Description
PAN-Biotech Trypsin Inhibitor Solution 1 mg/ml is a sterile solution of plant-based inhibitors against trypsin and in some effect to chymotrypsin and plasmin. The trypsin neutralizing solution does not inhibit metalloproteases, tissue based kallikrein, and acid or thioproteases.
Composition
Trypsin Inhibitor Solution contains natural plant-based protease inhibitor proteins. The biological activity is depending on trypsin concentration.
Suitability
For inhibiting trypsin activity in serum-free culture of adherent cells.
Trypsin is used for dissociation of adhesion-dependent cells. Serum contains natural trypsin inhibitors. In serum-free cell culture the neutralizing effect of serum is absent. Therefore, it is crucial to completely block proteolytic activity from trypsin after detachment of the cells.
Inhibits trypsin activity; chymotrypsin and other proteolytic enzymes are inhibited to a lesser extent.
Advantage
Trypsin Inhibitor Solution contains protease inhibitors as well as growth promoting agents. After detachment of the cells and re-suspension in serum-free medium, Trypsin Inhibitor Solution supports the recovery, attachment, and spreading of the cells. The usual centrifugation step to remove trypsin inhibitor from the cells should be omitted with this product. This time-saving feature facilitates a more convenient and efficient serum-free cell culture. Please follow instructions for use closely to take advantage of this unique feature.
Specifications
Cat. No. P10-033100
|
Liquid/Powder: |
Liquid |
|
Product Category: |
Enzymes for cell dissociation |
|
Product Type: |
Trypsin inhibitor |
| Size: | 100 ml bottles |
|
Sterile: |
Yes |
| Storage Temperature: | -20°C |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin: | Germany |
Application Note
Cell exposure to trypsin solution should be as brief as possible. Overexposure to trypsin can damage cells. Cell cultures under serum-free conditions in general detach more readily and are more sensitive to trypsin. Failure to neutralize trypsin may result in a loss of the culture.
Storage conditions & stability
Stability: 2 years from date of production
NOTE: Repeated freeze and thaw cycles will reduce activity and should be avoided. The product is stable for up to 2 years at recommended storage conditions. Thawed Trypsin Inhibitor can be stored short term at 2-8°C.
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Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.