Introduction
The Collagen Hybridizing Peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo. By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.
The in vivo CHP incorporates a specialized sequence which allows them to be directly injected into animals without having to pre-activate them with a heating step. Therefore, the in vivo CHPs will always be in their active form and will not self-hybridize once injected. The sCy7.5-CHP is a powerful in vivo tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging. CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.
Description
In Vivo sCy7.5-CHPs are labeled with sulfonated-cyanine 7.5 dye (sulfo-Cy7.5) for near-infrared fluorescence (NIRF) detection. This wavelength offers better tissue penetration while minimizing tissue autofluorescence.
Specificity
In Vivo sCy7.5-CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).
Applications
- In vivo imaging
- Small animal imaging
Application Note
In Vivo sCy7.5-CHP dose can be adjusted based on animal model or application. Current dosing is based on a mouse model. Please check the protocol for details
Specifications
| Synonyms: | In Vivo sCy7.5-CHP, collagen mimetic peptide (CMP) |
| Product Category: | Collagen analysis |
| Molecular Weight: | 3703.1 g/mol |
|
Purity: |
90% by HPLC |
| Conjugate: | sulfo-Cy7.5, single sulfonated-cyanine 7.5 dye per peptide |
| Excitation: | 778 nm |
| Emission: | 797 nm |
| Format: | Purified lyophilized powder, green color |
| Storage: | -20 °C as powder, 4 °C for a few days once reconstituted |
| Manufacturer: |
3Helix, Inc. |
Features
- New sequence designed for in vivo use - does not need any pre-activation step unlike CHPs for histology.
- Near-infrared imaging probe minimizes the chance of autofluorescence in the gut or certain tissues.
- Can be used for systemic or in situ injection.
- Signal lasts at target sites for days, allowing for imaging at multiple time points with a single dose.
- High affinity and unparalleled specificity to collagen turnover with essentially no nonspecific binding.
- Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding.
- Stable as powder form, stored at -20 °C.
Resources
- CHP Frequently Asked Questions (FAQ)
- In Vivo sCy7.5-CHP Product References
- User Guide: In Vivo Imaging with CHPs
Usage
This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.
Related Products
- Collagen and Hydroxyproline Assays
- Extracellular Matrix Assays
- Extracellular Matrix Molecules
- Promed Bioscience high-purity collagen biomaterials
Ilex Life Sciences LLC is an authorized distributor of 3Helix, Inc. products.