Introduction
Minimum Essential Medium (MEM), developed by Harry Eagle, is a widely used synthetic cell culture media. After finding that Eagle's Basal Medium (BME) did not meet specific nutritional requirements of normal mammalian fibroblasts and certain HeLa cells, MEM was created with higher concentrations of amino acids to more closely resemble the protein composition of mammalian cells. When supplemented with serum, MEM is suitable for the cultivation of a wide variety of cells grown in monolayers. MEM often serves as a base medium for many further modifications.
MEM-NEAA: The addition of non-essential amino acids (NEAA) to MEM (MEM-NEAA) has increased the suitability of this medium for a broad range of applications.
A GMP Grade version is now available: PAN-Biotech MEM Eagle w: EBSS, w: 2 mM L-Glutamine, w: 1 mM Sodium pyruvate, w: NEAA, w: 1.5 g/L NaHCO3, GMP Grade.
Specifications
Cat. No. P04-08056: Contains Earle’s Balanced Salt Solution (EBSS) and non-essential amino acids (NEAA).
| Quality: | Research Use Only (RUO) |
|---|---|
| Bicarbonate: | With 1.5 g/L Sodium Bicarbonate |
| Endotoxin Content: | Endotoxin Content unspecified |
| Glucose: | With glucose |
| Glutamine: | With 2 mM L-Glutamine |
| HEPES: | Without HEPES |
| Liquid / Powder: | Liquid |
| Phenol Red: | With Phenol Red |
| Product Category: | Cell Culture Media |
| Product Type: | MEM |
| Size: | 500 ml bottles |
| Sodium Pyruvate: | With 1 mM Sodium Pyruvate |
| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Documents
Product References
- Palmieri, Fabio et al. “One-Step Soft Agar Enrichment and Isolation of Human Lung Bacteria Inhibiting the Germination of Aspergillus fumigatus Conidia.” Microorganisms vol. 12,10 2025. 7 Oct. 2024, doi:10.3390/microorganisms12102025
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