Description
TAF15 Human, E. coli Recombinant Protein is a single polypeptide chain containing 282 amino acids (148-406) with a molecular mass of 30 kDa. TAF15 is fused to a 23 amino acid His-tag at N-terminus and is purified by proprietary chromatographic techniques.
Summary
TAF15 is a member of the TET family of RNA-binding proteins. TAF15 plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
In a 2023 Nature article, “TAF15 amyloid filaments in frontotemporal lobar degeneration", Stephan Tetter et al. reported the presence of abundant TAF15 amyloid filaments with a characteristic fold in frontotemporal lobar degeneration (FTLD), thus establishing TAF15 as a member of the small group of proteins known to form neurodegenerative disease-associated amyloid filaments. This discovery opens the door for more attention to the role of TAF15 proteinopathy in neurodegenerative disease.
Other Names
TAF-15, TATA-binding protein-associated factor 2N, 68 kDa TATA-binding protein-associated factor (TAF(II)68; TAFII68), RNA-binding protein 56, RBP56, TAF2N, UniProtKB# Q92804.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSSYHSQRE NYSHHTQDDR RDVSRYGEDN RGYGGSQGGG RGRGGYDKDG RGPMTGSSGG DRGGFKNFGG HRDYGPRTDA DSESDNSDNN TIFVQGLGEG VSTDQVGEFF KQIGIIKTNK KTGKPMINLY TDKDTGKPKG EATVSFDDPP SAKAAIDWFD GKEFHGNIIK VSFATRRPEF MRGGGSGGGR RGRGGYRGRG GFQGRGGDPK SGDWVCPNPS CGNMNFARRN SCNQCNEPRP EDSRPSGGDF RGRGYGGERG YR.
Source
E. coli
Formulation
Sterile-filtered clear solution. The TAF15 solution (0.25 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 30% glycerol and 1 mM DTT.
Purity
Greater than 90% as determined by SDS-PAGE.
Shipping
Ships on regular ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Resources
Want to learn more about TAF15? We have compiled the links below which contain information that you may find interesting:
- UniProt Knowledgebase: UniProtKB - Q92804 · RBP56_HUMAN (TAF15)
- National Library of Medicine: Gene ID: 8148: TAF15 - TATA-box binding protein associated factor 15 [Homo sapiens (human)]
- Tetter, Stephan et al. “TAF15 amyloid filaments in frontotemporal lobar degeneration.” Nature, 10.1038/s41586-023-06801-2. 6 Dec. 2023, doi:10.1038/s41586-023-06801-2