Lyophilized bioactive FGF-2 from Ilex Life Sciences

Fibroblast Growth Factor 2 (FGF-basic) Human, E. coli Recombinant Protein (154 aa)

A32180010
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$125.00
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$125.00
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Available January 20th
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Description

Our bioactive Fibroblast Growth Factor 2 (FGF-basic) Human, E. coli Recombinant Protein (154 aa) is a single, non-glycosylated, polypeptide chain containing 154 amino acids and has a molecular mass of 17.2 kDa. The FGF-basic is purified by proprietary chromatographic techniques.

Summary

FGF-basic, a member of the fibroblast growth factor (FGF) family, is a non-glycosylated, heparin-binding growth factor and signaling protein encoded by the FGF2 gene. It binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins. Like other FGF family members, FGF-basic possesses broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.

Additionally, FGF-basic is a critical component of human embryonic stem cell (hESCs) culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state. As a result, FGF-basic has been used to develop chemically-defined, serum-free and feeder-free hESCs culture mediums.

Other Names

Fibroblast Growth Factor-2, FGF2, FGF-2, FGF basic, FGF-b, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2.

Amino Acid Sequence

AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.

Source

E. coli

Formulation

Sterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) solution in 20mM Tris-HCl, pH7.4 and 1M NaCl.

Purity

Greater than 98.0% as determined by Analysis by SDS-PAGE.

Biological Activity

The ED50, calculated by the dose-dependent proliferation of murine balb/c 3T3 cells is <0.1 ng/ml, corresponding to a specific activity of greater than 1.0x107 Units/mg.

Reconstitution

It is recommended to reconstitute the lyophilized FGF-basic in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Shipping

At ambient temperature.  Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Lyophilized FGF-basic, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, FGF-basic should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Usage

This product is intended for Laboratory Research Use Only. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive.

Resources

Want to learn more about FGF-basic? We have compiled the links below which contain information that you may find interesting.