Ilex Life Sciences Stem Cell Factor (SCF) Rat, E. coli Recombinant Protein

Stem Cell Factor (SCF) Rat, E. coli Recombinant Protein

A33230010
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Description

Our bioactive Stem Cell Factor (SCF) Rat, E. coli Recombinant Protein is a single, non-glycosylated polypeptide chain containing 164 amino acids (26-189) and has a molecular mass of 18.4 kDa. The SCF is purified by proprietary chromatographic techniques.

Summary

Stem cell factor (SCF), also known as KIT ligand, binds to the receptor-type protein-tyrosine kinase KIT (CD117). This pleiotropic cytokine plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. SCF exists in both a soluble and a transmembrane isoform. SCF is commonly used in cell cultures in vitro to promote expansion of primitive hematopoietic stem cells and multi-potent progenitor cells.

Other Names

Rat SCF, Rat KIT ligand, c-Kit ligand, KITLG, KL, KL-1, KITL, mast cell growth factor, MGF, Steel factor, SF

Source

E. coli

Formulation

Sterile filtered white lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) solution in water containing 0.02% NaHCO3.

Purity

Greater than 98.0% as determined by SEC-HPLC and SDS-PAGE analysis.

Biological Activity

The ED50 is determined by the dose-dependent stimulation of the proliferation of human TF-1 cells which is < 10 ng/ml, corresponding to a specific activity of 100,000units/mg.

Amino Acid Sequence

MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.

    Reconstitution

    It is recommended to reconstitute the lyophilized SCF in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

    Shipping

    At ambient temperature. Upon receipt, store the product at the temperature recommended below.

    Storage/Expiration

    Lyophilized SCF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, SCF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    Usage

    This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.

    Resources

    Want to learn more about SCF? We have compiled the links below which contain information that you may find interesting.