Description
PAN-Biotech Spodopan is a serum-free and protein-free cell culture medium for optimized growth of insect cells such as Sf9 and Sf21 (Spodoptera frugiperda) in suspension culture.
Specifications
Cat. No. P04-850500, P04-851000, P04-85051S
| Cell Type: | Insect cells |
|---|---|
| Culture Type: | Suspension Culture |
| Liquid / Powder: | Liquid |
| Product Category: | Serum-Free Media |
| Size: | Various |
| Sterile: | Yes |
| Storage Temperature: | +2°C - +8°C in the dark |
| Manufacturer: | PAN-Biotech GmbH |
| Country of Origin | Germany |
Suitability
Spodopan is suitable for the cultivation of insect cells and for the production of recombinant proteins and viruses using Baculovirus Expression Vector System (BEVS).
Figure 1. Sf9 insect cells in Spodopan media.
Composition
Spodopan, Protein-free medium for Insect-cells, w: L-Glutamine, w: 0.35 g/L NaHCO3: Spodopan contains amino acids, vitamins, salts, trace elements, lipids and growth promoting factors in a formulation optimized for insect cells. It contains no protein or any other components of human or animal origin, only traces of animal-derived components (< 0.2% w/v) and hydrolysates (< 0.7% w/v).
Documents
Product References
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- Hari-Gupta, Yukti et al. “Myosin VI regulates the spatial organisation of mammalian transcription initiation.” Nature communications vol. 13,1 1346. 15 Mar. 2022, doi:10.1038/s41467-022-28962-w
- Kornakov, Nikolay et al. “The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.” The Journal of cell biology vol. 219,12 (2020): e202003072. doi:10.1083/jcb.202003072
- Koch, Vasco et al. “Evidence for Stabilizing Selection Driving Mutational Turnover of Short Motifs in the Eukaryotic Complementary Sex Determiner (Csd) Protein.” G3 (Bethesda, Md.) vol. 8,12 3803-3812. 10 Dec. 2018, doi:10.1534/g3.118.200527
- Mietzsch, Mario et al. “OneBac 2.0: Sf9 Cell Lines for Production of AAV1, AAV2, and AAV8 Vectors with Minimal Encapsidation of Foreign DNA.” Human gene therapy methods vol. 28,1 (2017): 15-22. doi:10.1089/hgtb.2016.164
- Mietzsch, Mario et al. “Differential adeno-associated virus serotype-specific interaction patterns with synthetic heparins and other glycans.” Journal of virology vol. 88,5 (2014): 2991-3003. doi:10.1128/JVI.03371-13
- Mietzsch, Mario et al. “OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy.” Human gene therapy vol. 25,3 (2014): 212-22. doi:10.1089/hum.2013.184
- Weber, Michaela et al. “Incoming RNA virus nucleocapsids containing a 5'-triphosphorylated genome activate RIG-I and antiviral signaling.” Cell host & microbe vol. 13,3 (2013): 336-46. doi:10.1016/j.chom.2013.01.012
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