Description
ISOkine® LIF Mouse, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 180 amino acids and an N-terminal 10 amino acid histidine tag for a total length of 190 amino acids and has a predicted molecular mass of 21.2 kDa.
The ISOkine® mLIF has been validated in standard mouse embryonic stem cell (mESC) based gene-targeting for generation of knock-out mice.
ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.
Summary
Leukemia inhibitory factor (LIF) is a pleiotropic lymphoid factor belonging to the interleukin-6 family of cytokines. It was initially described as a factor inhibiting the proliferation of myeloid leukemia cells and inducing their differentiation into macrophages. Other activities of LIF include inhibition of adipogenesis and roles in cholinergic neuron differentiation, hematopoiesis, inflammation and bone metabolism. LIF inhibits the differentiation of embryonic stem cells, it is therefore important in ES cell culture. Human and mouse LIF share 78% amino acid homology, the human version is equally active on human and mouse cells but murine LIF is not active on human cells.
Source
Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.
Manufacturer: ORF Genetics
Country of Origin: Iceland
Formulation
2xPBS pH 7.2, sterile filtered through 0.22 µm filter
Purity
> 95% by SDS-PAGE gel analysis.
Reconstitution
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).
Stability
The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Biological Activity
Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant mouse LIF is assayed by measuring its dose-dependent effect on the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect using LIF is typically <0.5 ng/ml. Optimal concentration should be determined for specific applications and cell lines.
Endotoxin Level
Endotoxin level is < 0.01 EU per μg of ISOkine® product by LAL method.
Pyrogen Free
Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Synonyms
Murine Leukemia inhibitory factor, Differentiation-stimulating factor (D factor), Melanoma-derived LPL inhibitor (MLPLI).
Documents
Product References
- Jiang, Zhisheng et al. “A conserved expression signature predicts growth rate and reveals cell & lineage-specific differences.” PLoS computational biology vol. 17,11 e1009582. 11 Nov. 2021, doi:10.1371/journal.pcbi.1009582
- Yano, Yoshiteru et al. “Non-neural and cardiac differentiating properties of Tbx6-expressing mouse embryonic stem cells.” Regenerative therapy vol. 3 1-6. 1 Mar. 2016, doi:10.1016/j.reth.2016.02.001
- Yoon, Jaewoong et al. “Generation of nanovesicles with sliced cellular membrane fragments for exogenous material delivery.” Biomaterials vol. 59 (2015): 12-20. doi:10.1016/j.biomaterials.2015.04.028
- Wilkinson, Adam C et al. “Single site-specific integration targeting coupled with embryonic stem cell differentiation provides a high-throughput alternative to in vivo enhancer analyses.” Biology open vol. 2,11 1229-38. 7 Oct. 2013, doi:10.1242/bio.20136296
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Ilex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors & cytokines manufactured by ORF Genetics Ltd.