Description
Our bioactive Interleukin-2 (IL-2) Human, E. coli Recombinant Protein is a single, non-glycosylated polypeptide chain containing 133 amino acids and has a molecular mass of 15.4 kDa. The IL-2 is purified by proprietary chromatographic techniques.
Summary
IL-2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Other Names
Interleukin 2, IL2, T-cell growth factor (TCGF), UniProtKB# P60568
Amino Acid Sequence
APTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSII STLT.
Source
E. coli
Formulation
Sterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a 0.2 μm filtered concentrated solution (1.0 mg/ml) in PBS, pH 5.0.
Purity
Greater than 97.0% as determined by:
- Analysis by RP-HPLC.
- Analysis by SDS-PAGE.
Biological Activity
The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 1x107 IU/mg.
Reconstitution
It is recommended to reconstitute the lyophilized Interleukin-2 in sterile distilled water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized IL-2, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, IL-2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
Want to learn more about FGF-acidic? We have compiled the links below which contain information that you may find interesting.
- UniProt Knowledgebase: UniProtKB - P60568 · IL2_HUMAN: Interleukin-2
- National Library of Medicine: Gene ID: 3558: IL2 interleukin 2 [Homo sapiens (human)]