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Lysostaphin, E. coli Recombinant Protein

A426901
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$99.00
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Regular price
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Description

Lysostaphin Recombinant produced in E. coli is a non-glycosylated polypeptide chain with a molecular mass of 26.92 kDa.

Summary

Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Bacteriolytic  Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination. 

Type

Recombinant protein

CAS Registry No.

9011-93-2

Purity

97.5%

Biological Activity

Assessed by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH-8, 30°C. Lysostaphin is a zinc enzyme therefore EDTA is an inhibitory factor. Please refer to Certificate of Analysis for specific activity of each lot number.

Formulation

Sterile filtered lyophilized powder. The protein was lyophilized without any additives.

Reconstitution

It is recommended to reconstitute the lyophilized Lysostaphin in 20mM sodium acetate, pH 4.5 for optimal stability, which can then be further diluted to other aqueous solutions such as 200mM Tris HCL pH-8 for optimal activity. 

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Lyophilized Lysostaphin although stable at room temperature for 2 weeks, should be stored desiccated below -18°C. Upon reconstitution Lysostaphin can be stored at 4°C up to 3 weeks and for future use below -18°C. Please prevent freeze-thaw cycles.

Usage

This product is intended to Laboratory Research Use Only.  This product may not be used a drug, agricultural product, food additives.

Other names

EC 3.4.24.75, Glycyl-glycine endopeptidase, Staphylococcus simulans metalloendopeptidase, Staphylococcus aureus bacteriocin (antimicrobial)

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