Spodopan, serum-free, protein-free medium for Insect-cells, w: L-Glutamine, w: 0.35 g/L NaHCO3, 500 ml (cat. no. P04-850500)
Spodopan, serum-free, protein-free medium for Insect-cells, w: L-Glutamine, w: 0.35 g/L NaHCO3, 1000 ml (cat. no. P04-851000)
Spodopan, serum-free, protein-free medium for Insect-cells, w: L-Glutamine, w/o: Pluronic, w: 0.35 g/L NaHCO3, 1000 ml (cat. no. P04-85051S)
  • Load image into Gallery viewer, Spodopan, serum-free, protein-free medium for Insect-cells, w: L-Glutamine, w: 0.35 g/L NaHCO3, 500 ml (cat. no. P04-850500)
  • Load image into Gallery viewer, Spodopan, serum-free, protein-free medium for Insect-cells, w: L-Glutamine, w: 0.35 g/L NaHCO3, 1000 ml (cat. no. P04-851000)
  • Load image into Gallery viewer, Spodopan, serum-free, protein-free medium for Insect-cells, w: L-Glutamine, w/o: Pluronic, w: 0.35 g/L NaHCO3, 1000 ml (cat. no. P04-85051S)

Spodopan: serum-free, protein-free medium for insect cells

P04-850500
Regular price
$59.00
Sale price
$59.00
Regular price
Available January 20th
Unit price
per 
Shipping calculated at checkout.

Description

PAN-Biotech Spodopan is a serum-free and protein-free medium for optimized growth of insect cells such as Sf9 and Sf21 (Spodoptera frugiperda) in suspension culture.

    Cell Type: Insect cells
    Culture Type: Suspension Culture
    Liquid / Powder: liquid
    Product Category: Serum-Free Media
    Size: Various
    Sterile: Yes

    Composition

    Spodopan, Protein-free medium for Insect-cells, w: L-Glutamine, w: 0.35 g/L NaHCO3: Spodopan contains amino acids, vitamins, salts, trace elements, lipids and growth promoting factors in a formulation optimized for insect cells. It contains no protein or any other components of human or animal origin, only traces of animal-derived components (< 0.2 % w/v) and hydrolysates (< 0.7 % w/v).

    Spodopan, Protein-free medium for Insect-cells, w: L-Glutamine, w/o: Pluronic, w: 0.35 g/L NaHCO3: Spodopan contains amino acids, vitamins, salts, trace elements, lipids and growth promoting factors in a formulation optimized for insect cells. It contains no protein or any other components of human or animal origin, only traces of animal-derived components (< 0.2 % w/v) and hydrolysates (< 0.7 % w/v).

    Suitability

    Spodopan is suitable for the cultivation of insect cells and the production of recombinant proteins and viruses (Baculovirus expression vector system, BEVS).

    Sf9 Cells in Spodopan culture media

    Figure 1. Sf9 insect cells in Spodopan media.

      Storage conditions & stability

      Storage: at +2°C - +8°C in the dark.

      Stability: 1 year from date of production

      Documents

      Product References

      • Dos Santos, Ália et al. “Binding partners regulate unfolding of myosin VI to activate the molecular motor.” The Biochemical journal vol. 479,13 (2022): 1409-1428. doi:10.1042/BCJ20220025
      • Hari-Gupta, Yukti et al. “Myosin VI regulates the spatial organisation of mammalian transcription initiation.” Nature communications vol. 13,1 1346. 15 Mar. 2022, doi:10.1038/s41467-022-28962-w
      • Kornakov, Nikolay et al. “The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.” The Journal of cell biology vol. 219,12 (2020): e202003072. doi:10.1083/jcb.202003072
      • Koch, Vasco et al. “Evidence for Stabilizing Selection Driving Mutational Turnover of Short Motifs in the Eukaryotic Complementary Sex Determiner (Csd) Protein.” G3 (Bethesda, Md.) vol. 8,12 3803-3812. 10 Dec. 2018, doi:10.1534/g3.118.200527
      • Mietzsch, Mario et al. “OneBac 2.0: Sf9 Cell Lines for Production of AAV1, AAV2, and AAV8 Vectors with Minimal Encapsidation of Foreign DNA.” Human gene therapy methods vol. 28,1 (2017): 15-22. doi:10.1089/hgtb.2016.164
      • Mietzsch, Mario et al. “Differential adeno-associated virus serotype-specific interaction patterns with synthetic heparins and other glycans.” Journal of virology vol. 88,5 (2014): 2991-3003. doi:10.1128/JVI.03371-13
      • Mietzsch, Mario et al. “OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy.” Human gene therapy vol. 25,3 (2014): 212-22. doi:10.1089/hum.2013.184
      • Weber, Michaela et al. “Incoming RNA virus nucleocapsids containing a 5'-triphosphorylated genome activate RIG-I and antiviral signaling.” Cell host & microbe vol. 13,3 (2013): 336-46. doi:10.1016/j.chom.2013.01.012

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