Description
Enzyme Immunoassay kit for the quantitative determination Kidney Injury Molecule 1 (KIM-1) in human urine and serum samples. Quality controls are included with the kit. Developed and manufactured in Europe by PromedeusLab.
Summary
The urinary kidney injury molecule 1 (KIM-1) participates in renal tissue damage and repair and is proposed as a biomarker of early and subclinical acute kidney injury (AKI). KIM-1 is also elevated in the urine of a significant fraction of patients apparently recovered from an AKI. Besides its potential utility in the early and subclinical diagnosis of renal damage, research suggests a new application of urinary KIM-1 in the non-invasive follow-up of renal repair after AKI (1).
Other Names
Kidney injury molecule 1 (KIM-1, KIM1), T-cell immunoglobulin and mucin domain-containing protein 1 (TIMD-1, TIMD1), T-cell immunoglobulin mucin receptor 1 (TIM, TIM-1, TIM1), Hepatitis A virus cellular receptor 1 (HAVcr-1, HAVCR, HAVCR1), T-cell membrane protein 1, CD365, UniProtKB# Q96D42.
Principle of Method
The microtiter plate is coated with the antibody specifically binding the Kidney Injury Molecule 1. The human serum or plasma is incubated in the plate with the capture antibody.
The specimen is washed out and the specifically bound protein is incubated with HRP-labelled detection antibody. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4).
The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of KIM-1 in the specimen. The concentration of KIM-1 in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values
Sample Types
Urine, Serum
Typical Standard Curve
Assay Range: 15.625 pg/mL - 1000 pg/mL
Sensitivity
The limit of detection, defined as a concentration of human KIM-1 giving absorbance higher than absorbance of blank + 3 standard deviations, is better than 45 pg/mL of sample.
Precision
Intra-Assay
| Test sample | Mean (pg/mL) | SD | CV |
| 1 |
519 |
8.8 |
1.7% |
| 2 |
287 |
15.6 |
5.4% |
Inter-Assay (Run-to-Run)
| Test sample | Mean (pg/mL) | SD | CV |
| 1 |
284 |
16 |
5.7% |
| 2 |
114 |
15 |
13.3% |
Accuracy
Dilution Linearity:
| Test sample | Dilution |
Measured Concentration (pg/mL) |
Expected Concentration (pg/mL) |
Yield |
| 1 |
509 |
- | - | |
| 1 | 2x | 230 |
254 |
90% |
| 1 | 4x | 106 |
127 |
83% |
| 1 | 8x | 52 |
64 |
82% |
| 2 |
448 |
- |
- | |
| 2 | 2x | 196 |
224 |
87% |
| 2 | 4x | 99 |
112 |
89% |
| 2 | 8x | 54 |
56 |
96% |
Spiking Recovery:
| Test sample | Spike (ng/mL) |
Measured Concentration (pg/mL) |
Expected Concentration (pg/mL) |
Yield |
| 1 |
- | 236 |
- | - |
| 1 | 360 |
663 |
596 |
111% |
| 1 | 180 |
446 |
416 |
107% |
| 1 | 90 |
341 |
326 |
105% |
Storage
The kit must be stored at 2–8 °C. The opened components can be stored for one week at 2–8 °C.
Usage
This assay is for in vitro research use only and is not for use in diagnostic procedures.
Resources
Summary Resources
- Cuesta C, Fuentes-Calvo I, Sancho-Martinez SM, Valentijn FA, Düwel A, Hidalgo-Thomas OA, Agüeros-Blanco C, Benito-Hernández A, Ramos-Barron MA, Gómez-Alamillo C, Arias M, Nguyen TQ, Goldschmeding R, Martínez-Salgado C, López-Hernández FJ. Urinary KIM-1 Correlates with the Subclinical Sequelae of Tubular Damage Persisting after the Apparent Functional Recovery from Intrinsic Acute Kidney Injury. Biomedicines. 2022 May 10;10(5):1106. doi: 10.3390/biomedicines10051106. PMID: 35625842; PMCID: PMC9139078.
Ilex Life Sciences LLC is an authorized distributor of PromedeusLab.