Description
ISOkine® Epidermal Growth Factor (EGF) Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 53 amino acids and an N-terminal 6 a.a. histidine tag for a total length of 59 a.a. and has a predicted molecular mass of 7 kDa. The recombinant protein migrates with an apparent molecular mass of 9.5 kDa in SDS PAGE.
ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.
Summary
Epidermal Growth Factor (EGF) is a potent growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation. It stimulates the proliferation of various epidermal and epithelial cells. EGF has been shown to inhibit gastric secretion, promote colony formation of epidermal cells in culture, and be involved in wound healing. EGF is the prototype of a large family of EGF-like proteins, it signals through the c-erbB receptor. Recombinant human EGF is a globular protein with 3 intramolecular disulfide bonds essential for biological activity. Approximately 70% homology is found between human EGF and EGF isolated from other species. The relative positions of the cysteine residues are conserved.
Source
Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.
Manufacturer: ORF Genetics
Country of Origin: Iceland
Formulation
2xPBS pH 7.2, sterile filtered through 0.22 µm filter.
Purity
Greater than 95% by SDS-PAGE gel analysis
Reconstitution
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).
Stability
The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Biological Activity
The bioactivity of ISOkine® recombinant human EGF is assayed by measuring its dose-dependent effect on the proliferation of 3T3 cells. The ED50 for this effect using EGF is typically < 0.1 ng/ml, corresponding to a specific activity > 10 x10e6 U/mg. We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source.
Endotoxin Level
Endotoxin level is < 0.01 EU per μg of ISOkine® product (< 0.01 EU/μg) by LAL method.
Pyrogen Free
Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Documents
Product References
- Greggi, Chiara et al. “Modulation of Carnitine Palmitoyl Transferase 1b Expression and Activity in Muscle Pathophysiology in Osteoarthritis and Osteoporosis.” Biomolecules vol. 14,10 1289. 12 Oct. 2024, doi:10.3390/biom14101289
- Bodiou, Vincent et al. “Attachment promoting compounds significantly enhance cell proliferation and purity of bovine satellite cells grown on microcarriers in the absence of serum.” Frontiers in bioengineering and biotechnology vol. 12 1443914. 1 Nov. 2024, doi:10.3389/fbioe.2024.1443914
- Pekec, Tina et al. “Ferritin-mediated iron detoxification promotes hypothermia survival in Caenorhabditis elegans and murine neurons.” Nature communications vol. 13,1 4883. 19 Aug. 2022, doi:10.1038/s41467-022-32500-z
- D'Agostino, Stefania et al. “Rhabdomyosarcoma Cells Produce Their Own Extracellular Matrix With Minimal Involvement of Cancer-Associated Fibroblasts: A Preliminary Study.” Frontiers in oncology vol. 10 600980. 29 Jan. 2021, doi:10.3389/fonc.2020.600980
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