Description
PAN-Biotech Dispase II (Neutral Protease, Grade II) from Bacillus polymyxa in DPBS, w/o: Ca, Mg, 2.4 U/ml.
Dispase (purified neutral protease) is a non-mammalian, animal origin-free, neutral metalloprotease produced by Bacillus polymyxa. Dispase hydrolyses N-terminal peptide bonds of non-polar amino acid residues and is classified as an aminoendopeptidase. Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) in cell culture since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagenase.
Application
Dispase II is commonly used to separate skin epidermis from dermis (leaving intact epithelial sheets), and stem cell, hepatocyte, and other cell isolation applications. However, due to the diversity of variables involved, exact isolation conditions should be determined empirically for each cell/tissue application.
Specifications
Cat. No. P10-032100
| Ca/Mg Content: | Without Ca and Mg |
|
Concentration: |
1x (2.4 U/ml) |
|
Liquid/Powder: |
Liquid |
| Phenol Red: | Without phenol red |
| Product Category: | Enzymes for cell dissociation |
| Product Type: | Dispase |
| Size: | 100 ml |
| Sterile: | Yes |
| Storage Temperature: | -20°C |
| In Solution: | In DPBS |
| Activators: | Divalent cations including Ca2+, Mg2+, Mn2+, and Fe2+. |
| Inhibitors: | EDTA, EGTA, 1-10-phenanthroline and heavy metals. |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Resources
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Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.