NOTE: This item has been discontinued. Please consider using one of our Serum Substitutes as a better alternative.
Description
Highly qualified fetal bovine serum (FBS) of US origin, 0.1 μm sterile filtered.
- Example Certificate of Analysis (CoA) can be found here.
Summary
Fetal Bovine Serum (FBS), also known as Fetal Calf Serum (FCS), is the most commonly used serum to supplement cell culture media due to its high content of growth factors. FBS is effective in promoting and sustaining growth of vertebrate, mammalian and insect cells.
Specifications
| Country of Origin | United States |
| Endotoxin Content | Refer to CoA |
| Filter Pore Size | 0.1 μm sterile filtered |
| Liquid/Powder | Liquid |
| Product Category | Media supplements |
| Product Type | FBS |
| Size | Various |
| Species of Origin | Bovine |
| Sterile | Yes |
| Storage Temperature | -20°C |
Advantages
Each batch of FBS Standard is subject to rigorous testing for quality control and characterization:
| Test | Method |
| pH value |
Measured with pH-electrode |
|
Osmolality |
Analyzed by freezing point depression |
| Hemoglobin |
Determined spectrophotometrically at three different wave lengths |
| Endotoxin (LPS) |
Kinetic limulus amoebocyte lysate test (LAL) |
| Total Protein |
Colorimetric test (Biuret reaction) |
| Albumin, globulins |
Serum protein electrophoresis (SPEP) |
| IgG | ELISA |
| Glucose | Colorimetric test |
| Cholesterol | Colorimetric test |
| Triglycerides | Colorimetric test |
| Sterility | The absence of bacterial or fungal contamination is verified by dual incubation with Caso-Bouillon or Thioglycolat- Bouillon according to Ph. Eur. at 32°C and 20 °C |
| Mycoplasma | qPCR of microbial cultures |
| Cell growth | Growth test of specific cells |
Thorough sterility, mycoplasma, and virus testing:
| Test | Specification | Result |
| Sterility (incubation at 32°C) | sterile | sterile |
|
Sterility (incubation at 20°C) |
sterile | sterile |
| Sterility (mycoplasma) |
not detected |
not detected |
| Bovine viral diarrhoea virus (BVDV) | negative | negative |
| Bovine herpes virus (BHV-1) | negative | negative |
| Para-influenza virus type 3 (PI-3) | negative | negative |
| Bluetongue virus (BTV) | negative | negative |
| Bovine adenovirus (BAV) | negative | negative |
| Bovine parvovirus (BPV) | negative | negative |
| Bovine respiratory syncytial virus (BRSV) | negative | negative |
| Rabies virus | negative | negative |
| Reovirus (RV-3) | negative | negative |
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
- Certificate of Analysis (CoA) (example)
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Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech GmbH.