Description
ISOkine® Interleukin-6 (IL-6) Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions has a molecular weight of 21.5 kDa and contains 185 amino acid residues.
ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.
Summary
IL-6 is a multifunctional cytokine with an essential role in host defense by regulating immune and inflammatory responses. It is expressed by various cell types, including T cells, B cells, monocytes/macrophages, fibroblasts, and possibly tumor cells. IL-6 has diverse biological functions, stimulating B-cell differentiation and antibody production, synergizing with other factors in hematopoiesis and platelet production, inducing hepatic acute-phase protein expression, and regulating bone metabolism.
Source
Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.
Manufacturer: ORF Genetics
Country of Origin: Iceland
Formulation
PBS pH 7.2, sterile filtered through 0.2 µm filter
Purity
Greater than 95% by SDS-PAGE gel analysis
Reconstitution
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).
Stability
The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Biological Activity
Each batch of ISOkine® growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.
Endotoxin Level
Endotoxin level is < 0.01 EU/μg of ISOkine® product by LAL method.
Pyrogen Free
Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Synonyms
Interleukin 6, IL6, B-cell stimulatory factor 2 (BSF-2), CTL differentiation factor (CDF), Hybridoma growth factor (HGF), Interferon beta-2 (IFN-beta-2), IFNB2, UniProtKB# P05231.
Product References
- Root, Sierra H, and Héctor L Aguila. “Novel population of human monocyte and osteoclast progenitors from pluripotent stem cells and peripheral blood.” Blood advances vol. 5,21 (2021): 4435-4446. doi:10.1182/bloodadvances.2021004552
- Cuomo, Francesca et al. “Pro-inflammatory cytokines activate hypoxia-inducible factor 3α via epigenetic changes in mesenchymal stromal/stem cells.” Scientific reports vol. 8,1 5842. 11 Apr. 2018, doi:10.1038/s41598-018-24221-5
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Ilex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors & cytokines manufactured by ORF Genetics Ltd.