Description
Our bioactive Interleukin-1 beta (IL-1 beta) Human, E. coli Recombinant Protein is a non-glycosylated polypeptide chain that contains 153 amino acids and has a molecular mass of 17000 Dalton. The IL-1 beta is purified by proprietary chromatographic techniques.
Summary
IL-1 beta is a potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells.
Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. Acts as a sensor of S. pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection.
Other Names
Interleukin 1 beta, Interleukin-1β, Interleukin-1b, IL-1β, IL-1b, IL1B, leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, Catabolin, lymphocyte activating factor, UniProtKB# P01584
Source
Expressed in E. coli.
Formulation
Sterile filtered white lyophilized (freeze-dried) powder. IL-1 beta was lyophilized from a concentrated (1 mg/ml) sterile solution containing 50 mM Phosphate buffer pH 7.1 and 150 mM NaCl.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Val-Arg-Ser.
Purity
Greater than 98.0% as determined by:
- Analysis by RP-HPLC.
- Analysis by SDS-PAGE.
Protein Content
Protein quantitation was carried out by two independent methods:
- UV spectroscopy at 280 nm using the absorbency value of 0.631 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
- Analysis by RP-HPLC, using a calibrated solution of IL-1 beta Recombinant as a Reference Standard.
Biological Activity
The specific activity as determined in the test of augmentation of lymphocyte proliferation assay using mouse thymus was found to be 200,000,000 IU/mg.
Reconstitution
It is recommended to reconstitute the lyophilized IL-1 beta in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized IL-1 beta, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, IL-1 beta should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.