Introduction
The Collagen Hybridizing Peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo. By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.
CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging. CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture, without the use of synthetic fluorogenic matrices or genetically modified cells. CHP can measure and localize mechanical injury to collagenous tissue at the molecular level. It also enables assessment of collagen denaturation in decellularized extracellular matrix. In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot.
Description
B-CHP is labeled with biotin for avidin/streptavidin-mediated detection. B-CHP offers flexible detection options, such as non-green fluorescence and HRP-enzyme methods, to avoid background and enhance signal.
Specificity
B-CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).
Applications
- Cell invasion - reveal collagen turnover: Gain direct evidence of protease mechanisms by imaging degraded collagen in complex 3D settings. This robust method bypasses synthetic hydrogels and genetic modification, overcoming key assay limitations. Such clarity is vital for understanding cancer metastasis and finding new therapies against invasive diseases. More on cell invasion...
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Histopathology - unlock new insights in tissue damage: CHPs precisely detect and quantify denatured collagen, a hallmark of disease. CHPs are applicable to nearly all tissue types and a wide range of medical areas. More on histopathology...
- Mechanical damage - quantifying structural collagen damage: CHPs precisely detect and quantify denatured collagen to detect molecular level subfailure damage to collagen in mechanically stretched tendon fascicles. More on mechanical damage...
- Collagen identification - detecting collagen in SDS-PAGE gels: CHPs enable facile, sensitive, and specific collagen detection in SDS-PAGE gels by its triple-helical hybridization with SDS/heat-denatured collagen during electrophoresis. This simple method avoids Western blot steps (e.g., membrane transfer, blocking), saving time, effort, and materials. More on collagen identification...
- Collagen quantification - fluorescent quantification of total collagen: Total collagen staining with CHPs is made possible with a step-by-step guide with an Image-J / FIJI imaging protocol. More on collagen quantification...
Application Note
B-CHP provides the flexibility for visualizing damaged collagen using any user-selected fluorophore (e.g., AlexaFluor dyes conjugated streptavidin), or a non-fluorescence method (e.g., through NeutrAvidin-HRP mediated DAB staining).
In addition, B-CHP and commercial streptavidin-conjugated gold nanoparticles can be used in combination to label unfolded collagen molecules under TEM: collagen fibers in an intact or mechanically overloaded rat tail tendon probed by B-CHP and streptavidin conjugated 10nm-sized gold nanoparticles (scale bar: 200 nm).
Specifications
| Synonyms: | B-CHP, collagen mimetic peptide (CMP) |
| Product Category: | Collagen analysis |
| Molecular Weight: | 2762.01 g/mol |
|
Purity: |
95% by HPLC |
| Conjugate: | Biotin, single biotin tag per peptide |
| Format: | Purified lyophilized powder |
| Storage: | -20 °C as powder, 4 °C after reconstitution in water |
| Manufacturer: |
3Helix, Inc. |
Features
- More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM.
- High affinity and unparalleled specificity to collagen with essentially no nonspecific binding.
- Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding.
- Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval.
- A non-antibody approach with no species restrictions against any co-staining antibody.
- Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning.
- Stable in solution under 4 °C, eliminating the need to aliquot for storage.
Resources
- CHP Frequently Asked Questions (FAQ)
- B-CHP Product References
- User Guide: Total Collagen Histological Staining with CHPs
- User Guide: Damaged Collagen Histological Staining with CHPs
- User Guide: SDS-PAGE/In-Gel Western with CHPs
- User Guide: In Vivo Imaging with CHPs
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Related Products
- Collagen and Hydroxyproline Assays
- Extracellular Matrix Assays
- Extracellular Matrix Molecules
- Promed Bioscience high-purity collagen biomaterials
Ilex Life Sciences LLC is an authorized distributor of 3Helix, Inc. products.