Description
Our bioactive Fibroblast Growth Factor 23 (FGF-23) Human, E. coli Recombinant Protein is a single, non-glycosylated polypeptide chain that contains a total of 228 amino acids and has a 22.5 kDa molecular mass. The FGF-23 is purified by proprietary chromatographic techniques.
Summary
FGF-23 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
FGF-23 inhibits renal tubular phosphate transport. The FGF-23 gene was identified by its mutations associated with autosomal dominant hypophosphatemic rickets (ADHR), an inherited phosphate wasting disorder. Abnormally high level expression of FGF-23 was found in oncogenic hypophosphatemic osteomalacia (OHO), a phenotypically similar disease caused by abnormal phosphate metabolism. FGF-23 mutations have also been shown to cause familial tumoral calcinosis with hyperphosphatemia. FGF-23 is a regulator of vitamin-D metabolism and negatively regulates osteoblast differentiation and matrix mineralization.
Other Names
Fibroblast Growth Factor-23, FGF23, FGF 23, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor, HYPF, UniProtKB# Q9GZV9.
Amino Acid Sequence
MYPNASPLLG SSWGGLIHLY TATARNSYHL QIHKNGHVDG APHQTIYSAL MIRSEDAGFV VITGVMSRRY LCMDFRGNIF GSHYFDPENC RFQHQTLENG YDVYHSPQYH FLVSLGRAKR AFLPGMNPPP YSQFLSRRNE IPLIHFNTPI PRRHTRSAED DSERDPLNVL KPRARMTPAP ASCSQELPSA EDNSPMASDP LGVVRGGRVN THAGGTGPEG CRPFAKFI.
Source
E. coli
Formulation
Sterile filtered white lyophilized (freeze-dried) powder. The FGF-23 protein (0.5 mg/ml) was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Purity
Purity greater than 95.0% as determined by SDS-PAGE.
Biological Activity
The biological activity of FGF-23 was measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.05-0.5 µg/ml in the presence of 5 µg/ml of Recombinant Mouse Klotho and 10 µg/ml of HPR.
Reconstitution
It is recommended to reconstitute the lyophilized FGF-23 in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
Ships at ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized FGF-23, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, FGF-23 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
Want to learn more about FGF-23? We have compiled the links below which contain information that you may find interesting.
- UniProt Knowledgebase: UniProtKB - Q9GZV9 · FGF23_HUMAN: Fibroblast growth factor 23
- National Library of Medicine: Gene ID: 8074: FGF23 fibroblast growth factor 23 [Homo sapiens (human)]