S1000 Sircol™ Soluble Collagen Assay Kit. Manufactured by Biocolor Ltd.
SCONC Sircol™ Isolation-Concentration & Acid-Neutralizing Reagents. Sircol™ Soluble/Insoluble Collagen Assay kit components. Manufactured by Biocolor Ltd.
SWASH Sircol™ Acid-Salt Wash Reagent. Sircol™ Soluble/Insoluble Collagen Assay kit components. Manufactured by Biocolor Ltd.
S1005 Sircol™ Dye Reagent (contains Sirius Red in picric acid). Sircol™ Soluble/Insoluble Collagen Assay kit components. Manufactured by Biocolor Ltd.
S4000 Sircol™ Soluble Collagen Assay (110 assays) and Sircol™ Insoluble Collagen Assay (100 assays) combination pack.
S5000 Sircol™ Soluble Collagen Assay - Large Economy Size Kit (440 assays)
Sircol™ Collagen Bovine Reference Standard
  • Load image into Gallery viewer, S1000 Sircol™ Soluble Collagen Assay Kit. Manufactured by Biocolor Ltd.
  • Load image into Gallery viewer, SCONC Sircol™ Isolation-Concentration & Acid-Neutralizing Reagents. Sircol™ Soluble/Insoluble Collagen Assay kit components. Manufactured by Biocolor Ltd.
  • Load image into Gallery viewer, SWASH Sircol™ Acid-Salt Wash Reagent. Sircol™ Soluble/Insoluble Collagen Assay kit components. Manufactured by Biocolor Ltd.
  • Load image into Gallery viewer, S1005 Sircol™ Dye Reagent (contains Sirius Red in picric acid). Sircol™ Soluble/Insoluble Collagen Assay kit components. Manufactured by Biocolor Ltd.
  • Load image into Gallery viewer, S4000 Sircol™ Soluble Collagen Assay (110 assays) and Sircol™ Insoluble Collagen Assay (100 assays) combination pack.
  • Load image into Gallery viewer, S5000 Sircol™ Soluble Collagen Assay - Large Economy Size Kit (440 assays)
  • Load image into Gallery viewer, Sircol™ Collagen Bovine Reference Standard

Biocolor Sircol™ Soluble Collagen Assay

S1000
Regular price
$575.00
Sale price
$575.00
Regular price
$648.00
Temporarily out of stock
Unit price
per 
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Description

The Biocolor Sircol™ Soluble Collagen Assay is a quantitative assay for measurement of both acid-soluble and pepsin-soluble collagens.

The assay can assess the rate of newly synthesized collagen.  New collagen is produced during periods of rapid growth and development.  New collagen is also generated during inflammation, wound healing and tumor development.

The Sircol Assay can monitor collagen produced in situ, or during in-vitro cell culture.   It can also measure in-vitro extracellular matrix formation.

This assay is not suitable for covalent cross-linked collagen.  However, the Sircol Insoluble Collagen Assay (S2000) replaces hydroxyproline analysis.  This allows the customer to measure insoluble collagen within one working day.

Type

Colorimetric Assay

Dye-binding method using Sircol™ Dye Reagent, which contains Sirius Red dye in picric acid and has been formulated for specific binding to collagen under the conditions defined in the Sircol assay protocol.

Which Sircol Collagen Assay should I use for my application?

Chart to determine which Sircol™ Collagen Assay is best for your application.

Sensitivity

The sensitivity of the Sircol Soluble Collagen Assay is 1.0 µg collagen. Quantities as low as 1.0 µg of collagen can be recovered from a 1 ml volume if the Collagen Isolation & Concentration protocol is used (See Sircol assay manual for more detail).

Assay Run Time

1.5 hours 

Type of Collagen Measured

Acid-soluble and pepsin-soluble collagens.

This kit is suitable for all mammalian collagens, Types I to V.

Sircol Flow Chart

Biocolor Sircol™ Soluble Collagen Assay Flow Chart to determine the assay protocol for your application.

Should I choose bovine or rat collagen kit?

The Sircol kit containing bovine collagen is suitable for use with all mammalian cells.  The rat kit is offered primarily for customers who have difficulty importing/receiving kit with bovine material.

Resources

In the links below you will find the Sircol™ Soluble Collagen Assay protocol, publications that reference the Sircol™ Soluble Collagen Assay, and the Sircol™ Soluble Collagen Assay MSDS.

Want to learn more about collagen? We have compiled the links below that contain information which you may find interesting.

Ilex Life Sciences LLC is an official distributor of Biocolor products.