Description
ISOkine® LR3 IGF-I Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions is a 9.1 kDa, single, non-glycosylated polypeptide chain containing 83 amino acid residues and a 16 a.a. Histidine-based tag for a total length of 99 a.a. The recombinant protein migrates with an apparent molecular mass of 12.5 kDa in SDS-PAGE.
ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.
Summary
Insulin-like growth factor 1 (IGF-1) is a polypeptide growth factor that promotes cell proliferation and survival in tissues such as muscle, bone, and cartilage. It is mainly produced by the liver, though many tissues express it at specific times. IGF-1 is part of the insulin gene family and is structurally similar to insulin but has greater growth promoting activity. IGF-1 LR3 is a recombinant analog of human IGF-1 comprised of the complete IGF-1 sequence, with an Arginine substitution for the third position Glutamic acid, and a 13 amino acid length N terminus peptide extension. Specifically engineered for higher biological potency in vitro, IGF-1 LR3 has an increased half-life and a binding aversion to native proteins within the body that make it ideal for both research and large-scale culturing.
Source
Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.
Manufacturer: ORF Genetics
Country of Origin: Iceland
Formulation
2xPBS pH 7.2, sterile filtered through 0.22 µm filter
Purity
Greater than 95% by SDS-PAGE gel analysis
Reconstitution
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).
Stability
The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Biological Activity
The bioactivity of ISOkine® LR3 IGF-I Human is assayed by measuring its dose dependent effect on proliferation of FDC-P1 cells. The ED50 for this effect using IGF-1, LR3 is < 2 ng/ml, corresponding to a specific activity >0.5 10 x10e6 U/mg. We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine® growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.
Endotoxin Level
Endotoxin level is < 0.01 EU/μg of ISOkine® product by LAL method.
Pyrogen Free
Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Synonyms
Long R3 IGF-I, LR3 Insulin-like growth factor I, Insulin-like growth factor 1, IGF-I LR3, IGFI, IGF-1, IGF1, Mechano growth factor (MGF), Somatomedin-C, IBP1.