Introducing the Fastin Assay Kit: Your Straightforward Solution for Elastin Quantification! Our user-friendly kit utilizes a dye-based method to measure elastin from in-vivo and in-vitro sources. It can be used to quantify various elastin forms, spanning from immature tropoelastin to mature, 'insoluble' elastin fibers.
Description
The Biocolor Fastin™ Elastin Assay is a quantitative dye-binding method for the analysis of elastins released into tissue culture medium and extracted from biological materials.
The dye label employed is 5,10,15,20-tetraphenyl-21H,23H-porphine tetra-sulfonate (TPPS).
Elastin forms that can be measured by the Fastin Assay as α-elastin:
- soluble tropoelastins;
- lathyrogenic elastins;
- insoluble elastins (following solubilization to elastin polypeptides [α-elastin, κ-elastin])
The dye reagent binds to the ‘basic’ and ‘non-polar’ amino acid sequences found in mammalian elastins. For analysis of soluble elastin, samples should be free of any particulate material (cell debris, insoluble extracellular matrix material). The presence of other soluble proteins or of complex carbohydrates does not interfere with the Fastin Elastin Assay.
Principle of Method
Step 1. Incubation of samples containing soluble elastin with the Fastin Dye Reagent causes an elastin-dye complex to form. This insoluble complex then precipitates.
Step 2. Dye-labelled elastin is then isolated by centrifugation and the unbound dye removed. Elastin-bound dye is then eluted and measured spectrophotometrically.
Step 3. The elastin content of unknown samples can be calculated by comparison against a calibration curve prepared using a standard comprising water-soluble elastin (supplied with the kit).
Suitable Samples
In-vivo: tissues and fluids. Insoluble elastin will first require conversion to water soluble α-elastin using the oxalic acid reagents and extraction protocol supplied with the kit.
In-vitro: Elastin produced by cells during 2D/3D cell culture. NB elastin in conditioned cell media is typically below the detection limit of the kit.
Specifications
| Detection Method | Colorimetric Detection (513 nm) |
| Assay Range | 0 - 500 µg/ml |
| Limit of Detection | 50 µg/ml |
| Assay Run-Time | 4 hours |
| Measurements Per Kit | 110 in total (allows a maximum of 48 samples to be run in duplicate alongside a standard curve) |
Kit Contents
The standard size Fastin™ Elastin kit contains:
- Dye Reagent (1x110ml)
- α-elastin Reference Standard (1x5ml, 1.0 mg/ml soluble Bovine elastin)
- Oxalic Acid (1x20ml)
- Precipitating Reagent (1x30ml)
- Dye Dissociation Reagent (1x30ml)
- 1.5 ml micro-centrifuge tubes for dye-labelling reaction.
- Assay kit manual
Other Names/Synonyms
soluble tropoelastins, lathyrogenic elastins, Fastin Elastin
Resources
- Product Datasheet (PDS)
- Product References
- Material Safety Data Sheet (MSDS)
- Frequently Asked Question (FAQ)
Related Products
Ilex Life Sciences LLC is an official distributor of Biocolor products.