 mL settled resin
EndoTrap® HD 250 LET0013 250 mL settled resin
EndoTrap® HD FPLC Column (5 mL) LET-HD-FPLC5ML 5 mL resin in FPLC Column
EndoTrap® HD Buffer Kit LET0031 125 mL 5x Equilibration Buffer, 125 mL 5x Regeneration Buffer, 125 mL 5x Storage Buffer
EndoTrap® HD Leakage ELISA LET0014 96 determinations (12 x 8 well strip & frame)
Empty Columns Large
LET0019  for 2-10 mL resin (pack of 5 columns)
Empty Columns Small LET0020  for 0.2-2 mL resin (pack of 5 columns)
Disposable Polypropylene Funnel
LET0018  pack of 5 funnels

 

Resources

Ilex Life Sciences LLC is an official distributor of LIONEX GmbH products.