The Granzyme B Activity Assay Reagent Set (2 x 96 wells) enables quantitative measurement of active human granzyme B in biological samples (ex. conditioned cell culture media, cell extracts, body fluids, tissue extracts, and purified enzyme preparations). The assay also recognizes mouse granzyme B.
Researchers can now detect high and low ranges with a single, easy and sensitive assay, at a low cost per sample. Developed and manufactured in The Netherlands by QuickZyme Biosciences.
Conditioned cell culture media, cell extracts, body fluids (plasma, wound fluid), tissue extracts, and (purified) enzyme preparations
2x Non-coated 96-well microplate, 2x Coating antibody solution, 2x Granzyme B standard, 2x Detection enzyme, 2x Chromogenic substrate, Protocol.
0.01 ng/ml (24 h incubation)
0.05 - 10 ng/ml
This product ships on dry ice. Upon receipt, store the product at the temperature recommended below.
Antibody stock: Store at -20°C, avoid repeated freeze/thaw cycles.
Granzyme B standard: Store at -70°C, avoid repeated freeze/thaw cycles.
Detection enzyme: Store at -70°C, avoid repeated freeze/thaw cycles.
Chromogenic Substrate: Store at -20°C.
GZMB, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E.
The GZMB gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. (source).
Want to learn more about granzyme B? We have compiled the links below that contain information which you may find interesting:
- UniProt Knowledgebase: UniProtKB - P10144 (GRAB_HUMAN): Granzyme B
- PubChem Gene Summary: GZMB - granzyme B (human)
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