Biocolor Sircol Soluble Collagen Assay
SCONC Sircol™ Isolation-Concentration & Acid-Neutralizing Reagents. Sircol™ Soluble/Insoluble Collagen Assay kit components. Manufactured by Biocolor Ltd.
SWASH Sircol™ Acid-Salt Wash Reagent. Sircol™ Soluble/Insoluble Collagen Assay kit components. Manufactured by Biocolor Ltd.
S1005 Sircol™ Dye Reagent (contains Sirius Red in picric acid). Sircol™ Soluble/Insoluble Collagen Assay kit components. Manufactured by Biocolor Ltd.
Sircol™ Collagen Bovine Reference Standard
Sircol Soluble Collagen Economy Assay S5000
Biocolor Sircol Collagen Soluble/Insoluble Combo Pack S4000
  • Load image into Gallery viewer, Biocolor Sircol Soluble Collagen Assay
  • Load image into Gallery viewer, SCONC Sircol™ Isolation-Concentration & Acid-Neutralizing Reagents. Sircol™ Soluble/Insoluble Collagen Assay kit components. Manufactured by Biocolor Ltd.
  • Load image into Gallery viewer, SWASH Sircol™ Acid-Salt Wash Reagent. Sircol™ Soluble/Insoluble Collagen Assay kit components. Manufactured by Biocolor Ltd.
  • Load image into Gallery viewer, S1005 Sircol™ Dye Reagent (contains Sirius Red in picric acid). Sircol™ Soluble/Insoluble Collagen Assay kit components. Manufactured by Biocolor Ltd.
  • Load image into Gallery viewer, Sircol™ Collagen Bovine Reference Standard
  • Load image into Gallery viewer, Sircol Soluble Collagen Economy Assay S5000
  • Load image into Gallery viewer, Biocolor Sircol Collagen Soluble/Insoluble Combo Pack S4000

Biocolor Sircol™ Soluble Collagen Assay

S1000
Regular price
$585.00
Sale price
$585.00
Regular price
$660.00
Unavailable
Unit price
per 
Shipping calculated at checkout.

Description

The Biocolor Sircol™ Soluble Collagen Assay is a quantitative assay for measurement of both acid-soluble and pepsin-soluble collagens.

The assay can assess the rate of newly synthesized collagen.  New collagen is produced during periods of rapid growth and development.  New collagen is also generated during inflammation, wound healing and tumor development.

The Sircol Assay can monitor collagen produced in situ, or during in-vitro cell culture.   It can also measure in-vitro extracellular matrix formation.

This assay is not suitable for covalent cross-linked collagen. However, the Sircol Insoluble Collagen Assay (S2000) replaces hydroxyproline analysis. This allows the customer to measure insoluble collagen within one working day.

Type

Colorimetric Assay (556 nm)

Dye-binding method using Sircol™ Dye Reagent, which contains Sirius Red dye in picric acid and has been formulated for specific binding to collagen under the conditions defined in the Sircol assay protocol.

Biocolor Sircol Soluble Collagen Assay Sirius Red Dye

Specifications

Assay Range 0 - 150 µg/ml or 0 - 500 µg/ml (depending on setup)
Limit of Detection 10 µg/ml
Assay Run-Time 1.5 hours
Sensitivity The sensitivity of the Sircol Soluble Collagen Assay is 1.0 µg collagen. Quantities as low as 1.0 µg of collagen can be recovered from a 1 ml volume if the Collagen Isolation & Concentration protocol is used (See Sircol assay manual for more detail).
Type of Collagen Measured
  • Acid-soluble and pepsin-soluble collagens.
  • Suitable for all mammalian collagens, Types I to V.

Suitable Samples

Soluble* collagens from mammalian sources**:

  • In-vivo: tissues, cartilages, fluids.

  • In-vitro: 2D/3D culture extracellular matrices / conditioned media.

* Collagens must be in soluble form, depending on sample this will require prior salt/acid/acid-pepsin extraction.

** Non-mammalian collagens may result in a reduced limit of detection. We recommend use of a collagen standard matched to the non-mammalian species under assay.


Which Sircol Collagen Assay should I use for my application?

Chart to determine which Sircol™ Collagen Assay is best for your application.

Should I choose bovine or rat collagen kit?

The Sircol™ kit containing bovine collagen standard is suitable for use with all mammalian cells. The Sircol™ kit with rat collagen standard is offered primarily for customers who have difficulty importing/receiving kit with bovine material.

Sircol Flow Chart

Biocolor Sircol™ Soluble Collagen Assay Flow Chart to determine the assay protocol for your application.

Resources

Want to learn more about collagen? We have compiled the links below that contain information which you may find interesting.

Related Products

Ilex Life Sciences LLC is an official distributor of Biocolor products.