Description
Gelacell™ is a unique, 3D microfibrous scaffold specifically engineered for advanced in vitro 3D cell culture and tissue engineering applications. Designed as a non-woven, highly porous scaffold, Gelacell™ offers exceptional biocompatibility and non-toxicity across a variety of cell types.
Gelacell™ nanofibrous scaffolds are available in a variety of materials and formats.
Gelacell™ PHB HYDRO is a flagship nanofibrous scaffold in the Gelacell™ family, engineered for advanced 3D cell culture, tissue engineering, and wound healing research.
Its hydrophilic PHB fibers enhance media absorption and surface wettability, promoting cell attachment, proliferation, and tissue-like organization. The highly porous, interconnected network supports efficient nutrient diffusion and waste exchange, ensuring healthier, physiologically relevant cell growth compared to traditional 2D cultures. This hydrophilic 10 cm × 10 cm scaffold sheet made from PHB HYDRO (blend of 91% polyhydroxybutyrate [PHB] and 9% polyethylene glycol [PEG]) can be used directly or customized for multiwell plates, bioreactors, or other experimental setups. Combining biocompatibility, mechanical stability, and eco-friendly composition, Gelacell™ PHB HYDRO provides researchers and industrial developers with a reliable, scalable, and next-generation platform for 3D cell culture, drug discovery, and wound healing material applications.
Features
- 3D architecture with a substantial available surface area.
- Minimal modifications required for transitioning from 2D to 3D culture.
- Stable mechanical properties.
- 10 cm × 10 cm scaffold can be cut into desired shape and sizes.
- Flexibility of scaffolds facilitates easy handleability.
- Porous structure promotes cell migration and efficient diffusion of nutrients, solutes, and gases.
- Compatibility with a variety of cell lines and culture conditions.
- Polymeric scaffolds: biocompatibility, diversity, and widely accepted in cellular applications.
- Sterilized by Gamma irradiation and remains sterile until the pack is opened.
- Storage at room temperature.
Specifications
|
Catalog No.: |
GC0901RN-SC-C |
| Polymer/Material: |
Hydrophilic PHB HYDRO blend:
|
| Format: | 10x10 cm sheet (ideal for tissue engineering/organoid scaffolds) |
| Appearance (dry): | White |
| Appearance (swelled in water/PBS): | White (translucent) |
| Fiber Orientation: | Random |
| Mean Fiber Diameter: | 0.6 – 1.6 μm |
| Thickness: | 70 - 340 μm |
| Area Density: | 4.5 – 8.0 g/m^2 |
| Porosity: | 97% - 99% |
| Wettability: | Hydrophilic |
| Absorptivity in PBS (pH 7.4 at 37°C): | 700% - 1300% (The material was kept soaked for 24 hours in PBS at 37°C) |
| Degradation (in PBS at 37°C): | Neglible |
| pH (of PBS solution after 24 hours): | 7.3 – 7.5, without observable changes |
| % Elongation: | 18% - 55% |
| Elastic Modulus: | 3000 – 10000 kPa |
| Ultimate Tensile Strength: | 250 – 600 kPa |
| Manufacturer: | Gelatex Technologies |
| Country of Origin: | Estonia |
PHB vs PHB HYDRO
Gelacell™ PHB scaffolds are available as regular PHB or PHB HYDRO. PHB HYDRO is a special extra-hydrophilic material that uses a blend of 91% PHB and 9% PEG.
Water contact angle: PHB (left), PHB HYDRO (right)
Morphology of PHB HYDRO Scaffold
The morphology of fibrous scaffolds plays a pivotal role in its interaction with eukaryotic cells derived from humans and animals. The unique structure of microfibers shown in Figure 2 and Figure 3, mimicking the extracellular matrix, offers a high surface area, and interconnected porous network. This morphology provides an advantageous environment for cell adhesion, proliferation, and differentiation.
Biocompatibility of PHB HYDRO Scaffold
The scaffolds exhibit promising biocompatibility with various cell types, including HELA, HEK293, HUH7, HEPG2, U2OS, BHK21, and C2C12. Their biomimetic design and specialized polymer composition enhance cell-material interactions, supporting key processes like cellular attachment and spreading - crucial for cell growth and function. HUH7 cell lines show adherence, proliferation, and infiltration within the scaffold structure (as depicted in Figure 4 to Figure 5). This compatibility is vital across diverse tissue engineering applications, enabling these cell lines to thrive within the scaffold's microenvironment.
Storage & Stability
Sterility and performance properties are maintained when stored at room temperature, with a shelf life of two years from the date of manufacture.
Resources
Related Products
Ilex Life Sciences LLC is an authorized distributor of Gelatex Technologies' Gelacell™ products.