Description
Bovine Collagen-VI is a natural protein purified from bovine placenta. Collagen-VI is purified by proprietary chromatographic techniques.
Summary
Collagen, a major component of the extracellular matrix (ECM), is a fibrous protein that provides tensile strength to tissues giving them structural integrity. Collagens contribute to mechanical properties, organization, and shape of tissues. Collagen is the most widely used ECM protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.
Collagen type VI is primarily associated with the extracellular matrix found in skeletal muscle. It maintains regularity in muscle function and stabilizes the cell membrane. It is synthesized by a complex, multistep pathway that leads to the formation of a unique network of linked microfilaments located in the extracellular matrix. It is linked to the genes COL6A1, COL6A2, and COL6A3. Malfunctions in this type of collagen are connected to both Bethlem myopathy and Ullrich congenital muscular dystrophy.
Source
Bovine placenta.
Formulation
Filtered white lyophilized (freeze-dried) powder. Collagen-VI was lyophilized without additives.
Purity
Purity > 90.0%.
Reconstitution
We recommend to add 0.5 M acetic acid, pH 2.5 to prepare a working stock solution not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized Collagen-VI, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Collagen-VI should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Other Names
Type VI Collagen, Type 6 Collagen, Collagen-6, COL6A1