PromedeusLab GDF-15 ELISA, Human

Description

Enzyme immunoassay for the quantitative determination of growth differentiation factor 15 (GDF-15) in human serum and plasma samples. Quality controls are included with the kit. Developed and manufactured in Europe by PromedeusLab.

Summary

Growth differentiation factor 15 (GDF15) is a member of the TGFβ superfamily whose expression is increased in response to cellular stress and disease as well as by metformin (1). Identified as a new heart-derived endocrine hormone that regulates body growth, GDF15 has a local cardioprotective role, presumably due to its autocrine/paracrine properties: antioxidative, anti-inflammatory, antiapoptotic. GDF15 expression is highly induced in cardiomyocytes after ischemia/reperfusion and in the heart within hours after myocardial infarction (MI). GDF15 may be a predictive biomarker of adverse cardiac events (2).

Available evidence also suggests that a substantial amount of GDF15 is secreted in various human cancers, such as ovarian cancer, prostate cancer, and breast cancer, among others (3).

Other Names

Growth/differentiation factor 15, Macrophage inhibitory cytokine 1 (MIC-1, MIC1), NSAID-activated gene 1 protein (NAG-1), NSAID-regulated gene 1 protein (NRG-1), Placental TGF-beta (PTGFB), Placental bone morphogenetic protein, Placental BMP, Prostate differentiation factor, UniProtKB# Q99988.

Principle of Method

The microtiter plate is coated with the antibody specifically binding the Growth differentiation factor 15. The human serum or plasma is incubated in the plate with the capture antibody.

The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4).

The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of GDF15 in the specimen. The concentration of GDF15 in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.

Sample Types

Serum, Plasma

Typical Standard Curve

Assay Range: 25 pg/mL - 800 pg/m

Sensitivity

The limit of detection, defined as a concentration of human GDF-15 giving absorbance higher than absorbance of blank + 3 standard deviations, is better than 6.25 p/mL of sample.

Precision

Intra-Assay

Inter-Assay (Run-to-Run)

Accuracy

Dilution Linearity:

Spiking Recovery:

Storage

The kit must be stored at 2–8 °C. The opened components can be stored for one week at 2–8 °C.

Usage

This assay is for in vitro research use only and is not for use in diagnostic procedures.

Resources

• Product Datasheet (PDS)

Summary References

1. Wang D, Day EA, Townsend LK, Djordjevic D, Jørgensen SB, Steinberg GR. GDF15: emerging biology and therapeutic applications for obesity and cardiometabolic disease. Nat Rev Endocrinol. 2021 Oct;17(10):592-607. doi: 10.1038/s41574-021-00529-7. Epub 2021 Aug 11. PMID: 34381196.
2. Rochette L, Dogon G, Zeller M, Cottin Y, Vergely C. GDF15 and Cardiac Cells: Current Concepts and New Insights. Int J Mol Sci. 2021 Aug 18;22(16):8889. doi: 10.3390/ijms22168889. PMID: 34445593; PMCID: PMC8396208.
3. Li S, Ma YM, Zheng PS, Zhang P. GDF15 promotes the proliferation of cervical cancer cells by phosphorylating AKT1 and Erk1/2 through the receptor ErbB2. J Exp Clin Cancer Res. 2018 Apr 10;37(1):80. doi: 10.1186/s13046-018-0744-0. PMID: 29636108; PMCID: PMC5894198.

 Ilex Life Sciences LLC is an authorized distributor of PromedeusLab.