Introduction
Gelacell™ is a unique, 3D microfibrous scaffold specifically engineered for advanced in vitro 3D cell culture and tissue engineering applications. Designed as a non-woven, highly porous scaffold, Gelacell™ offers exceptional biocompatibility and non-toxicity across a variety of cell types.
Gelacell™ nanofibrous scaffolds are available in a variety of materials and formats.
Tailored for the cultivation of complex systems like organoids, cell microenvironments, and tissue regeneration, Gelacell™ also makes a robust platform for drug screening and other cell culture investigations. The scaffold’s optimized design ensures thermal, chemical, and mechanical stability, while providing substantial swelling capabilities and porosity for efficient nutrient diffusion and cellular waste build up prevention.
HaloScreening
Gelatex Technologies introduces HaloScreening, a ready-to-use 96-well cell culture plate embedded with microfibrous 3D scaffolds, produced using HaloSpin patented technology!
The HaloScreening plate is specifically designed for high-throughput screening (HTS) in both industrial and research settings. The bottom of each well contains scaffolds made of high-quality poly-L-lactic acid (PLLA) with optimized fiber diameters for improved cell-cell and cell-matrix interactions. The well plate is sterilized and ready to use, seamlessly integrating into standard workflows and automated systems. Downstream analysis and visualization can be conducted directly in the plate used for cell culturing, streamlining workflows and enhancing efficiency in HTS. This integrated approach is crucial for accelerating processes and improving productivity in industrial settings.
The well plates are also customizable to meet specific customer requirements and are applicable in drug screening, toxicology, compound testing, in vitro disease modeling, basic cell biology, and various other laboratory applications.
Features
- Scaffold: Highly porous, 3D, mimics in vivo environment, free of any animal derived components.
- Material: Medical grade PLLA, non-degradable, welded in 96-well plate (black).
- Cell visualization: Fluorescence imaging directly from the plate, also supports phase contrast.
- Cell viability: Compatible with most 2D assays, direct scanning through well plate reader.
- Sterilized & ready-to-use: Gamma sterilized plate, sealed in package.
- Reproducible & Reliable: Consistent cell growth ensures reproducibility and reliability.
- Easy handling: Compatible with industry-standard automated handling.
- Coating: Scaffolds can be coated with extracellular matrix (ECM) proteins for customers with specific needs.
- Technical support: Protocols for cell seeding, assays, and imaging are available.
- Manufacturing & cost: Produced in Class 7 clean room facilities; cost-effective and more economical.
- Stability: shipped and stored at room temperature.
Figure 1: SEM image of the PLLA scaffold.
Specifications
|
Catalog No.: |
GC0805RN-WP96-A |
| Polymer/Material: |
Poly-L-lactide (PLLA), also known as Poly-L-lactic acid, pharmaceutical grade |
| Format: |
Scaffolds in plate wells (ideal for short to medium term 3D cell culture):
|
|
Sterilization: |
Gamma sterilized (25 kGy) |
| Storage: | Store at room temperature; protect from moisture and chemical vapors. |
| Packaging: | Packed in sterilization pouches of paper and blue transparent film. |
| Scaffold Color: | White |
| Plate Color: | Black |
| Plate Size: | 96 well |
| Fiber Orientation: | Randomly aligned in a 3D morphology |
| Mean Fiber Diameter: | 1.3 μm - 2.6 μm |
| Solubility: | Insoluble in water and ethanol. |
| Absorptivity in PBS (24 hours): | 600% - 1200% |
| Degradation in PBS (28 days at 20°C): | Up to 20% |
| Elongation at Break (dry state): | 20 - 45% |
| Elastic Modulus (dry state): | 400 - 1300 kPa |
| Ultimate Tensile Strength (dry state): | 150 - 230 kPa |
| Manufacturer: | Gelatex Technologies |
| Country of Origin: | Estonia |
Evaluated with HaloScreening
| Cell Seeding: | 4000 to 64000 / well |
|
Cell Line: |
MDA MB 231, U2OS, Hela, Huh7, HEK293, MCF-7, HFF-1, Hekn |
| Culture Type: | Monoculture and coculture |
| Viability Assay: | WST-8, XTT, MTS |
| % Variability (8 wells): | 2% - 10% |
| Z' factor: | ≥ 0.60 |
| Fluorescence Staining: | Calcein, Propidium iodide, Phalloidin, etc. |
| Drug Screening: | DOX in MBDA MB 231 |
Figure 2: Cells stained with Calcein/PI in HaloScreening.
Resources
Related Products
Ilex Life Sciences LLC is an authorized distributor of Gelatex Technologies' Gelacell™ products.