Description
Recombinant Human Insulin Powder for cell culture applications.
Recombinant Human Insulin is a two chain, non-glycosylated polypeptide chain that contains 51 amino acids and has a molecular mass of ~5808 Dalton. The protein is purified by proprietary chromatographic techniques
Summary
Insulin is a peptide hormone produced by beta cells of the pancreatic islets encoded in humans by the INS gene. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver (UniProtKb_P01308). Recombinant Human Insulin is a commonly used media supplement in mammalian cell culture.
Specifications
| CAS Registry No. | 11061-68-0 |
|
Liquid/Powder |
Powder |
|
Product Category |
Media supplements |
|
Product Type |
Insulin |
|
Size |
25 mg, 100 mg, 250 mg, 1 g, other sizes available on request. |
|
Sterile |
Yes |
| Biological Activity: | ≥ 27.5 units/mg |
| Storage Temperature | -20°C |
| Manufacturer | PAN-Biotech GmbH |
| Country of Origin | Germany |
Reconstitution
It is recommended to reconstitute the lyophilized Insulin in sterile 0.005N HCl not more than 1 mg/ml.
Storage
Lyophilized Insulin, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Insulin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
Product References
- Bodiou, Vincent et al. “Attachment promoting compounds significantly enhance cell proliferation and purity of bovine satellite cells grown on microcarriers in the absence of serum.” Frontiers in bioengineering and biotechnology vol. 12 1443914. 1 Nov. 2024, doi:10.3389/fbioe.2024.1443914
- Maus, Deborah et al. “Generation of Mature Toxoplasma gondii Bradyzoites in Human Immortalized Myogenic KD3 Cells.” Bio-protocol vol. 14,1 e4916. 5 Jan. 2024, doi:10.21769/BioProtoc.4916
- Caponi, Joseph J et al. “Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets.” STAR protocols, vol. 4,2 102143. 9 Mar. 2023, doi:10.1016/j.xpro.2023.102143
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Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech GmbH.