Interferon alpha 2b Human, E. coli Recombinant Protein, 100 ug vial
Interferon alpha 2b Human, E. coli Recombinant Protein, 1 mg vial
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Interferon alpha 2b Human, E. coli Recombinant Protein

A32050100
Regular price
$199.00
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$199.00
Regular price
$229.00
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Description

IFN-a 2b Human Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and has a molecular mass of 19400 Dalton. The difference between IFNA2A and IFNA2B is in the amino acid present at position 23. IFN-alpha 2a has a lysine at that position 23 while IFN-alpha 2b has arginine.

The IFN-alpha 2b gene was obtained from human leukocytes.

The IFN-a 2b is purified by proprietary chromatographic techniques.

Summary

IFN-alpha is produced by macrophages and has antiviral activities. IFN stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.

Amino Acid Sequence

MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE

Type

Recombinant Protein

Source

E. coli

Purity

Greater than 98.0%

Biological Activity

The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 260,000,000 IU/ mg.

Formulation

Sterile Filtered White lyophilized (freeze-dried) powder. Lyophilized from a (1 mg/ml) solution in containing 2.3 mg Sodium phosphate dibasic and 0.55 mg sodium phosphate monobasic buffer.

Reconstitution

It is recommended to reconstitute the lyophilized IFN-alpha 2b in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Shipping

Shipped at ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Lyophilized IFN although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Usage

This product is intended to Laboratory Research Use Only.  This product may not be used a drug, agricultural product, food additives.

Other Names

IFN-α 2b, IFN alpha 2b, IFNA, INFA2, MGC125764, MGC125765, Interferon alfa-2b

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