The Biocolor Sircol™ Soluble Collagen Assay is a quantitative assay for measurement of both acid-soluble and pepsin-soluble collagens.
The assay can assess the rate of newly synthesized collagen. New collagen is produced during periods of rapid growth and development. New collagen is also generated during inflammation, wound healing and tumor development.
The Sircol Assay can monitor collagen produced in situ, or during in-vitro cell culture. It can also measure in-vitro extracellular matrix formation.
This assay is not suitable for covalent cross-linked collagen. However, the Sircol Insoluble Collagen Assay (S2000) replaces hydroxyproline analysis. This allows the customer to measure insoluble collagen within one working day.
Dye-binding method using Sircol™ Dye Reagent, which contains Sirius Red dye in picric acid and has been formulated for specific binding to collagen under the conditions defined in the Sircol assay protocol.
Which Sircol Collagen Assay should I use for my application?
The sensitivity of the Sircol Soluble Collagen Assay is 1.0 µg collagen. Quantities as low as 1.0 µg of collagen can be recovered from a 1 ml volume if the Collagen Isolation & Concentration protocol is used (See Sircol assay manual for more detail).
Assay Run Time
Type of Collagen Measured
Acid-soluble and pepsin-soluble collagens.
This kit is suitable for all mammalian collagens, Types I to V.
Sircol Flow Chart
Should I choose bovine or rat collagen kit?
The Sircol kit containing bovine collagen is suitable for use with all mammalian cells. The rat kit is offered primarily for customers who have difficulty importing/receiving kit with bovine material.
In the links below you will find the Sircol™ Soluble Collagen Assay protocol, publications that reference the Sircol™ Soluble Collagen Assay, and the Sircol™ Soluble Collagen Assay MSDS.
Want to learn more about collagen? We have compiled the links below that contain information which you may find interesting.
- The Collagen Family
- Collagen In Wound Healing
- Collagen Assembly at the Cell Surface: Dogmas Revisited
- Natural Biomaterials and Their Use as Bioinks for Printing Tissues
- Additive Biomanufacturing with Collagen Inks
- Collagen Mimetic Peptides
- The role of collagen in cancer: from bench to bedside
- Collagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications
Ilex Life Sciences LLC is an official distributor of Biocolor products.