Description
Our bioactive Interleukin-4 (IL-4) Rat, E. coli Recombinant Protein is a single, non-glycosylated polypeptide chain that contains 125 amino acids and has a molecular mass of 14 kDa. The IL-4 is purified by proprietary chromatographic techniques.
Summary
IL-4 is a cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning. Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is also expressed on nonhematopoietic cells. Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6.
Other Names
Interleukin 4, IL4, B-cell stimulatory factor 1 (BSF-1), Lymphocyte stimulatory factor 1, UniProtKB# P20096.
Source
Expressed in E. coli.
Formulation
Sterile filtered white lyophilized (freeze-dried) powder. IL-4 protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS pH 7.4 and 5% trehalose.
Amino Acid Sequence
HGCNDSPLR EIINTLNQVT EKGTPCTEMF VPDVLTATRN TTENELICRA SRVLRKFYFP RDVPPCLKNK SGVLGELRKL CRGVSGLNSL RSCTVNESTL TTLKDFLESL KSILRGKYLQ SCTSMS.
Purity
Greater than 95.0% as determined by:
- Analysis by RP-HPLC.
- Analysis by SDS-PAGE.
Protein Content
Protein quantitation was carried out by two independent methods:
- UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
- Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.
Biological Activity
The ED50 as determined in a cell proliferation assay using rat splenocytes is less than 2 ng/ml corresponding to a specific activity of 500,000 IU/mg.
Reconstitution
It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized IL-4, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, IL-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.