ISOkine® FGF-Basic (FGF-2, bFGF) Human, Animal-Free Recombinant Protein, cat. no. IK0200, manufactured by ORF Genetics and distributed by Ilex Life Sciences.

ISOkine® FGF-Basic (FGF-2, bFGF) Human, Animal-Free Recombinant Protein

IK0200-0010
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Description

ISOkine® FGF-Basic (FGF-2, bFGF) Human, Animal-Free Recombinant Protein is a high-purity, bioactive growth factor expressed in barley grain and provided as a lyophilized powder. The protein produced under ISO 9001:2015 conditions contains 146 amino acids and an N-terminal 6 amino acid histidine tag for a total length of 152 amino acids and has a predicted molecular mass of 17.2 kDa.

ISOkine® growth factors and cytokines are animal-free (AOF/ACF), serum-free, and void of antibiotics, endotoxins, viruses, and prions.

Summary

FGF-basic (bFGF or FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members are heparin-binding multifunctional proteins and are involved in a variety of biological processes including angiogenesis, wound healing, and embryonic development. FGF-2 is known to play a key role during prenatal development and postnatal growth and regeneration of tissues by promoting cellular proliferation and differentiation. FGF-2 has been recognized as a critical component of human embryonic stem cell culture medium due to its vital role for cells to remain in an undifferentiated state. FGF-2 signals through FGFR 1b, 1c, 2c, 3c and 4.

Source

Expression Host: Produced in the endosperm tissue of barley grain (Hordeum vulgare) plants.

Manufacturer: ORF Genetics

Country of Origin: Iceland

Formulation

2xPBS pH 7.2, sterile filtered through 0.22 µm filter

Purity

Greater than 95% by SDS-PAGE gel analysis

Reconstitution

Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).

Stability

The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Biological Activity

Each batch of ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine® recombinant human FGF-2 is assayed by measuring its dose-dependent effect on the proliferation of 3T3 cells. The ED50 for this effect using FGF-2 is typically <0.6 ng/ml. Optimal concentration should be determined for specific applications and cell lines.

The bioactivity of ISOkine® FGF-basic is assayed by measuring its dose dependent effect on proliferation of 3T3 cells.&nbsp;The ED50 for this effect using FGFb146 is &lt; 0.4 ng/ml, corresponding to a specific activity &gt; 2.5 x10e6 U/mg.&nbsp;We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine® growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.

Endotoxin Level

Endotoxin level is < 0.01 EU per μg of ISOkine® product (< 0.01 EU/μg) by LAL method.

Pyrogen Free

Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha, and IL-1 beta induction.

Usage

This product is intended for laboratory research use only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.

Synonyms

Fibroblast Growth Factor-2, FGF2, FGF-2, FGF basic, FGF-b, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2.

Documents

Product References

  • Stokking, Martha, et al. “3D-Printed Collagen Scaffold and Adipose Tissue Mesenchymal Stem Cells-Derived Limbal Stem Cells for the Treatment of Limbal Stem Cell Deficiency.” International Journal of Bioprinting, vol. 0, no. 0, 8 Sept. 2025, p. 025290293, https://doi.org/10.36922/ijb025290293. Accessed 27 Nov. 2025.
  • Cadenas-Martin, Marta et al. “Derivation of Limbal Stem Cells from Human Adult Mesenchymal Stem Cells for the Treatment of Limbal Stem Cell Deficiency.” International journal of molecular sciences vol. 24,3 2350. 25 Jan. 2023, doi:10.3390/ijms24032350
  • Tavasoli, Mahtab et al. “Mechanism of action and therapeutic route for a muscular dystrophy caused by a genetic defect in lipid metabolism.” Nature communications vol. 13,1 1559. 23 Mar. 2022, doi:10.1038/s41467-022-29270-z
  • Pekec, Tina et al. “Ferritin-mediated iron detoxification promotes hypothermia survival in Caenorhabditis elegans and murine neurons.” Nature communications vol. 13,1 4883. 19 Aug. 2022, doi:10.1038/s41467-022-32500-z
  • D'Agostino, Stefania et al. “Rhabdomyosarcoma Cells Produce Their Own Extracellular Matrix With Minimal Involvement of Cancer-Associated Fibroblasts: A Preliminary Study.” Frontiers in oncology vol. 10 600980. 29 Jan. 2021, doi:10.3389/fonc.2020.600980
  • Szojka, Alexander R A et al. “Mechano-Hypoxia Conditioning of Engineered Human Meniscus.” Frontiers in bioengineering and biotechnology vol. 9 739438. 3 Sep. 2021, doi:10.3389/fbioe.2021.739438
  • López-Iglesias, Pilar et al. “Hypoxia induces pluripotency in primordial germ cells by HIF1α stabilization and Oct4 deregulation.” Antioxidants & redox signaling vol. 22,3 (2015): 205-23. doi:10.1089/ars.2014.5871

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Ilex Life Sciences LLC is an authorized worldwide distributor of ISOkine® growth factors & cytokines manufactured by ORF Genetics Ltd.