Picture of lyophilized vials of protein.

Carboxypeptidase B Rat, E. coli Recombinant Protein

A447505
$125.00
$125.00
$148.00
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Description

Carboxypeptidase B Rat, E. coli Recombinant Protein, also known as CPB, is a bioactive protease enzyme that preferentially cleaves off basic amino acids arginine and lysine from the C-terminus of a peptide. The enzyme is purified by standard chromatography techniques and supplied as a lyophilized powder. The CPB enzyme is free of foreign enzymes such as carboxypeptidase A and chymotrypsin, and also free of protease inhibitors such as PMSF and EDTA. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA, 1,10-phenanthroline.

Application

Carboxypeptidase B hydrolyzes the basic amino acids lysine (L), arginine (R), and ornithine from the C-terminal position of polypeptides.

Carboxypeptidase B is commonly used in:

  • Peptide mapping and protein sequencing
  • Determination of protein C-terminal amino acid
  • Sequence analysis by successive cleavage of C-terminal basic amino acids.
  • Insulin production by converting pro-insulin to insulin.
  • Production and quality control of monoclonal antibodies (mAbs).

Specifications

Product Category:

Proteins

Product Type:

Enzymes

CAS Registry No.:

9025-24-5

Source:

Expressed in E. coli.

Appearance:

Sterile-filtered, lyophilized power.

Formulation:

The protein was lyophilized with 100 mM NaCl, mannitol and 20 mM Tris pH-7.5.

Reconstitution:

It is recommended to reconstitute the lyophilized CPB in sterile 18MΩ-cm H2O or 25 mM Tris-HCl pH 7.65 not less than 100 µg/ml , which can then be further diluted to other aqueous solutions.

Purity:

Greater than 90% as determined by SDS-PAGE.

Biological Activity:

170 units/mg.

Unit Definition:

One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65.

Shipping Condition:

Shipped at ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage Condition:

Store the lyophilized CPB at 4°C. Upon reconstitution, the protein should be stored at 4°C for 2 weeks and for future use below -18°C.
Please prevent freeze-thaw cycles.

    Usage

    This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.

    Other Names

    Carboxypeptidase B, Carboxypeptidase-B, Cpb1, Cpb, EC:3.4.17.2, P19223, Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase.

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