Description
Our bioactive Interleukin-1 beta (IL-1 beta) Mouse, E. coli Recombinant Protein is a non-glycosylated polypeptide chain that contains 153 amino acids and has a molecular mass of 17500 Dalton. The IL-1 beta is purified by proprietary chromatographic techniques.
Summary
IL-1 beta is a potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells.
Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. Acts as a sensor of S. pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection.
Other Names
Murine Interleukin 1 beta, Mouse Interleukin-1β, Interleukin-1b, IL-1β, IL-1b, IL1B, leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, Catabolin, lymphocyte activating factor, UniProtKB# P10749.
Source
Expressed in E. coli.
Formulation
Sterile filtered white lyophilized (freeze-dried) powder. Mouse IL-1 beta was lyophilized from a sterile filtered aqueous solution in 10 mM sodium phosphate, pH 7.5.
Amino Acid Sequence
MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQGEPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRFVFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
The activity as determined by the dose-dependent proliferation of mouse D10S cells is 0.9-1.3 pg/ml, corresponding to a Specific Activity of 1.1 x 106 Units/mg.
Reconstitution
It is recommended to reconstitute the lyophilized IL-1 beta in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized IL-1 beta, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, IL-1 beta should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.