Biocolor Sircol Insoluble Collagen Assay
S1005 Sircol™ Dye Reagent (Contains Sirius Red), component of Sircol™ Collagen Assays
SWASH Sircol™ Acid-Salt Wash Reagent (Contains Sirius Red), component of Sircol™ Collagen Assays
S2000 Biocolor Sircol™ INSOLUBLE Collagen Assay Fragmentation Reagent
Sircol Insoluble Collagen Economy Assay S6000
Biocolor Sircol Collagen Soluble/Insoluble Combo Pack S4000
S2000 Biocolor Sircol™ INSOLUBLE Collagen Assay Reference Standard 1.0 mg/ml
  • Load image into Gallery viewer, Biocolor Sircol Insoluble Collagen Assay
  • Load image into Gallery viewer, S1005 Sircol™ Dye Reagent (Contains Sirius Red), component of Sircol™ Collagen Assays
  • Load image into Gallery viewer, SWASH Sircol™ Acid-Salt Wash Reagent (Contains Sirius Red), component of Sircol™ Collagen Assays
  • Load image into Gallery viewer, S2000 Biocolor Sircol™ INSOLUBLE Collagen Assay Fragmentation Reagent
  • Load image into Gallery viewer, Sircol Insoluble Collagen Economy Assay S6000
  • Load image into Gallery viewer, Biocolor Sircol Collagen Soluble/Insoluble Combo Pack S4000
  • Load image into Gallery viewer, S2000 Biocolor Sircol™ INSOLUBLE Collagen Assay Reference Standard 1.0 mg/ml

Biocolor Sircol™ Insoluble Collagen Assay

S2000
Regular price
$550.00
Sale price
$550.00
Regular price
$578.00
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Description

The Biocolor Sircol™ INSOLUBLE Collagen Assay is a dye binding method for the analysis of insoluble collagen fibres.

The assay can be used to assess the rate of production of newly laid down collagen fibres during periods of rapid growth, development, tissue repair, remodeling and wound healing. Sources of material includes tissues, bone and calcified tissue.

  • It replaces the conventional hydroxyproline analysis. No more working with concentrated acids or alkalis!
  • The Sircol Insoluble Assay uses mild acid and temperature treatment for 2 to 3 hrs.
  • The solubility conversion to denatured collagen and subsequent measurement can be completed within one working day.
  • The ratio of soluble and insoluble collagens can be measured using the same Sircol Dye Reagent.

Type

Colorimetric Assay (556 nm)

Dye-binding method using Sircol™ Dye Reagent, which contains Sirius Red dye in picric acid and has been formulated for specific binding to collagen under the conditions defined in the Sircol assay protocol.

Biocolor Sircol Insoluble Collagen Assay Sirius Red Dye

Specifications

Assay Range 0 - 1000 µg/ml
Limit of Detection 100 µg/ml
Assay Run-Time 4 hours (including conversion of native insoluble collagen to soluble denatured collagen)
Type of Collagen Measured Covalent crosslinked insoluble collagen

Application Notes

Insoluble collagens must be converted into soluble form prior to assay. Instructions and reagents are provided with the kit. Depending on the sample, this will require prior salt/acid/acid-pepsin extraction.

Non-mammalian collagens may result in a reduced limit of detection. We recommend use of an assay standard matched to the non-mammalian species under assay.‍

Which Sircol Collagen Assay should I use for my application?

Which Sircol Collagen Assay should I use for my application? flow chart

Resources

Want to learn more about collagen? We have compiled the links below that contain information which you may find interesting.

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Ilex Life Sciences LLC is an official distributor of Biocolor products.