Description
PAN-Biotech Endopan 300 SL is a serum-free medium designed for serum-free culture of endothelial cells directly after isolation. This exclusive product is optimized for the maintenance and expansion of endothelial cells under serum-free culture conditions.
| Cell Type: | HUVEC and Endothelial cells |
|---|---|
| Liquid / Powder: | liquid |
| Product Category: | Serum-Free Media |
| Sterile: | Yes |
Suitability
Endopan 300 SL is suitable for the culture of human umbilical vein endothelial cells (HUVEC), which are the cells of choice for studying function and pathology of endothelial cells (e.g. angiogenesis). They can be cultured continuously with a stable growth rate in serum-free media.
Figure 1: HUVEC cell growth over 14 days cultivation with Endopan SL 300.
Kit Components
In addition to the Endopan 300 SL Basal Medium, an Endopan 300 SL Kit is available.
Endopan 300 SL Kit is provided with serum substitute (Panexin SL-S, P04-0065S) and supplements in separate sterile packing. This will enable the user to prepare a medium for special applications. For example, VEGF, FGF-2, or other components may be omitted from the complete medium for specific experimental settings.
Endopan 300 SL Kit contains the following components in single packing:
- Basal Medium Endopan 300 SL
- Serum substitute Panexin SL-S
- EGF (human recombinant Epidermal Growth Factor)
- FGF-2 (human recombinant basic Fibroblast Growth Factor)
- VEGF (human recombinant Vascular Endothelial Growth Factor)
- Vitamin C (Ascorbic Acid phosphate)
- R3-IGF-1 (human recombinant Insulin-like Growth Factor)
- GA (Gentamycin/Amphotericin)
- Hydrocortisone
- Heparin
Storage conditions & stability
| Component |
Description |
Size | Storage |
Stability |
| Basal Medium (P04-0065B) | Endopan 300 SL Serum-free medium | 500 ml | 2-8°C | 2 years |
| Panexin SL-S | Serum replacement | 50 ml | -20°C | 6 months |
| EGF | Human epidermal growth factor, recombinant | 0.5 ml | -20°C | 1 year |
| FGF-2 | Human fibroblast growth factor, recombinant | 0.5 ml | -20°C | 1 year |
| VEGF | Human vascular endothelial growth factor, recombinant | 0.5 ml | -20°C | 1 year |
| R3-IGF-1 | Human insulin-like growth factor, recombinant | 0.5 ml | -20°C | 2 years |
| Vitamin C | Ascorbic acid phosphate | 0.5 ml | -20°C | 2 years |
| GA | Gentamicin / Amphotericin | 0.3 ml | -20°C | 2 years |
| Hydrocortisone | Hormone | 0.1 ml | -20°C | 2 years |
| Heparin | Anticoagulant | 0.5 ml | -20°C | 2 years |
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Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.