Description
The Biocolor Sircol™ INSOLUBLE Collagen Assay is a dye binding method for the analysis of insoluble collagen fibres.
The assay can be used to assess the rate of production of newly laid down collagen fibres during periods of rapid growth, development, tissue repair, remodeling and wound healing. Sources of material includes tissues, bone and calcified tissue.
- It replaces the conventional hydroxyproline analysis. No more working with concentrated acids or alkalis!
- The Sircol Insoluble Assay uses mild acid and temperature treatment for 2 to 3 hrs.
- The solubility conversion to denatured collagen and subsequent measurement can be completed within one working day.
- The ratio of soluble and insoluble collagens can be measured using the same Sircol Dye Reagent.
Type
Colorimetric Assay (556 nm)
Dye-binding method using Sircol™ Dye Reagent, which contains Sirius Red dye in picric acid and has been formulated for specific binding to collagen under the conditions defined in the Sircol assay protocol.
Specifications
| Assay Range | 0 - 1000 µg/ml |
| Limit of Detection | 100 µg/ml |
| Assay Run-Time | 4 hours (including conversion of native insoluble collagen to soluble denatured collagen) |
| Type of Collagen Measured | Covalent crosslinked insoluble collagen |
Application Notes
Insoluble collagens must be converted into soluble form prior to assay. Instructions and reagents are provided with the kit. Depending on the sample, this will require prior salt/acid/acid-pepsin extraction.
Non-mammalian collagens may result in a reduced limit of detection. We recommend use of an assay standard matched to the non-mammalian species under assay.
Which Sircol Collagen Assay should I use for my application?
Resources
Want to learn more about collagen? We have compiled the links below that contain information which you may find interesting.
- The Collagen Family
- Collagen In Wound Healing
- Collagen Assembly at the Cell Surface: Dogmas Revisited
- Natural Biomaterials and Their Use as Bioinks for Printing Tissues
- Additive Biomanufacturing with Collagen Inks
- Collagen Mimetic Peptides
- The role of collagen in cancer: from bench to bedside
- Collagen of Extracellular Matrix from Marine Invertebrates and Its Medical Applications
Related Products
- Biocolor Sircol™ Soluble Collagen Assay
- Collagen and Hydroxyproline Assays
- Extracellular Matrix Assays
- Extracellular Matrix Molecules
Ilex Life Sciences LLC is an official distributor of Biocolor products.