Description
ISOkine® PDGF-BB Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 109 amino acids and an N-terminal 6 amino acid histidine tag for a total length of 115 amino acids and has a predicted molecular mass of 13.1 kDa
ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.
Summary
Platelet-Derived Growth Factor-BB (PDGF-BB) is a disulfide-linked homodimer of two B-chains (109 aa each). It is stored in platelet α-granules and is released following activation of platelets. PDGF-BB has potent mitogenic effects on various cell types such as cells of connective tissue, bone and cartilage, and smooth muscle. It promotes bone formation and soft tissue repair, stimulating wound healing processes. PDGF-BB is involved in the development of the nervous system and may act as a neuronal regulatory agent via nerve regeneration and proliferation. It also plays a role in the epithelial cell development of the respiratory system, cell migration, and angiogenesis. PDGF-BB is a key component to add in many chemically defined and other serum-free media formulations
Source
Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.
Manufacturer: ORF Genetics
Country of Origin: Iceland
Formulation
2xPBS pH 7.2, sterile filtered through 0.22 µm filter
Purity
≥ 95% by SDS-PAGE gel analysis.
Reconstitution
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).
Stability
The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Biological Activity
Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant human PDGF-BB is assayed by measuring its dose-dependent effect on the proliferation of 3T3 cells. The ED50 for this effect using PDGF-BB is typically <10 ng/ml. Optimal concentration should be determined for specific applications and cell lines.
Endotoxin Level
Endotoxin level is < 0.01 EU per μg of ISOkine® product by LAL method.
Pyrogen Free
Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.
Usage
This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Synonyms
PDGF BB, PDGFBB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis.
Documents
Related Products
Ilex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors & cytokines manufactured by ORF Genetics Ltd.